Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 39995900

GI number: 39995900

Start: 852149

End: 853825

Strand: Reverse

Name: nirB [C]

Synonym: GSU0794

Alternate gene names: 39995900

Gene position: 853825-852149 (Counterclockwise)

Preceding gene: 39995901

Following gene: 39995899

Centisome position: 22.39

GC content: 62.61

Gene sequence:

>1677_bases
ATGAGCATTGTGGTGATCGGCGCAAACGCCGCCGGCGCCAAGGCGGCCTCGAAAGCGAAGCGGATCAACCCGACAGCCGA
AATAACCCTGATTGACCGGGGCACCTTCATTTCCTACGGTGCCTGCGGTATTCCCTATTTCGTTTCTGATACCGTTGAAG
ACGTGAAGGAGCTGATGAGCACGCCCGTCGGCGTCGTTCGCGACCATCAGTTCTTCAGGAAGGTCAAGGGCGTGACCGTC
AAGACCGCTACCGAGGTCATTGCGATCGATCGCGCAGCAAAAACCGTATGCATGCGTGATTGCCAGACATCCCGGGAAAC
GAAGCTCCCTTATGACCGCCTGGTTCTGGCCACCGGCAGCACCCCGTTCATCCCGCAGATCAGCAACGTCAATCTGGCCA
ATGTTCTCACCGTCAAGAGCATCGAGGATGCCGAACTGCTTAAGAGTCTGGCTGTTCCCGGCACCCGGGCCTGTATCGTC
GGCGGCGGACTGATCGGGCTGGAAACGGCTGAAGCGCTGCGTCACAAGGGGTTGCAGGTAGCGGTCGTCGAAATGCGGGA
CCAGATGCTGCCCGGCGTACTGGACTGGGAGATGGCGGCACTGGTGGAAAAGCAACTCAGGCAGCAGGGCGTCACCGTCA
TGACCGGCAGCGCGGTGACCGGCCTCGTCGGTGATGCCGCCGTCGAGGCGGTACAAATAGGGGACGTGCGGATCCCGGCC
GATCTGGTGGTCCTTGCTCCCGGCGTTGCCCCCAATGTCGAGCTGGCCCGCGGCGCCGGACTGGAGATAGGGCCCACCGG
AGCCATTGCGGTCGATACGCGCCAATGCACCACCGATCCCGACATCTACGCCTGCGGCGACTGCTGCGAAACCACGCATC
TCATTACCGGCAAAAAGGTGTTCATCCCCCTGGGGAGCACCGCCAACAAGCAGGGCCGGGTTGCCGGGATCAATGCCGCC
GGCGGCGAGGCAACCTTTGCCGGGGTCATCGGCACCAGCATTCTGCGGGTATTCGCTGTCAATGCCGGCAAAACGGGTCT
CACCGAGGCAGAGGCCAAGGCGCACGGGTTCGAGGTGGAGACTGTCCTGTCGCCGTCCCACGACAAGGCTCACTTCTTCC
CCGGGGCCAAGCCCATCATTCTCAAGCTGGTTGCCGAACGGGGCACCGGCCGGATTCTCGGCCTGCAAGCCGTGGGCGAA
GGCGCCGTCGACAAGCGGCTCGACGCCGCCGCCACTGCCATCACGTTCGGCGCAACCGCCGAGCAGGTGGCGCAGCTCGA
TCTCGCCTATGCGCCGCCCTACTCCGCCGCGATGGACAATCTGATCGTTGCCGCCGACATCCTGAAAAACAAGCTGGCCG
GCCATGCCCGCGGCATCACGCCCCGGGAGGTGAAACGCAAGTTCGATGAGGGTGATGACTTCATCCTGCTCGATGTCCGT
TCGCCGGCAGAGCACAACGCAGTGGGGATCGAAGGCGCCAAGCTGATCCCGCTCGGGATGCTGCGGGAGAAGCTTGAGGA
ACTGCCCAGGGACAAGGAGATCATCGCCTTTTGCAAGATCAGCCTTCGCGGCTATGAGGCCCAGAAGATCCTCGACGCGG
CAGGCTTCAGGAATGCAAAATTCATGGACGGCGGGATTCTGGCCTGGCCGTTCGCCCTCAGGACTTCTGACAAATAG

Upstream 100 bases:

>100_bases
GGCGGCAGGCCGGCCATCCCCTGGAAAAGTGATGGCAGCACCGGCAGCACCGGCGACGTGCCGGACACTACTATCTTGAA
CCGGAGATACGTGCATGAAG

Downstream 100 bases:

>100_bases
TGGGGCACCAGGGCGGCCCCTGCATGTTCATTTTCGGGACTCATCGCAACGAGCCCCGGCGGGGGGTGCCACAACCATCG
CAGGCGGCGTAATGAGTGCG

Product: pyridine nucleotide-disulfide oxidoreductase/rhodanese domain protein

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSIVVIGANAAGAKAASKAKRINPTAEITLIDRGTFISYGACGIPYFVSDTVEDVKELMSTPVGVVRDHQFFRKVKGVTV
KTATEVIAIDRAAKTVCMRDCQTSRETKLPYDRLVLATGSTPFIPQISNVNLANVLTVKSIEDAELLKSLAVPGTRACIV
GGGLIGLETAEALRHKGLQVAVVEMRDQMLPGVLDWEMAALVEKQLRQQGVTVMTGSAVTGLVGDAAVEAVQIGDVRIPA
DLVVLAPGVAPNVELARGAGLEIGPTGAIAVDTRQCTTDPDIYACGDCCETTHLITGKKVFIPLGSTANKQGRVAGINAA
GGEATFAGVIGTSILRVFAVNAGKTGLTEAEAKAHGFEVETVLSPSHDKAHFFPGAKPIILKLVAERGTGRILGLQAVGE
GAVDKRLDAAATAITFGATAEQVAQLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGITPREVKRKFDEGDDFILLDVR
SPAEHNAVGIEGAKLIPLGMLREKLEELPRDKEIIAFCKISLRGYEAQKILDAAGFRNAKFMDGGILAWPFALRTSDK

Sequences:

>Translated_558_residues
MSIVVIGANAAGAKAASKAKRINPTAEITLIDRGTFISYGACGIPYFVSDTVEDVKELMSTPVGVVRDHQFFRKVKGVTV
KTATEVIAIDRAAKTVCMRDCQTSRETKLPYDRLVLATGSTPFIPQISNVNLANVLTVKSIEDAELLKSLAVPGTRACIV
GGGLIGLETAEALRHKGLQVAVVEMRDQMLPGVLDWEMAALVEKQLRQQGVTVMTGSAVTGLVGDAAVEAVQIGDVRIPA
DLVVLAPGVAPNVELARGAGLEIGPTGAIAVDTRQCTTDPDIYACGDCCETTHLITGKKVFIPLGSTANKQGRVAGINAA
GGEATFAGVIGTSILRVFAVNAGKTGLTEAEAKAHGFEVETVLSPSHDKAHFFPGAKPIILKLVAERGTGRILGLQAVGE
GAVDKRLDAAATAITFGATAEQVAQLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGITPREVKRKFDEGDDFILLDVR
SPAEHNAVGIEGAKLIPLGMLREKLEELPRDKEIIAFCKISLRGYEAQKILDAAGFRNAKFMDGGILAWPFALRTSDK
>Mature_557_residues
SIVVIGANAAGAKAASKAKRINPTAEITLIDRGTFISYGACGIPYFVSDTVEDVKELMSTPVGVVRDHQFFRKVKGVTVK
TATEVIAIDRAAKTVCMRDCQTSRETKLPYDRLVLATGSTPFIPQISNVNLANVLTVKSIEDAELLKSLAVPGTRACIVG
GGLIGLETAEALRHKGLQVAVVEMRDQMLPGVLDWEMAALVEKQLRQQGVTVMTGSAVTGLVGDAAVEAVQIGDVRIPAD
LVVLAPGVAPNVELARGAGLEIGPTGAIAVDTRQCTTDPDIYACGDCCETTHLITGKKVFIPLGSTANKQGRVAGINAAG
GEATFAGVIGTSILRVFAVNAGKTGLTEAEAKAHGFEVETVLSPSHDKAHFFPGAKPIILKLVAERGTGRILGLQAVGEG
AVDKRLDAAATAITFGATAEQVAQLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGITPREVKRKFDEGDDFILLDVRS
PAEHNAVGIEGAKLIPLGMLREKLEELPRDKEIIAFCKISLRGYEAQKILDAAGFRNAKFMDGGILAWPFALRTSDK

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=334, Percent_Identity=28.4431137724551, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI65787454, Length=334, Percent_Identity=28.4431137724551, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI226437568, Length=334, Percent_Identity=28.4431137724551, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI91199540, Length=355, Percent_Identity=25.3521126760563, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1789765, Length=358, Percent_Identity=27.9329608938547, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=28.3737024221453, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082354, Length=318, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787352, Length=238, Percent_Identity=31.5126050420168, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=331, Percent_Identity=27.7945619335347, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788892, Length=288, Percent_Identity=24.6527777777778, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17559934, Length=288, Percent_Identity=24.3055555555556, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=25.6172839506173, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=25.6172839506173, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=25.6172839506173, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=25.6172839506173, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=25.6172839506173, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=25.6172839506173, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585130, Length=334, Percent_Identity=22.4550898203593, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 58837; Mature: 58706

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVVIGANAAGAKAASKAKRINPTAEITLIDRGTFISYGACGIPYFVSDTVEDVKELMS
CEEEEEECCCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHH
TPVGVVRDHQFFRKVKGVTVKTATEVIAIDRAAKTVCMRDCQTSRETKLPYDRLVLATGS
CCCCHHHHHHHHHHHCCCEEEEHHHEEEHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECC
TPFIPQISNVNLANVLTVKSIEDAELLKSLAVPGTRACIVGGGLIGLETAEALRHKGLQV
CCCCCCCCCCCHHHEEEECCCCHHHHHHHHCCCCCCEEEEECCEEEHHHHHHHHHCCCEE
AVVEMRDQMLPGVLDWEMAALVEKQLRQQGVTVMTGSAVTGLVGDAAVEAVQIGDVRIPA
EEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHEEEECCEECCC
DLVVLAPGVAPNVELARGAGLEIGPTGAIAVDTRQCTTDPDIYACGDCCETTHLITGKKV
CEEEECCCCCCCCEECCCCCCEECCCCEEEECCCCCCCCCCEEECCCHHCCCEEECCCEE
FIPLGSTANKQGRVAGINAAGGEATFAGVIGTSILRVFAVNAGKTGLTEAEAKAHGFEVE
EEECCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHEECCCCCCCCHHHHHHCCEEEE
TVLSPSHDKAHFFPGAKPIILKLVAERGTGRILGLQAVGEGAVDKRLDAAATAITFGATA
EEECCCCCCCEECCCCCCEEHHEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECCCH
EQVAQLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGITPREVKRKFDEGDDFILLDVR
HHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SPAEHNAVGIEGAKLIPLGMLREKLEELPRDKEIIAFCKISLRGYEAQKILDAAGFRNAK
CCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCCE
FMDGGILAWPFALRTSDK
EECCCEEEEEEEEECCCC
>Mature Secondary Structure 
SIVVIGANAAGAKAASKAKRINPTAEITLIDRGTFISYGACGIPYFVSDTVEDVKELMS
EEEEEECCCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHH
TPVGVVRDHQFFRKVKGVTVKTATEVIAIDRAAKTVCMRDCQTSRETKLPYDRLVLATGS
CCCCHHHHHHHHHHHCCCEEEEHHHEEEHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECC
TPFIPQISNVNLANVLTVKSIEDAELLKSLAVPGTRACIVGGGLIGLETAEALRHKGLQV
CCCCCCCCCCCHHHEEEECCCCHHHHHHHHCCCCCCEEEEECCEEEHHHHHHHHHCCCEE
AVVEMRDQMLPGVLDWEMAALVEKQLRQQGVTVMTGSAVTGLVGDAAVEAVQIGDVRIPA
EEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHEEEECCEECCC
DLVVLAPGVAPNVELARGAGLEIGPTGAIAVDTRQCTTDPDIYACGDCCETTHLITGKKV
CEEEECCCCCCCCEECCCCCCEECCCCEEEECCCCCCCCCCEEECCCHHCCCEEECCCEE
FIPLGSTANKQGRVAGINAAGGEATFAGVIGTSILRVFAVNAGKTGLTEAEAKAHGFEVE
EEECCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHEECCCCCCCCHHHHHHCCEEEE
TVLSPSHDKAHFFPGAKPIILKLVAERGTGRILGLQAVGEGAVDKRLDAAATAITFGATA
EEECCCCCCCEECCCCCCEEHHEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECCCH
EQVAQLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGITPREVKRKFDEGDDFILLDVR
HHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SPAEHNAVGIEGAKLIPLGMLREKLEELPRDKEIIAFCKISLRGYEAQKILDAAGFRNAK
CCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCCE
FMDGGILAWPFALRTSDK
EECCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA