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The map label for this gene is miaA

Identifier: 39997098

GI number: 39997098

Start: 2191725

End: 2192660

Strand: Reverse

Name: miaA

Synonym: GSU2000

Alternate gene names: 39997098

Gene position: 2192660-2191725 (Counterclockwise)

Preceding gene: 39997099

Following gene: 39997097

Centisome position: 57.49

GC content: 60.26

Gene sequence:

>936_bases
ATGGGCAATCAGGCCGGGTCAAAGACAAAACTCGTCATAATCCAGGGGCCGACTGCATCGGGCAAGTCAGAACTTGCGGT
CCGGCTGGCGGAGGCCTGCGGCGGGGACGTGGTGAATGCCGACTCCATGCAGGTCTACCGGGGAATGGACATCGGCACGG
CAAAGCCGTCCCCGGAGCTCGTGGCCCGGGTTCCCCACCACCTCTACGACATCGTCGACCCGGACGTGAACTTCACCGCA
GCCGACTATCGCAGAGAGGCGGGGCGGGTCATCACCGAGATCCACAGCCGGGGAAAGCGCCCCATTCTCGTGGGTGGGAC
GGGACTTTACATCAAGACCCTTATTGGTGGGCTCGCCCCATCGCCCGGCGCTGACGATACGATCCGGGCCGAGTTGGAGG
AAGATGCCCGGAGCGAAGGGGCGGCTGCGCTCCACGACCGGCTGGCACAGGTGGATCCGGCCGCGGCGGCGCGGCTCCAC
CCCAATGATCGGGTCCGGATCGTCAGGGCGCTGGAGGTGTTCCTAATGACCGGGAAGCCTCTTTCGGAGTTCCAGGAGGA
GCACCGTTTCGCCGATGAGCCTTATGATTGCCTGAAATTAGGCATTTCCGTGGAACGTGACGATCTTTACCGGCGCATCA
ACGAGCGGGTGAACCGGATGTTCGCTGAAGGCTTTGTCGAGGAGGTGAGCGGTCTGTTGAATGCGGGCTATTCGCCTGAC
CTCAAGGCCATGGGGGCCATTGGTTACAAGGAAGTCTGCGCGTATCTGGCCGGAACCTGTTCGCTGGACGAGGCGCGGGA
ACTCGTTCAACGGAACACCCGCCGCTATGCAAAGAGGCAATTGACGTGGTTTCGCAAGGATCCTGCAATAAAGTGGGTTG
AATATCCGGCCAATTTTGATAGTATCTCTACCATTGCGATGAAATTTTTTGGATGA

Upstream 100 bases:

>100_bases
CCACCGACTTTTCCACCAATTGCCCCCATGGCAGGCCCGTCCTCCAGCGACTTACGCTGGGGGAGGTGGAGAAGATGTTC
AAGAGAGTGTGACCGCTATC

Downstream 100 bases:

>100_bases
AGGAGCGGCACTATGGCGAAAGCACCATTCAACATTCAGGACCAGTACCTCAACCAGTCGCGTAAGGAGCGGATCAAGGT
CGCCGTGCGGCTCATGTCGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MGNQAGSKTKLVIIQGPTASGKSELAVRLAEACGGDVVNADSMQVYRGMDIGTAKPSPELVARVPHHLYDIVDPDVNFTA
ADYRREAGRVITEIHSRGKRPILVGGTGLYIKTLIGGLAPSPGADDTIRAELEEDARSEGAAALHDRLAQVDPAAAARLH
PNDRVRIVRALEVFLMTGKPLSEFQEEHRFADEPYDCLKLGISVERDDLYRRINERVNRMFAEGFVEEVSGLLNAGYSPD
LKAMGAIGYKEVCAYLAGTCSLDEARELVQRNTRRYAKRQLTWFRKDPAIKWVEYPANFDSISTIAMKFFG

Sequences:

>Translated_311_residues
MGNQAGSKTKLVIIQGPTASGKSELAVRLAEACGGDVVNADSMQVYRGMDIGTAKPSPELVARVPHHLYDIVDPDVNFTA
ADYRREAGRVITEIHSRGKRPILVGGTGLYIKTLIGGLAPSPGADDTIRAELEEDARSEGAAALHDRLAQVDPAAAARLH
PNDRVRIVRALEVFLMTGKPLSEFQEEHRFADEPYDCLKLGISVERDDLYRRINERVNRMFAEGFVEEVSGLLNAGYSPD
LKAMGAIGYKEVCAYLAGTCSLDEARELVQRNTRRYAKRQLTWFRKDPAIKWVEYPANFDSISTIAMKFFG
>Mature_310_residues
GNQAGSKTKLVIIQGPTASGKSELAVRLAEACGGDVVNADSMQVYRGMDIGTAKPSPELVARVPHHLYDIVDPDVNFTAA
DYRREAGRVITEIHSRGKRPILVGGTGLYIKTLIGGLAPSPGADDTIRAELEEDARSEGAAALHDRLAQVDPAAAARLHP
NDRVRIVRALEVFLMTGKPLSEFQEEHRFADEPYDCLKLGISVERDDLYRRINERVNRMFAEGFVEEVSGLLNAGYSPDL
KAMGAIGYKEVCAYLAGTCSLDEARELVQRNTRRYAKRQLTWFRKDPAIKWVEYPANFDSISTIAMKFFG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=35.6687898089172, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=42.5170068027211, Blast_Score=231, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=28.9036544850498, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=32.1799307958478, Blast_Score=165, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=31.1377245508982, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_GEOSL (Q74BP1)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953049.1
- ProteinModelPortal:   Q74BP1
- SMR:   Q74BP1
- GeneID:   2686109
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2000
- NMPDR:   fig|243231.1.peg.1987
- TIGR:   GSU2000
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   GSUL243231:GSU_2000-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34299; Mature: 34168

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQAGSKTKLVIIQGPTASGKSELAVRLAEACGGDVVNADSMQVYRGMDIGTAKPSPEL
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHH
VARVPHHLYDIVDPDVNFTAADYRREAGRVITEIHSRGKRPILVGGTGLYIKTLIGGLAP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCC
SPGADDTIRAELEEDARSEGAAALHDRLAQVDPAAAARLHPNDRVRIVRALEVFLMTGKP
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCC
LSEFQEEHRFADEPYDCLKLGISVERDDLYRRINERVNRMFAEGFVEEVSGLLNAGYSPD
HHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LKAMGAIGYKEVCAYLAGTCSLDEARELVQRNTRRYAKRQLTWFRKDPAIKWVEYPANFD
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCH
SISTIAMKFFG
HHHHHHHHHCC
>Mature Secondary Structure 
GNQAGSKTKLVIIQGPTASGKSELAVRLAEACGGDVVNADSMQVYRGMDIGTAKPSPEL
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHH
VARVPHHLYDIVDPDVNFTAADYRREAGRVITEIHSRGKRPILVGGTGLYIKTLIGGLAP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCC
SPGADDTIRAELEEDARSEGAAALHDRLAQVDPAAAARLHPNDRVRIVRALEVFLMTGKP
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCC
LSEFQEEHRFADEPYDCLKLGISVERDDLYRRINERVNRMFAEGFVEEVSGLLNAGYSPD
HHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LKAMGAIGYKEVCAYLAGTCSLDEARELVQRNTRRYAKRQLTWFRKDPAIKWVEYPANFD
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCH
SISTIAMKFFG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA