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The map label for this gene is mexA [H]

Identifier: 39997791

GI number: 39997791

Start: 2977070

End: 2978272

Strand: Reverse

Name: mexA [H]

Synonym: GSU2697

Alternate gene names: 39997791

Gene position: 2978272-2977070 (Counterclockwise)

Preceding gene: 39997792

Following gene: 39997790

Centisome position: 78.09

GC content: 63.34

Gene sequence:

>1203_bases
ATGCAACCCATGTACAGAACACGACTGATGACCGTAGTGGGACTTCTTGCGGTCGGGATAATGATGGTCGGTTGCGGCAA
GAAGAACGCTGCAACTCCCCCTGCCAGCGGCCCTCCCGAAGTGGGGGTGATCGTTGTCAAGCCTGAGCGCGTTGCCCTGA
CCACGGAATTGCCGGGCCGGACGTCGCCGCTTCTGATTGCCGAAGTTCGCCCCCAGGTAAGTGGGATCATCCAGAAGCGC
CTGTTTGTCGAAGGTTCCGACGTGAAGGCGGGGCAGGTCCTCTACCAGATTGACCCGGCCACGTACCAGGCGGCCTTTGC
CAGTGCCAAGGCAGCCCTTGCCCGGGCCGAGGCCAATCTCATTCCGGCACGGCTGAAGGAAGAGCGCTTCCGTGACCTGG
TCAAGATCAAAGCCGTGAGCCAGCAGGACTATGACGACGCCAATGCCGCCCTCAAACAGGCCGAAGCCGACGTGGCTTCC
GCCAAGGCTGCGGTGGAGACGGCCCGGATCAATCTGGCCTACACGAAGGTGACCGCCCCTATCTCCGGCCGCATCGGCCG
CTCGGCCATCACCGACGGGGCACTGGTAACCGCCAATCAGGCCACGGCCCTGGCCACCATCCAACAGCTCAGCCCTATCT
ATGTGGATGTCACCCAGTCCAGCGCCGAGTTGCTGAAGCTGAAGCAGAATCTGGCCAGCGGCATTATGAAGGACAACGCC
GCGGCCCAGGCCAAGGTCAAGCTCCTGCTGGAAGACGGTAGCCCTTATCCGTTGCCGGGAACGCTGAAGTTTTCGGAAGT
GACCGTTGACCAGAGCACCGGCTCAATCACCCTGCGGGCGGTTTTCCCCAACCCGAAGCAGACCCTGCTCCCCGGCATGT
TCGTGCGCGCCATCGTGGAGGAGGGGGTGAGCGACCAGGCGATGTTGGTTCCGCAGCGGGGTGTGACCCGCAATCCCCAA
GGTGAGGCCATGGTCCTGGTGGTGGGGGCCGAGGAGAAGGTGGAGCCGCGGGTAATCAAGGTCGTCCGGACCGTGGGGGA
GAACTGGCTGGTGAGCGACGGCCTGAAAGCCGGCGACCGGGTCATTCTGGAGGGAATCCAGAAGGCACGGCCGGGCACGC
AGGTTAAAACCGTGCCGTTCGCGGCAAAACCGGAGGCTGCTGCCCCCGCGGCAGCGAACCAAGCGGCGAGTGCCAAAAAA
TAG

Upstream 100 bases:

>100_bases
ACCATGGCGGTCTGGGCCCTGACGGCTATTATCGCTTTTTTGGGGAAAAACAAGTAACTGGCCGTGAGGCCGGCAATTTA
TGAACAGTATCGAGGTGTGT

Downstream 100 bases:

>100_bases
GCTGACAGCGTTGCGATCCTAAGGCCCCGCCTCCCTGTCACCACGGGGGAATACGCATTAAGGAGCTTCTTTCATGTCCC
GCTTTTTCATAAACAGACCA

Product: multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQPMYRTRLMTVVGLLAVGIMMVGCGKKNAATPPASGPPEVGVIVVKPERVALTTELPGRTSPLLIAEVRPQVSGIIQKR
LFVEGSDVKAGQVLYQIDPATYQAAFASAKAALARAEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVAS
AKAAVETARINLAYTKVTAPISGRIGRSAITDGALVTANQATALATIQQLSPIYVDVTQSSAELLKLKQNLASGIMKDNA
AAQAKVKLLLEDGSPYPLPGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEEGVSDQAMLVPQRGVTRNPQ
GEAMVLVVGAEEKVEPRVIKVVRTVGENWLVSDGLKAGDRVILEGIQKARPGTQVKTVPFAAKPEAAAPAAANQAASAKK

Sequences:

>Translated_400_residues
MQPMYRTRLMTVVGLLAVGIMMVGCGKKNAATPPASGPPEVGVIVVKPERVALTTELPGRTSPLLIAEVRPQVSGIIQKR
LFVEGSDVKAGQVLYQIDPATYQAAFASAKAALARAEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVAS
AKAAVETARINLAYTKVTAPISGRIGRSAITDGALVTANQATALATIQQLSPIYVDVTQSSAELLKLKQNLASGIMKDNA
AAQAKVKLLLEDGSPYPLPGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEEGVSDQAMLVPQRGVTRNPQ
GEAMVLVVGAEEKVEPRVIKVVRTVGENWLVSDGLKAGDRVILEGIQKARPGTQVKTVPFAAKPEAAAPAAANQAASAKK
>Mature_400_residues
MQPMYRTRLMTVVGLLAVGIMMVGCGKKNAATPPASGPPEVGVIVVKPERVALTTELPGRTSPLLIAEVRPQVSGIIQKR
LFVEGSDVKAGQVLYQIDPATYQAAFASAKAALARAEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVAS
AKAAVETARINLAYTKVTAPISGRIGRSAITDGALVTANQATALATIQQLSPIYVDVTQSSAELLKLKQNLASGIMKDNA
AAQAKVKLLLEDGSPYPLPGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEEGVSDQAMLVPQRGVTRNPQ
GEAMVLVVGAEEKVEPRVIKVVRTVGENWLVSDGLKAGDRVILEGIQKARPGTQVKTVPFAAKPEAAAPAAANQAASAKK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=383, Percent_Identity=58.4856396866841, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=57.5757575757576, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=49.6, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=28.9230769230769, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87081796, Length=205, Percent_Identity=30.7317073170732, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081951, Length=117, Percent_Identity=35.042735042735, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42226; Mature: 42226

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPMYRTRLMTVVGLLAVGIMMVGCGKKNAATPPASGPPEVGVIVVKPERVALTTELPGR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCC
TSPLLIAEVRPQVSGIIQKRLFVEGSDVKAGQVLYQIDPATYQAAFASAKAALARAEANL
CCCEEEEECCHHHHHHHHHHHEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCC
IPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKAAVETARINLAYTKVTAP
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECC
ISGRIGRSAITDGALVTANQATALATIQQLSPIYVDVTQSSAELLKLKQNLASGIMKDNA
CCCCCCCHHCCCCEEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
AAQAKVKLLLEDGSPYPLPGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVE
CCEEEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHH
EGVSDQAMLVPQRGVTRNPQGEAMVLVVGAEEKVEPRVIKVVRTVGENWLVSDGLKAGDR
CCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCH
VILEGIQKARPGTQVKTVPFAAKPEAAAPAAANQAASAKK
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MQPMYRTRLMTVVGLLAVGIMMVGCGKKNAATPPASGPPEVGVIVVKPERVALTTELPGR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCC
TSPLLIAEVRPQVSGIIQKRLFVEGSDVKAGQVLYQIDPATYQAAFASAKAALARAEANL
CCCEEEEECCHHHHHHHHHHHEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCC
IPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKAAVETARINLAYTKVTAP
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECC
ISGRIGRSAITDGALVTANQATALATIQQLSPIYVDVTQSSAELLKLKQNLASGIMKDNA
CCCCCCCHHCCCCEEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
AAQAKVKLLLEDGSPYPLPGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVE
CCEEEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHH
EGVSDQAMLVPQRGVTRNPQGEAMVLVVGAEEKVEPRVIKVVRTVGENWLVSDGLKAGDR
CCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCH
VILEGIQKARPGTQVKTVPFAAKPEAAAPAAANQAASAKK
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]