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The map label for this gene is kinE [H]

Identifier: 39997602

GI number: 39997602

Start: 2763705

End: 2765612

Strand: Reverse

Name: kinE [H]

Synonym: GSU2507

Alternate gene names: 39997602

Gene position: 2765612-2763705 (Counterclockwise)

Preceding gene: 39997606

Following gene: 39997601

Centisome position: 72.51

GC content: 62.11

Gene sequence:

>1908_bases
TTGTATTTAAAATGTAATGTTGTTTCGAGTCCCGCTTTGTCCATGCGCAAAATCAGCCTGAAAGCAAAAATCGTCACGAT
TGTGCTGCTGGTCTTTTTCGGCGTTGCACCGGCCACCTCCTATTTCGTCATCGGTTACTTCAAGGACCGGTTACAGGAGT
CGATCCAGAACCAGCAGGTCATTCTGGTGAGCCGGACCGCGTCGGAGCTGGATTTCAAGATATCCTCCGCCCAGAGGATG
CTTGTATCCGTGGCCCTGAGCATCCCCGAACAGGTCGTCCGCGACCCGCGGGCAGCGGAGCGTTTCCTCTACGAACGCTC
GTTCCTCAGCATGTATTTCGACAGCGGCACCTTCCTGCTGAACCCCGGGGGCCAGTTGATCGCCGAGACCGCCTGGAAGC
CGAGCCGCGTCGGCCGTGACTATTCGTTCAGGGAATACGTCAAGGATACCCTCGCCACCGGCAAGCCGGTCATTTCCGCT
CCCTATGTCCCCCGGACCCTGCACCATCCCGTTGTAATGTTCACCGCGCCGGTGTTCGGCTCCGGGGGCAAGCTGATCGC
CATCCTGGCCGGCAGCACCGATCTCTATAAGGATAACCTCCTGGGCGATCTCTCCACCGCCCGGATCGGCTCGCTGGGAT
ACTTCAGCCTCTACGACCACGAAGGGGTGGTTATCATGCATCCGGTGCGTGAGCGGATTTTCAGCCCCGCGGACAAGCAG
GCCGAGGGTATGACGGCGCGCATCGCCCAGGCGAGACGCGGCGCCAATGTCCAGGGCGAGCTCGGACAGCGGGACGGCAG
CCGGACCCTCTCAACCATGAAGAAGCTGGGCAGCATCGACTGGATCCTGGCCGCTTCATATCCGGTGGAAGAGGCCTACG
CGCCCGTGCAGCGCCTGGTCCGGCTCTCCTGGTGGATCATGGGCTGCGGCGGCGTCTTTGCCGCCCTGCTCATGTGGATG
ACCATGAGCCATCTCATCTCGCCGCTGCTCTCCCTCACCCGCCAGATCAGGCAGCTGGGGGCGTCAAACGGCGACCTGCC
CATGGTCCGGATCGACTCCAACGACGAAATCCGCGAAACCGCCGAGGCGATCAACGGCCTGCTGGAGCGGCTGCGGCTCA
AGGAGATCGAAAAGGAGACGATCCAGAAGGACGCCATGCAGACCTGGCACCTGGCGTCCCTGGGCGAAGTCGCGGCCGGC
GTGGCCCACGAGATCAACAACCCCATCAACGGCATCATCAACTGTGCCCAGATCCTGGCGGCGGAGCCGTGCCTCAGCGA
GCCGGAACGGGGGGTTGCGAGCAGGATCATCAAGGAGGGGGAGCGGGTGGCGGTCATCGTCCGGAGCCTGCTCTCCTTTG
CCCGCTACGAGGGGAGGGAGCGGGTCCCCGCCTATGTGCCCGACATCCTCAACGACACCCTCGAACTGACCAGGGTCCAG
CTGAAGCGTGACGGGGTCCAGCTCCACGTGGATCTGCCGTCGGACCTGCCGGCGCTGCTGGTGCACCCGCACCAGATCGA
GCAGGTGTTCCTCAACCTCATCAGCAACGCGCGCTACGCCCTGGACAAGAAGTTCTCCGCCGGCGCGGGAGGGGGCAAGC
GCCTGGATATCAAGGGAGAAAAGATTATCGGAGATGACGGCGTCTTTGTCCGGATCGAGTTCAGGGACAACGGCACCGGC
ATCCCGCGCGATGTGATCGGGAGGGTCATCGACCCCTTCTTCACGACCAAGCCTGCCGCTGAAGGCACGGGCCTGGGGCT
GAGCATCAGTCACGGCCTGATCTCGGACCATGGAGGGCGACTGAAGGTTGAAAGCGTCGAGGGAGAGTTCACCCGTGTAA
TGATTGATTTGCCCGTAATGACCTGGGAGGACGAGTATGGCCAGAAGGGTTCTGATAGTAGAGGATGA

Upstream 100 bases:

>100_bases
TTTGTTAATCACGGGGCTCTGCACGGCGCAACTGGTTGTTAGCTGATTATCATCATTTGGGGTGTGACACACATAACTGT
ATTGTTATAAATTGCATATA

Downstream 100 bases:

>100_bases
GGACACGCTGCGGTTCACGTTCGAGCACTTCCTCGCGGCCGAGGGCTATGCCGTCGATACGGCGGCCGATTTCGCGGAGG
CGATCGGCAAGGTCGAATCC

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MYLKCNVVSSPALSMRKISLKAKIVTIVLLVFFGVAPATSYFVIGYFKDRLQESIQNQQVILVSRTASELDFKISSAQRM
LVSVALSIPEQVVRDPRAAERFLYERSFLSMYFDSGTFLLNPGGQLIAETAWKPSRVGRDYSFREYVKDTLATGKPVISA
PYVPRTLHHPVVMFTAPVFGSGGKLIAILAGSTDLYKDNLLGDLSTARIGSLGYFSLYDHEGVVIMHPVRERIFSPADKQ
AEGMTARIAQARRGANVQGELGQRDGSRTLSTMKKLGSIDWILAASYPVEEAYAPVQRLVRLSWWIMGCGGVFAALLMWM
TMSHLISPLLSLTRQIRQLGASNGDLPMVRIDSNDEIRETAEAINGLLERLRLKEIEKETIQKDAMQTWHLASLGEVAAG
VAHEINNPINGIINCAQILAAEPCLSEPERGVASRIIKEGERVAVIVRSLLSFARYEGRERVPAYVPDILNDTLELTRVQ
LKRDGVQLHVDLPSDLPALLVHPHQIEQVFLNLISNARYALDKKFSAGAGGGKRLDIKGEKIIGDDGVFVRIEFRDNGTG
IPRDVIGRVIDPFFTTKPAAEGTGLGLSISHGLISDHGGRLKVESVEGEFTRVMIDLPVMTWEDEYGQKGSDSRG

Sequences:

>Translated_635_residues
MYLKCNVVSSPALSMRKISLKAKIVTIVLLVFFGVAPATSYFVIGYFKDRLQESIQNQQVILVSRTASELDFKISSAQRM
LVSVALSIPEQVVRDPRAAERFLYERSFLSMYFDSGTFLLNPGGQLIAETAWKPSRVGRDYSFREYVKDTLATGKPVISA
PYVPRTLHHPVVMFTAPVFGSGGKLIAILAGSTDLYKDNLLGDLSTARIGSLGYFSLYDHEGVVIMHPVRERIFSPADKQ
AEGMTARIAQARRGANVQGELGQRDGSRTLSTMKKLGSIDWILAASYPVEEAYAPVQRLVRLSWWIMGCGGVFAALLMWM
TMSHLISPLLSLTRQIRQLGASNGDLPMVRIDSNDEIRETAEAINGLLERLRLKEIEKETIQKDAMQTWHLASLGEVAAG
VAHEINNPINGIINCAQILAAEPCLSEPERGVASRIIKEGERVAVIVRSLLSFARYEGRERVPAYVPDILNDTLELTRVQ
LKRDGVQLHVDLPSDLPALLVHPHQIEQVFLNLISNARYALDKKFSAGAGGGKRLDIKGEKIIGDDGVFVRIEFRDNGTG
IPRDVIGRVIDPFFTTKPAAEGTGLGLSISHGLISDHGGRLKVESVEGEFTRVMIDLPVMTWEDEYGQKGSDSRG
>Mature_635_residues
MYLKCNVVSSPALSMRKISLKAKIVTIVLLVFFGVAPATSYFVIGYFKDRLQESIQNQQVILVSRTASELDFKISSAQRM
LVSVALSIPEQVVRDPRAAERFLYERSFLSMYFDSGTFLLNPGGQLIAETAWKPSRVGRDYSFREYVKDTLATGKPVISA
PYVPRTLHHPVVMFTAPVFGSGGKLIAILAGSTDLYKDNLLGDLSTARIGSLGYFSLYDHEGVVIMHPVRERIFSPADKQ
AEGMTARIAQARRGANVQGELGQRDGSRTLSTMKKLGSIDWILAASYPVEEAYAPVQRLVRLSWWIMGCGGVFAALLMWM
TMSHLISPLLSLTRQIRQLGASNGDLPMVRIDSNDEIRETAEAINGLLERLRLKEIEKETIQKDAMQTWHLASLGEVAAG
VAHEINNPINGIINCAQILAAEPCLSEPERGVASRIIKEGERVAVIVRSLLSFARYEGRERVPAYVPDILNDTLELTRVQ
LKRDGVQLHVDLPSDLPALLVHPHQIEQVFLNLISNARYALDKKFSAGAGGGKRLDIKGEKIIGDDGVFVRIEFRDNGTG
IPRDVIGRVIDPFFTTKPAAEGTGLGLSISHGLISDHGGRLKVESVEGEFTRVMIDLPVMTWEDEYGQKGSDSRG

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=253, Percent_Identity=30.4347826086957, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=34.412955465587, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=30.6772908366534, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789149, Length=263, Percent_Identity=25.4752851711027, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=29.4776119402985, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=272, Percent_Identity=27.5735294117647, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=24.8868778280543, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70051; Mature: 70051

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKCNVVSSPALSMRKISLKAKIVTIVLLVFFGVAPATSYFVIGYFKDRLQESIQNQQV
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCEE
ILVSRTASELDFKISSAQRMLVSVALSIPEQVVRDPRAAERFLYERSFLSMYFDSGTFLL
EEEECCCCHHCEEHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
NPGGQLIAETAWKPSRVGRDYSFREYVKDTLATGKPVISAPYVPRTLHHPVVMFTAPVFG
CCCCHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCHHCCCEEEEECCEEC
SGGKLIAILAGSTDLYKDNLLGDLSTARIGSLGYFSLYDHEGVVIMHPVRERIFSPADKQ
CCCCEEEEEECCCHHHHHHHCCCCHHHHHCCCCEEEEECCCCEEEECCHHHHHCCCCCCH
AEGMTARIAQARRGANVQGELGQRDGSRTLSTMKKLGSIDWILAASYPVEEAYAPVQRLV
HCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
RLSWWIMGCGGVFAALLMWMTMSHLISPLLSLTRQIRQLGASNGDLPMVRIDSNDEIRET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
AEAINGLLERLRLKEIEKETIQKDAMQTWHLASLGEVAAGVAHEINNPINGIINCAQILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEPCLSEPERGVASRIIKEGERVAVIVRSLLSFARYEGRERVPAYVPDILNDTLELTRVQ
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LKRDGVQLHVDLPSDLPALLVHPHQIEQVFLNLISNARYALDKKFSAGAGGGKRLDIKGE
HCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCC
KIIGDDGVFVRIEFRDNGTGIPRDVIGRVIDPFFTTKPAAEGTGLGLSISHGLISDHGGR
EEECCCCEEEEEEEECCCCCCCHHHHHHHHCHHHCCCCCCCCCCCCEEECCCCCCCCCCE
LKVESVEGEFTRVMIDLPVMTWEDEYGQKGSDSRG
EEEEECCCCEEEEEEEECEEECCHHHCCCCCCCCC
>Mature Secondary Structure
MYLKCNVVSSPALSMRKISLKAKIVTIVLLVFFGVAPATSYFVIGYFKDRLQESIQNQQV
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCEE
ILVSRTASELDFKISSAQRMLVSVALSIPEQVVRDPRAAERFLYERSFLSMYFDSGTFLL
EEEECCCCHHCEEHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
NPGGQLIAETAWKPSRVGRDYSFREYVKDTLATGKPVISAPYVPRTLHHPVVMFTAPVFG
CCCCHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCHHCCCEEEEECCEEC
SGGKLIAILAGSTDLYKDNLLGDLSTARIGSLGYFSLYDHEGVVIMHPVRERIFSPADKQ
CCCCEEEEEECCCHHHHHHHCCCCHHHHHCCCCEEEEECCCCEEEECCHHHHHCCCCCCH
AEGMTARIAQARRGANVQGELGQRDGSRTLSTMKKLGSIDWILAASYPVEEAYAPVQRLV
HCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
RLSWWIMGCGGVFAALLMWMTMSHLISPLLSLTRQIRQLGASNGDLPMVRIDSNDEIRET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
AEAINGLLERLRLKEIEKETIQKDAMQTWHLASLGEVAAGVAHEINNPINGIINCAQILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEPCLSEPERGVASRIIKEGERVAVIVRSLLSFARYEGRERVPAYVPDILNDTLELTRVQ
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LKRDGVQLHVDLPSDLPALLVHPHQIEQVFLNLISNARYALDKKFSAGAGGGKRLDIKGE
HCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCC
KIIGDDGVFVRIEFRDNGTGIPRDVIGRVIDPFFTTKPAAEGTGLGLSISHGLISDHGGR
EEECCCCEEEEEEEECCCCCCCHHHHHHHHCHHHCCCCCCCCCCCCEEECCCCCCCCCCE
LKVESVEGEFTRVMIDLPVMTWEDEYGQKGSDSRG
EEEEECCCCEEEEEEEECEEECCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]