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The map label for this gene is kefA [C]

Identifier: 39996733

GI number: 39996733

Start: 1790185

End: 1791279

Strand: Direct

Name: kefA [C]

Synonym: GSU1633

Alternate gene names: 39996733

Gene position: 1790185-1791279 (Clockwise)

Preceding gene: 39996732

Following gene: 39996734

Centisome position: 46.94

GC content: 55.98

Gene sequence:

>1095_bases
GTGGCTGACATCATAGCGTTTCTCAGCAACTTCTACCGGTTGGAGGCAGGAGATCATCTGCTGCTCTTCTGGCTGAAAGA
AGCCCTCATCGCCCTGATCATCTTCAGCATTTTCTGGGGGCTGTCTCTGTTGCTCCGGACCCTGCTCACCAGATGGGTTC
CCCGGTTCACCGCGTTTACGAGCACCGACCTGGATGACCGGATTCTGGCTCGCGTGACGCCGCCGGCATGCCTGCTGGTG
GTCCTGGCGGGTCTCTACTATGCGGTCAAGTCGTTGCCGTTTCCTGAGAAGGCCCATGTGCTCAGTGCCGGGGCACTATT
CATCGTTAACGTCATTGTTGTTACCAATATTGCCTGGCGAATCACAAACGAGCTATTAGGGTGGTACGGGAGCCGTCTTG
CTGAGCGCCACGGCGGAGGAGTCGATCGGCAGATCATACCCCCGCTGGAAAAGCTCATCACCATCTTCCTGGCAGGTATC
GCCCTGATGGTGGTGCTCAGACATTTCAATTACGACATCCTCTCGGTGGTGACGGCACTGGGAATCGGCTCCCTTGCCAT
CGGTATGGCGGCAAAGGAAACCCTGGCCAACATGATCTCAGGTTTTACCCTGATGGTGGACCGCCCTTTCCGTATTGGCG
ACCGGATTCAGCTTGCCTCCGGCCAATGGGGCGATGTAGCCGATATCGGGCTGAGGACCACCAAGATCAAAACTGTGGAC
AATACGCTCCTGATCATTCCCAATTCGGAGCTCTGTAACACAACCATCATCAACCTTGCGTTTCCCGACGTCCGGGCCAA
GGGACGGATCAACGTCGGTATTTCCTACGACAATGATGTCGAGCGGGCCAAGTCGCTGCTGGTCGAAACTGCCCTTTCGG
TTCCGGAGGTGCTGCGGGAGCCGGCGCCGGAGGCGTTTTTCGTGTCGTTCGGCGACAGCGCTCTGAATGTGTCGCTCTTC
TTCTGGGTAAGTGAATACGGCTCTGTCTTTGCTACCACCGATAAAATCAATGAACAGATCATCGCACGGTTCCGCGAGGA
AGGGATCACCATTCCCTATCCGATACGGACGGTCATCATGGAAAAGGAACAGTAA

Upstream 100 bases:

>100_bases
GGTGAAGTGCGCGGTTTCCTCCCGGAGGATGAAATCCGTGAGGCTTTTGACCTCAACTACCACCTGAAGCATGTGAACAC
CATCTTCACGAGGGTCTTCG

Downstream 100 bases:

>100_bases
ATGGCGCAACGAATCGAAGTCGCCCTGAAAGAGGGAGTTCGCGATGCCCGCGGCGAGCGGGTTCGACGGGAGATTGAGCA
TTTTCTCCACCTGCCGGTGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MADIIAFLSNFYRLEAGDHLLLFWLKEALIALIIFSIFWGLSLLLRTLLTRWVPRFTAFTSTDLDDRILARVTPPACLLV
VLAGLYYAVKSLPFPEKAHVLSAGALFIVNVIVVTNIAWRITNELLGWYGSRLAERHGGGVDRQIIPPLEKLITIFLAGI
ALMVVLRHFNYDILSVVTALGIGSLAIGMAAKETLANMISGFTLMVDRPFRIGDRIQLASGQWGDVADIGLRTTKIKTVD
NTLLIIPNSELCNTTIINLAFPDVRAKGRINVGISYDNDVERAKSLLVETALSVPEVLREPAPEAFFVSFGDSALNVSLF
FWVSEYGSVFATTDKINEQIIARFREEGITIPYPIRTVIMEKEQ

Sequences:

>Translated_364_residues
MADIIAFLSNFYRLEAGDHLLLFWLKEALIALIIFSIFWGLSLLLRTLLTRWVPRFTAFTSTDLDDRILARVTPPACLLV
VLAGLYYAVKSLPFPEKAHVLSAGALFIVNVIVVTNIAWRITNELLGWYGSRLAERHGGGVDRQIIPPLEKLITIFLAGI
ALMVVLRHFNYDILSVVTALGIGSLAIGMAAKETLANMISGFTLMVDRPFRIGDRIQLASGQWGDVADIGLRTTKIKTVD
NTLLIIPNSELCNTTIINLAFPDVRAKGRINVGISYDNDVERAKSLLVETALSVPEVLREPAPEAFFVSFGDSALNVSLF
FWVSEYGSVFATTDKINEQIIARFREEGITIPYPIRTVIMEKEQ
>Mature_363_residues
ADIIAFLSNFYRLEAGDHLLLFWLKEALIALIIFSIFWGLSLLLRTLLTRWVPRFTAFTSTDLDDRILARVTPPACLLVV
LAGLYYAVKSLPFPEKAHVLSAGALFIVNVIVVTNIAWRITNELLGWYGSRLAERHGGGVDRQIIPPLEKLITIFLAGIA
LMVVLRHFNYDILSVVTALGIGSLAIGMAAKETLANMISGFTLMVDRPFRIGDRIQLASGQWGDVADIGLRTTKIKTVDN
TLLIIPNSELCNTTIINLAFPDVRAKGRINVGISYDNDVERAKSLLVETALSVPEVLREPAPEAFFVSFGDSALNVSLFF
WVSEYGSVFATTDKINEQIIARFREEGITIPYPIRTVIMEKEQ

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=278, Percent_Identity=28.7769784172662, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789291, Length=267, Percent_Identity=26.9662921348315, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787591, Length=226, Percent_Identity=30.5309734513274, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI2367355, Length=179, Percent_Identity=29.0502793296089, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 40429; Mature: 40298

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIIAFLSNFYRLEAGDHLLLFWLKEALIALIIFSIFWGLSLLLRTLLTRWVPRFTAFT
CHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
STDLDDRILARVTPPACLLVVLAGLYYAVKSLPFPEKAHVLSAGALFIVNVIVVTNIAWR
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
ITNELLGWYGSRLAERHGGGVDRQIIPPLEKLITIFLAGIALMVVLRHFNYDILSVVTAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GIGSLAIGMAAKETLANMISGFTLMVDRPFRIGDRIQLASGQWGDVADIGLRTTKIKTVD
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCCHHHCCCEEEEEEEEC
NTLLIIPNSELCNTTIINLAFPDVRAKGRINVGISYDNDVERAKSLLVETALSVPEVLRE
CEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
PAPEAFFVSFGDSALNVSLFFWVSEYGSVFATTDKINEQIIARFREEGITIPYPIRTVIM
CCCCEEEEEECCCCEEEEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHEEEE
EKEQ
CCCC
>Mature Secondary Structure 
ADIIAFLSNFYRLEAGDHLLLFWLKEALIALIIFSIFWGLSLLLRTLLTRWVPRFTAFT
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
STDLDDRILARVTPPACLLVVLAGLYYAVKSLPFPEKAHVLSAGALFIVNVIVVTNIAWR
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
ITNELLGWYGSRLAERHGGGVDRQIIPPLEKLITIFLAGIALMVVLRHFNYDILSVVTAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GIGSLAIGMAAKETLANMISGFTLMVDRPFRIGDRIQLASGQWGDVADIGLRTTKIKTVD
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCCHHHCCCEEEEEEEEC
NTLLIIPNSELCNTTIINLAFPDVRAKGRINVGISYDNDVERAKSLLVETALSVPEVLRE
CEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
PAPEAFFVSFGDSALNVSLFFWVSEYGSVFATTDKINEQIIARFREEGITIPYPIRTVIM
CCCCEEEEEECCCCEEEEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHEEEE
EKEQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]