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The map label for this gene is yibK [C]

Identifier: 39996299

GI number: 39996299

Start: 1300548

End: 1301042

Strand: Reverse

Name: yibK [C]

Synonym: GSU1197

Alternate gene names: 39996299

Gene position: 1301042-1300548 (Counterclockwise)

Preceding gene: 39996311

Following gene: 39996298

Centisome position: 34.11

GC content: 62.63

Gene sequence:

>495_bases
ATGACTGGACACCAGCCCTTTCACATTGTGCTCGTGGAGCCGGAAATCCCACCCAACACCGGCAACATTGCCCGCCTTTG
CGGGGCCACCGGCACCGTACTCCACCTGGTCGGAAAACTTGGCTTTTCTACCGATGACCGGTACCTGAAGAGAGCCGGCC
TCGACTACTGGAGCGAGGTGGATATCCGCTACTGGGAGAGCCTCGACCAACTGCGGCAGGCCTGGCCCGACGCGCGCTTC
TTCTATACGAGCAAGAAGGCGGCGCGCTGTTACGTGGATGCCGGCTTTCGCGAGGGCGACTTCATCGTCTTCGGCAAGGA
AACCGTTGGACTGCCCGAAGACCTGCTCCGGGCCAACGAGGAGCACTGCGTGAGGATACCGATCTTCGGCAAGGTGCGGA
GCCTCAACCTCTCAACCGCCGCGGGGATCGTCCTGTACGAAGCCCTGCGCCAGACCGGACGCCTTGCCGACCGGGCGGGT
GAAAACGGAGAGTGA

Upstream 100 bases:

>100_bases
GCGGAGAAGCGCTAATAATAGACCCCCCGATTGAAAATTGCAAGACCGGCGAACCCTCCTGCCGGTATACGAGGGGGGAG
CGTTCCCGCGGGAAGGACCC

Downstream 100 bases:

>100_bases
TGTCGATGATCAAGGCCGGAATCACGTTCATGGTGCTGGCTCTCGTCTTCTACACCTACGCGGTCTTCAGCGGCAGGAAA
GAGGGACTCCACGCCAAGCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTGHQPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVDIRYWESLDQLRQAWPDARF
FYTSKKAARCYVDAGFREGDFIVFGKETVGLPEDLLRANEEHCVRIPIFGKVRSLNLSTAAGIVLYEALRQTGRLADRAG
ENGE

Sequences:

>Translated_164_residues
MTGHQPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVDIRYWESLDQLRQAWPDARF
FYTSKKAARCYVDAGFREGDFIVFGKETVGLPEDLLRANEEHCVRIPIFGKVRSLNLSTAAGIVLYEALRQTGRLADRAG
ENGE
>Mature_163_residues
TGHQPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVDIRYWESLDQLRQAWPDARFF
YTSKKAARCYVDAGFREGDFIVFGKETVGLPEDLLRANEEHCVRIPIFGKVRSLNLSTAAGIVLYEALRQTGRLADRAGE
NGE

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=43.2432432432432, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18401; Mature: 18270

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHQPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHC
DIRYWESLDQLRQAWPDARFFYTSKKAARCYVDAGFREGDFIVFGKETVGLPEDLLRANE
HHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHCCCC
EHCVRIPIFGKVRSLNLSTAAGIVLYEALRQTGRLADRAGENGE
CCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TGHQPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHC
DIRYWESLDQLRQAWPDARFFYTSKKAARCYVDAGFREGDFIVFGKETVGLPEDLLRANE
HHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHCCCC
EHCVRIPIFGKVRSLNLSTAAGIVLYEALRQTGRLADRAGENGE
CCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]