Click here to switch to the map view.

The map label for this gene is usf [H]

Identifier: 39997631

GI number: 39997631

Start: 2798512

End: 2799324

Strand: Reverse

Name: usf [H]

Synonym: GSU2536

Alternate gene names: 39997631

Gene position: 2799324-2798512 (Counterclockwise)

Preceding gene: 39997632

Following gene: 39997630

Centisome position: 73.39

GC content: 65.31

Gene sequence:

>813_bases
ATGAAACAGAGCATTCGCGCAGGACTCATGCTGGTCGTGGCGGCCCTGGTCGTGCTGGCGGCGTTTCCCGCCGCGGCCAA
GGTCCGGGGTAGGGTGGTTGAGTACCGGGACGGCACCGTCACCATGAAGGGGTACCTGGCATGGGACCCGGCCCTGAAAG
GGAAGCGTCCCGGCGTGCTGGTGGTGCACGAGTGGTGGGGGCACAACGAGTATGCCCGCAAGCGGGCCAGGATGCTGGCC
GGCCTGGGCTACACGGCCCTGGCCGTCGACATGTACGGCGAGGGAAAGCAGGCTCCCCATCCGGACGATGCGGCCGCATT
CGCCGCGGAGGTCATGAAGAACGGCAACCTGATGAAGGACCGCTTCATGGCGGCCATGAACCTGCTGAAAGACCAGCCCA
CGGTGGACCCGGGCCGGATCGGGGCCATCGGTTACTGCTTCGGCGGCGCGGTTGTGCTGAACATGGCTCGCCAGGGAGTT
GATCTGGCCGGAGTGGTGAGCTTCCATGGCAGCCTTGCCACAGACCGGCCGGCCGAGCCGGGCGGAATCAAGGCCCGCGT
CCTGGTGCTCAATGGTGCGGCAGACCGCTTCGTTCCGCCGGAGCAGGTCGGGGCGTTCGCCGCTGAAATGGCCCGGTCCG
GTGCCGAGTTCGGTTTTATTTCCTATGCCGGGGCCAAGCACAGTTTCACCAACCCCGAGGCAGACGCCTTTGCCAAACGC
TTCGGTCTCGACGTGGCCTATGACGCCCGTGCCGACCGACGGTCATGGGCGGAAATGAAGCGCTTTTTCCATCTCTGTTT
TTCCGGCGGATAA

Upstream 100 bases:

>100_bases
CACCCGCCCCCTTCCGACCGCTCGCACCCTCGATGCGGCCGGTTGAGCGTATCCGTTCGGATGGTAGAATTGGATACGTG
CAACGAATCGGGAGGTGGCC

Downstream 100 bases:

>100_bases
CCGGACCTTCGTAACGGCGGCAGGTAGCCGCACACACCGTTGCGCCCCGAGAGAGGCCAGGCATCCCCGGCCTGAACCTC
TCCCGGGGCGATCCCTCCGT

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKQSIRAGLMLVVAALVVLAAFPAAAKVRGRVVEYRDGTVTMKGYLAWDPALKGKRPGVLVVHEWWGHNEYARKRARMLA
GLGYTALAVDMYGEGKQAPHPDDAAAFAAEVMKNGNLMKDRFMAAMNLLKDQPTVDPGRIGAIGYCFGGAVVLNMARQGV
DLAGVVSFHGSLATDRPAEPGGIKARVLVLNGAADRFVPPEQVGAFAAEMARSGAEFGFISYAGAKHSFTNPEADAFAKR
FGLDVAYDARADRRSWAEMKRFFHLCFSGG

Sequences:

>Translated_270_residues
MKQSIRAGLMLVVAALVVLAAFPAAAKVRGRVVEYRDGTVTMKGYLAWDPALKGKRPGVLVVHEWWGHNEYARKRARMLA
GLGYTALAVDMYGEGKQAPHPDDAAAFAAEVMKNGNLMKDRFMAAMNLLKDQPTVDPGRIGAIGYCFGGAVVLNMARQGV
DLAGVVSFHGSLATDRPAEPGGIKARVLVLNGAADRFVPPEQVGAFAAEMARSGAEFGFISYAGAKHSFTNPEADAFAKR
FGLDVAYDARADRRSWAEMKRFFHLCFSGG
>Mature_270_residues
MKQSIRAGLMLVVAALVVLAAFPAAAKVRGRVVEYRDGTVTMKGYLAWDPALKGKRPGVLVVHEWWGHNEYARKRARMLA
GLGYTALAVDMYGEGKQAPHPDDAAAFAAEVMKNGNLMKDRFMAAMNLLKDQPTVDPGRIGAIGYCFGGAVVLNMARQGV
DLAGVVSFHGSLATDRPAEPGGIKARVLVLNGAADRFVPPEQVGAFAAEMARSGAEFGFISYAGAKHSFTNPEADAFAKR
FGLDVAYDARADRRSWAEMKRFFHLCFSGG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17536419, Length=269, Percent_Identity=36.8029739776952, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17532655, Length=245, Percent_Identity=35.1020408163265, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17532653, Length=249, Percent_Identity=34.9397590361446, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17536415, Length=262, Percent_Identity=30.1526717557252, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193204305, Length=216, Percent_Identity=37.037037037037, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17534829, Length=163, Percent_Identity=40.4907975460123, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 29042; Mature: 29042

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSIRAGLMLVVAALVVLAAFPAAAKVRGRVVEYRDGTVTMKGYLAWDPALKGKRPGVL
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECCEEEEEEEEEECCCCCCCCCCEE
VVHEWWGHNEYARKRARMLAGLGYTALAVDMYGEGKQAPHPDDAAAFAAEVMKNGNLMKD
EEECCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
RFMAAMNLLKDQPTVDPGRIGAIGYCFGGAVVLNMARQGVDLAGVVSFHGSLATDRPAEP
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCC
GGIKARVLVLNGAADRFVPPEQVGAFAAEMARSGAEFGFISYAGAKHSFTNPEADAFAKR
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
FGLDVAYDARADRRSWAEMKRFFHLCFSGG
HCCCEEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQSIRAGLMLVVAALVVLAAFPAAAKVRGRVVEYRDGTVTMKGYLAWDPALKGKRPGVL
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECCEEEEEEEEEECCCCCCCCCCEE
VVHEWWGHNEYARKRARMLAGLGYTALAVDMYGEGKQAPHPDDAAAFAAEVMKNGNLMKD
EEECCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
RFMAAMNLLKDQPTVDPGRIGAIGYCFGGAVVLNMARQGVDLAGVVSFHGSLATDRPAEP
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCC
GGIKARVLVLNGAADRFVPPEQVGAFAAEMARSGAEFGFISYAGAKHSFTNPEADAFAKR
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
FGLDVAYDARADRRSWAEMKRFFHLCFSGG
HCCCEEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]