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The map label for this gene is gcvH-2 [H]

Identifier: 39997116

GI number: 39997116

Start: 2211797

End: 2212195

Strand: Reverse

Name: gcvH-2 [H]

Synonym: GSU2018

Alternate gene names: 39997116

Gene position: 2212195-2211797 (Counterclockwise)

Preceding gene: 39997117

Following gene: 39997115

Centisome position: 58.0

GC content: 56.64

Gene sequence:

>399_bases
ATGGATTTTCCTGAAGAGCTCAAGTACAGCAAGGAACACGTTTGGGTCAGGGAAGAGGGGGATCGGGCGGTCATCGGGCT
GACCGATTATGCCCAGGATGCCTTGGGCACCATCAGTGCCATAGAACTGCCCACAGTTGGGGATGAACTGGAGCAGGAGG
ATTCCTTCGGCTCCCTTGAGGCACGCAAGACTGCGTCGGAACTTTACGCTCCCATCAGCGGGACGGTGCTGGAGGTAAAC
GAGGAATTGGACGCGGCTCCGGAGGTCATCAATGACGACCCCTATGACGGGGGCTGGATTGCGGCGGTCTCCCTTGATGA
CCCTGAAGAACTCAAGTCGCTCCTGTCGGCGGAAGATTACGCAGAGTATGTAAGCCAGACCGACGAAGACGAAGAATAA

Upstream 100 bases:

>100_bases
GACCACAATCCCCTTCCATAAGAGGATCATGGACAACAAAGACTTTATGGAGGGGAATGTCGACACCGGCTTCCTCGAGC
GAATCGTGCTGGAGTAGGGC

Downstream 100 bases:

>100_bases
CCAACAGCGGAGTTTCAGCCCGCTGACAAAGGCGAGATACCCATCTCGCCTTTGTTTTTGACCATAACCGGAGCACGCAA
TGCCACTGACGCTCGGCCAT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MDFPEELKYSKEHVWVREEGDRAVIGLTDYAQDALGTISAIELPTVGDELEQEDSFGSLEARKTASELYAPISGTVLEVN
EELDAAPEVINDDPYDGGWIAAVSLDDPEELKSLLSAEDYAEYVSQTDEDEE

Sequences:

>Translated_132_residues
MDFPEELKYSKEHVWVREEGDRAVIGLTDYAQDALGTISAIELPTVGDELEQEDSFGSLEARKTASELYAPISGTVLEVN
EELDAAPEVINDDPYDGGWIAAVSLDDPEELKSLLSAEDYAEYVSQTDEDEE
>Mature_132_residues
MDFPEELKYSKEHVWVREEGDRAVIGLTDYAQDALGTISAIELPTVGDELEQEDSFGSLEARKTASELYAPISGTVLEVN
EELDAAPEVINDDPYDGGWIAAVSLDDPEELKSLLSAEDYAEYVSQTDEDEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=40.9836065573771, Blast_Score=111, Evalue=2e-25,
Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=40.1639344262295, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=44.1666666666667, Blast_Score=107, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=38.9830508474576, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=40.8695652173913, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=40.495867768595, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14576; Mature: 14576

Theoretical pI: Translated: 3.49; Mature: 3.49

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPEELKYSKEHVWVREEGDRAVIGLTDYAQDALGTISAIELPTVGDELEQEDSFGSLE
CCCCHHHCCCCCEEEEEECCCEEEEEEHHHHHHHHHCHHEEECCCCCCHHHCCCCCCCCH
ARKTASELYAPISGTVLEVNEELDAAPEVINDDPYDGGWIAAVSLDDPEELKSLLSAEDY
HHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHCHHHH
AEYVSQTDEDEE
HHHHHCCCCCCC
>Mature Secondary Structure
MDFPEELKYSKEHVWVREEGDRAVIGLTDYAQDALGTISAIELPTVGDELEQEDSFGSLE
CCCCHHHCCCCCEEEEEECCCEEEEEEHHHHHHHHHCHHEEECCCCCCHHHCCCCCCCCH
ARKTASELYAPISGTVLEVNEELDAAPEVINDDPYDGGWIAAVSLDDPEELKSLLSAEDY
HHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHCHHHH
AEYVSQTDEDEE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA