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The map label for this gene is purB [H]

Identifier: 39996732

GI number: 39996732

Start: 1788897

End: 1790192

Strand: Direct

Name: purB [H]

Synonym: GSU1632

Alternate gene names: 39996732

Gene position: 1788897-1790192 (Clockwise)

Preceding gene: 39996731

Following gene: 39996733

Centisome position: 46.9

GC content: 58.87

Gene sequence:

>1296_bases
GTGATCGAACGTTACAGCCGTCCCGAAATGGCCCGTATCTGGGAACCGCGCAATCGCTACCAGAAGTGGCTCGACATCGA
GATTTACGCCTGCGAGGCCCACGCTGACCTGGGCAACGTGCCCCGCGACGCGGTAGAGCGCATCAAGGCAAAGGCTGACT
TCGACGTAGACCGCATTGATGAGATCGAGCGGACGGTGAAGCACGACGTCATCGCCTTTCTCACCTCCGTTGCCGACTAC
ATCGGAGATGATTCACGCTTTGTCCACTTGGGACTCACCTCGTCGGACGTGCTCGATACCTCGTTCGCCATGCTGCTGGC
CGAGGCCTCCGATCTCATCATCGATGACATCAAGCGGCTCATGGAGGTCATTAAGCGCCGCGCCTACGAACACAAGGATA
CGCCGATGATGGGGCGTTCCCACGGCATCCATGCCGAACCGGTCACCTTCGGCATCAAGATGGCTCTCTGGTACGACGAG
ATGAAGCGCAATCTCCGCCGGATGGAGGCTGCCCGGGAAACCATTGCCTACGGCAAGATATCCGGGGCGGTCGGCACCTT
TGCCAACATTGATCCGATGGTAGAGGAGGCTGTTTGCGCCAAGGCAGGGCTCAAGCCGGCTCCCTGTTCGACCCAGGTGA
TCCAGCGGGACCGCCACGCGGAGTTCTTCTCCACCCTGGCGATCATCGCCACCTCCATCGAGAAGTTTGCCGTGGAGATT
CGTCATCTCCAGCGGACCGAAGTGCTCGAGGCCGAAGAGTTTTTCAGCAAGGGGCAAAAGGGGTCGTCAGCCATGCCCCA
CAAACGCAATCCCGTCCTCTCCGAGAACCTGACCGGTCTTGCACGGCTCATGCGGGGCTATTCGGTATCCGCCCTCGAAA
ACGTACCCCTCTGGCACGAGCGGGACATCTCTCACTCGTCCGTCGAGCGAGTCATCGGCCCGGATGCTACCATCTGTCTC
GATTTCATGCTCAACCGGACCATCGGCCTCATCGACAATCTCGTGGTTTACCCCGAGAACATGATGCGGAACCTGAATCT
CATGCGCGGACTCATTTTCTCTCAGCGGGTGCTCCTTAAGTTGGCCACTGCCGGTGCGTCCCGCGAGAAGGCCTATGAAC
TGGTCCAGCGCAATGCTATGAAGGTGTGGGAGCAGGGCAAGGATTTCCAGGAAGAACTTCTGGCCGACGGTGAAGTGCGC
GGTTTCCTCCCGGAGGATGAAATCCGTGAGGCTTTTGACCTCAACTACCACCTGAAGCATGTGAACACCATCTTCACGAG
GGTCTTCGGTGGCTGA

Upstream 100 bases:

>100_bases
AACAGTCTGCTGACCGACCCGCGCAGGATGGACGCTTCGTGTATTCCGACGCCTGTCAGGGACTGCACATACTTTACTGA
ACGAAAGGAAGGACCCGCCC

Downstream 100 bases:

>100_bases
CATCATAGCGTTTCTCAGCAACTTCTACCGGTTGGAGGCAGGAGATCATCTGCTGCTCTTCTGGCTGAAAGAAGCCCTCA
TCGCCCTGATCATCTTCAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYSRPEMARIWEPRNRYQKWLDIEIYACEAHADLGNVPRDAVERIKAKADFDVDRIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAYEHKDTPMMGRSHGIHAEPVTFGIKMALWYDE
MKRNLRRMEAARETIAYGKISGAVGTFANIDPMVEEAVCAKAGLKPAPCSTQVIQRDRHAEFFSTLAIIATSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLMRGYSVSALENVPLWHERDISHSSVERVIGPDATICL
DFMLNRTIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLATAGASREKAYELVQRNAMKVWEQGKDFQEELLADGEVR
GFLPEDEIREAFDLNYHLKHVNTIFTRVFGG

Sequences:

>Translated_431_residues
MIERYSRPEMARIWEPRNRYQKWLDIEIYACEAHADLGNVPRDAVERIKAKADFDVDRIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAYEHKDTPMMGRSHGIHAEPVTFGIKMALWYDE
MKRNLRRMEAARETIAYGKISGAVGTFANIDPMVEEAVCAKAGLKPAPCSTQVIQRDRHAEFFSTLAIIATSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLMRGYSVSALENVPLWHERDISHSSVERVIGPDATICL
DFMLNRTIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLATAGASREKAYELVQRNAMKVWEQGKDFQEELLADGEVR
GFLPEDEIREAFDLNYHLKHVNTIFTRVFGG
>Mature_431_residues
MIERYSRPEMARIWEPRNRYQKWLDIEIYACEAHADLGNVPRDAVERIKAKADFDVDRIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAYEHKDTPMMGRSHGIHAEPVTFGIKMALWYDE
MKRNLRRMEAARETIAYGKISGAVGTFANIDPMVEEAVCAKAGLKPAPCSTQVIQRDRHAEFFSTLAIIATSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLMRGYSVSALENVPLWHERDISHSSVERVIGPDATICL
DFMLNRTIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLATAGASREKAYELVQRNAMKVWEQGKDFQEELLADGEVR
GFLPEDEIREAFDLNYHLKHVNTIFTRVFGG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=27.9373368146214, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1787376, Length=311, Percent_Identity=30.8681672025724, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17508577, Length=398, Percent_Identity=27.6381909547739, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=342, Percent_Identity=29.8245614035088, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=404, Percent_Identity=28.2178217821782, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24647570, Length=405, Percent_Identity=27.4074074074074, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49148; Mature: 49148

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPEMARIWEPRNRYQKWLDIEIYACEAHADLGNVPRDAVERIKAKADFDVDRID
CCCCCCCCCHHHHCCHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHH
EIERTVKHDVIAFLTSVADYIGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MEVIKRRAYEHKDTPMMGRSHGIHAEPVTFGIKMALWYDEMKRNLRRMEAARETIAYGKI
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPMVEEAVCAKAGLKPAPCSTQVIQRDRHAEFFSTLAIIATSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLMRGYSVSALENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCC
RDISHSSVERVIGPDATICLDFMLNRTIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LATAGASREKAYELVQRNAMKVWEQGKDFQEELLADGEVRGFLPEDEIREAFDLNYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHH
VNTIFTRVFGG
HHHHHHHHHCC
>Mature Secondary Structure
MIERYSRPEMARIWEPRNRYQKWLDIEIYACEAHADLGNVPRDAVERIKAKADFDVDRID
CCCCCCCCCHHHHCCHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHH
EIERTVKHDVIAFLTSVADYIGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MEVIKRRAYEHKDTPMMGRSHGIHAEPVTFGIKMALWYDEMKRNLRRMEAARETIAYGKI
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPMVEEAVCAKAGLKPAPCSTQVIQRDRHAEFFSTLAIIATSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLMRGYSVSALENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCC
RDISHSSVERVIGPDATICLDFMLNRTIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LATAGASREKAYELVQRNAMKVWEQGKDFQEELLADGEVRGFLPEDEIREAFDLNYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHH
VNTIFTRVFGG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]