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The map label for this gene is ispB [H]

Identifier: 39996419

GI number: 39996419

Start: 1443412

End: 1444380

Strand: Direct

Name: ispB [H]

Synonym: GSU1317

Alternate gene names: 39996419

Gene position: 1443412-1444380 (Clockwise)

Preceding gene: 39996411

Following gene: 39996420

Centisome position: 37.84

GC content: 58.31

Gene sequence:

>969_bases
ATGCACGCTGCTCTTTCCCTGGTCGGGGATGACCTGAAAAACGTCGAATTACAGTTCAGAAAGGACCTGGAATCAGATGT
CTATCTGATCCGCAAGGTGGGAGAGTATGTCCTTGCCAGCGGCGGCAAGAGAATTCGTCCCATGCTGTTGCTCCTTTCGG
CTAAGCTGTGCGGCTACCAGGGCGATCGCCACGTTCCCCTCGCCAGCGTCATCGAATTCATCCACACCGCAACCCTGCTC
CACGACGACGTGGTCGACAACGCCACGTTGCGCCGCGGCAACGCCTCCGCCAACGAGGTCTGGGGCAATGAGGCCTCGGT
ACTGGTGGGCGACTTTCTCTTTTCCAAGTCCTTTTCTCTCATGGTAGAGGCCGGGGATCTGCGGATTCTCAAGGTCATAT
CCGGTGCCACGACGATCATCGCCGAGGGAGAGGTCCTGCAGCTCCTCTGCACCAGTGATCTGGAGATGACCCAGGAGCGC
TACATCGAGGTGGTCAAGAGCAAGACCGCCGTGCTCCTTTCAGCCGCCTGCGAGGCCGGTGCCATCCTGGGCACGTCGTC
GGCCGAGCAGCAGGAGGCACTGCGCGACTTCGGCATGGACCTGGGCATAGCCTTCCAGCTCATGGACGACACCCTCGACT
ACACGGCCAGCGAAGAGCAATTCGGCAAGGAAATCGGCCACGATCTCGAGGAGGGGAAAATCACGCTCCCTCTTATCCAT
ACCCTCATGAAGTGCTCCGACGAAGAGCGCGAAACCATCGCCGCCGTAGTGGAAAAGGAAATCCTGGAGCCGGGTGACTT
CGAATCGGTGTTCACCCTCGTCCACAAGTATGGCGGCATCGAGTACACCGTCTCCGTTGCCAACGAGTATATCGCCCGCT
GCAAGGCCCACCTTGGCGCATTTGTTGATTCACAGGAAAAAGCCGCACTGATTGAACTTGCCGATTATGTGGTGAATCGC
AAGCGCTAA

Upstream 100 bases:

>100_bases
TCGACTGGTTTACGCTATCCGCAAGAAGAACGGAGAGAAATGCTTTGACAAAAAAGGCGTAATTACCTAGTATTGGCGAA
AATTTACCCGAGGTTGCCTG

Downstream 100 bases:

>100_bases
ATAACCGTACACTTTGGTCAAATAACGCATCATTCCGATTTGCCGTTGTTTTTTGCCAGTCATTTCCGTTGCTCACGGAT
TGGCCCGCATATTGCTCACT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MHAALSLVGDDLKNVELQFRKDLESDVYLIRKVGEYVLASGGKRIRPMLLLLSAKLCGYQGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVEAGDLRILKVISGATTIIAEGEVLQLLCTSDLEMTQER
YIEVVKSKTAVLLSAACEAGAILGTSSAEQQEALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLMKCSDEERETIAAVVEKEILEPGDFESVFTLVHKYGGIEYTVSVANEYIARCKAHLGAFVDSQEKAALIELADYVVNR
KR

Sequences:

>Translated_322_residues
MHAALSLVGDDLKNVELQFRKDLESDVYLIRKVGEYVLASGGKRIRPMLLLLSAKLCGYQGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVEAGDLRILKVISGATTIIAEGEVLQLLCTSDLEMTQER
YIEVVKSKTAVLLSAACEAGAILGTSSAEQQEALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLMKCSDEERETIAAVVEKEILEPGDFESVFTLVHKYGGIEYTVSVANEYIARCKAHLGAFVDSQEKAALIELADYVVNR
KR
>Mature_322_residues
MHAALSLVGDDLKNVELQFRKDLESDVYLIRKVGEYVLASGGKRIRPMLLLLSAKLCGYQGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVEAGDLRILKVISGATTIIAEGEVLQLLCTSDLEMTQER
YIEVVKSKTAVLLSAACEAGAILGTSSAEQQEALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLMKCSDEERETIAAVVEKEILEPGDFESVFTLVHKYGGIEYTVSVANEYIARCKAHLGAFVDSQEKAALIELADYVVNR
KR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=319, Percent_Identity=30.7210031347962, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4758430, Length=279, Percent_Identity=22.9390681003584, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83700220, Length=279, Percent_Identity=22.9390681003584, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=45, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1786623, Length=226, Percent_Identity=28.7610619469027, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17505681, Length=321, Percent_Identity=29.9065420560748, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=29.9003322259136, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=222, Percent_Identity=25.2252252252252, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=306, Percent_Identity=32.3529411764706, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=278, Percent_Identity=21.9424460431655, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24660002, Length=278, Percent_Identity=21.9424460431655, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35377; Mature: 35377

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAALSLVGDDLKNVELQFRKDLESDVYLIRKVGEYVLASGGKRIRPMLLLLSAKLCGYQ
CCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDRHVPLASVIEFIHTATLLHDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
MVEAGDLRILKVISGATTIIAEGEVLQLLCTSDLEMTQERYIEVVKSKTAVLLSAACEAG
EEECCCEEEEEECCCCEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AILGTSSAEQQEALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
CEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCEEHHHHH
TLMKCSDEERETIAAVVEKEILEPGDFESVFTLVHKYGGIEYTVSVANEYIARCKAHLGA
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
FVDSQEKAALIELADYVVNRKR
HCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHAALSLVGDDLKNVELQFRKDLESDVYLIRKVGEYVLASGGKRIRPMLLLLSAKLCGYQ
CCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDRHVPLASVIEFIHTATLLHDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
MVEAGDLRILKVISGATTIIAEGEVLQLLCTSDLEMTQERYIEVVKSKTAVLLSAACEAG
EEECCCEEEEEECCCCEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AILGTSSAEQQEALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
CEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCEEHHHHH
TLMKCSDEERETIAAVVEKEILEPGDFESVFTLVHKYGGIEYTVSVANEYIARCKAHLGA
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
FVDSQEKAALIELADYVVNRKR
HCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]