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The map label for this gene is oprF [H]

Identifier: 39995916

GI number: 39995916

Start: 868015

End: 869415

Strand: Direct

Name: oprF [H]

Synonym: GSU0810

Alternate gene names: 39995916

Gene position: 868015-869415 (Clockwise)

Preceding gene: 39995894

Following gene: 39995925

Centisome position: 22.76

GC content: 64.1

Gene sequence:

>1401_bases
ATGAAGGTGAGAACCATCGTTGGTGTCGCGTTTGCCGTCGCCCTGCTGGTCGCGTCGACGGCAGCCGCGGAGAACAGGGC
CGGTTTCTTTTCACTCAGTCCCTATTTCGGAGGCTATACCTTCGACGGGGAGCAGCACATCCGCACAGCGCCCACTTTCG
GCATCCGGGGCGGATACAACCTTACTCCAACCTGGGGGGTGGAGACCGTGTTCGGCTTCTCTCCGGCCGAGGAGACCAAG
GGCAGCCAGGTCGACGTGGACTCCTATACCTACCGGCTTGAGGGGCTCTACCATTTCATGCCGGAAAGCTCCCTTGTGCC
GTTCCTGGCGGTCGGCGCGGGCGGGACGACCCTCGACTACGCCGCCGGTTCCAACGGCCGAGATCGCACGGACGGCATCC
TCACCTACGGCGGCGGGGTCAAGTACTTCATCACCGACGTCGTGGCGGTACGGGGCGACGTGCGGGGCATCTTCGATCTG
GGATATTCGTACAGCAACGTCGAGTACACGGTCGGCTTGAGCTATCTGTTCGGCGGAGCCGCCCCGGCGGCCAGGGCAGT
TCCAGCGCCGCAGGCTGCGCCCGCACCGCCGGCTCCGGAGCCCGCCCCTGTCGTCGAACCCGAGCCTGCGCCGCTCCCCA
TGGCTGCCGAGCCGACGCCGGGCCTCATGAAGTACTGCATCACCCTCAACATTGAGTTCGACATCGACAAGGCCGACGTC
CGTCCCCAATACCACGACGAGGTGAAGCGGGTGGCTGACTTCCTGAAGGAGTATCCCTCGACCACGGCCGTGATTGAGGG
ACACACCGACAATGTGGGGGACGACGCTTACAACATGAGGCTTTCCCAGCGGCGCGCCGAAAGCGTGGTCAACTACCTGG
TGGACAATTTCGGGATCGACAGGACACGCCTCTCCGCCCGGGGCTTCGGCAAGACCCGCCCCATCGCCGACAACTCCACC
GAGGCGGGAAAGCAGCGCAACCGCCGGATCGAGGCGATCATCGACTGCGCCCTGGAGGACCCCAAGGTCATCGCGGCCCT
GCCTGACCGGCTCTGCATCGCCCTGAAGGTCCAGTTCGACACGGACAAGTGGGACATCAAGCCCCAATACCGCGGAGAGA
TCGCCAAGGTGGGCGATTTCATGACGCGGAATCCTACTACCACGGCAATCATCGAAGGGCATACCGACCGGGTCGGCACC
GCCGACCACAACATGAAGCTCTCCTTGCGCCGGGCCGAGGCGGTAGTCAATTACCTGGTCGAACACTTCGGCATCGAACG
TTCGCGCCTCTCCGCCAAGGGATACGGCTTCAGCCGGCCCATCGCCTACAACTCCACGGCCGAGGGCCGGGCCCTCAACC
GGCGGATCAACGCCATCATCGACTGCGTGATCAAGAAGTAG

Upstream 100 bases:

>100_bases
CGTGTAACTCTGCGACGTAGTTTGACAATTTGCCGTGCGCTGGTAAGGTTTGAAAGGGATTTGATCCCGATATCCGTTGC
CTGTCTACAGGGAGGTCGCC

Downstream 100 bases:

>100_bases
CGGCCGCGATCTGAAGAAAAAGGGCCGTCTCCCCCATGGGAAGACGGCCTTTTTCGTGCGAAAGGATATGCCCGTCATTT
TTTAGCGTCGATCCCGTAAC

Product: OmpA domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKVRTIVGVAFAVALLVASTAAAENRAGFFSLSPYFGGYTFDGEQHIRTAPTFGIRGGYNLTPTWGVETVFGFSPAEETK
GSQVDVDSYTYRLEGLYHFMPESSLVPFLAVGAGGTTLDYAAGSNGRDRTDGILTYGGGVKYFITDVVAVRGDVRGIFDL
GYSYSNVEYTVGLSYLFGGAAPAARAVPAPQAAPAPPAPEPAPVVEPEPAPLPMAAEPTPGLMKYCITLNIEFDIDKADV
RPQYHDEVKRVADFLKEYPSTTAVIEGHTDNVGDDAYNMRLSQRRAESVVNYLVDNFGIDRTRLSARGFGKTRPIADNST
EAGKQRNRRIEAIIDCALEDPKVIAALPDRLCIALKVQFDTDKWDIKPQYRGEIAKVGDFMTRNPTTTAIIEGHTDRVGT
ADHNMKLSLRRAEAVVNYLVEHFGIERSRLSAKGYGFSRPIAYNSTAEGRALNRRINAIIDCVIKK

Sequences:

>Translated_466_residues
MKVRTIVGVAFAVALLVASTAAAENRAGFFSLSPYFGGYTFDGEQHIRTAPTFGIRGGYNLTPTWGVETVFGFSPAEETK
GSQVDVDSYTYRLEGLYHFMPESSLVPFLAVGAGGTTLDYAAGSNGRDRTDGILTYGGGVKYFITDVVAVRGDVRGIFDL
GYSYSNVEYTVGLSYLFGGAAPAARAVPAPQAAPAPPAPEPAPVVEPEPAPLPMAAEPTPGLMKYCITLNIEFDIDKADV
RPQYHDEVKRVADFLKEYPSTTAVIEGHTDNVGDDAYNMRLSQRRAESVVNYLVDNFGIDRTRLSARGFGKTRPIADNST
EAGKQRNRRIEAIIDCALEDPKVIAALPDRLCIALKVQFDTDKWDIKPQYRGEIAKVGDFMTRNPTTTAIIEGHTDRVGT
ADHNMKLSLRRAEAVVNYLVEHFGIERSRLSAKGYGFSRPIAYNSTAEGRALNRRINAIIDCVIKK
>Mature_466_residues
MKVRTIVGVAFAVALLVASTAAAENRAGFFSLSPYFGGYTFDGEQHIRTAPTFGIRGGYNLTPTWGVETVFGFSPAEETK
GSQVDVDSYTYRLEGLYHFMPESSLVPFLAVGAGGTTLDYAAGSNGRDRTDGILTYGGGVKYFITDVVAVRGDVRGIFDL
GYSYSNVEYTVGLSYLFGGAAPAARAVPAPQAAPAPPAPEPAPVVEPEPAPLPMAAEPTPGLMKYCITLNIEFDIDKADV
RPQYHDEVKRVADFLKEYPSTTAVIEGHTDNVGDDAYNMRLSQRRAESVVNYLVDNFGIDRTRLSARGFGKTRPIADNST
EAGKQRNRRIEAIIDCALEDPKVIAALPDRLCIALKVQFDTDKWDIKPQYRGEIAKVGDFMTRNPTTTAIIEGHTDRVGT
ADHNMKLSLRRAEAVVNYLVEHFGIERSRLSAKGYGFSRPIAYNSTAEGRALNRRINAIIDCVIKK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=102, Percent_Identity=44.1176470588235, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787191, Length=304, Percent_Identity=26.6447368421053, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788957, Length=104, Percent_Identity=35.5769230769231, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=35.3535353535354, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 50819; Mature: 50819

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRTIVGVAFAVALLVASTAAAENRAGFFSLSPYFGGYTFDGEQHIRTAPTFGIRGGYN
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCEEECCCCCCCCCCC
LTPTWGVETVFGFSPAEETKGSQVDVDSYTYRLEGLYHFMPESSLVPFLAVGAGGTTLDY
CCCCCCCCEEECCCCHHHCCCCEEECCCEEEEECHHHEECCCCCCCCEEEECCCCCEEEE
AAGSNGRDRTDGILTYGGGVKYFITDVVAVRGDVRGIFDLGYSYSNVEYTVGLSYLFGGA
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEEHHHHHCCC
APAARAVPAPQAAPAPPAPEPAPVVEPEPAPLPMAAEPTPGLMKYCITLNIEFDIDKADV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCC
RPQYHDEVKRVADFLKEYPSTTAVIEGHTDNVGDDAYNMRLSQRRAESVVNYLVDNFGID
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RTRLSARGFGKTRPIADNSTEAGKQRNRRIEAIIDCALEDPKVIAALPDRLCIALKVQFD
HHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEHHHHHCCCCEEEEECCCCEEEEEEEEEC
TDKWDIKPQYRGEIAKVGDFMTRNPTTTAIIEGHTDRVGTADHNMKLSLRRAEAVVNYLV
CCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHH
EHFGIERSRLSAKGYGFSRPIAYNSTAEGRALNRRINAIIDCVIKK
HHHCCCHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVRTIVGVAFAVALLVASTAAAENRAGFFSLSPYFGGYTFDGEQHIRTAPTFGIRGGYN
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCEEECCCCCCCCCCC
LTPTWGVETVFGFSPAEETKGSQVDVDSYTYRLEGLYHFMPESSLVPFLAVGAGGTTLDY
CCCCCCCCEEECCCCHHHCCCCEEECCCEEEEECHHHEECCCCCCCCEEEECCCCCEEEE
AAGSNGRDRTDGILTYGGGVKYFITDVVAVRGDVRGIFDLGYSYSNVEYTVGLSYLFGGA
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEEHHHHHCCC
APAARAVPAPQAAPAPPAPEPAPVVEPEPAPLPMAAEPTPGLMKYCITLNIEFDIDKADV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCC
RPQYHDEVKRVADFLKEYPSTTAVIEGHTDNVGDDAYNMRLSQRRAESVVNYLVDNFGID
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RTRLSARGFGKTRPIADNSTEAGKQRNRRIEAIIDCALEDPKVIAALPDRLCIALKVQFD
HHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEHHHHHCCCCEEEEECCCCEEEEEEEEEC
TDKWDIKPQYRGEIAKVGDFMTRNPTTTAIIEGHTDRVGTADHNMKLSLRRAEAVVNYLV
CCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHH
EHFGIERSRLSAKGYGFSRPIAYNSTAEGRALNRRINAIIDCVIKK
HHHCCCHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]