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The map label for this gene is nfsA [C]

Identifier: 39997620

GI number: 39997620

Start: 2783251

End: 2783754

Strand: Reverse

Name: nfsA [C]

Synonym: GSU2525

Alternate gene names: 39997620

Gene position: 2783754-2783251 (Counterclockwise)

Preceding gene: 39997621

Following gene: 39997617

Centisome position: 72.99

GC content: 67.26

Gene sequence:

>504_bases
ATGGAACTGCTCGAGGGAATTCTTTCGCGCCGCAGCGTGCGGCAATTCACCGATGAACCGGTGAGCCCCGCAGAGGTGGA
GGATATCCTCCGGGCCGGGGCATGGGCACCGTCCGGGCTCAACAATCAGCCGTGGCGGTTCGCCGTGGTCCGCGACCGGG
AAAAGCTCCGGTCCCTGGCGGGGCTCACCCGCTACCGCCACATCGTGGAAGGAGCGCCGGTCAGCATTGCGGTGTTCTGC
GACCGGGACGCCATGTACCACGAGACCAAGGACCATCAGGCCATCGGAGCCTGCCTCCAGAACATGCTGCTCGCGGCCCA
CGCCCTGGGGCTCGGGGCCGTGTGGCTGGGCGAGATACTGAAGAGCGCCGGTCAGGTGCGCGACCTGCTGGGGCTCCCTC
CGAATCTGGAGCTCATGGCGGTGATCGCCGTGGGCCGTCCGTCCGGGGTCCCCCACCACTCGGACCGCAAGCCCCTGTCA
GAGCTGATCGTCGCTGAATTCTGA

Upstream 100 bases:

>100_bases
GCCGGATAGCCTTTTCCATGTCGGTCCACCAGGCCATGAGCCTCGTTTCGGCCATGACGGTGGGAGGATCGTTCATCTAC
CTGTGGCGGTTGATGCGCTG

Downstream 100 bases:

>100_bases
CTCCGCAGTACCTGCCGCTTCCTATCCCCCGTCGCTCCCCCCCCCGTGCAATCCGTGCCGTGTCATCTTGTAGCGTAGCG
TTCCGCGCGGAATCTTGAGA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MELLEGILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDREKLRSLAGLTRYRHIVEGAPVSIAVFC
DRDAMYHETKDHQAIGACLQNMLLAAHALGLGAVWLGEILKSAGQVRDLLGLPPNLELMAVIAVGRPSGVPHHSDRKPLS
ELIVAEF

Sequences:

>Translated_167_residues
MELLEGILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDREKLRSLAGLTRYRHIVEGAPVSIAVFC
DRDAMYHETKDHQAIGACLQNMLLAAHALGLGAVWLGEILKSAGQVRDLLGLPPNLELMAVIAVGRPSGVPHHSDRKPLS
ELIVAEF
>Mature_167_residues
MELLEGILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDREKLRSLAGLTRYRHIVEGAPVSIAVFC
DRDAMYHETKDHQAIGACLQNMLLAAHALGLGAVWLGEILKSAGQVRDLLGLPPNLELMAVIAVGRPSGVPHHSDRKPLS
ELIVAEF

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 18332; Mature: 18332

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLEGILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDREKLRSLA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHH
GLTRYRHIVEGAPVSIAVFCDRDAMYHETKDHQAIGACLQNMLLAAHALGLGAVWLGEIL
HHHHHHHHHCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAGQVRDLLGLPPNLELMAVIAVGRPSGVPHHSDRKPLSELIVAEF
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MELLEGILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDREKLRSLA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHH
GLTRYRHIVEGAPVSIAVFCDRDAMYHETKDHQAIGACLQNMLLAAHALGLGAVWLGEIL
HHHHHHHHHCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAGQVRDLLGLPPNLELMAVIAVGRPSGVPHHSDRKPLSELIVAEF
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]