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The map label for this gene is ntrX [H]

Identifier: 39995917

GI number: 39995917

Start: 869490

End: 870860

Strand: Reverse

Name: ntrX [H]

Synonym: GSU0811

Alternate gene names: 39995917

Gene position: 870860-869490 (Counterclockwise)

Preceding gene: 39995918

Following gene: 39995915

Centisome position: 22.83

GC content: 66.01

Gene sequence:

>1371_bases
GTGAACGAAACGATACTCGTCGTGGACGACGAACAGGACATCCGCACGGCCCTGGCCGGCATTCTGGAGGACGAGGGGTA
CCGGACCGCCTTCGCGCGCGACGGCGTCGAGGCCCTGGAGACGGTCCAGAAAGATCGGCCCGACCTGGTGCTTTTGGACA
TCTGGATGCCCAGGCTGGACGGCATGGAGACCCTCCAACGGCTCAAGACGGCCGACCCCGGCCTCACGGTGGTCATGATG
TCGGGCCACGGCACCATTGAAACGGCAGTGAAGTCCACCAAAATGGGGGCCTTCGACTTCATCGAGAAACCCCTCTCCCT
GGAAAAGATCCTGGTCACGGTCCGCAACGGCCTGGACGCAGGACGGCTCCGGGCCGAGAACGATTCGCTGCGCGCCCTGA
GCTTCAGGGGACACGAGATCATGGGGGAGTCGGAGGCCATGGGACGGCTGCGGGAGCAAGTCCGGCTGGCCGGCCCGGCC
GACGCGCCGGTGCTCATCACCGGCGAAAACGGGGCCGGCAAGGAACTGGCGGCCCGGTCGCTGCACTACCACAGCCCCCG
CCGCGACCGGCCCTTCGTGGAACTCGACTGCGCCGCCCTGCCCGAAGAACTCATCGAGAGCGAGCTCTTCGGCCACGAAA
AGGGCTTTGCCGGTGCCGCGACCCAGAAAAAGGGGAAGCTCGACCTGGCCAACGGCGGCACCATGTTTCTGGATGAGATC
GGCGACATGCCGCCCCGCGCCCAGGCGAAGCTCCTGCGCCTCATCCAGGAGCGGAAATTCGAGCGGGCCGGTGGAACTAG
GTCCATCGAGGCGGACGTGCGCATCATGGCCGCCAGCACGAGGAATCTGGAGGAAGAGGTCCGGGCGGGCCGCTTCAGCC
CCGACCTGTTTTACCGGCTGAACGTGGTCAGCCTCGCGGTGCCGCCGCTGCGGGAGCGGCAGGATGACATCGTGCCGCTG
GTGGAGCACTTCCTCGACATCTTCGCCCGCCGGGAGGGCCAGGAACGCAAAACCATGCCTCCCGAAGCCTTGGCCATCCT
GGCCCGGCACGACTGGCCCGGCAACGTGCGGGAGCTGCGTACCATCGTGGAGCGGCTCGTCATCATGACCCCGGGGCGGG
TGATCACCCCGGACAGCATTCCCCCGTCGATTGGCGAAGGCGCGGGTCGCGAGGGAGTTCTGCCCCGAAGCGAGGCCGCC
CTGGAGCCCTCGTCGCTCCGAGAGGCACGGGAAGAATTCGAGCGGGAATTCATCATCCAGAAACTGGAGGAGTACGGCTG
GAACATCTCGCGCACCGCCGACGCCATCGAGCTGGAGCGGAGCAACCTCTACCGCAAGATGAAGAGTTACGGGATCGACG
CTAAAAAATGA

Upstream 100 bases:

>100_bases
ATCATCACCGACCATCACGGGTTCATCCGGGTGCGGGACAACGAGCCCCGGGGGACCAAGTTCGTCATCGAACTGCCCGT
GACGGGATAGAGAGGATTAC

Downstream 100 bases:

>100_bases
CGGGCATATCCTTTCGCACGAAAAAGGCCGTCTTCCCATGGGGGAGACGGCCCTTTTTCTTCAGATCGCGGCCGCTACTT
CTTGATCACGCAGTCGATGA

Product: nitrogen regulation protein NtrX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNETILVVDDEQDIRTALAGILEDEGYRTAFARDGVEALETVQKDRPDLVLLDIWMPRLDGMETLQRLKTADPGLTVVMM
SGHGTIETAVKSTKMGAFDFIEKPLSLEKILVTVRNGLDAGRLRAENDSLRALSFRGHEIMGESEAMGRLREQVRLAGPA
DAPVLITGENGAGKELAARSLHYHSPRRDRPFVELDCAALPEELIESELFGHEKGFAGAATQKKGKLDLANGGTMFLDEI
GDMPPRAQAKLLRLIQERKFERAGGTRSIEADVRIMAASTRNLEEEVRAGRFSPDLFYRLNVVSLAVPPLRERQDDIVPL
VEHFLDIFARREGQERKTMPPEALAILARHDWPGNVRELRTIVERLVIMTPGRVITPDSIPPSIGEGAGREGVLPRSEAA
LEPSSLREAREEFEREFIIQKLEEYGWNISRTADAIELERSNLYRKMKSYGIDAKK

Sequences:

>Translated_456_residues
MNETILVVDDEQDIRTALAGILEDEGYRTAFARDGVEALETVQKDRPDLVLLDIWMPRLDGMETLQRLKTADPGLTVVMM
SGHGTIETAVKSTKMGAFDFIEKPLSLEKILVTVRNGLDAGRLRAENDSLRALSFRGHEIMGESEAMGRLREQVRLAGPA
DAPVLITGENGAGKELAARSLHYHSPRRDRPFVELDCAALPEELIESELFGHEKGFAGAATQKKGKLDLANGGTMFLDEI
GDMPPRAQAKLLRLIQERKFERAGGTRSIEADVRIMAASTRNLEEEVRAGRFSPDLFYRLNVVSLAVPPLRERQDDIVPL
VEHFLDIFARREGQERKTMPPEALAILARHDWPGNVRELRTIVERLVIMTPGRVITPDSIPPSIGEGAGREGVLPRSEAA
LEPSSLREAREEFEREFIIQKLEEYGWNISRTADAIELERSNLYRKMKSYGIDAKK
>Mature_456_residues
MNETILVVDDEQDIRTALAGILEDEGYRTAFARDGVEALETVQKDRPDLVLLDIWMPRLDGMETLQRLKTADPGLTVVMM
SGHGTIETAVKSTKMGAFDFIEKPLSLEKILVTVRNGLDAGRLRAENDSLRALSFRGHEIMGESEAMGRLREQVRLAGPA
DAPVLITGENGAGKELAARSLHYHSPRRDRPFVELDCAALPEELIESELFGHEKGFAGAATQKKGKLDLANGGTMFLDEI
GDMPPRAQAKLLRLIQERKFERAGGTRSIEADVRIMAASTRNLEEEVRAGRFSPDLFYRLNVVSLAVPPLRERQDDIVPL
VEHFLDIFARREGQERKTMPPEALAILARHDWPGNVRELRTIVERLVIMTPGRVITPDSIPPSIGEGAGREGVLPRSEAA
LEPSSLREAREEFEREFIIQKLEEYGWNISRTADAIELERSNLYRKMKSYGIDAKK

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=40.7894736842105, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=41.4096916299559, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=38.3297644539615, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=36.644591611479, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI87082117, Length=328, Percent_Identity=42.6829268292683, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=42.6791277258567, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=42.4528301886792, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789087, Length=320, Percent_Identity=43.125, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1787583, Length=324, Percent_Identity=37.3456790123457, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=347, Percent_Identity=35.7348703170029, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=36.2537764350453, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=32.4590163934426, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789828, Length=265, Percent_Identity=34.3396226415094, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=29.9145299145299, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=125, Percent_Identity=35.2, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=33.0097087378641, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=32.4786324786325, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=29.3103448275862, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50969; Mature: 50969

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETILVVDDEQDIRTALAGILEDEGYRTAFARDGVEALETVQKDRPDLVLLDIWMPRLD
CCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCC
GMETLQRLKTADPGLTVVMMSGHGTIETAVKSTKMGAFDFIEKPLSLEKILVTVRNGLDA
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCC
GRLRAENDSLRALSFRGHEIMGESEAMGRLREQVRLAGPADAPVLITGENGAGKELAARS
CCEECCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHH
LHYHSPRRDRPFVELDCAALPEELIESELFGHEKGFAGAATQKKGKLDLANGGTMFLDEI
HHCCCCCCCCCCEEECHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEHHHHH
GDMPPRAQAKLLRLIQERKFERAGGTRSIEADVRIMAASTRNLEEEVRAGRFSPDLFYRL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCHHHHHHCCCCCCCCEEEE
NVVSLAVPPLRERQDDIVPLVEHFLDIFARREGQERKTMPPEALAILARHDWPGNVRELR
EEHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
TIVERLVIMTPGRVITPDSIPPSIGEGAGREGVLPRSEAALEPSSLREAREEFEREFIIQ
HHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHH
KLEEYGWNISRTADAIELERSNLYRKMKSYGIDAKK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNETILVVDDEQDIRTALAGILEDEGYRTAFARDGVEALETVQKDRPDLVLLDIWMPRLD
CCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCC
GMETLQRLKTADPGLTVVMMSGHGTIETAVKSTKMGAFDFIEKPLSLEKILVTVRNGLDA
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCC
GRLRAENDSLRALSFRGHEIMGESEAMGRLREQVRLAGPADAPVLITGENGAGKELAARS
CCEECCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHH
LHYHSPRRDRPFVELDCAALPEELIESELFGHEKGFAGAATQKKGKLDLANGGTMFLDEI
HHCCCCCCCCCCEEECHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEHHHHH
GDMPPRAQAKLLRLIQERKFERAGGTRSIEADVRIMAASTRNLEEEVRAGRFSPDLFYRL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCHHHHHHCCCCCCCCEEEE
NVVSLAVPPLRERQDDIVPLVEHFLDIFARREGQERKTMPPEALAILARHDWPGNVRELR
EEHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
TIVERLVIMTPGRVITPDSIPPSIGEGAGREGVLPRSEAALEPSSLREAREEFEREFIIQ
HHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHH
KLEEYGWNISRTADAIELERSNLYRKMKSYGIDAKK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]