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The map label for this gene is hybO [H]

Identifier: 39995888

GI number: 39995888

Start: 842270

End: 843373

Strand: Direct

Name: hybO [H]

Synonym: GSU0782

Alternate gene names: 39995888

Gene position: 842270-843373 (Clockwise)

Preceding gene: 39995887

Following gene: 39995889

Centisome position: 22.08

GC content: 64.13

Gene sequence:

>1104_bases
ATGGGAAAGGAAGCTGTGCTTCGCGGCGGCGTGTCGCGAAGGGATTTCATGAAGACCTGTGTTGCAGCCACCGCTATCAT
GGGGCTGCCCTACAGCATGCACACAAAGGTGGCCGAAGCTGCCCAGAAGGCCGGCCGGCCGCCGGTGATCTGGCTTCACT
TCCAGGAGTGCACCGGCTGTTCAGAGTCGCTTCTCCGTTCGGGGCACCCGGATGTTTCCGAACTGATACTTGACATGATT
TCGCTGGACTACCATGAAACCCTCATGGTCGGCTCGGGGCATCAGGCGGAGCAGTCGCTCCACGACTCCATGAAGGCGAA
CCACGGCAAGTACATCCTGGTCGTGGAGGGTGGCATCCCCACCAAGGACAACGGCATCTACTGCAAGGTGGGCGGCAAGA
CCGCCGTGGATTCCCTGACCACCGCCGCCGAAGGGGCTGCCGCCATCATCACCATCGGCACCTGCGCCTCATACGGCGGC
ATCCAGTCGGCTCCTCCCAACCCTACGGGCGCCGTGGGGGTCCGCGACCTGATCAAGAACAAGCCGATCGTCAACATTCC
CGGCTGTCCGCCCAACCCCTACAACTTCCTCTCCACGGTCCTCTACTACCTGACGTTCAACAAGCTCCCCGAGCTCGACG
CACTGGGCCGTCCCAAGTTTGCCTACGGCCGGAAGATTCACGAGCACTGCGAGCGCCGTCCCCACTTCGACGCGGGCCGC
TTCGCCACCGCCTACGGCGACAAGACCCATGCCGAGGGGTACTGCCTCTACAAGCTGGGCTGCAAGGGGCCGGCGACATT
CGCCAACTGCTCCGTCACCCGTTTCAACGACGTGGGGGTATGGCCCGTTTCCGTCGGCCACCCGTGCATTGGTTGCACCG
AGCCGGATATCCTCTTCAAGAAGCCCATCGCCGAGAAGCTCCAGATCCACCTGCCCACGCCGCCCGACACCTATGCTCCG
GCCGAACTGGGGCAGAAGGGTCCCGGCGTGAGCCCGGCGGCCACCGGTCTCGTTGGCCTGGCCGGCGGCGTAGCCCTGGG
TGCCGGCGCCATGCTGGCCAAGAAGCTGCCCAGCGGGGAGGAAGGTCATGAAAAGCACGACTAG

Upstream 100 bases:

>100_bases
TTTTATGTAGAAAAAAGATGTGCTATTTTTAATCTTGTACTGCAGGGCTAAAGTGATTTCGGGCTACTGACAATCACGCA
TCATACCGACAGGGGGATTT

Downstream 100 bases:

>100_bases
ACGCGATTTCCTCAAGATGATGGGGATGACCGGCGCCGCCTGCCTGGCGTGCGCCGCGCCCCTGTCGGCCTCCACGGGCC
CGGCCGGCGAGAGCGAAACC

Product: nickel-dependent hydrogenase small subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MGKEAVLRGGVSRRDFMKTCVAATAIMGLPYSMHTKVAEAAQKAGRPPVIWLHFQECTGCSESLLRSGHPDVSELILDMI
SLDYHETLMVGSGHQAEQSLHDSMKANHGKYILVVEGGIPTKDNGIYCKVGGKTAVDSLTTAAEGAAAIITIGTCASYGG
IQSAPPNPTGAVGVRDLIKNKPIVNIPGCPPNPYNFLSTVLYYLTFNKLPELDALGRPKFAYGRKIHEHCERRPHFDAGR
FATAYGDKTHAEGYCLYKLGCKGPATFANCSVTRFNDVGVWPVSVGHPCIGCTEPDILFKKPIAEKLQIHLPTPPDTYAP
AELGQKGPGVSPAATGLVGLAGGVALGAGAMLAKKLPSGEEGHEKHD

Sequences:

>Translated_367_residues
MGKEAVLRGGVSRRDFMKTCVAATAIMGLPYSMHTKVAEAAQKAGRPPVIWLHFQECTGCSESLLRSGHPDVSELILDMI
SLDYHETLMVGSGHQAEQSLHDSMKANHGKYILVVEGGIPTKDNGIYCKVGGKTAVDSLTTAAEGAAAIITIGTCASYGG
IQSAPPNPTGAVGVRDLIKNKPIVNIPGCPPNPYNFLSTVLYYLTFNKLPELDALGRPKFAYGRKIHEHCERRPHFDAGR
FATAYGDKTHAEGYCLYKLGCKGPATFANCSVTRFNDVGVWPVSVGHPCIGCTEPDILFKKPIAEKLQIHLPTPPDTYAP
AELGQKGPGVSPAATGLVGLAGGVALGAGAMLAKKLPSGEEGHEKHD
>Mature_366_residues
GKEAVLRGGVSRRDFMKTCVAATAIMGLPYSMHTKVAEAAQKAGRPPVIWLHFQECTGCSESLLRSGHPDVSELILDMIS
LDYHETLMVGSGHQAEQSLHDSMKANHGKYILVVEGGIPTKDNGIYCKVGGKTAVDSLTTAAEGAAAIITIGTCASYGGI
QSAPPNPTGAVGVRDLIKNKPIVNIPGCPPNPYNFLSTVLYYLTFNKLPELDALGRPKFAYGRKIHEHCERRPHFDAGRF
ATAYGDKTHAEGYCLYKLGCKGPATFANCSVTRFNDVGVWPVSVGHPCIGCTEPDILFKKPIAEKLQIHLPTPPDTYAPA
ELGQKGPGVSPAATGLVGLAGGVALGAGAMLAKKLPSGEEGHEKHD

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=326, Percent_Identity=54.2944785276074, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1787206, Length=291, Percent_Identity=47.0790378006873, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 38915; Mature: 38784

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEAVLRGGVSRRDFMKTCVAATAIMGLPYSMHTKVAEAAQKAGRPPVIWLHFQECTGC
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCH
SESLLRSGHPDVSELILDMISLDYHETLMVGSGHQAEQSLHDSMKANHGKYILVVEGGIP
HHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TKDNGIYCKVGGKTAVDSLTTAAEGAAAIITIGTCASYGGIQSAPPNPTGAVGVRDLIKN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECHHCCCCCCCCCCCCCCCHHHHHHHCC
KPIVNIPGCPPNPYNFLSTVLYYLTFNKLPELDALGRPKFAYGRKIHEHCERRPHFDAGR
CCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
FATAYGDKTHAEGYCLYKLGCKGPATFANCSVTRFNDVGVWPVSVGHPCIGCTEPDILFK
EEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHH
KPIAEKLQIHLPTPPDTYAPAELGQKGPGVSPAATGLVGLAGGVALGAGAMLAKKLPSGE
CCCCCCEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGHEKHD
CCCCCCC
>Mature Secondary Structure 
GKEAVLRGGVSRRDFMKTCVAATAIMGLPYSMHTKVAEAAQKAGRPPVIWLHFQECTGC
CCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCH
SESLLRSGHPDVSELILDMISLDYHETLMVGSGHQAEQSLHDSMKANHGKYILVVEGGIP
HHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TKDNGIYCKVGGKTAVDSLTTAAEGAAAIITIGTCASYGGIQSAPPNPTGAVGVRDLIKN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECHHCCCCCCCCCCCCCCCHHHHHHHCC
KPIVNIPGCPPNPYNFLSTVLYYLTFNKLPELDALGRPKFAYGRKIHEHCERRPHFDAGR
CCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
FATAYGDKTHAEGYCLYKLGCKGPATFANCSVTRFNDVGVWPVSVGHPCIGCTEPDILFK
EEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHH
KPIAEKLQIHLPTPPDTYAPAELGQKGPGVSPAATGLVGLAGGVALGAGAMLAKKLPSGE
CCCCCCEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGHEKHD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]