Definition Geobacter sulfurreducens PCA chromosome, complete genome.
Accession NC_002939
Length 3,814,139

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The map label for this gene is asnS

Identifier: 39996258

GI number: 39996258

Start: 1261648

End: 1263033

Strand: Direct

Name: asnS

Synonym: GSU1156

Alternate gene names: 39996258

Gene position: 1261648-1263033 (Clockwise)

Preceding gene: 39996254

Following gene: 39996259

Centisome position: 33.08

GC content: 63.56

Gene sequence:

>1386_bases
ATGAACGAGCGGATACGGATACGGGAGATACTGGCCGGAACACCAGCCGGTACGGAAGTGATCGTCAAGGGGTGGGTCAG
GACGAGTCGCGTCGGCAAGGGGGTTGCCTTTCTTGCGGTGAATGACGGATCGTGTCTGGCGAGTCTCCAGGTGGTGGCCG
AACCGGGACTCGCCAACTACCATGAGCTGCGGGGCATCGGCACCGGCGCCGCCGTGGCGGTCCGGGGGATCGTTGCCGAA
TCGCCGGCCGCGGGGCAGGCCGTCGAACTCCACGCGACGAAGGTGGTCGTGGTCGGAGGCGCCGACGAACAGTATCCCCT
GCAGAAGAAGCGCCACACCTTCGAGTACCTGCGGACCATTGCCCACTTGCGTCCCCGCTCCAACACCTTCGGCGCGGTGT
TCCGCGTCCGCTCGTCGCTGGCCCAGGCTGTTCACCGTTTCTTTGCCGAACGGGGCTTTCTCTACGTCCATACCCCTATC
ATCACCACCAGCGACTGCGAAGGGGCAGGCGAACTGTTCCGGGTCACCACTCTCGATCCGTCGGCCCCACCCATGGCGGA
CGGCGCGGTCGATTTCAGCCAGGATTTCTTCGCGGCCCAGGCGGGGCTCACCGTCAGCGGCCAGCTTGAGGGTGAACTCT
TTGCCCAGGCATTTTCCGATATTTACACCTTCGGGCCTACCTTCAGGGCTGAAAATTCCAACACTCCCCGCCATGCCGCC
GAATTCTGGATGATCGAGCCCGAAATGGCCTTTGCCGACCTGAGGGACGATGCCGCGCTGGCCGAGGATTTTTTCCGTTA
CCTGTGTCGCCACGTTCTGGACAACTGCGCCGAGGACATGGCCTTTTTCAACGAACACGTGGACCGTGGGCTACTCGCCC
GGGTAGAGCAGGTGGCCGGTTCATCCTTTGCCATGATGGAGTACGGCGTGGCCATCGAGCACCTGAAGCGGGCCGCGGTC
CCCTTCGAGTACCCGGTCGAGTGGGGTCTCGACCTCCAGACCGAGCATGAGCGTTACCTGACCGAGCAGGTGGTGGGCGG
GCCGGTCTTCGTGGTCAACTATCCTCAGGAGATCAAGGCCTTCTACATGCGCCGGAATGACGACGGCAGAACCGTGGCGG
CCATGGATCTGCTGGTCCCCAAGGTGGGCGAGATCATCGGTGGCAGCCAGCGGGAGGAGCGGTACGATCTTCTGGAATCC
CGCATGCGCGAGGGCGGCATTGCTCCCGAGAGCCTCTGGTGGTACCTGGACTCCCGCCGCTGGGGCTCGACTCCCCACGC
CGGTTTCGGCCTCGGTTTCGAGCGTCTCATTATGTACCTGACCGGCATGGAGAATATCCGCGACGTGATCCCGTTCCCCC
GTACCCCGCGCCACGCGGAGTTTTGA

Upstream 100 bases:

>100_bases
TTACGCATGAATCTCTTCTCCTTTGCTGTACAATTGGCTTCGTCGTTCCTCCAGACTATACCGAAGGGGAAGGGCAAGTC
AAATGGGTAGGGGACACGCC

Downstream 100 bases:

>100_bases
GAATCATAGCATAGTACGTTCCGGAGCTCGCACCATGTTCGTTCACTCGCTCTGTCTTCACCTTCACCTGCCGAGCCATT
CCCTCAAGGGGAAGCGGGGG

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNERIRIREILAGTPAGTEVIVKGWVRTSRVGKGVAFLAVNDGSCLASLQVVAEPGLANYHELRGIGTGAAVAVRGIVAE
SPAAGQAVELHATKVVVVGGADEQYPLQKKRHTFEYLRTIAHLRPRSNTFGAVFRVRSSLAQAVHRFFAERGFLYVHTPI
ITTSDCEGAGELFRVTTLDPSAPPMADGAVDFSQDFFAAQAGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTPRHAA
EFWMIEPEMAFADLRDDAALAEDFFRYLCRHVLDNCAEDMAFFNEHVDRGLLARVEQVAGSSFAMMEYGVAIEHLKRAAV
PFEYPVEWGLDLQTEHERYLTEQVVGGPVFVVNYPQEIKAFYMRRNDDGRTVAAMDLLVPKVGEIIGGSQREERYDLLES
RMREGGIAPESLWWYLDSRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF

Sequences:

>Translated_461_residues
MNERIRIREILAGTPAGTEVIVKGWVRTSRVGKGVAFLAVNDGSCLASLQVVAEPGLANYHELRGIGTGAAVAVRGIVAE
SPAAGQAVELHATKVVVVGGADEQYPLQKKRHTFEYLRTIAHLRPRSNTFGAVFRVRSSLAQAVHRFFAERGFLYVHTPI
ITTSDCEGAGELFRVTTLDPSAPPMADGAVDFSQDFFAAQAGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTPRHAA
EFWMIEPEMAFADLRDDAALAEDFFRYLCRHVLDNCAEDMAFFNEHVDRGLLARVEQVAGSSFAMMEYGVAIEHLKRAAV
PFEYPVEWGLDLQTEHERYLTEQVVGGPVFVVNYPQEIKAFYMRRNDDGRTVAAMDLLVPKVGEIIGGSQREERYDLLES
RMREGGIAPESLWWYLDSRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
>Mature_461_residues
MNERIRIREILAGTPAGTEVIVKGWVRTSRVGKGVAFLAVNDGSCLASLQVVAEPGLANYHELRGIGTGAAVAVRGIVAE
SPAAGQAVELHATKVVVVGGADEQYPLQKKRHTFEYLRTIAHLRPRSNTFGAVFRVRSSLAQAVHRFFAERGFLYVHTPI
ITTSDCEGAGELFRVTTLDPSAPPMADGAVDFSQDFFAAQAGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTPRHAA
EFWMIEPEMAFADLRDDAALAEDFFRYLCRHVLDNCAEDMAFFNEHVDRGLLARVEQVAGSSFAMMEYGVAIEHLKRAAV
PFEYPVEWGLDLQTEHERYLTEQVVGGPVFVVNYPQEIKAFYMRRNDDGRTVAAMDLLVPKVGEIIGGSQREERYDLLES
RMREGGIAPESLWWYLDSRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=459, Percent_Identity=46.6230936819172, Blast_Score=410, Evalue=1e-115,
Organism=Homo sapiens, GI4758762, Length=452, Percent_Identity=30.3097345132743, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI45439306, Length=492, Percent_Identity=27.0325203252033, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787161, Length=464, Percent_Identity=56.8965517241379, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17556372, Length=430, Percent_Identity=30.6976744186047, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71984122, Length=452, Percent_Identity=30.5309734513274, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17551876, Length=494, Percent_Identity=24.6963562753036, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319872, Length=466, Percent_Identity=37.5536480686695, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=464, Percent_Identity=28.8793103448276, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=504, Percent_Identity=26.1904761904762, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922416, Length=463, Percent_Identity=35.2051835853132, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI19921528, Length=464, Percent_Identity=29.0948275862069, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI17136276, Length=484, Percent_Identity=25, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_GEOSL (Q74E08)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952209.1
- ProteinModelPortal:   Q74E08
- SMR:   Q74E08
- GeneID:   2685936
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1156
- NMPDR:   fig|243231.1.peg.1147
- TIGR:   GSU1156
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   GSUL243231:GSU_1156-MONOMER
- BRENDA:   6.1.1.22
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 51307; Mature: 51307

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNERIRIREILAGTPAGTEVIVKGWVRTSRVGKGVAFLAVNDGSCLASLQVVAEPGLANY
CCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHEEECCCCCCH
HELRGIGTGAAVAVRGIVAESPAAGQAVELHATKVVVVGGADEQYPLQKKRHTFEYLRTI
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
AHLRPRSNTFGAVFRVRSSLAQAVHRFFAERGFLYVHTPIITTSDCEGAGELFRVTTLDP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEECCCCCCCCEEEEEECCC
SAPPMADGAVDFSQDFFAAQAGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTPRHAA
CCCCCCCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHH
EFWMIEPEMAFADLRDDAALAEDFFRYLCRHVLDNCAEDMAFFNEHVDRGLLARVEQVAG
HEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSFAMMEYGVAIEHLKRAAVPFEYPVEWGLDLQTEHERYLTEQVVGGPVFVVNYPQEIKA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHH
FYMRRNDDGRTVAAMDLLVPKVGEIIGGSQREERYDLLESRMREGGIAPESLWWYLDSRR
HHHEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHEEEEECCC
WGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNERIRIREILAGTPAGTEVIVKGWVRTSRVGKGVAFLAVNDGSCLASLQVVAEPGLANY
CCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHEEECCCCCCH
HELRGIGTGAAVAVRGIVAESPAAGQAVELHATKVVVVGGADEQYPLQKKRHTFEYLRTI
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
AHLRPRSNTFGAVFRVRSSLAQAVHRFFAERGFLYVHTPIITTSDCEGAGELFRVTTLDP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEECCCCCCCCEEEEEECCC
SAPPMADGAVDFSQDFFAAQAGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTPRHAA
CCCCCCCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHH
EFWMIEPEMAFADLRDDAALAEDFFRYLCRHVLDNCAEDMAFFNEHVDRGLLARVEQVAG
HEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSFAMMEYGVAIEHLKRAAVPFEYPVEWGLDLQTEHERYLTEQVVGGPVFVVNYPQEIKA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHH
FYMRRNDDGRTVAAMDLLVPKVGEIIGGSQREERYDLLESRMREGGIAPESLWWYLDSRR
HHHEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHEEEEECCC
WGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA