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The map label for this gene is nth [H]

Identifier: 39996550

GI number: 39996550

Start: 1587992

End: 1588621

Strand: Direct

Name: nth [H]

Synonym: GSU1450

Alternate gene names: 39996550

Gene position: 1587992-1588621 (Clockwise)

Preceding gene: 39996549

Following gene: 39996551

Centisome position: 41.63

GC content: 57.46

Gene sequence:

>630_bases
ATGGCCGGCTTGGCTGAGGCCGTGAAACAGTGGCCGTCCCCCGCCGTTACCATCGTTTCGCAGCGGGAGGGCAATCCCTT
CAAGGTCCTCGTCTCCTGCATCCTGTCGCTGCGCACCCAGGACCGGACTACCGGACCGGCATCGGAGCGTCTCTTCGCTC
TGGCGGATACACCCGCCGCAATGGTGCGTTTGTCAAAGGACGACATCGAGAAGGCTATCTATCCTGTGGGTTTTTACCAC
ACCAAGGCTGAACAGATCCTCGAAATCTGCCGTGTTCTTCTGGAACGGTACGACGGCGGAGTTCCCGATGAACTCGATGA
GCTTCTCGCATTCAAGGGGGTCGGGCGCAAAACGGCAAACTTGGTGATCACTCTCGGTTTCGGGAAGCCGGGAATCTGCG
TCGACACCCACGTCCACCGAATCTGCAACCGCTGGGGATACATCAGGACCAAGACTCCTGAGCAAACGGAGTTTTCTCTG
CGTCGTATCCTTCCGCATCGCTATTGGCTGGTCATTAACGACTACCTTGTTACCTTTGGCCAGAATCACTGCACTCCCGT
TTCCCCCCGCTGTTCAACTTGTGTCCTTGCCCAATGGTGCGACTGCGTAGGGGTTGCGAAGCATCGATAG

Upstream 100 bases:

>100_bases
TTTTCTGCGTGGGAATATCGCTGTCGATCCTGGGTGTACTTCTGATGCGCATCTTCAGGAACCGGGGATTTTCATGAAAG
AAAGCGATATCCACGTCGTC

Downstream 100 bases:

>100_bases
TGCCGCGGAGCCGGTAATGTACGAAAATCATTTGCCGATGGAAACATTTAACGGTATCTTTTTTTCAGTTTGATGTGCCG
TATTTTTTAAACTGGACTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAGLAEAVKQWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFALADTPAAMVRLSKDDIEKAIYPVGFYH
TKAEQILEICRVLLERYDGGVPDELDELLAFKGVGRKTANLVITLGFGKPGICVDTHVHRICNRWGYIRTKTPEQTEFSL
RRILPHRYWLVINDYLVTFGQNHCTPVSPRCSTCVLAQWCDCVGVAKHR

Sequences:

>Translated_209_residues
MAGLAEAVKQWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFALADTPAAMVRLSKDDIEKAIYPVGFYH
TKAEQILEICRVLLERYDGGVPDELDELLAFKGVGRKTANLVITLGFGKPGICVDTHVHRICNRWGYIRTKTPEQTEFSL
RRILPHRYWLVINDYLVTFGQNHCTPVSPRCSTCVLAQWCDCVGVAKHR
>Mature_208_residues
AGLAEAVKQWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFALADTPAAMVRLSKDDIEKAIYPVGFYHT
KAEQILEICRVLLERYDGGVPDELDELLAFKGVGRKTANLVITLGFGKPGICVDTHVHRICNRWGYIRTKTPEQTEFSLR
RILPHRYWLVINDYLVTFGQNHCTPVSPRCSTCVLAQWCDCVGVAKHR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=41.2429378531073, Blast_Score=137, Evalue=8e-33,
Organism=Escherichia coli, GI1787920, Length=178, Percent_Identity=37.6404494382022, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1789331, Length=189, Percent_Identity=26.984126984127, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=170, Percent_Identity=43.5294117647059, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319304, Length=168, Percent_Identity=34.5238095238095, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=31.3829787234043, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=37.8531073446328, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23440; Mature: 23309

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLAEAVKQWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFALADTPAA
CCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHCEEEEECCCHH
MVRLSKDDIEKAIYPVGFYHTKAEQILEICRVLLERYDGGVPDELDELLAFKGVGRKTAN
HEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCE
LVITLGFGKPGICVDTHVHRICNRWGYIRTKTPEQTEFSLRRILPHRYWLVINDYLVTFG
EEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHCC
QNHCTPVSPRCSTCVLAQWCDCVGVAKHR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGLAEAVKQWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFALADTPAA
CCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHCEEEEECCCHH
MVRLSKDDIEKAIYPVGFYHTKAEQILEICRVLLERYDGGVPDELDELLAFKGVGRKTAN
HEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCE
LVITLGFGKPGICVDTHVHRICNRWGYIRTKTPEQTEFSLRRILPHRYWLVINDYLVTFG
EEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHCC
QNHCTPVSPRCSTCVLAQWCDCVGVAKHR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]