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The map label for this gene is 39996130

Identifier: 39996130

GI number: 39996130

Start: 1105733

End: 1106617

Strand: Reverse

Name: 39996130

Synonym: GSU1027

Alternate gene names: NA

Gene position: 1106617-1105733 (Counterclockwise)

Preceding gene: 39996131

Following gene: 39996129

Centisome position: 29.01

GC content: 64.86

Gene sequence:

>885_bases
ATGTCCAAACTGAGCGTCGCCCTTGTCCAGCAGAGCTGCACCGCCGACAAGGATCTGAACCTGGCCAAAAGCATCGAGAA
CATCCGCAAGGCGAGCGTCCTGGGCGCAAAGCTCGTGGTGCTCCAGGAGCTGCACACGGGTCCCTATTTCTGCCAGAACG
AGGATACCGCCCATTTCGACCTGGCCGAGCCGATCCCCGGCCCCACCACGGAACTGCTCGGCGGCGTGGCCAAGGAATTC
GGCGTGGTGCTCGTTTCCTCGCTGTTCGAGCGCCGGGCGCCGGGGCTTTACCACAACACCGCCGTGGTCTTCGAAAAAGA
CGGCTCCATGGCCGGCACCTACCGCAAGATGCACATTCCCGACGACCCGGGCTATTACGAGAAGTTCTACTTCACCCCCG
GCGACCTTGGCTTCGAGCCGATCCGGACCTCGGTGGGAAAACTAGGGGTCCTGGTCTGCTGGGATCAGTGGTATCCCGAA
GCCGCCCGGCTCATGGCCCTGGCTGGCGCCGACCTCCTCATCTATCCCACGGCCATCGGCTGGGACCCCCGTGACGACGA
CGACGAGAAGATCCGCCAGAAGGAAGCCTGGATCACCATCCAACGGGGCCATGCCGTGGCCAACGGCATCCCGGTGGTGA
GCGTCAACCGCGTGGGACACGAGTCGGATCCCTCGGGAGTCCTCCCCGGCAGCCAGTTCTGGGGTTCGAGCTTCGTGGCC
GGCCCCCAGGGCGAGATCCTGGCCCAGGCTTCCAACGACGGGGAGGAATTGCTCATCACGGAACTGGACTTGGCCCGTAG
CGAGGCGGTCCGTCGCATCTGGCCGTTCCTGCGTGACCGGCGCATTGACGCCTACGGCGACCTGCTGCGCCGCTACCGAG
ACTGA

Upstream 100 bases:

>100_bases
TTCCGGGCCGCGAGATCGTCGGCATCGACTGCCGCCCCCTCATCATCCAGCACGGTTCGCTCCACTGCGTAACCATGCAG
TTGCCCAAAGGAGTGCTTCC

Downstream 100 bases:

>100_bases
GAGGCCCCCATGCTCCAGGACAAGGAACTGCTCGAAGCCCTCTCCAGCGCCCGGGTCCTCACCTCCATGATCACGCCGGC
GGTGCTTATTTCAGCCGCCG

Product: glycosy hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSKLSVALVQQSCTADKDLNLAKSIENIRKASVLGAKLVVLQELHTGPYFCQNEDTAHFDLAEPIPGPTTELLGGVAKEF
GVVLVSSLFERRAPGLYHNTAVVFEKDGSMAGTYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDDDDEKIRQKEAWITIQRGHAVANGIPVVSVNRVGHESDPSGVLPGSQFWGSSFVA
GPQGEILAQASNDGEELLITELDLARSEAVRRIWPFLRDRRIDAYGDLLRRYRD

Sequences:

>Translated_294_residues
MSKLSVALVQQSCTADKDLNLAKSIENIRKASVLGAKLVVLQELHTGPYFCQNEDTAHFDLAEPIPGPTTELLGGVAKEF
GVVLVSSLFERRAPGLYHNTAVVFEKDGSMAGTYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDDDDEKIRQKEAWITIQRGHAVANGIPVVSVNRVGHESDPSGVLPGSQFWGSSFVA
GPQGEILAQASNDGEELLITELDLARSEAVRRIWPFLRDRRIDAYGDLLRRYRD
>Mature_293_residues
SKLSVALVQQSCTADKDLNLAKSIENIRKASVLGAKLVVLQELHTGPYFCQNEDTAHFDLAEPIPGPTTELLGGVAKEFG
VVLVSSLFERRAPGLYHNTAVVFEKDGSMAGTYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEA
ARLMALAGADLLIYPTAIGWDPRDDDDEKIRQKEAWITIQRGHAVANGIPVVSVNRVGHESDPSGVLPGSQFWGSSFVAG
PQGEILAQASNDGEELLITELDLARSEAVRRIWPFLRDRRIDAYGDLLRRYRD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=297, Percent_Identity=29.2929292929293, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI7706509, Length=276, Percent_Identity=29.3478260869565, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI297632348, Length=295, Percent_Identity=30.5084745762712, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI5031947, Length=295, Percent_Identity=30.5084745762712, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI297632350, Length=295, Percent_Identity=30.5084745762712, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=276, Percent_Identity=30.7971014492754, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=25, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=30.8724832214765, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322335, Length=317, Percent_Identity=27.1293375394322, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21358471, Length=282, Percent_Identity=32.2695035460993, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17933642, Length=287, Percent_Identity=26.4808362369338, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355835, Length=299, Percent_Identity=27.4247491638796, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32563; Mature: 32432

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00591 GLYCOSYL_HYDROL_F10 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLSVALVQQSCTADKDLNLAKSIENIRKASVLGAKLVVLQELHTGPYFCQNEDTAHFD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEE
LAEPIPGPTTELLGGVAKEFGVVLVSSLFERRAPGLYHNTAVVFEKDGSMAGTYRKMHIP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCEEEEECC
DDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDDDDEKIRQKEAWITIQRGHAVANGIPVVSVNRVGHESDPSGVLPGSQFWGSSFVA
CCCCCCCHHHHHHHHHEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCC
GPQGEILAQASNDGEELLITELDLARSEAVRRIWPFLRDRRIDAYGDLLRRYRD
CCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SKLSVALVQQSCTADKDLNLAKSIENIRKASVLGAKLVVLQELHTGPYFCQNEDTAHFD
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEE
LAEPIPGPTTELLGGVAKEFGVVLVSSLFERRAPGLYHNTAVVFEKDGSMAGTYRKMHIP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCEEEEECC
DDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDDDDEKIRQKEAWITIQRGHAVANGIPVVSVNRVGHESDPSGVLPGSQFWGSSFVA
CCCCCCCHHHHHHHHHEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCC
GPQGEILAQASNDGEELLITELDLARSEAVRRIWPFLRDRRIDAYGDLLRRYRD
CCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]