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The map label for this gene is asnB [H]

Identifier: 39997051

GI number: 39997051

Start: 2140879

End: 2142714

Strand: Reverse

Name: asnB [H]

Synonym: GSU1953

Alternate gene names: 39997051

Gene position: 2142714-2140879 (Counterclockwise)

Preceding gene: 39997052

Following gene: 39997050

Centisome position: 56.18

GC content: 50.6

Gene sequence:

>1836_bases
ATGTGCGGAATTGTAGGTAAATATTATTTTAATAAAAATGCCGGTGACAGCAGTGATATAGATGCAATGATGCAGGCAAT
TTACCATCGGGGTCCTGATTCCTCGGGGAAATACATAAATGGGAGGGCTGCGCTCGGGTTTCAGAGACTGAGTATTATTG
ATACGGTTTCAGGCCATCAGCCACTTTATAATGAAACAAAAACCATCGTCCTGCTTGCCAATGGGGAGATATACAATTAC
AAGGAATTCGTTCCGTTTCTGGAAAGCAAGGGCCACGTGTTTTCCACGCGGAGTGATTGTGAAGTAATTATTCACCTGTA
CGAAGAATACGGGATCGAGTTCATAAGCAAGCTCAACGGTATGTTTGCATTCTGTCTCTACGACACACGCAACGACGTCA
TGTTCATTGCGAGAGACAGGGTCGGCATCAAGCCCCTCTACTATCATCTCAACAAGGAAGCCATTATTTTCGGTTCGGAG
ATAAAGGGTATTCTGGCGTCTGGCGAGGTTGTAACCGAGGAGGCTAATGACTGCCTGGCGGAATACCTTTGTTTCAGGTA
TGTTGCAAATTTCAGGACGTTCTTCTCTTCCGTCAACGCCCTTGAGCCGGGCACGTATATCAAAATTTCTCCTAACGGTT
GTACGTTCGAAAAATTCTGGGATTTATCCAAGTACGTGTTGAACTATGCTCAAGACGGAGATCTCGTCGAGACGGTTGAT
GCCGCGTTGACAGCATCGGTGCAGCGCCAGATGATGACGGACGTTCCCCTTGGCACTCAACTAAGCGGGGGGGTCGATTC
GAGTCTTGTCAGCAAACTGGCTGCTGGATACTCACCCGGACTGAAGACCTTTACGGTCAGTTTTTTCGAAAGTGCCTATG
ACGAGTCAGCCTATGCACGGCTGCTGGCGGATTCGGCGGGGCTTGAGTATCACCAGATCAGAGTCGACGGCAAGACCTTT
GCCGACACTCTGCCCAAGGTTATCTGGTATCACGACGAGCCCCTCTGTCATGCCAACTCCGTGCATATGTACCTGCTGTG
CACGTATGCACGACAGTTTGTGACGGTCCTGCTGACCGGCGAAGGGGCCGATGAACTTTTTGCCGGTTATCCCCGTTATC
AGATTTGCAGGTTCGGCGATGCATATAACAAGTTGAATCCGAGTGTTGCTTCGCTGGTGAAGTCGGTCCTGAAGGGCGTT
CCTGCACGAAAGATCGTCAAAATAGTCGACAACCTCGGTCTTGGTGCCCGTGAACTGGCCTTGTGGAATTCATCCTTTAC
CACTAAGGAGAAGGTTTCCTGGCTGCTGGATTCCGGCGATGTCACTCTCGATGCCCGATCCGCCCTGGTGGACAGCGTCT
GGAACGATGAGTTGAGTCTTTTCGACAATCTGCTCCTCTATGAACAGAAGTCCTATCTTCAGCCGATTCTGATGCGGCAG
GACAAGATGAGCATGGCCGCCTCAGTTGAGTCCAGGGTCCCGGTCCTTGACAACGAGATGCTGGACGTGGCCAACACAAT
TCCGTATCAGTACAAAGTCCGGCACTTCACTCCCAAGCACGTGTTCAAGAAGGTTGCGGAAAGGCATATATCCCGAAAGA
TCGTCTACAAGAAAAAGGTCGGCTTCGGTGTGCCCGTGGACGAATGGCTGCGTGACCATAGCGGTTTGGGCAGGTACCTT
GATCTTCTCCTCGATACCGCCCGTGGCATCAACGGCGTCAGCAGACCGCGGATTGAGCAGTTGATTGCGGAGCATGCGAG
CCGAAGATATAACCATGGCGATGTGTTATGGCCCCTGGTCAATTATGCAATCTGGCGGGAACAATTTTTCAAGTAA

Upstream 100 bases:

>100_bases
TCTATGTTCAATAAAATTGAAAGTAACTTTGTTAAATTTTAATTTAAATTTGTGGTTATGACGACAAGAAATATAACATG
AATGATTCAAGGAAACTATA

Downstream 100 bases:

>100_bases
GGAATTATGAGCTGCGGGGCCACTGAATGAAAAAAGCTGTTTGGATTACCTGGGAACATCAGATACGCAACAAATCGATG
GCTGCCATGCTTGGTGCGGA

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MCGIVGKYYFNKNAGDSSDIDAMMQAIYHRGPDSSGKYINGRAALGFQRLSIIDTVSGHQPLYNETKTIVLLANGEIYNY
KEFVPFLESKGHVFSTRSDCEVIIHLYEEYGIEFISKLNGMFAFCLYDTRNDVMFIARDRVGIKPLYYHLNKEAIIFGSE
IKGILASGEVVTEEANDCLAEYLCFRYVANFRTFFSSVNALEPGTYIKISPNGCTFEKFWDLSKYVLNYAQDGDLVETVD
AALTASVQRQMMTDVPLGTQLSGGVDSSLVSKLAAGYSPGLKTFTVSFFESAYDESAYARLLADSAGLEYHQIRVDGKTF
ADTLPKVIWYHDEPLCHANSVHMYLLCTYARQFVTVLLTGEGADELFAGYPRYQICRFGDAYNKLNPSVASLVKSVLKGV
PARKIVKIVDNLGLGARELALWNSSFTTKEKVSWLLDSGDVTLDARSALVDSVWNDELSLFDNLLLYEQKSYLQPILMRQ
DKMSMAASVESRVPVLDNEMLDVANTIPYQYKVRHFTPKHVFKKVAERHISRKIVYKKKVGFGVPVDEWLRDHSGLGRYL
DLLLDTARGINGVSRPRIEQLIAEHASRRYNHGDVLWPLVNYAIWREQFFK

Sequences:

>Translated_611_residues
MCGIVGKYYFNKNAGDSSDIDAMMQAIYHRGPDSSGKYINGRAALGFQRLSIIDTVSGHQPLYNETKTIVLLANGEIYNY
KEFVPFLESKGHVFSTRSDCEVIIHLYEEYGIEFISKLNGMFAFCLYDTRNDVMFIARDRVGIKPLYYHLNKEAIIFGSE
IKGILASGEVVTEEANDCLAEYLCFRYVANFRTFFSSVNALEPGTYIKISPNGCTFEKFWDLSKYVLNYAQDGDLVETVD
AALTASVQRQMMTDVPLGTQLSGGVDSSLVSKLAAGYSPGLKTFTVSFFESAYDESAYARLLADSAGLEYHQIRVDGKTF
ADTLPKVIWYHDEPLCHANSVHMYLLCTYARQFVTVLLTGEGADELFAGYPRYQICRFGDAYNKLNPSVASLVKSVLKGV
PARKIVKIVDNLGLGARELALWNSSFTTKEKVSWLLDSGDVTLDARSALVDSVWNDELSLFDNLLLYEQKSYLQPILMRQ
DKMSMAASVESRVPVLDNEMLDVANTIPYQYKVRHFTPKHVFKKVAERHISRKIVYKKKVGFGVPVDEWLRDHSGLGRYL
DLLLDTARGINGVSRPRIEQLIAEHASRRYNHGDVLWPLVNYAIWREQFFK
>Mature_611_residues
MCGIVGKYYFNKNAGDSSDIDAMMQAIYHRGPDSSGKYINGRAALGFQRLSIIDTVSGHQPLYNETKTIVLLANGEIYNY
KEFVPFLESKGHVFSTRSDCEVIIHLYEEYGIEFISKLNGMFAFCLYDTRNDVMFIARDRVGIKPLYYHLNKEAIIFGSE
IKGILASGEVVTEEANDCLAEYLCFRYVANFRTFFSSVNALEPGTYIKISPNGCTFEKFWDLSKYVLNYAQDGDLVETVD
AALTASVQRQMMTDVPLGTQLSGGVDSSLVSKLAAGYSPGLKTFTVSFFESAYDESAYARLLADSAGLEYHQIRVDGKTF
ADTLPKVIWYHDEPLCHANSVHMYLLCTYARQFVTVLLTGEGADELFAGYPRYQICRFGDAYNKLNPSVASLVKSVLKGV
PARKIVKIVDNLGLGARELALWNSSFTTKEKVSWLLDSGDVTLDARSALVDSVWNDELSLFDNLLLYEQKSYLQPILMRQ
DKMSMAASVESRVPVLDNEMLDVANTIPYQYKVRHFTPKHVFKKVAERHISRKIVYKKKVGFGVPVDEWLRDHSGLGRYL
DLLLDTARGINGVSRPRIEQLIAEHASRRYNHGDVLWPLVNYAIWREQFFK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=406, Percent_Identity=30.7881773399015, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI168229252, Length=406, Percent_Identity=30.7881773399015, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI168229250, Length=406, Percent_Identity=30.7881773399015, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI296010848, Length=379, Percent_Identity=31.6622691292876, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI296010852, Length=308, Percent_Identity=30.8441558441558, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI296010850, Length=308, Percent_Identity=30.8441558441558, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786889, Length=393, Percent_Identity=31.8066157760814, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560178, Length=419, Percent_Identity=29.1169451073986, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71993933, Length=385, Percent_Identity=30.1298701298701, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=385, Percent_Identity=30.1298701298701, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=323, Percent_Identity=28.4829721362229, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=412, Percent_Identity=29.126213592233, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=413, Percent_Identity=28.0871670702179, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553209, Length=357, Percent_Identity=32.2128851540616, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69051; Mature: 69051

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGKYYFNKNAGDSSDIDAMMQAIYHRGPDSSGKYINGRAALGFQRLSIIDTVSGHQ
CCCCEEEHEECCCCCCCCCHHHHHHHHHHCCCCCCCCEECCHHHHCHHHHHHHHHCCCCC
PLYNETKTIVLLANGEIYNYKEFVPFLESKGHVFSTRSDCEVIIHLYEEYGIEFISKLNG
CCCCCCCEEEEEECCCEECHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
MFAFCLYDTRNDVMFIARDRVGIKPLYYHLNKEAIIFGSEIKGILASGEVVTEEANDCLA
EEEEEEEECCCCEEEEEECCCCCCCEEEEECCCEEEECCHHCHHHCCCCEEHHHHHHHHH
EYLCFRYVANFRTFFSSVNALEPGTYIKISPNGCTFEKFWDLSKYVLNYAQDGDLVETVD
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AALTASVQRQMMTDVPLGTQLSGGVDSSLVSKLAAGYSPGLKTFTVSFFESAYDESAYAR
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHH
LLADSAGLEYHQIRVDGKTFADTLPKVIWYHDEPLCHANSVHMYLLCTYARQFVTVLLTG
HHHHCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCCCCCEEHHHHHHHHHHHHEEEEEC
EGADELFAGYPRYQICRFGDAYNKLNPSVASLVKSVLKGVPARKIVKIVDNLGLGARELA
CCHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHE
LWNSSFTTKEKVSWLLDSGDVTLDARSALVDSVWNDELSLFDNLLLYEQKSYLQPILMRQ
EECCCCCCHHHHHHEECCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKMSMAASVESRVPVLDNEMLDVANTIPYQYKVRHFTPKHVFKKVAERHISRKIVYKKKV
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
GFGVPVDEWLRDHSGLGRYLDLLLDTARGINGVSRPRIEQLIAEHASRRYNHGDVLWPLV
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
NYAIWREQFFK
HHHHHHHHHCC
>Mature Secondary Structure
MCGIVGKYYFNKNAGDSSDIDAMMQAIYHRGPDSSGKYINGRAALGFQRLSIIDTVSGHQ
CCCCEEEHEECCCCCCCCCHHHHHHHHHHCCCCCCCCEECCHHHHCHHHHHHHHHCCCCC
PLYNETKTIVLLANGEIYNYKEFVPFLESKGHVFSTRSDCEVIIHLYEEYGIEFISKLNG
CCCCCCCEEEEEECCCEECHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
MFAFCLYDTRNDVMFIARDRVGIKPLYYHLNKEAIIFGSEIKGILASGEVVTEEANDCLA
EEEEEEEECCCCEEEEEECCCCCCCEEEEECCCEEEECCHHCHHHCCCCEEHHHHHHHHH
EYLCFRYVANFRTFFSSVNALEPGTYIKISPNGCTFEKFWDLSKYVLNYAQDGDLVETVD
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AALTASVQRQMMTDVPLGTQLSGGVDSSLVSKLAAGYSPGLKTFTVSFFESAYDESAYAR
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHH
LLADSAGLEYHQIRVDGKTFADTLPKVIWYHDEPLCHANSVHMYLLCTYARQFVTVLLTG
HHHHCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCCCCCEEHHHHHHHHHHHHEEEEEC
EGADELFAGYPRYQICRFGDAYNKLNPSVASLVKSVLKGVPARKIVKIVDNLGLGARELA
CCHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHE
LWNSSFTTKEKVSWLLDSGDVTLDARSALVDSVWNDELSLFDNLLLYEQKSYLQPILMRQ
EECCCCCCHHHHHHEECCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKMSMAASVESRVPVLDNEMLDVANTIPYQYKVRHFTPKHVFKKVAERHISRKIVYKKKV
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
GFGVPVDEWLRDHSGLGRYLDLLLDTARGINGVSRPRIEQLIAEHASRRYNHGDVLWPLV
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
NYAIWREQFFK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]