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The map label for this gene is paaK [H]

Identifier: 39996828

GI number: 39996828

Start: 1894220

End: 1895521

Strand: Reverse

Name: paaK [H]

Synonym: GSU1729

Alternate gene names: 39996828

Gene position: 1895521-1894220 (Counterclockwise)

Preceding gene: 39996829

Following gene: 308513306

Centisome position: 49.7

GC content: 58.29

Gene sequence:

>1302_bases
ATGCAGATCTGGGATCAACAGTACGAATGCATGCAGAGAGAGGAGCTGGAACAGCTCCAGCTTGAACGGCTCCAGGCAAC
ACTCAACAGGGCGTTCAAGAACGTTACCTGTTACCGCAACAAATTCACCGAACGAGGCATTGTTCCCGAAGACATCCAGT
CGCTGGACGACCTGAAGAAACTCCCGTTCACCACCAAGGAAGATCTGCGCCTGAACTACCCTTACGGCATGTTCGCGGTC
CCACTGCGGGAAGTGGTGCGGATTCATTCGTCATCCGGCACCACCGGCAAGCCGACTGTGGTCGGCTATACCAAGCACGA
CATCAAGGTCTGGTCCAACTTGGTGGCCCGCTTCATGACCGCTGCGGGTGTCACCCACGATGACGTGGTCCAGATCGCCT
TCGGCTACGGCTTGTTCACCGGCGCCTTCGGTCTTCACTACGGCGCGGAGGCCATTGGCGCATCGGTCATCCCCATGAGT
GCCGGCAACACCGAAAAGCAGATCATGATCATGCAGGACTACAAGACCACGGCCCTCGTCTGCACCCCCAGCTATGCCAT
CACCATTGCCGACCGGATGGAAAAGATGGGGGTCGATCCGAAAAGCCTTTCGCTGAAGGTCGGTCTCTTTGGGGCCGAGC
CCTGGTCTGAGGCAATGCGCAAGGAGATCGAGAACCGCCTCTTCGTCAGCGCCACCGACAACTATGGCCTGTCCGAAATC
ATCGGCCCGGGCGTGGCCGGCGACTGCGAGTACAAGTGCGGCATGCACATCTCAGAGGATGCTTTTATCGCGGAAATCAT
CGACCCTGAAACCGGGGAGGTGCTGCCGCCGGGAAGCGTCGGAGAGCTGGTGCTGACCACCCTCACCAAAGAAGCGTTTC
CCATGATCCGTTACCGGACCCGCGACATTACGAGTCTGGACTACTCGCCGTGCGCATGCGGCAGAACCAATGTGCGGATG
AAGAAGACCATGGGCCGCAGCGACGACATGCTTATCGTCAAGGGGGTTAATGTCTTCCCCTCCCAGATCGAAGAGATCCT
CTTCGCCATCGAGGGGTGCGAGCCCCATTACCAGATCGTTCTGGAGCGTCACGGAGCGCTGGATGTGCTCGAAGTGCACA
TCGAAGTGACCGAGAACATTTTCTTCGACGAGATGAAGAAGCAGAAGGCCTTCCTGGAGATGGTGGAAAAGCGGATAGAC
TCGGTCCTCGGCGTGGGGGCAACAGTGAAGCTCGTGGAGCCCAACAGCATTCCGCGCCACGAGGGCAAGGCCCAGCGGGT
CATAGACAACCGGAAAATCTGA

Upstream 100 bases:

>100_bases
GTGATACTTGAGGGAATAGCCTCTGAATTGCTTGAAAATAAAGAAGTTCAGCGCGCATACCTTGGTAAGGACAAAAAGGA
GATCTGGGAGAGATAACCGT

Downstream 100 bases:

>100_bases
CGGCGGTCACATGCCGATGCCCGCTATGGCGACGCCACAGTCGGGACAGGCTCCGTTTTCGATCCTGTTCCTTTCAATGA
GATACCCGTGGCGGCCGATC

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQIWDQQYECMQREELEQLQLERLQATLNRAFKNVTCYRNKFTERGIVPEDIQSLDDLKKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTHDDVVQIAFGYGLFTGAFGLHYGAEAIGASVIPMS
AGNTEKQIMIMQDYKTTALVCTPSYAITIADRMEKMGVDPKSLSLKVGLFGAEPWSEAMRKEIENRLFVSATDNYGLSEI
IGPGVAGDCEYKCGMHISEDAFIAEIIDPETGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITSLDYSPCACGRTNVRM
KKTMGRSDDMLIVKGVNVFPSQIEEILFAIEGCEPHYQIVLERHGALDVLEVHIEVTENIFFDEMKKQKAFLEMVEKRID
SVLGVGATVKLVEPNSIPRHEGKAQRVIDNRKI

Sequences:

>Translated_433_residues
MQIWDQQYECMQREELEQLQLERLQATLNRAFKNVTCYRNKFTERGIVPEDIQSLDDLKKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTHDDVVQIAFGYGLFTGAFGLHYGAEAIGASVIPMS
AGNTEKQIMIMQDYKTTALVCTPSYAITIADRMEKMGVDPKSLSLKVGLFGAEPWSEAMRKEIENRLFVSATDNYGLSEI
IGPGVAGDCEYKCGMHISEDAFIAEIIDPETGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITSLDYSPCACGRTNVRM
KKTMGRSDDMLIVKGVNVFPSQIEEILFAIEGCEPHYQIVLERHGALDVLEVHIEVTENIFFDEMKKQKAFLEMVEKRID
SVLGVGATVKLVEPNSIPRHEGKAQRVIDNRKI
>Mature_433_residues
MQIWDQQYECMQREELEQLQLERLQATLNRAFKNVTCYRNKFTERGIVPEDIQSLDDLKKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTHDDVVQIAFGYGLFTGAFGLHYGAEAIGASVIPMS
AGNTEKQIMIMQDYKTTALVCTPSYAITIADRMEKMGVDPKSLSLKVGLFGAEPWSEAMRKEIENRLFVSATDNYGLSEI
IGPGVAGDCEYKCGMHISEDAFIAEIIDPETGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITSLDYSPCACGRTNVRM
KKTMGRSDDMLIVKGVNVFPSQIEEILFAIEGCEPHYQIVLERHGALDVLEVHIEVTENIFFDEMKKQKAFLEMVEKRID
SVLGVGATVKLVEPNSIPRHEGKAQRVIDNRKI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=422, Percent_Identity=51.6587677725119, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48554; Mature: 48554

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIWDQQYECMQREELEQLQLERLQATLNRAFKNVTCYRNKFTERGIVPEDIQSLDDLKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMT
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVTHDDVVQIAFGYGLFTGAFGLHYGAEAIGASVIPMSAGNTEKQIMIMQDYKTTALV
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCEEEEEECCCCEEEE
CTPSYAITIADRMEKMGVDPKSLSLKVGLFGAEPWSEAMRKEIENRLFVSATDNYGLSEI
ECCCEEEEHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEECCCCCHHHH
IGPGVAGDCEYKCGMHISEDAFIAEIIDPETGEVLPPGSVGELVLTTLTKEAFPMIRYRT
CCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
RDITSLDYSPCACGRTNVRMKKTMGRSDDMLIVKGVNVFPSQIEEILFAIEGCEPHYQIV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHEEE
LERHGALDVLEVHIEVTENIFFDEMKKQKAFLEMVEKRIDSVLGVGATVKLVEPNSIPRH
EECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
EGKAQRVIDNRKI
CCHHHHHHCCCCC
>Mature Secondary Structure
MQIWDQQYECMQREELEQLQLERLQATLNRAFKNVTCYRNKFTERGIVPEDIQSLDDLKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMT
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVTHDDVVQIAFGYGLFTGAFGLHYGAEAIGASVIPMSAGNTEKQIMIMQDYKTTALV
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCEEEEEECCCCEEEE
CTPSYAITIADRMEKMGVDPKSLSLKVGLFGAEPWSEAMRKEIENRLFVSATDNYGLSEI
ECCCEEEEHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEECCCCCHHHH
IGPGVAGDCEYKCGMHISEDAFIAEIIDPETGEVLPPGSVGELVLTTLTKEAFPMIRYRT
CCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
RDITSLDYSPCACGRTNVRMKKTMGRSDDMLIVKGVNVFPSQIEEILFAIEGCEPHYQIV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHEEE
LERHGALDVLEVHIEVTENIFFDEMKKQKAFLEMVEKRIDSVLGVGATVKLVEPNSIPRH
EECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
EGKAQRVIDNRKI
CCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]