Click here to switch to the map view.

The map label for this gene is rne [H]

Identifier: 39996024

GI number: 39996024

Start: 986118

End: 988544

Strand: Reverse

Name: rne [H]

Synonym: GSU0921

Alternate gene names: 39996024

Gene position: 988544-986118 (Counterclockwise)

Preceding gene: 39996034

Following gene: 39996022

Centisome position: 25.92

GC content: 63.82

Gene sequence:

>2427_bases
ATGGCAAAAAAGATGCTCATCAACGTGATGCATCCGGAGGAATCACGCGTCGCCATCGTTGAAGACGGCAAGCTGGTTGA
TCTGGACATCGAAATCGAGGGGAGCGAACAAACCCGAGGCAACGTCTACAAGGGGGTGGTGGTCCGGGTGGAACCGGGAC
TGCAGGCCGCCTTCGTCGACATCGGACTGAAACGGCTCGGCTTCCTCCAGATGGGGGAAATCCATCCCTCGTTCTGGCAA
TGGCGCAGCGATATCCCCGAAGAGAGCCGCAGCCGCCGTCCCCGCATCCAGGAAGTCATCCGCCGGGGGCAGGAGCTCAT
TGTCCAGGTGGAAAAGGGCGAACGGGACATGAAGGGCGCGGCTCTGACCACCTACATGTCGTTTCCGGGCCGCTACATGG
TGCTCATGCCCGGCAGCGACTCGGCGGGCATTTCCCGCAAGGTGGAGAGCGAGTCCGACCGGAAGAAGCTCAAGGAAAAG
GTGGCCGAGCTGGAAATCCCCGAAGGCTACGGCTACATCGTCCGGACCGAGGCCCTGGGCAAGACCAAGACGGAACTGGC
CAAGGATCTCCAGTACCTGGTGAAGCTCCACCAGGGCATCGTGGACAAGGCAGCCGCGGCCAAGGGCCCCACTCTTCTCC
ACCGGGAACTGGACGTGGTCATCCGCACCATCCGCGACTACTTCACCGCCGAGATCGACGAAGTGCTGGTGGACAGCAAG
GACGTCTACAAGCAGGCCCGCGAATTCTTCAAGCAGACCATGCCCAAGTTCGAGAAGCTGGTGAAGCTCCACACGGAAAA
GCGCCCCATCTTCTCCCGCTACCAGATCGAAGAACAGATCGACCTCATCTACGAAAAGAAAGTTCCCCTCAAATCGGGCG
GCTACCTCATCATTGAGCCAACCGAGGCCCTGGTATCCATCGACGTCAACTCAGGCAAGACCACCGGCGAGAAGGGGGTC
GAGGACACCGCCTACAAGACCAATCTGGAAGCGGCCGAAGAGGCTGCCCGTCAGCTGCGGATGCGCGACCTGGGCGGGCT
CATCGTGCTCGACTTCATCGACATGCGGGACAAGAAGCACATCACCGCCGTGGAAAAGACCCTGAAGGCGGCCCTCAAGG
GTGACAAGGCCCGGGTCACGGTGGGGCGCATTTCCCAGTTCGGCATGCTGGAGATGTCGCGCCAGCGGATTCGCCAGACC
CTCGAACAGGGAAGCACCCTCGAATGCCCGCACTGCAGCGGGCGGGGCAAGGTGAAGAGCGTCGAGAACATGGCACTCTC
CTTCCTGCGCAAGGTCCACGCCGCAGCGGCCAAGGGGACCGTGGCCGAGGTGCACGGCGGGCTTCCCCTGGAGGTCGCCT
ACTACCTGCTGAACCGCAAGAAGAGGGAACTGGCCCAGATCGAGAACGAGTACGACATCGTCGTGACCGTGAAGGGCAAG
ACCTCGTTCCTGATGAACCAGCTGGAACTGGAGCTGGTCAGGCGCGAAAAGCCGGCCCACGTGGAAACACATGAAGAGCA
CGCGGAGAAGGTGGCAGAGGCGCTCGCCGAAGGGCTGCCCGAAGCAGCCGAAGGGACCGACGTCCCGGCCGAGGCATCGA
CAGCCGAGACCGAGGGCAAGAAAAAGAAACGGCGCCGCAGCCGCCGGAAGGGCAAGGCCGAAGCCGAAGAGATGGTAGAG
TCCGTAGCGGCTGCTGAAATCGAAGAACCCGAGGAGCTCGAAGCCGGCGGCGGAGAAGAACCGGAGGCCGCGGCTGGCGA
AGAAACGGCGGAAGGGGAGGCTCCGGGCGAAGAAGCCAGGAAGAAGCGGCGGCGCAAGCGGCGGCGCGGCAAGAAGGCAA
AAGCGGACGAAACCGCGGCCGAAGAAGCTTCAGCCCCGGAGATCGCCGAAGAGCCGGCAGAAGAGGAGACCACCGAAGCG
CCGATCCCTGCCGAAGAAGCAGGAGAAGCTTCGGAAGAGGTGAAGAAGAAGCGGCGGCGGCGCAAGCGCCGGACCCCAAA
ACGTGCCGGCGAGGCGACCGAGGCGCCGGCAACGGAAGTGGAGCCGGCTGAGCCCCGTGCCGCGGAGCCCGAACCGGAGC
TCGCCCCGGCCGAAGCGTCCCCAGAAAGCGCCGAGCCCGCTCCCAAAAAGACGCGCGCGCCCCGCAGCCGCAAAAAAGCG
GCCGAAAAGCCCGAGAAGACCGAGCCCGCCGTGGCTCTCCCCGAGGAACAGCAGGCGCCTGCCGCAGCTGAAGCGACCCC
GGTCACGCCGGCCGAAGCAAAACCGGCAAAAAAACGGGCCACGCGCAAAAAGACCACCTCCGAACCGGCACCCGCCCCGG
TACAGCCCCCTGTCCCGGAGCCTGCCGCTGACGAGCCGCCCAAGAAAAAACGGGCTCCGCGCAAAAAGAAAGAAGCCCCG
ACAGAAGGAACACCGGAGGCAGGATAG

Upstream 100 bases:

>100_bases
GGCGTTCGAATACCCATCGGGTCGCGCGGCCTGTGCCGCGGGCGACCATACATTTCCGCGGCGCGGGGCATTCCGCAGAC
CGCACAAAGGACTGAAATCA

Downstream 100 bases:

>100_bases
CAACTGAACACTCCGAAGCCGGCCTGTCCGGCCGCAGATCCCCACCTGGCAGGGCTTCACTCCGAAGCCCTGCCATTTCA
CATCCTACTGTATAGCTTTT

Product: ribonuclease, Rne/Rng family protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MAKKMLINVMHPEESRVAIVEDGKLVDLDIEIEGSEQTRGNVYKGVVVRVEPGLQAAFVDIGLKRLGFLQMGEIHPSFWQ
WRSDIPEESRSRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESESDRKKLKEK
VAELEIPEGYGYIVRTEALGKTKTELAKDLQYLVKLHQGIVDKAAAAKGPTLLHRELDVVIRTIRDYFTAEIDEVLVDSK
DVYKQAREFFKQTMPKFEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGV
EDTAYKTNLEAAEEAARQLRMRDLGGLIVLDFIDMRDKKHITAVEKTLKAALKGDKARVTVGRISQFGMLEMSRQRIRQT
LEQGSTLECPHCSGRGKVKSVENMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELAQIENEYDIVVTVKGK
TSFLMNQLELELVRREKPAHVETHEEHAEKVAEALAEGLPEAAEGTDVPAEASTAETEGKKKKRRRSRRKGKAEAEEMVE
SVAAAEIEEPEELEAGGGEEPEAAAGEETAEGEAPGEEARKKRRRKRRRGKKAKADETAAEEASAPEIAEEPAEEETTEA
PIPAEEAGEASEEVKKKRRRRKRRTPKRAGEATEAPATEVEPAEPRAAEPEPELAPAEASPESAEPAPKKTRAPRSRKKA
AEKPEKTEPAVALPEEQQAPAAAEATPVTPAEAKPAKKRATRKKTTSEPAPAPVQPPVPEPAADEPPKKKRAPRKKKEAP
TEGTPEAG

Sequences:

>Translated_808_residues
MAKKMLINVMHPEESRVAIVEDGKLVDLDIEIEGSEQTRGNVYKGVVVRVEPGLQAAFVDIGLKRLGFLQMGEIHPSFWQ
WRSDIPEESRSRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESESDRKKLKEK
VAELEIPEGYGYIVRTEALGKTKTELAKDLQYLVKLHQGIVDKAAAAKGPTLLHRELDVVIRTIRDYFTAEIDEVLVDSK
DVYKQAREFFKQTMPKFEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGV
EDTAYKTNLEAAEEAARQLRMRDLGGLIVLDFIDMRDKKHITAVEKTLKAALKGDKARVTVGRISQFGMLEMSRQRIRQT
LEQGSTLECPHCSGRGKVKSVENMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELAQIENEYDIVVTVKGK
TSFLMNQLELELVRREKPAHVETHEEHAEKVAEALAEGLPEAAEGTDVPAEASTAETEGKKKKRRRSRRKGKAEAEEMVE
SVAAAEIEEPEELEAGGGEEPEAAAGEETAEGEAPGEEARKKRRRKRRRGKKAKADETAAEEASAPEIAEEPAEEETTEA
PIPAEEAGEASEEVKKKRRRRKRRTPKRAGEATEAPATEVEPAEPRAAEPEPELAPAEASPESAEPAPKKTRAPRSRKKA
AEKPEKTEPAVALPEEQQAPAAAEATPVTPAEAKPAKKRATRKKTTSEPAPAPVQPPVPEPAADEPPKKKRAPRKKKEAP
TEGTPEAG
>Mature_807_residues
AKKMLINVMHPEESRVAIVEDGKLVDLDIEIEGSEQTRGNVYKGVVVRVEPGLQAAFVDIGLKRLGFLQMGEIHPSFWQW
RSDIPEESRSRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESESDRKKLKEKV
AELEIPEGYGYIVRTEALGKTKTELAKDLQYLVKLHQGIVDKAAAAKGPTLLHRELDVVIRTIRDYFTAEIDEVLVDSKD
VYKQAREFFKQTMPKFEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGVE
DTAYKTNLEAAEEAARQLRMRDLGGLIVLDFIDMRDKKHITAVEKTLKAALKGDKARVTVGRISQFGMLEMSRQRIRQTL
EQGSTLECPHCSGRGKVKSVENMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELAQIENEYDIVVTVKGKT
SFLMNQLELELVRREKPAHVETHEEHAEKVAEALAEGLPEAAEGTDVPAEASTAETEGKKKKRRRSRRKGKAEAEEMVES
VAAAEIEEPEELEAGGGEEPEAAAGEETAEGEAPGEEARKKRRRKRRRGKKAKADETAAEEASAPEIAEEPAEEETTEAP
IPAEEAGEASEEVKKKRRRRKRRTPKRAGEATEAPATEVEPAEPRAAEPEPELAPAEASPESAEPAPKKTRAPRSRKKAA
EKPEKTEPAVALPEEQQAPAAAEATPVTPAEAKPAKKRATRKKTTSEPAPAPVQPPVPEPAADEPPKKKRAPRKKKEAPT
EGTPEAG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=477, Percent_Identity=46.7505241090147, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI87082235, Length=439, Percent_Identity=41.002277904328, Blast_Score=311, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 89493; Mature: 89362

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKMLINVMHPEESRVAIVEDGKLVDLDIEIEGSEQTRGNVYKGVVVRVEPGLQAAFVD
CCCCCEEEEECCCCCCEEEEECCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHH
IGLKRLGFLQMGEIHPSFWQWRSDIPEESRSRRPRIQEVIRRGQELIVQVEKGERDMKGA
HHHHHHCCHHHCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHCCHHEEEEECCCCCCCCCH
ALTTYMSFPGRYMVLMPGSDSAGISRKVESESDRKKLKEKVAELEIPEGYGYIVRTEALG
HHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
KTKTELAKDLQYLVKLHQGIVDKAAAAKGPTLLHRELDVVIRTIRDYFTAEIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DVYKQAREFFKQTMPKFEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKTTGEKGVEDTAYKTNLEAAEEAARQLRMRDLGGLIVLDFIDMRDKKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHH
ITAVEKTLKAALKGDKARVTVGRISQFGMLEMSRQRIRQTLEQGSTLECPHCSGRGKVKS
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VENMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELAQIENEYDIVVTVKGK
HHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
TSFLMNQLELELVRREKPAHVETHEEHAEKVAEALAEGLPEAAEGTDVPAEASTAETEGK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
KKKRRRSRRKGKAEAEEMVESVAAAEIEEPEELEAGGGEEPEAAAGEETAEGEAPGEEAR
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KKRRRKRRRGKKAKADETAAEEASAPEIAEEPAEEETTEAPIPAEEAGEASEEVKKKRRR
HHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCHHCCCCCCCHHHCCCHHHHHHHHHHH
RKRRTPKRAGEATEAPATEVEPAEPRAAEPEPELAPAEASPESAEPAPKKTRAPRSRKKA
HHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHH
AEKPEKTEPAVALPEEQQAPAAAEATPVTPAEAKPAKKRATRKKTTSEPAPAPVQPPVPE
HCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PAADEPPKKKRAPRKKKEAPTEGTPEAG
CCCCCCCHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKKMLINVMHPEESRVAIVEDGKLVDLDIEIEGSEQTRGNVYKGVVVRVEPGLQAAFVD
CCCCEEEEECCCCCCEEEEECCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHH
IGLKRLGFLQMGEIHPSFWQWRSDIPEESRSRRPRIQEVIRRGQELIVQVEKGERDMKGA
HHHHHHCCHHHCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHCCHHEEEEECCCCCCCCCH
ALTTYMSFPGRYMVLMPGSDSAGISRKVESESDRKKLKEKVAELEIPEGYGYIVRTEALG
HHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
KTKTELAKDLQYLVKLHQGIVDKAAAAKGPTLLHRELDVVIRTIRDYFTAEIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DVYKQAREFFKQTMPKFEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKTTGEKGVEDTAYKTNLEAAEEAARQLRMRDLGGLIVLDFIDMRDKKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHH
ITAVEKTLKAALKGDKARVTVGRISQFGMLEMSRQRIRQTLEQGSTLECPHCSGRGKVKS
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VENMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELAQIENEYDIVVTVKGK
HHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
TSFLMNQLELELVRREKPAHVETHEEHAEKVAEALAEGLPEAAEGTDVPAEASTAETEGK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
KKKRRRSRRKGKAEAEEMVESVAAAEIEEPEELEAGGGEEPEAAAGEETAEGEAPGEEAR
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KKRRRKRRRGKKAKADETAAEEASAPEIAEEPAEEETTEAPIPAEEAGEASEEVKKKRRR
HHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCHHCCCCCCCHHHCCCHHHHHHHHHHH
RKRRTPKRAGEATEAPATEVEPAEPRAAEPEPELAPAEASPESAEPAPKKTRAPRSRKKA
HHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHH
AEKPEKTEPAVALPEEQQAPAAAEATPVTPAEAKPAKKRATRKKTTSEPAPAPVQPPVPE
HCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PAADEPPKKKRAPRKKKEAPTEGTPEAG
CCCCCCCHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]