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The map label for this gene is gltX

Identifier: 39996321

GI number: 39996321

Start: 1322000

End: 1323400

Strand: Direct

Name: gltX

Synonym: GSU1219

Alternate gene names: 39996321

Gene position: 1322000-1323400 (Clockwise)

Preceding gene: 39996320

Following gene: 39996324

Centisome position: 34.66

GC content: 61.74

Gene sequence:

>1401_bases
ATGTCCGACGTACGTTTGCGCTTCGCGCCGAGCCCCACGGGCTATCTCCACGTGGGGGGAGCGCGTACCGCACTTTTCAA
TTGGCTGCTGGCCCGCAAGCAGGGCGGTACATTCATCCTGCGCATCGAGGATACCGATGTTGCCCGTTCCACCCAGGAAT
CGGTTGATGCCATCCTCCAGGGGATGGAATGGCTCGGCCTCGACTGGGATGAGGGGCCTTTCTATCAATCCGAGCGCTTT
CCCGTCTACCGGGAGTTCGTGGAACGGCTCCTGGCCGAGGGAAAGGCGTACCGCTGCTACTGCACGCCCGAGGAGTTGGA
GGCGCGGCGCGAACAGGCCATGAAGGAGGGGCGCAAGCCCAAGTATGACGGTACCTGCCGCGATCTTGTCACTCAGGACG
CCGACAGGCCCTTTGTGGTCCGCTTCAGGGCCCCCCACGAAGGGGTGACAGCCTTCGACGATCTGATCAAAGGCCGGATC
GCCTTTAACAACGAGGAGCTGGACGACCTGATCATACAGCGCACCGACGGAACCCCCACCTATAACTTCGTGGTGGTTAT
CGACGATGCAACCATGGGGATCACCACCGTCATCCGCGGTGACGATCATATCAACAACACTCCCCGCCAGATTCTGCTCT
ACGAGGCCCTGGGCTACCCGGTGCCCCGTTTTGCCCACGTGCCGATGATCCTCGGCGCCGACAAGACCCGGCTTTCCAAA
CGGCATGGCGCCACGAGCGTCATGGCTTACCGCGACATGGGGTTTCTGCCCGAAGCGATGGTCAATTACCTGGTGCGGCT
CGGCTGGAGCCACGGGGACGAAGAGATATTCAGCCGGCAGGACCTTGTCGAGAAGTTCACGATTGAAGCGGTGGGCAAGT
CTGCAGGGGTGTTCAATCCGGACAAGCTCCTCTGGCTCAACCATCACTACATCAAAGAGAGCTCGCCCGAGCGACTCGCG
GAGCTTCTGGTGCCGTTTCTCCGGGAGCGGGGTGTCGACCCCTCGACCGGACCGAGCCTCCCTCAAGTGGTCAAGACTCT
TCAGGAGCGGTCGCGGACTCTCGTTGAGATGGCCGACGGTGCCCTGTTCTACTATCGGAGCGAAATCAGCTATGACGAGG
AGGCAGCCGCCAAGCATCTGGTGCCGGGGACGCTTCCTCTGCTTGCCTCGCTCGCGGAGAAACTCGACGCGTGTGCCGAT
TTCAGCCATTCGGGGCTCGAGGCCGCATTCAAGGAGTTCATTGCGGAAAAGGGGATCAAATTCGGGCAACTGGGACCGGC
CGTGCGCGTTTCTCTGTGCGGCGGCACCGCTTCACCCGGTATCTACGAGGTTGTAGAGGCTCTCGGCAAGGACGAGACGC
TGCGGCGGCTGCGCCGGGCCGTGGCATATCTGGAGCAGTAG

Upstream 100 bases:

>100_bases
ACCGTTGATCTGGCCGCCTGGGCCGACGAGGAGTATCGCATCGCCGGTCCCGAAGCCCCTTCCGACAACGACGAAAACGA
CCGAGAGTAAGGAGCACTTC

Downstream 100 bases:

>100_bases
ACCGAACCATTTCGCGCACTGCAAAAGGGGCGGCACGATCGGATTCGTGCCGCCCCTTTTTTGTCGAAATGTTCTGCTGC
CCCGGCGACCCGCCGGTGCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDVRLRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVARSTQESVDAILQGMEWLGLDWDEGPFYQSERF
PVYREFVERLLAEGKAYRCYCTPEELEARREQAMKEGRKPKYDGTCRDLVTQDADRPFVVRFRAPHEGVTAFDDLIKGRI
AFNNEELDDLIIQRTDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDEEIFSRQDLVEKFTIEAVGKSAGVFNPDKLLWLNHHYIKESSPERLA
ELLVPFLRERGVDPSTGPSLPQVVKTLQERSRTLVEMADGALFYYRSEISYDEEAAAKHLVPGTLPLLASLAEKLDACAD
FSHSGLEAAFKEFIAEKGIKFGQLGPAVRVSLCGGTASPGIYEVVEALGKDETLRRLRRAVAYLEQ

Sequences:

>Translated_466_residues
MSDVRLRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVARSTQESVDAILQGMEWLGLDWDEGPFYQSERF
PVYREFVERLLAEGKAYRCYCTPEELEARREQAMKEGRKPKYDGTCRDLVTQDADRPFVVRFRAPHEGVTAFDDLIKGRI
AFNNEELDDLIIQRTDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDEEIFSRQDLVEKFTIEAVGKSAGVFNPDKLLWLNHHYIKESSPERLA
ELLVPFLRERGVDPSTGPSLPQVVKTLQERSRTLVEMADGALFYYRSEISYDEEAAAKHLVPGTLPLLASLAEKLDACAD
FSHSGLEAAFKEFIAEKGIKFGQLGPAVRVSLCGGTASPGIYEVVEALGKDETLRRLRRAVAYLEQ
>Mature_465_residues
SDVRLRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVARSTQESVDAILQGMEWLGLDWDEGPFYQSERFP
VYREFVERLLAEGKAYRCYCTPEELEARREQAMKEGRKPKYDGTCRDLVTQDADRPFVVRFRAPHEGVTAFDDLIKGRIA
FNNEELDDLIIQRTDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSKR
HGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDEEIFSRQDLVEKFTIEAVGKSAGVFNPDKLLWLNHHYIKESSPERLAE
LLVPFLRERGVDPSTGPSLPQVVKTLQERSRTLVEMADGALFYYRSEISYDEEAAAKHLVPGTLPLLASLAEKLDACADF
SHSGLEAAFKEFIAEKGIKFGQLGPAVRVSLCGGTASPGIYEVVEALGKDETLRRLRRAVAYLEQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=30.4259634888438, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI62241042, Length=393, Percent_Identity=25.1908396946565, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=52.5862068965517, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=38.2470119521912, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786895, Length=347, Percent_Identity=22.7665706051873, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=31.8658280922432, Blast_Score=238, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=33.739837398374, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321192, Length=258, Percent_Identity=28.6821705426357, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=123, Percent_Identity=37.3983739837398, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=35.655737704918, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24649466, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_GEOSL (Q74DU6)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952272.1
- ProteinModelPortal:   Q74DU6
- SMR:   Q74DU6
- GeneID:   2688264
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1219
- NMPDR:   fig|243231.1.peg.1210
- TIGR:   GSU1219
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   GSUL243231:GSU_1219-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52402; Mature: 52271

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVRLRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVARSTQESVDAILQ
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMEWLGLDWDEGPFYQSERFPVYREFVERLLAEGKAYRCYCTPEELEARREQAMKEGRKP
CHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCC
KYDGTCRDLVTQDADRPFVVRFRAPHEGVTAFDDLIKGRIAFNNEELDDLIIQRTDGTPT
CCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEECCCCHHHHHEECCCCCCC
YNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
EEEEEEECCCCCCEEEEEECCCCCCCCHHHEEEEHHHCCCCCCHHCCCEEECCCHHHHHH
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDEEIFSRQDLVEKFTIEAVGKSAGVFNP
HCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKLLWLNHHYIKESSPERLAELLVPFLRERGVDPSTGPSLPQVVKTLQERSRTLVEMADG
CCEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALFYYRSEISYDEEAAAKHLVPGTLPLLASLAEKLDACADFSHSGLEAAFKEFIAEKGIK
EEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
FGQLGPAVRVSLCGGTASPGIYEVVEALGKDETLRRLRRAVAYLEQ
CCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVRLRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVARSTQESVDAILQ
CCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMEWLGLDWDEGPFYQSERFPVYREFVERLLAEGKAYRCYCTPEELEARREQAMKEGRKP
CHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCC
KYDGTCRDLVTQDADRPFVVRFRAPHEGVTAFDDLIKGRIAFNNEELDDLIIQRTDGTPT
CCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEECCCCHHHHHEECCCCCCC
YNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
EEEEEEECCCCCCEEEEEECCCCCCCCHHHEEEEHHHCCCCCCHHCCCEEECCCHHHHHH
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDEEIFSRQDLVEKFTIEAVGKSAGVFNP
HCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKLLWLNHHYIKESSPERLAELLVPFLRERGVDPSTGPSLPQVVKTLQERSRTLVEMADG
CCEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALFYYRSEISYDEEAAAKHLVPGTLPLLASLAEKLDACADFSHSGLEAAFKEFIAEKGIK
EEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
FGQLGPAVRVSLCGGTASPGIYEVVEALGKDETLRRLRRAVAYLEQ
CCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA