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The map label for this gene is topA [H]

Identifier: 39997644

GI number: 39997644

Start: 2810320

End: 2812593

Strand: Reverse

Name: topA [H]

Synonym: GSU2549

Alternate gene names: 39997644

Gene position: 2812593-2810320 (Counterclockwise)

Preceding gene: 39997645

Following gene: 39997643

Centisome position: 73.74

GC content: 62.53

Gene sequence:

>2274_bases
ATGTCCCAACATCTCGTCATAGTAGAATCTCCTGCCAAGGCTAAGACCATAGAGAAGTTCCTCGGCCCGGACTACAAGGT
GCTCGCATCCTACGGCCATGTGCGCGCCCTGCCGAGCAAGCAGGGCTCCGTGGACGTGGAGCACGACTTCGAGCCCCGCT
ACGCCGTCCTGCCCGAGAGCAAACGGCACATCGACGCCATCAAGAAGGAGTTGAAGGCGAGCGATTCGCTCCTGCTGGCC
ACCGACCCCGACCGGGAAGGGGAGGCCATCTCCTGGCACCTGCTGGCGGCCCTGGGCGTGAAGCCCGAGAAACCGCCGGT
ACCGGTCAGGCGGGTGGTGTTCCACGAGATCACCAAGGACGCCATAGTCCATGCCGTGGAGAATCCCCGCGATATCTCAC
AGGATCTGGTGGACGCCCAGCAGGCTCGCTCAATTCTCGATTATCTCGTGGGTTTCAATCTCTCCCCCTTCCTCTGGAAG
AAGATTCGTTACGGCCTTTCCGCCGGCCGGGTCCAGTCGGTGGCCCTGCGGCTCATCTGCGAGCGGGAGAAGGAGATCCA
GGCGTTCCAGTCCCAGGAATACTGGACCATCGGCGCGGAGCTGGCCAAGGAGGGGGGGCAGAAGTGCACCGCCAATCTGG
TCGAAGCCGAGGGGAAGAAGCTCGACAAGTTCGACATCCCCGATCAGGCTGCGGCCGACCGGCTCGTGAAGGCCCTGGAG
AACGCCACCTTCACTGTGGACAAGGTGACGAAGAGCGAGCGCAAGCGGACGCCGGCGCCGCCGTTTACCACATCGACCCT
CCAGCAGGAGGCTGCCCGCAAACTGGGCTTTTCGGCCAAAAAGACCATGGCCACGGCCCAAAAGCTCTACGAAGGGGTCG
CCATCGACGAAGGGCTCGTGGGTCTCATCACTTACATGCGTACCGACAGCGTGGTGCTGTCGAACCAGGCACTGCAGGAG
GCCCACCAGGTCATCACTTCCCTGTACGGTCCCGAATACGCCCTTGCCAAGCCCCGCTTCTACAAAAACAAGGCTAAGAA
CGCCCAGGAGGCCCACGAGGCGGTCCGCCCCACCTCCATCGCCCGCACCCCGGCGGAGCTGAAAAAGTACCTCTCCTCCG
ACCAGTTCAAGCTGTACGACCTGATCTGGAAGCGGACCGTGGCCTGCCAGATGGCCGAGGCGCTCCTGGACCAAACCTCC
GTCGATATCGGCGCGGGCAAGGGCTACCGCTTCCGGGCCGCCGGCACCGTGATCCGCTTTCCCGGATTTATGAAGCTGTA
CATCGAAGGGGTGGACGATCAGGCCGAAGAGAAGGAGGGGACCCTCCCTCCCCTCACCGAAGGGGAACTCCTGAAGCTCC
TGAAGCTCCTCCCGGAGCAGCACTTCACCCAGCCGCCCCCCCGGTACACCGAGGCGAGCCTGGTGAAGACGCTGGAAGAG
TACGGCATCGGGCGCCCCTCAACCTATGCCTCCATCATGAACACGCTCCTGGAGCGCAAGTACGCCCGCCTCGACAGCAA
GCGCTTCATCCCCGAGGATGTGGGGATGGTGGTCAACGATCTTCTGACCAACCATTTCACCACGTACGTGGACTACAACT
TCACCGCCACCCTTGAGGAAGAGCTCGACCAGGTCTCCCGGGGGGAAAAGCGGTGGAAGCCGCTGCTGCGCGAGTTCTGG
GAGCCCTTCCAGGGACTGCTCAAACAGAAAGAGGGCGAGGTCAGCAAGGCGGACCTCACCACCGAGGCCACGGACGAGGC
ATGCCCCGAATGCGGAAAACCCCTGGTGGTGAAGCTCGGCAAGCGCGGCAAGTTCATTGCCTGCTCCGGTTACAAGGAAG
GGTGCACCTATACCCGCAACATCGACCAGGGTGAGGGAAGAGAGCAGGCGGAGCCGGTCCTGTCCGAGGAAAAGTGCGAC
AAATGCGGCAGCCCCATGCTCATCAAGGACGGGCGCTTCGGCAAGTACCTGGCCTGCTCGGCCTATCCCGCCTGCAAGAA
CATCCAGCCCCTGGTGAAGCCCAAGGGGACCGGCCATACCTGCCCCGAATGCAAGGAAGGGGAGCTGACCGAGAAAAAGT
CCCGCTACGGCAAGATGTTCTACTCCTGCAACCGCTATCCCCAGTGCAAGTTCGCCCTCTGGGACCCGCCCCAGCCGGGG
CCGTGCCCCAAGTGCGGCTTCCCGCTGCTGGTGAAGAAGGTCTACAAGCGGGAAGGGGAGTTCCTCAAGTGTCCCAAGGA
AGGATGCGACTACCGGACCGAAGGGAAAAAGTAA

Upstream 100 bases:

>100_bases
TGGGGGAGGTTTCGGCTATGTTACTGCGCCTGGAACTGAAAGGAGTCGTAACTCAGCTTCCCGGCAAGTTTTTTCACGCT
AACTGACAACGGTACCGTCT

Downstream 100 bases:

>100_bases
TCACGTCGCCGACCGGGCGGGGAGCGATCCCCGCCTTTTTCTCGCACGATGTGCGGCTGCTGAAGGAAATCCATGATGCT
TACCGACATGCACGAAACCT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MSQHLVIVESPAKAKTIEKFLGPDYKVLASYGHVRALPSKQGSVDVEHDFEPRYAVLPESKRHIDAIKKELKASDSLLLA
TDPDREGEAISWHLLAALGVKPEKPPVPVRRVVFHEITKDAIVHAVENPRDISQDLVDAQQARSILDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLICEREKEIQAFQSQEYWTIGAELAKEGGQKCTANLVEAEGKKLDKFDIPDQAAADRLVKALE
NATFTVDKVTKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMATAQKLYEGVAIDEGLVGLITYMRTDSVVLSNQALQE
AHQVITSLYGPEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPAELKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTS
VDIGAGKGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLLKLLPEQHFTQPPPRYTEASLVKTLEE
YGIGRPSTYASIMNTLLERKYARLDSKRFIPEDVGMVVNDLLTNHFTTYVDYNFTATLEEELDQVSRGEKRWKPLLREFW
EPFQGLLKQKEGEVSKADLTTEATDEACPECGKPLVVKLGKRGKFIACSGYKEGCTYTRNIDQGEGREQAEPVLSEEKCD
KCGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPG
PCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK

Sequences:

>Translated_757_residues
MSQHLVIVESPAKAKTIEKFLGPDYKVLASYGHVRALPSKQGSVDVEHDFEPRYAVLPESKRHIDAIKKELKASDSLLLA
TDPDREGEAISWHLLAALGVKPEKPPVPVRRVVFHEITKDAIVHAVENPRDISQDLVDAQQARSILDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLICEREKEIQAFQSQEYWTIGAELAKEGGQKCTANLVEAEGKKLDKFDIPDQAAADRLVKALE
NATFTVDKVTKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMATAQKLYEGVAIDEGLVGLITYMRTDSVVLSNQALQE
AHQVITSLYGPEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPAELKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTS
VDIGAGKGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLLKLLPEQHFTQPPPRYTEASLVKTLEE
YGIGRPSTYASIMNTLLERKYARLDSKRFIPEDVGMVVNDLLTNHFTTYVDYNFTATLEEELDQVSRGEKRWKPLLREFW
EPFQGLLKQKEGEVSKADLTTEATDEACPECGKPLVVKLGKRGKFIACSGYKEGCTYTRNIDQGEGREQAEPVLSEEKCD
KCGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPG
PCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
>Mature_756_residues
SQHLVIVESPAKAKTIEKFLGPDYKVLASYGHVRALPSKQGSVDVEHDFEPRYAVLPESKRHIDAIKKELKASDSLLLAT
DPDREGEAISWHLLAALGVKPEKPPVPVRRVVFHEITKDAIVHAVENPRDISQDLVDAQQARSILDYLVGFNLSPFLWKK
IRYGLSAGRVQSVALRLICEREKEIQAFQSQEYWTIGAELAKEGGQKCTANLVEAEGKKLDKFDIPDQAAADRLVKALEN
ATFTVDKVTKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMATAQKLYEGVAIDEGLVGLITYMRTDSVVLSNQALQEA
HQVITSLYGPEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPAELKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTSV
DIGAGKGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLLKLLPEQHFTQPPPRYTEASLVKTLEEY
GIGRPSTYASIMNTLLERKYARLDSKRFIPEDVGMVVNDLLTNHFTTYVDYNFTATLEEELDQVSRGEKRWKPLLREFWE
PFQGLLKQKEGEVSKADLTTEATDEACPECGKPLVVKLGKRGKFIACSGYKEGCTYTRNIDQGEGREQAEPVLSEEKCDK
CGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPGP
CPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=583, Percent_Identity=25.7289879931389, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI4507635, Length=740, Percent_Identity=22.1621621621622, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787529, Length=772, Percent_Identity=38.9896373056995, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994931, Length=160, Percent_Identity=40.625, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788061, Length=547, Percent_Identity=25.5941499085923, Blast_Score=132, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=548, Percent_Identity=26.8248175182482, Blast_Score=135, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323263, Length=501, Percent_Identity=23.75249500998, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585251, Length=683, Percent_Identity=24.7437774524158, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 84968; Mature: 84837

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHLVIVESPAKAKTIEKFLGPDYKVLASYGHVRALPSKQGSVDVEHDFEPRYAVLPES
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEECCCC
KRHIDAIKKELKASDSLLLATDPDREGEAISWHLLAALGVKPEKPPVPVRRVVFHEITKD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AIVHAVENPRDISQDLVDAQQARSILDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLIC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIQAFQSQEYWTIGAELAKEGGQKCTANLVEAEGKKLDKFDIPDQAAADRLVKALE
HHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
NATFTVDKVTKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMATAQKLYEGVAIDEGLV
CCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
GLITYMRTDSVVLSNQALQEAHQVITSLYGPEYALAKPRFYKNKAKNAQEAHEAVRPTSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCHHHHHHHHCCHHH
ARTPAELKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTSVDIGAGKGYRFRAAGTVIRF
HCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEC
PGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLLKLLPEQHFTQPPPRYTEASLVKTLEE
CCEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHH
YGIGRPSTYASIMNTLLERKYARLDSKRFIPEDVGMVVNDLLTNHFTTYVDYNFTATLEE
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCEEEHHHH
ELDQVSRGEKRWKPLLREFWEPFQGLLKQKEGEVSKADLTTEATDEACPECGKPLVVKLG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHCCCEEEEEC
KRGKFIACSGYKEGCTYTRNIDQGEGREQAEPVLSEEKCDKCGSPMLIKDGRFGKYLACS
CCCCEEEECCCCCCCCEECCCCCCCCHHHHCCHHCHHHHHHCCCCEEEECCCCCCEEEEC
AYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
CCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCCC
>Mature Secondary Structure 
SQHLVIVESPAKAKTIEKFLGPDYKVLASYGHVRALPSKQGSVDVEHDFEPRYAVLPES
CCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEECCCC
KRHIDAIKKELKASDSLLLATDPDREGEAISWHLLAALGVKPEKPPVPVRRVVFHEITKD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AIVHAVENPRDISQDLVDAQQARSILDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLIC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIQAFQSQEYWTIGAELAKEGGQKCTANLVEAEGKKLDKFDIPDQAAADRLVKALE
HHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
NATFTVDKVTKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMATAQKLYEGVAIDEGLV
CCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
GLITYMRTDSVVLSNQALQEAHQVITSLYGPEYALAKPRFYKNKAKNAQEAHEAVRPTSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCHHHHHHHHCCHHH
ARTPAELKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTSVDIGAGKGYRFRAAGTVIRF
HCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEC
PGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLLKLLPEQHFTQPPPRYTEASLVKTLEE
CCEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHH
YGIGRPSTYASIMNTLLERKYARLDSKRFIPEDVGMVVNDLLTNHFTTYVDYNFTATLEE
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCEEEHHHH
ELDQVSRGEKRWKPLLREFWEPFQGLLKQKEGEVSKADLTTEATDEACPECGKPLVVKLG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHCCCEEEEEC
KRGKFIACSGYKEGCTYTRNIDQGEGREQAEPVLSEEKCDKCGSPMLIKDGRFGKYLACS
CCCCEEEECCCCCCCCEECCCCCCCCHHHHCCHHCHHHHHHCCCCEEEECCCCCCEEEEC
AYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
CCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA