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The map label for this gene is trpB2 [H]

Identifier: 39997474

GI number: 39997474

Start: 2607448

End: 2608803

Strand: Reverse

Name: trpB2 [H]

Synonym: GSU2379

Alternate gene names: 39997474

Gene position: 2608803-2607448 (Counterclockwise)

Preceding gene: 39997475

Following gene: 39997473

Centisome position: 68.4

GC content: 66.45

Gene sequence:

>1356_bases
ATGCAAACTAAAATAGTGCTGGACGAAAGCCGCATCCCGACCCACTGGTACAACATCATCCCCGACATGCCCGGCCCCCT
GGCGCCGGTCATCAACCCCCGCACCCTTCAGCCGGTGACCCCCGACGACCTTCTCCCCATCTTCCCCATGGCCATCATCG
AGCAGGAGATTTCGGGCGAACGGTGGATTCCGATCCCGGAAGAGGTGCGGGAGATCTACCGCCTCTGGCGGCCGTCTCCC
CTCTACCGTGCCCACCGGCTGGAGCAGGCCCTCGGCACCCCGGCGAAGATCTACTACAAATACGAAGGGGTGTCCCCGGC
CGGCTCACACAAGCCCAACTCCGCCATCCCCCAGGCGTACTACAACAAGCAGGCGGGCATCCGGCGGCTGGCCACCGAGA
CCGGCGCCGGGCAGTGGGGGAGTTCCCTGGCCCTGGCCTGCTCCATGTTCGGCCTGGAGTGCACGGTCTACATGGTCAAG
GTGTCGTGCACCCAGAAGCCGTACCGCAAAAGCATGATGCAGCTCTGGGGGGCCAACGTGATCCCGTCGCCGTCCGAGTT
CACCAACGCCGGGCGCTCCATCCTGGCCCACGATCCGGACAGCAACGGCAGCCTCGGCATCGCCATCTCCGAGGCGGTTG
AGGATGCCGCCTCCCGCGCCGACACCAACTATGCCCTGGGCAGCGTTCTGAACCACGTCTGCCTCCACCAGACCATCATC
GGCCAGGAGGCCAGGGAACAGCTGGCCCTGGCCGGCGACTACCCCGACGTGGTCATCGCCTGCTGCGGCGGCGGCTCCAA
CTTCGCCGGCACGGCCTTCCCGTTCCTGGCCGACCGGGCCGCCGGCAAGAACGTCCGCTGTCTGGCCGTGGAACCCGCCT
CGTGCCCGACCCTGACCAAGGGGATCTACGCCTTCGACTACGGCGACACCGCCAAGATGGCCCCCATCGCCATGATGTAC
ACCCTGGGGCACGACTTCATGCCGCCCGGTATCCACGCCGGCGGGCTGCGCTACCACGGCGAGTCGCCCCTGGTGTCGCA
ACTCCACCATGCCGGCCTCATCGAGGCCAAGTCGTACCGCCAGACCGCCTGTTTCGAGGCAGCCCACCTCTTTGCCCGGC
ACGAGGGGATCGTGCCGGCCCCCGAATCGTCCCACGCGGTGCGGGCCGCCATTGACGAAGCAGTCATCGCCAAGGAGGAG
GGGAAGGAAAAGACCATTCTCTTCTGCCTCTCGGGCCACGGCCAGCTGGACATGGTGGCCTACGACGCCTACCTCTCCGG
CGGCCTGGAAGACGTCGAATACCCCGAGGAGATGATCCGGGAGTCCCTGGCCAAACTGCCCAAGGTGGAACTGTGA

Upstream 100 bases:

>100_bases
CCCTCATGCGGGAAGAGGATATCGGGGCAAAACTGCGGGAGTTGCTGGGATAGTTCAGTGGCCCCCACGATCAGACAGAC
GCCATCGCAAAGGAGATCCG

Downstream 100 bases:

>100_bases
ACGGATGGTCCGCGTCAAGATCTGCGGCATAACATCCCTTGAGGATGCCCTCGTGGCGGTGGAGGCCGGGGCCGACGCCC
TCGGCTTCGTCTTTCACGAC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQTKIVLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEISGERWIPIPEEVREIYRLWRPSP
LYRAHRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRSILAHDPDSNGSLGIAISEAVEDAASRADTNYALGSVLNHVCLHQTII
GQEAREQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGIYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLHHAGLIEAKSYRQTACFEAAHLFARHEGIVPAPESSHAVRAAIDEAVIAKEE
GKEKTILFCLSGHGQLDMVAYDAYLSGGLEDVEYPEEMIRESLAKLPKVEL

Sequences:

>Translated_451_residues
MQTKIVLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEISGERWIPIPEEVREIYRLWRPSP
LYRAHRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRSILAHDPDSNGSLGIAISEAVEDAASRADTNYALGSVLNHVCLHQTII
GQEAREQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGIYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLHHAGLIEAKSYRQTACFEAAHLFARHEGIVPAPESSHAVRAAIDEAVIAKEE
GKEKTILFCLSGHGQLDMVAYDAYLSGGLEDVEYPEEMIRESLAKLPKVEL
>Mature_451_residues
MQTKIVLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEISGERWIPIPEEVREIYRLWRPSP
LYRAHRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRSILAHDPDSNGSLGIAISEAVEDAASRADTNYALGSVLNHVCLHQTII
GQEAREQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGIYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLHHAGLIEAKSYRQTACFEAAHLFARHEGIVPAPESSHAVRAAIDEAVIAKEE
GKEKTILFCLSGHGQLDMVAYDAYLSGGLEDVEYPEEMIRESLAKLPKVEL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=359, Percent_Identity=30.3621169916435, Blast_Score=121, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=366, Percent_Identity=31.4207650273224, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49123; Mature: 49123

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKIVLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEISGE
CCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RWIPIPEEVREIYRLWRPSPLYRAHRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAY
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVKVSCTQKPYRKSMMQLWGANV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRSILAHDPDSNGSLGIAISEAVEDAASRADTNYALGSVLNHVCLHQTII
CCCCHHHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GQEAREQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHC
GIYAFDYGDTAKMAPIAMMYTLGHDFMPPGIHAGGLRYHGESPLVSQLHHAGLIEAKSYR
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHH
QTACFEAAHLFARHEGIVPAPESSHAVRAAIDEAVIAKEEGKEKTILFCLSGHGQLDMVA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
YDAYLSGGLEDVEYPEEMIRESLAKLPKVEL
EHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTKIVLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEISGE
CCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RWIPIPEEVREIYRLWRPSPLYRAHRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAY
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVKVSCTQKPYRKSMMQLWGANV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRSILAHDPDSNGSLGIAISEAVEDAASRADTNYALGSVLNHVCLHQTII
CCCCHHHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GQEAREQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHC
GIYAFDYGDTAKMAPIAMMYTLGHDFMPPGIHAGGLRYHGESPLVSQLHHAGLIEAKSYR
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHH
QTACFEAAHLFARHEGIVPAPESSHAVRAAIDEAVIAKEEGKEKTILFCLSGHGQLDMVA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
YDAYLSGGLEDVEYPEEMIRESLAKLPKVEL
EHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]