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The map label for this gene is ubiE

Identifier: 39995973

GI number: 39995973

Start: 923469

End: 924239

Strand: Reverse

Name: ubiE

Synonym: GSU0867

Alternate gene names: 39995973

Gene position: 924239-923469 (Counterclockwise)

Preceding gene: 39995978

Following gene: 39995972

Centisome position: 24.23

GC content: 61.09

Gene sequence:

>771_bases
GTGAAGAATCTGAATTTATATCACTTCTTTGATTGCAAGGACAGAAAAATAACTATGTACCGGCTTACGGAAAAAGGCGA
ACGCATCCGCGACATGTTCAGCGACATCGCCCCCCGCTACGACTTTCTTAACCGGCTCCTTAGCTTCGGCGTGGACCGGC
GCTGGCGTCGAAACGCCGTCAAATGCATCCGGTGGAGCGAAGGCGGGCGCGTACTCGACGTGGCCACCGGCACCGGCGAC
GTGGCCCTGGAGATTGCCCGGCAAACGCCGCCGTCGGTCGCTATCGTAGGCGTCGACTTTTCCGAGGGAATGGTTGCCCT
GGGGCGCGACAAGGTGGCCGGCTCCCCCTACGCGGGCCGGATCACCATGGAAATCGCCCCCTGCGAGGCGATCCCTTTCC
CCGACGACACGTTCGATTCGGTGACCATCGCCTTCGGCATCCGCAACGTGGTTGATCGCAGCCAGGGGCTCTCCGAGATG
CTGCGGGTCCTTAAGCCGGGCGGCCGGGCTGTCATCCTCGAATTTTCCACCCCCCGTTCGCGGCTCTTCAAGAGGATCTA
CTCGTTCTATTTCCTCCGGGTTCTTCCCGTAATCGGCGGGCTGTTCTCGCAGTTCGGCGCCTACAAGTACCTGCCCGATT
CGGTGCTGGAGTTCCCGAGCCAGGAGGAGTTCAAGGCGCTCATGGCCTCGGTCGGCTTTCGCGACACCGCCCACCGGGAC
CAGACCTTCGGCATAGCCACCATCTACACCGGCGAAAAAGGGCTGAAATAG

Upstream 100 bases:

>100_bases
CCGGAAAAGGTGAAGCCGGCCCCGCCCGCATGCCGTCCCTCGAAAGTACTGGCATGGCCGGGCCGTTTTGTGCTACAAAC
GACAGACTTGTTCAGAATGC

Downstream 100 bases:

>100_bases
CGGAGTTTCTTGACATCACGCCCCGGATTCCGGTATAACAACGGCCGTTGTCACTTCTCTCCAAGGGGCACCCCATGTTC
GTGGTCGCCAATTTTCTGCT

Product: ubiquinone/menaquinone biosynthesis methyltransferase UbiE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAVKCIRWSEGGRVLDVATGTGD
VALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGRITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEM
LRVLKPGGRAVILEFSTPRSRLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRD
QTFGIATIYTGEKGLK

Sequences:

>Translated_256_residues
MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAVKCIRWSEGGRVLDVATGTGD
VALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGRITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEM
LRVLKPGGRAVILEFSTPRSRLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRD
QTFGIATIYTGEKGLK
>Mature_256_residues
MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAVKCIRWSEGGRVLDVATGTGD
VALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGRITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEM
LRVLKPGGRAVILEFSTPRSRLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRD
QTFGIATIYTGEKGLK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=264, Percent_Identity=34.0909090909091, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI48994983, Length=231, Percent_Identity=38.0952380952381, Blast_Score=180, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17557039, Length=237, Percent_Identity=35.0210970464135, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323526, Length=252, Percent_Identity=32.1428571428571, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24641702, Length=251, Percent_Identity=34.6613545816733, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_GEOSL (Q74EU2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951924.1
- ProteinModelPortal:   Q74EU2
- GeneID:   2687032
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0867
- NMPDR:   fig|243231.1.peg.862
- TIGR:   GSU0867
- HOGENOM:   HBG559580
- OMA:   GGAISKN
- ProtClustDB:   CLSK828068
- BioCyc:   GSUL243231:GSU_0867-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28925; Mature: 28925

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAV
CCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
KCIRWSEGGRVLDVATGTGDVALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGR
EEEEECCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCE
ITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEMLRVLKPGGRAVILEFSTPRS
EEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHH
RLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCC
QTFGIATIYTGEKGLK
CCCEEEEEEECCCCCC
>Mature Secondary Structure
MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAV
CCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
KCIRWSEGGRVLDVATGTGDVALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGR
EEEEECCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCE
ITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEMLRVLKPGGRAVILEFSTPRS
EEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHH
RLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCC
QTFGIATIYTGEKGLK
CCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA