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The map label for this gene is ruvC [H]

Identifier: 39996177

GI number: 39996177

Start: 1163943

End: 1164437

Strand: Direct

Name: ruvC [H]

Synonym: GSU1075

Alternate gene names: 39996177

Gene position: 1163943-1164437 (Clockwise)

Preceding gene: 39996176

Following gene: 39996178

Centisome position: 30.52

GC content: 62.02

Gene sequence:

>495_bases
ATGAGAGTTCTCGGCATCGACCCCGGTTCACGCATCACCGGCTACGGCATTATCGAGAAGATCGGCAACCGTCTCGTGCA
CGTGGACAACGGCGCCATTCATACCGATAGCCACAGGGAGTTCGCGCTCCGACTCCACAAAATCTACGAGGGGCTGAGCC
GCGTCATCGAAGAGTACCGCCCTGATGCGATGGCTGTAGAGCAGGTGTTCCTCGCCCATAACGCCCAGAGCGCCCTGAAG
CTGGGCCAGGCCCGCGGGGCTGCCATCGTGGCCGGAGTAAGCGCGGGATTGCCGGTCAGCGAGTATACGGCCATGCAGGT
AAAGCAGGCGGTGGTCGGCTATGGCCACGCCCGCAAGGAGCAGGTGCAGCAGATGGTCAAATCGCTCCTCAATCTCCCCG
AGATCGCCCAGGCCGATGCCTCCGATGCCTTGGCCGTAGCGGTCTGCCACGCCAATTCAGCAGTGATGAAATCCGTGCTC
CGGAGCATCCGATGA

Upstream 100 bases:

>100_bases
CCCCCGGTTGGTTCAAGCGCGGAAGTCGGAAGCCGGGTCTTTCCTTTCGCGCCTTTTTTCTGCTGTGCTCTCCGCGCCCT
CTGTGGCAGAATGTCTCCCC

Downstream 100 bases:

>100_bases
TCGCCCTTCTCACCGGCAGGCTCGCCCATAAATCCCCCGATGCCATCATTATCGATGTGAACGGTGTCGGCTACCGGGTC
CAGATTCCTTTCTCAACCTA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRVLGIDPGSRITGYGIIEKIGNRLVHVDNGAIHTDSHREFALRLHKIYEGLSRVIEEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVSAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAVMKSVL
RSIR

Sequences:

>Translated_164_residues
MRVLGIDPGSRITGYGIIEKIGNRLVHVDNGAIHTDSHREFALRLHKIYEGLSRVIEEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVSAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAVMKSVL
RSIR
>Mature_164_residues
MRVLGIDPGSRITGYGIIEKIGNRLVHVDNGAIHTDSHREFALRLHKIYEGLSRVIEEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVSAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAVMKSVL
RSIR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=157, Percent_Identity=54.7770700636943, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17679; Mature: 17679

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLGIDPGSRITGYGIIEKIGNRLVHVDNGAIHTDSHREFALRLHKIYEGLSRVIEEYR
CEEECCCCCCCCHHHHHHHHHCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHC
PDAMAVEQVFLAHNAQSALKLGQARGAAIVAGVSAGLPVSEYTAMQVKQAVVGYGHARKE
CCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
QVQQMVKSLLNLPEIAQADASDALAVAVCHANSAVMKSVLRSIR
HHHHHHHHHHCCCHHHHCCCCCHHEEHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRVLGIDPGSRITGYGIIEKIGNRLVHVDNGAIHTDSHREFALRLHKIYEGLSRVIEEYR
CEEECCCCCCCCHHHHHHHHHCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHC
PDAMAVEQVFLAHNAQSALKLGQARGAAIVAGVSAGLPVSEYTAMQVKQAVVGYGHARKE
CCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
QVQQMVKSLLNLPEIAQADASDALAVAVCHANSAVMKSVLRSIR
HHHHHHHHHHCCCHHHHCCCCCHHEEHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA