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The map label for this gene is gid

Identifier: 39997642

GI number: 39997642

Start: 2808536

End: 2809840

Strand: Reverse

Name: gid

Synonym: GSU2547

Alternate gene names: 39997642

Gene position: 2809840-2808536 (Counterclockwise)

Preceding gene: 39997643

Following gene: 39997641

Centisome position: 73.67

GC content: 66.51

Gene sequence:

>1305_bases
ATGACCGGTGCCGTCACCATCATCGGCGGCGGCCTGGCTGGGTGCGAGGCCGCCTGGCAGATCGCCGGGCGCGGCGTGCG
GGTCGTTCTGCGGGAGATGAAGCCCCAGCGCTACTCGCCGGCCCACCACCTGTCCGGGCTGGCGGAGCTGGTCTGCTCAA
ACTCGCTCCGGGGGGAGTCCCTGGAGAACGCCGTGGGGCTCCTCAAGGAAGAGTTGCGGCGGGCCGGCTCACTCATCATG
GCCGCGGCCGACGCCACCCGGGTGCCTGCAGGCGGCGCCCTGGCCGTGGACCGGGAACTGTTCTCCTCCTACGTAACCGA
GCGGATCGAGGGGCATCCCCTGATCGAGCTGGTGCGCGAGGAGGTGACGGAGCTTCCCGCCGAAGGGGTCCTGGTGATCG
CCTCGGGGCCCCTGACCAGCGACGCCCTGGCCGAGCGGCTGAAGCAGATCACCGGCGACAGCCTCTACTTCTACGATGCC
ATCGCTCCCATCGTTGCGGCCGACTCCCTCGATTCGGACAAGGTTTTCCGGGCCTCCCGCTACGGCAAGGGGGACGGTGA
CGACTACCTGAACTGCCCCCTCTCCGAAGAAGAGTACGAGCGGTTCGTTGATGCCGTTCTCGCCGCGGAAAAGGTACCGG
CCAGGGATTTTGAAAAAGTGGTACACTTTGAGGGGTGCATGCCCGTGGAAGAGATGGCGGAGCGGGGCCGGGAAACTCTC
CGCTTCGGCCCCCTGAAGCCGGTGGGGCTCACGGACCCCCGTACCGGCCGTGAACCCCACGCGGTGGTGCAGTTGCGGGC
CGAGAACCGGGAGGGAACCCTGTTCAACCTGGTGGGTTTCCAGACCAAGCTCACCTGGCCCGAACAGCGGCGAGTCTTCC
GAATGATCCCCGGCCTGGAGAGCGCCGAGTTCGTCCGGCTCGGCTCAATGCACCGCAATACGTTCATCAATGCGCCGACC
CTGCTGGAGCCCACCTTCCGGCTCAAGGGCGACCCCCGCACCTTCTTTGCCGGCCAGATCACCGGGGTGGAGGGATACGT
GGAATCGGCCGGCAGCGGTTTCCTGGCCGGTATCAATAGTGCCCGGCTCGTGCGGGGTGAGGATCCGGCAGTTCCGCCGC
CGGTCACCGCCCTGGGTGCGCTGGTGGCCCATATCACCACTGCGCCGGCCAGGCACTTTCAGCCTATGAACGTAAACTAC
GGACTGTTCCCGCCCCTCGAGGGGAAAGTGAAAAAGAAGGATCGCCGGGGAAGGCTTGCGGAGCGAGCCCTGGCGGAACT
GGATCATTGGCTCGCAACAGTGTAG

Upstream 100 bases:

>100_bases
GGTATCTGACCCTCTGGGACGAGGAGAAGGGAAAGGCCCGACTCCTGGCTGGCCACCCCCTCCAGAAGTTCGAGATTCCC
GCGGCGGGCGACCCGTCCCC

Downstream 100 bases:

>100_bases
GGGCGAACCTCGCGTTCGCCTTTGACGTCGCGTGACACCAGAAGCGGGCGATCACAAGGATCGCTCCCACGGGGTATCTA
TGGACGACAAGGAAAAACAG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTGAVTIIGGGLAGCEAAWQIAGRGVRVVLREMKPQRYSPAHHLSGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIM
AAADATRVPAGGALAVDRELFSSYVTERIEGHPLIELVREEVTELPAEGVLVIASGPLTSDALAERLKQITGDSLYFYDA
IAPIVAADSLDSDKVFRASRYGKGDGDDYLNCPLSEEEYERFVDAVLAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
RFGPLKPVGLTDPRTGREPHAVVQLRAENREGTLFNLVGFQTKLTWPEQRRVFRMIPGLESAEFVRLGSMHRNTFINAPT
LLEPTFRLKGDPRTFFAGQITGVEGYVESAGSGFLAGINSARLVRGEDPAVPPPVTALGALVAHITTAPARHFQPMNVNY
GLFPPLEGKVKKKDRRGRLAERALAELDHWLATV

Sequences:

>Translated_434_residues
MTGAVTIIGGGLAGCEAAWQIAGRGVRVVLREMKPQRYSPAHHLSGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIM
AAADATRVPAGGALAVDRELFSSYVTERIEGHPLIELVREEVTELPAEGVLVIASGPLTSDALAERLKQITGDSLYFYDA
IAPIVAADSLDSDKVFRASRYGKGDGDDYLNCPLSEEEYERFVDAVLAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
RFGPLKPVGLTDPRTGREPHAVVQLRAENREGTLFNLVGFQTKLTWPEQRRVFRMIPGLESAEFVRLGSMHRNTFINAPT
LLEPTFRLKGDPRTFFAGQITGVEGYVESAGSGFLAGINSARLVRGEDPAVPPPVTALGALVAHITTAPARHFQPMNVNY
GLFPPLEGKVKKKDRRGRLAERALAELDHWLATV
>Mature_433_residues
TGAVTIIGGGLAGCEAAWQIAGRGVRVVLREMKPQRYSPAHHLSGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIMA
AADATRVPAGGALAVDRELFSSYVTERIEGHPLIELVREEVTELPAEGVLVIASGPLTSDALAERLKQITGDSLYFYDAI
APIVAADSLDSDKVFRASRYGKGDGDDYLNCPLSEEEYERFVDAVLAAEKVPARDFEKVVHFEGCMPVEEMAERGRETLR
FGPLKPVGLTDPRTGREPHAVVQLRAENREGTLFNLVGFQTKLTWPEQRRVFRMIPGLESAEFVRLGSMHRNTFINAPTL
LEPTFRLKGDPRTFFAGQITGVEGYVESAGSGFLAGINSARLVRGEDPAVPPPVTALGALVAHITTAPARHFQPMNVNYG
LFPPLEGKVKKKDRRGRLAERALAELDHWLATV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=120, Percent_Identity=38.3333333333333, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_GEOSL (Q74A44)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953593.1
- ProteinModelPortal:   Q74A44
- SMR:   Q74A44
- GeneID:   2686108
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2547
- NMPDR:   fig|243231.1.peg.2531
- TIGR:   GSU2547
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   GSUL243231:GSU_2547-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 47291; Mature: 47159

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAVTIIGGGLAGCEAAWQIAGRGVRVVLREMKPQRYSPAHHLSGLAELVCSNSLRGES
CCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCH
LENAVGLLKEELRRAGSLIMAAADATRVPAGGALAVDRELFSSYVTERIEGHPLIELVRE
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
EVTELPAEGVLVIASGPLTSDALAERLKQITGDSLYFYDAIAPIVAADSLDSDKVFRASR
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHC
YGKGDGDDYLNCPLSEEEYERFVDAVLAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHE
RFGPLKPVGLTDPRTGREPHAVVQLRAENREGTLFNLVGFQTKLTWPEQRRVFRMIPGLE
ECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCEEECCCHHHHHHHHHCCCCC
SAEFVRLGSMHRNTFINAPTLLEPTFRLKGDPRTFFAGQITGVEGYVESAGSGFLAGINS
CHHHHHHCCHHHCCCCCCCCCCCCCEEECCCCCEEEEEEECCHHHHHHCCCCCEEECCCC
ARLVRGEDPAVPPPVTALGALVAHITTAPARHFQPMNVNYGLFPPLEGKVKKKDRRGRLA
CEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHCHHHHHHHH
ERALAELDHWLATV
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TGAVTIIGGGLAGCEAAWQIAGRGVRVVLREMKPQRYSPAHHLSGLAELVCSNSLRGES
CCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCH
LENAVGLLKEELRRAGSLIMAAADATRVPAGGALAVDRELFSSYVTERIEGHPLIELVRE
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
EVTELPAEGVLVIASGPLTSDALAERLKQITGDSLYFYDAIAPIVAADSLDSDKVFRASR
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHC
YGKGDGDDYLNCPLSEEEYERFVDAVLAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHE
RFGPLKPVGLTDPRTGREPHAVVQLRAENREGTLFNLVGFQTKLTWPEQRRVFRMIPGLE
ECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCEEECCCHHHHHHHHHCCCCC
SAEFVRLGSMHRNTFINAPTLLEPTFRLKGDPRTFFAGQITGVEGYVESAGSGFLAGINS
CHHHHHHCCHHHCCCCCCCCCCCCCEEECCCCCEEEEEEECCHHHHHHCCCCCEEECCCC
ARLVRGEDPAVPPPVTALGALVAHITTAPARHFQPMNVNYGLFPPLEGKVKKKDRRGRLA
CEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHCHHHHHHHH
ERALAELDHWLATV
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA