Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 39996074

GI number: 39996074

Start: 1047989

End: 1048312

Strand: Reverse

Name: prfA [H]

Synonym: GSU0971

Alternate gene names: 39996074

Gene position: 1048312-1047989 (Counterclockwise)

Preceding gene: 39996096

Following gene: 39996064

Centisome position: 27.48

GC content: 59.57

Gene sequence:

>324_bases
ATGACGGTGGACATCAGGGACGCGGATATCAAGGTGGAGTACTATCGTGCTTCAGGACCCGGCGGGCAGCACCGTAATAC
CACCGATTCGGCGGTGCGCATCCGCCACCTTCCCACCGGAATCGTGGTCCAGGCCAGCGAAAGCCGCTCTCAGGCGCAGA
ATCGCGAGAAGGCCATGGAGCGTCTGCGGATTGCGCTGGAAAAGCGTGAACGTAAGGCGAAAAAGCGGATCGCCACCACG
GTGCCCAGAGGCGCCAGGGAAAAGCGACTGACGGAGAAGAAGCTGGTAGGGATGAAAAAAAAGCTGCGCTCAACGGTTGA
GTGA

Upstream 100 bases:

>100_bases
TTAACCATTTTTTTTAGGCAGGCCGGGTGGTTGCAACCGGTGGCAAAGCTTGCGCGTTTCATGCTACCCTGGCAACCGGA
ATCAAAGGAGGGATGCTGCG

Downstream 100 bases:

>100_bases
CCGCCGAGCGCAGCGAAGCTGCCGTTGCCGCGGGACTGCCGATTATCTTCCCGTCACCACGACACGACGCCCAGCATCTT
GCGTAAGACAACCAGCACGT

Product: peptidyl-tRNA hydrolase domain-containing protein

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MTVDIRDADIKVEYYRASGPGGQHRNTTDSAVRIRHLPTGIVVQASESRSQAQNREKAMERLRIALEKRERKAKKRIATT
VPRGAREKRLTEKKLVGMKKKLRSTVE

Sequences:

>Translated_107_residues
MTVDIRDADIKVEYYRASGPGGQHRNTTDSAVRIRHLPTGIVVQASESRSQAQNREKAMERLRIALEKRERKAKKRIATT
VPRGAREKRLTEKKLVGMKKKLRSTVE
>Mature_106_residues
TVDIRDADIKVEYYRASGPGGQHRNTTDSAVRIRHLPTGIVVQASESRSQAQNREKAMERLRIALEKRERKAKKRIATTV
PRGAREKRLTEKKLVGMKKKLRSTVE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=62, Percent_Identity=56.4516129032258, Blast_Score=74, Evalue=2e-14,
Organism=Homo sapiens, GI34577120, Length=55, Percent_Identity=52.7272727272727, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787462, Length=59, Percent_Identity=57.6271186440678, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI2367172, Length=108, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321295, Length=59, Percent_Identity=54.2372881355932, Blast_Score=70, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921226, Length=63, Percent_Identity=63.4920634920635, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 12239; Mature: 12108

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDIRDADIKVEYYRASGPGGQHRNTTDSAVRIRHLPTGIVVQASESRSQAQNREKAME
CCCCCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEECCCCEEEECCCHHHHHHHHHHHHH
RLRIALEKRERKAKKRIATTVPRGAREKRLTEKKLVGMKKKLRSTVE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVDIRDADIKVEYYRASGPGGQHRNTTDSAVRIRHLPTGIVVQASESRSQAQNREKAME
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEECCCCEEEECCCHHHHHHHHHHHHH
RLRIALEKRERKAKKRIATTVPRGAREKRLTEKKLVGMKKKLRSTVE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA