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The map label for this gene is mdeA [H]

Identifier: 39996285

GI number: 39996285

Start: 1290337

End: 1291623

Strand: Direct

Name: mdeA [H]

Synonym: GSU1183

Alternate gene names: 39996285

Gene position: 1290337-1291623 (Clockwise)

Preceding gene: 39996284

Following gene: 39996286

Centisome position: 33.83

GC content: 60.61

Gene sequence:

>1287_bases
ATGTCCGACATCCAAAAAGGCTTCGATACCCTCGCACTCCACGCCGGCCAGGATCCGGATCCCACGACGCTGTCGCGTGC
GGTTCCCATTTACCAGACCTCTTCCTATGCGTTCCGCAGCTCCGAACACGCGGCCAATCTCTTTGGTCTCCGGGAGTTCG
GCAATATCTACACGCGGATCATGAATCCGACCTGCGATGTACTGGAAAAGCGCCTGGCGGAACTTGACGGCGGAGTGGGC
GCGCTTGCTCTCGCCTCGGGGCAGGCGGCAATCACGTATGCGGTGCTTAACATCGCCGGCGCCGGGCAGAATATCGTCTC
CACCAGCTATCTCTATGGCGGAACCTACAACCTCTTCCACTATACTCTGCCAAGATTGGGGATATCGGTCCGGTTCGTCG
ACACTTCTGACCCTGAAAACGTCCGTCGGGCCATGGATGAAAACACTCGCCTGGTCTACACCGAATCGGTAGGGAACCCG
AAAAACAATGTGGACGACTTCGAGTCCATTGCCCGGATCGCCCATGAGGCGGGAATCCCGTTCATAGTGGACAACACCGT
TACCACTCCGTACCTGTTCAGGCCTTTTGACCATGGGGCCGACATCGCCGTCTATTCCCTCACCAAATTCATCGGTGGCC
ACGGTACGAGCATCGGGGGGGCGGTGGTAGACAGCGGACGTTTTCCCTGGAACAACGGCCGGTTCCCCGAGTTCACGGAA
CCGGATCCCTCCTACCATGGTTTGCGCTACTGGGAGGCCCTGGGGAACCTCTCCTACATCCTCAAGATGCGGATCACGCT
CCTGCGCGATATGGGGGCCTGCCTCGCGCCGTTCAACGCATTCCTCTTCCTCCAGGGGCTGGAGACCTTGCCGGTGCGCA
TGGCACGCCACGTTGACAACGCGCGTACTGTTGCCGAGTGGCTGGAGCGGCATCCACTGGTCACCTGGGTCAACTATCCG
GGCCTGCCCAGCCACCGGGACCACGACAATGCCGGCAAGTACCTCCCCAAGGGCGCCGGTGCCATCATCGGCTTCGGAGT
CAAGGGAGGGCTCGAGGCGGGCAAGAAGTTCATCGACAGCGTGAAGCTCCTGTCGCATCTTGCCAACATCGGCGACGCCA
AGTCCCTCGTCATCCACCCGGCATCCACCACGCACGAGCAGCTCACCGATGAAGAGCGTCTCTCGGCCGGGGTAACGCCG
GATTTCATCCGCCTTTCCGTCGGCATCGAGGATGTGGCCGACATCATTGCCGACATCGACCAGGCCCTGCATGCCTCCCA
ATCCTGA

Upstream 100 bases:

>100_bases
TCTTTTGTTGACATGTATGGTATAGTATATGTATTATGTGATGACGGGTAACTGTACTTTTGTTGTAGGGTAGCATCACA
TACGAGAGGAGAGTTCCATC

Downstream 100 bases:

>100_bases
GCCCACGTGCGCAACTTCGCCCCGGGACTGCCTTCGGGCCGCCCCTGGGGTGCCGCCGGCTTTTCCCCCTGTGGACGGGG
CTCCGTGAACATCCTTGCCC

Product: O-acetyl-L-homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRIMNPTCDVLEKRLAELDGGVG
ALALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFHYTLPRLGISVRFVDTSDPENVRRAMDENTRLVYTESVGNP
KNNVDDFESIARIAHEAGIPFIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFTE
PDPSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVRMARHVDNARTVAEWLERHPLVTWVNYP
GLPSHRDHDNAGKYLPKGAGAIIGFGVKGGLEAGKKFIDSVKLLSHLANIGDAKSLVIHPASTTHEQLTDEERLSAGVTP
DFIRLSVGIEDVADIIADIDQALHASQS

Sequences:

>Translated_428_residues
MSDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRIMNPTCDVLEKRLAELDGGVG
ALALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFHYTLPRLGISVRFVDTSDPENVRRAMDENTRLVYTESVGNP
KNNVDDFESIARIAHEAGIPFIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFTE
PDPSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVRMARHVDNARTVAEWLERHPLVTWVNYP
GLPSHRDHDNAGKYLPKGAGAIIGFGVKGGLEAGKKFIDSVKLLSHLANIGDAKSLVIHPASTTHEQLTDEERLSAGVTP
DFIRLSVGIEDVADIIADIDQALHASQS
>Mature_427_residues
SDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRIMNPTCDVLEKRLAELDGGVGA
LALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFHYTLPRLGISVRFVDTSDPENVRRAMDENTRLVYTESVGNPK
NNVDDFESIARIAHEAGIPFIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFTEP
DPSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVRMARHVDNARTVAEWLERHPLVTWVNYPG
LPSHRDHDNAGKYLPKGAGAIIGFGVKGGLEAGKKFIDSVKLLSHLANIGDAKSLVIHPASTTHEQLTDEERLSAGVTPD
FIRLSVGIEDVADIIADIDQALHASQS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=35.1543942992874, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=32.0665083135392, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.952380952381, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=31.1004784688995, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=27.4418604651163, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=406, Percent_Identity=33.2512315270936, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=419, Percent_Identity=34.3675417661098, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=35.3221957040573, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=53.3642691415313, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=33.5697399527187, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=25.5656108597285, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=199, Percent_Identity=30.6532663316583, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=426, Percent_Identity=24.8826291079812, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=33.4905660377358, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46736; Mature: 46605

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRI
CCHHHHHHHHEEEECCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
MNPTCDVLEKRLAELDGGVGALALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHCCCCCCHHEEHEEECCCHHHHH
YTLPRLGISVRFVDTSDPENVRRAMDENTRLVYTESVGNPKNNVDDFESIARIAHEAGIP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFTE
EEECCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
PDPSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVRMARHVDN
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCH
ARTVAEWLERHPLVTWVNYPGLPSHRDHDNAGKYLPKGAGAIIGFGVKGGLEAGKKFIDS
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
VKLLSHLANIGDAKSLVIHPASTTHEQLTDEERLSAGVTPDFIRLSVGIEDVADIIADID
HHHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHH
QALHASQS
HHHHCCCC
>Mature Secondary Structure 
SDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRI
CHHHHHHHHEEEECCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
MNPTCDVLEKRLAELDGGVGALALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHCCCCCCHHEEHEEECCCHHHHH
YTLPRLGISVRFVDTSDPENVRRAMDENTRLVYTESVGNPKNNVDDFESIARIAHEAGIP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFTE
EEECCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
PDPSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVRMARHVDN
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCH
ARTVAEWLERHPLVTWVNYPGLPSHRDHDNAGKYLPKGAGAIIGFGVKGGLEAGKKFIDS
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
VKLLSHLANIGDAKSLVIHPASTTHEQLTDEERLSAGVTPDFIRLSVGIEDVADIIADID
HHHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHH
QALHASQS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]