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The map label for this gene is rpfG [H]

Identifier: 39997669

GI number: 39997669

Start: 2835091

End: 2836197

Strand: Reverse

Name: rpfG [H]

Synonym: GSU2574

Alternate gene names: 39997669

Gene position: 2836197-2835091 (Counterclockwise)

Preceding gene: 39997670

Following gene: 39997668

Centisome position: 74.36

GC content: 63.23

Gene sequence:

>1107_bases
ATGCTGGATTCAATCTCGGTCCTTGTGGTAGATGATGAAGAAATGAACCGTGAGTTGCTTTCGGTCATGCTCGACGGCCT
GGCAACGCCGATCACGGCGCAGAATGGCCTGGAAGCTCTCCGGGTGCTGGATAAGAACTCCGCCATCGACCTCATCCTGC
TCGATCTGGAGATGCCCGAGCTGAACGGGTTTGCGACGCTCGCCACCCTCAAGAAGCATCCTCTGTGGTGCGACATCCCC
GTTATCGTCCTCACGGCCAGCAAGGACGAAGTGACCCGGACCCTGAGGCTGGGGGCCAACGATTTTCTCGCCAAACCCTA
CGACACGGAGGAGTTGCGCCTGCGGGTGATGAACCATGTCCGCGCCAAGAAGCTGGTGGACCTGTCCCAGGACGTGAACG
GCATCCTGGAGCGGGAAGTGGCCCGCAAGACCGCCGAGGTCAGAAACGCCCTGGCCATGGCCCGGGAGGCGGAGTACGAG
ATAACGCTGCGCCTTGGCCGCGCCGCCGAGTTCCGCGATGTGGAAACCGGCATGCACACGCGCCGCATCAGCGAGATGGC
CCGGGAGCTCGGCCGGTTGAGCGGGCTGCCCGAGGACCAGTGCGAGGTGCTCCGCTACGCGGCTCCGCTTCACGACGTGG
GCAAGCTCGGCATACCGGACCGGATACTCCTCAAGCCCGGACGGCTCACGGACGAGGAGATGGAGATCATGCGTCTCCAC
ACGGTCATCGGCGGCAAGATCCTCGCCGGCGGCGACCGGTACACATCCCTGGAGATGGGACGGATCGTTGCCCTCCAGCA
CCACGAAAAATGGGACGGCACCGGTTACCCCTGCGGGTTGGCCGGAGAAGATATCCACATCTTCGGGCGCATCGTCATGA
TCGTCGATGTGTTCGACGCCCTCTGCTCGGAGCGCCCCTACAAGCCGGCAATGCCCTTCGACGAAGTTCTCTCCGTCATG
GGGGACGGGCGGGCATCGTTCTTCGACCCTCGTCTGCTGGAAATATTCCTGGCCAACGTGGAGGCCTTCGTCGCGATCCG
GACCTCGCTCATGGACCAGCCCGTGGAGGAGAAGGCGGCCCTGGAGCAGGTGCTGTTCCTGGCATGA

Upstream 100 bases:

>100_bases
GGACGCCGCCATCAGCGGCCGGCTGGACGAAGCGGTTTCCCTGCTTCAGCCCCTTGCCGCCGAATATGACACGTTTGTAC
GCGAAACGGAGAGATAGATC

Downstream 100 bases:

>100_bases
TCGCGGCCTGAACGGGAAGGGAGTGGAATGGAAGAGCAGCGCCTGTCAATCCTGATCGGCCGCTTTACCGCGGCGGCCAC
CGCCCACGGGGAAGCCCTGG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLDSISVLVVDDEEMNRELLSVMLDGLATPITAQNGLEALRVLDKNSAIDLILLDLEMPELNGFATLATLKKHPLWCDIP
VIVLTASKDEVTRTLRLGANDFLAKPYDTEELRLRVMNHVRAKKLVDLSQDVNGILEREVARKTAEVRNALAMAREAEYE
ITLRLGRAAEFRDVETGMHTRRISEMARELGRLSGLPEDQCEVLRYAAPLHDVGKLGIPDRILLKPGRLTDEEMEIMRLH
TVIGGKILAGGDRYTSLEMGRIVALQHHEKWDGTGYPCGLAGEDIHIFGRIVMIVDVFDALCSERPYKPAMPFDEVLSVM
GDGRASFFDPRLLEIFLANVEAFVAIRTSLMDQPVEEKAALEQVLFLA

Sequences:

>Translated_368_residues
MLDSISVLVVDDEEMNRELLSVMLDGLATPITAQNGLEALRVLDKNSAIDLILLDLEMPELNGFATLATLKKHPLWCDIP
VIVLTASKDEVTRTLRLGANDFLAKPYDTEELRLRVMNHVRAKKLVDLSQDVNGILEREVARKTAEVRNALAMAREAEYE
ITLRLGRAAEFRDVETGMHTRRISEMARELGRLSGLPEDQCEVLRYAAPLHDVGKLGIPDRILLKPGRLTDEEMEIMRLH
TVIGGKILAGGDRYTSLEMGRIVALQHHEKWDGTGYPCGLAGEDIHIFGRIVMIVDVFDALCSERPYKPAMPFDEVLSVM
GDGRASFFDPRLLEIFLANVEAFVAIRTSLMDQPVEEKAALEQVLFLA
>Mature_368_residues
MLDSISVLVVDDEEMNRELLSVMLDGLATPITAQNGLEALRVLDKNSAIDLILLDLEMPELNGFATLATLKKHPLWCDIP
VIVLTASKDEVTRTLRLGANDFLAKPYDTEELRLRVMNHVRAKKLVDLSQDVNGILEREVARKTAEVRNALAMAREAEYE
ITLRLGRAAEFRDVETGMHTRRISEMARELGRLSGLPEDQCEVLRYAAPLHDVGKLGIPDRILLKPGRLTDEEMEIMRLH
TVIGGKILAGGDRYTSLEMGRIVALQHHEKWDGTGYPCGLAGEDIHIFGRIVMIVDVFDALCSERPYKPAMPFDEVLSVM
GDGRASFFDPRLLEIFLANVEAFVAIRTSLMDQPVEEKAALEQVLFLA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=35, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=35.3846153846154, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41142; Mature: 41142

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSISVLVVDDEEMNRELLSVMLDGLATPITAQNGLEALRVLDKNSAIDLILLDLEMPE
CCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
LNGFATLATLKKHPLWCDIPVIVLTASKDEVTRTLRLGANDFLAKPYDTEELRLRVMNHV
CCCHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
RAKKLVDLSQDVNGILEREVARKTAEVRNALAMAREAEYEITLRLGRAAEFRDVETGMHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
RRISEMARELGRLSGLPEDQCEVLRYAAPLHDVGKLGIPDRILLKPGRLTDEEMEIMRLH
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
TVIGGKILAGGDRYTSLEMGRIVALQHHEKWDGTGYPCGLAGEDIHIFGRIVMIVDVFDA
HHHCCEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LCSERPYKPAMPFDEVLSVMGDGRASFFDPRLLEIFLANVEAFVAIRTSLMDQPVEEKAA
HHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LEQVLFLA
HHHHHHCC
>Mature Secondary Structure
MLDSISVLVVDDEEMNRELLSVMLDGLATPITAQNGLEALRVLDKNSAIDLILLDLEMPE
CCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
LNGFATLATLKKHPLWCDIPVIVLTASKDEVTRTLRLGANDFLAKPYDTEELRLRVMNHV
CCCHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
RAKKLVDLSQDVNGILEREVARKTAEVRNALAMAREAEYEITLRLGRAAEFRDVETGMHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
RRISEMARELGRLSGLPEDQCEVLRYAAPLHDVGKLGIPDRILLKPGRLTDEEMEIMRLH
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
TVIGGKILAGGDRYTSLEMGRIVALQHHEKWDGTGYPCGLAGEDIHIFGRIVMIVDVFDA
HHHCCEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LCSERPYKPAMPFDEVLSVMGDGRASFFDPRLLEIFLANVEAFVAIRTSLMDQPVEEKAA
HHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LEQVLFLA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]