Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 39996771

GI number: 39996771

Start: 1831449

End: 1832417

Strand: Reverse

Name: pleD [H]

Synonym: GSU1671

Alternate gene names: 39996771

Gene position: 1832417-1831449 (Counterclockwise)

Preceding gene: 39996773

Following gene: 39996768

Centisome position: 48.04

GC content: 55.83

Gene sequence:

>969_bases
ATGACAGATGCCATTACGGATGAAACACGCGCGCCGCGCTTCCCTTTTCCCATTCTCATCGTTGACGACAACCGCCTGCT
GCGCACCATGCTGGAAACCAGCTTGAAATCTGCCGGTTACGATGTGGTAATGGCGGAAAACGGCAAAGAGGCCCTGGAAA
TCTTCAACAAGGGGTACTACCCTATCGTCATGACGGACTGGGTAATGCCGGAAATGGATGGGCTCGAACTCTGCCGGGCC
ATCCGCGCCGACTGTTCCGGCCGTTATACCTACATTATCCTCCTCACCTCCCAGGACTCGAAGAACGATGTCATCGCAGG
ACTTGAAGCGGGTGCAGACGAATATCTCGTCAAACCGGCCCACCAGGTTGAGCTGTTGACCCGTCTTAAAACCGCCAAGC
GAATCCTCGACCTGGAAAGCTCACTGAAAAAAAGCCTGGAAGAGATCGGCAACCTCTCCCGGATCGACACGTTGACCGGC
GTCTACAACCGCCGCTACCTGGATGAGCGCCTTTCCCCCGAAATCAAGCGAGCTTACCGCTATGAACGGGCACTGTCCCT
GATTCTTGTCGGGATTAACGACTTCAGCGGGATTACCTCTGCCCACGGACATTACGCGGGCGAGCTGGCTCTCAAGGGGT
GCGCCGACGTTCTCGCCGAATCGATCCGCAGGGACATCGACTGGCTGGCACGTTACGATGACGACAGATTCGTGGTTGTT
CTTCCTGAAACAGACGCCTCGGGAGCCATGATTCTCGCCAAGAGGCTCCGGATTCGGATTGCGTCGATGGCGCTGAAACT
GTACGACAAAGAGATCAAGGTCAATGCAGCCTTCGGGGTAGCCGGTTTTACCGCAACCCAGCAAAAAGAGGGAATGACGC
CCCACATTCTGCTCGACAAAGCGGACCGCTCATTGCGGGAAGCCTTGGAAGAAGGGGGTGAAATGATCAAGGGAGTCCAG
CTTCATTGA

Upstream 100 bases:

>100_bases
CAGGCAACGATGCTGTCAATGGTCTCAAAACCATCCGGAGTATCCTGATTGCACTAAAGAAATCACGCATTCTGCCGAAA
CGGTTGGAGAGGAGTCAACA

Downstream 100 bases:

>100_bases
TGGGCAAAAATATACCTATTCACCTGTAAAGAACCGCTTGATGCTCCTGCCCATTTCGCGGAAAGCCTCACCGGTTTTCC
TGCCGGTTTCCCGAAACCAC

Product: response regulator/GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTDAITDETRAPRFPFPILIVDDNRLLRTMLETSLKSAGYDVVMAENGKEALEIFNKGYYPIVMTDWVMPEMDGLELCRA
IRADCSGRYTYIILLTSQDSKNDVIAGLEAGADEYLVKPAHQVELLTRLKTAKRILDLESSLKKSLEEIGNLSRIDTLTG
VYNRRYLDERLSPEIKRAYRYERALSLILVGINDFSGITSAHGHYAGELALKGCADVLAESIRRDIDWLARYDDDRFVVV
LPETDASGAMILAKRLRIRIASMALKLYDKEIKVNAAFGVAGFTATQQKEGMTPHILLDKADRSLREALEEGGEMIKGVQ
LH

Sequences:

>Translated_322_residues
MTDAITDETRAPRFPFPILIVDDNRLLRTMLETSLKSAGYDVVMAENGKEALEIFNKGYYPIVMTDWVMPEMDGLELCRA
IRADCSGRYTYIILLTSQDSKNDVIAGLEAGADEYLVKPAHQVELLTRLKTAKRILDLESSLKKSLEEIGNLSRIDTLTG
VYNRRYLDERLSPEIKRAYRYERALSLILVGINDFSGITSAHGHYAGELALKGCADVLAESIRRDIDWLARYDDDRFVVV
LPETDASGAMILAKRLRIRIASMALKLYDKEIKVNAAFGVAGFTATQQKEGMTPHILLDKADRSLREALEEGGEMIKGVQ
LH
>Mature_321_residues
TDAITDETRAPRFPFPILIVDDNRLLRTMLETSLKSAGYDVVMAENGKEALEIFNKGYYPIVMTDWVMPEMDGLELCRAI
RADCSGRYTYIILLTSQDSKNDVIAGLEAGADEYLVKPAHQVELLTRLKTAKRILDLESSLKKSLEEIGNLSRIDTLTGV
YNRRYLDERLSPEIKRAYRYERALSLILVGINDFSGITSAHGHYAGELALKGCADVLAESIRRDIDWLARYDDDRFVVVL
PETDASGAMILAKRLRIRIASMALKLYDKEIKVNAAFGVAGFTATQQKEGMTPHILLDKADRSLREALEEGGEMIKGVQL
H

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788191, Length=113, Percent_Identity=38.9380530973451, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=33.6134453781513, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082012, Length=147, Percent_Identity=31.2925170068027, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=29.9145299145299, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=38.135593220339, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=161, Percent_Identity=29.8136645962733, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=35.042735042735, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=35.0877192982456, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790552, Length=118, Percent_Identity=36.4406779661017, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788394, Length=147, Percent_Identity=31.9727891156463, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787229, Length=125, Percent_Identity=32.8, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=35.2941176470588, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693134, Length=142, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367329, Length=117, Percent_Identity=35.042735042735, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789402, Length=145, Percent_Identity=29.6551724137931, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788905, Length=105, Percent_Identity=37.1428571428571, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788521, Length=122, Percent_Identity=34.4262295081967, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=102, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787487, Length=114, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=34.1880341880342, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36122; Mature: 35991

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAITDETRAPRFPFPILIVDDNRLLRTMLETSLKSAGYDVVMAENGKEALEIFNKGYY
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCC
PIVMTDWVMPEMDGLELCRAIRADCSGRYTYIILLTSQDSKNDVIAGLEAGADEYLVKPA
EEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHCCCH
HQVELLTRLKTAKRILDLESSLKKSLEEIGNLSRIDTLTGVYNRRYLDERLSPEIKRAYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YERALSLILVGINDFSGITSAHGHYAGELALKGCADVLAESIRRDIDWLARYDDDRFVVV
HHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LPETDASGAMILAKRLRIRIASMALKLYDKEIKVNAAFGVAGFTATQQKEGMTPHILLDK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCEEEEEH
ADRSLREALEEGGEMIKGVQLH
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDAITDETRAPRFPFPILIVDDNRLLRTMLETSLKSAGYDVVMAENGKEALEIFNKGYY
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCC
PIVMTDWVMPEMDGLELCRAIRADCSGRYTYIILLTSQDSKNDVIAGLEAGADEYLVKPA
EEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHCCCH
HQVELLTRLKTAKRILDLESSLKKSLEEIGNLSRIDTLTGVYNRRYLDERLSPEIKRAYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YERALSLILVGINDFSGITSAHGHYAGELALKGCADVLAESIRRDIDWLARYDDDRFVVV
HHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LPETDASGAMILAKRLRIRIASMALKLYDKEIKVNAAFGVAGFTATQQKEGMTPHILLDK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCEEEEEH
ADRSLREALEEGGEMIKGVQLH
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA