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The map label for this gene is fdnH [C]

Identifier: 39995884

GI number: 39995884

Start: 837976

End: 838809

Strand: Direct

Name: fdnH [C]

Synonym: GSU0778

Alternate gene names: 39995884

Gene position: 837976-838809 (Clockwise)

Preceding gene: 39995883

Following gene: 39995885

Centisome position: 21.97

GC content: 62.59

Gene sequence:

>834_bases
ATGAGTGCCGGAGCCATCGACTATTCAAAAACCAAGACGTTCCTGATCGATACCACCAAGTGCACCGGCTGCCGCGGCTG
CCAGGTGGCCTGCAAGCAGTGGAACCAGCTGAAGGCCGAGAATACGACCTTCTTCACCGGCGAAGGCTACCAGAACCCGC
CGGGGATGAGTGAATTCACCTTCACCCGGGTCAAGTTCAAGGACTACCAGAAGCACGGCCAGAACGAGTTCGCCTTCTAC
AAGGAGATGTGCATGCACTGCAACGATCCCGCATGCGCCTCCGTCTGCCCCGTGGGTGCCTTCGAGAAGACCGCCGAAGG
GCCGGTGGTCTACCACTCCAAGAAGTGCATCGGCTGCCGTTTCTGCATGGTGGCATGCCCCTTCGGCATCCCCAAGTACG
AGTGGAGCAAGGCGTTCCCGCTGGTCAAGAAGTGCACGGGCTGCTACAGCCGGGTGAAGAACGGCCTCGATCCCGCCTGC
GCCACCGCCTGCCCCACGGCCATAACCTACGGTCCCCGGGATGAAATGATCAAGGAGGCCGAGAAGCGGATGTCCGCCAG
GCCCGACCGCTACGTGAAAGTGGTCTACGGCAAGGAAGAGGCCGGCGGCACCACGGTCCTCTACCTGACCCAGCAGCCCC
TGGACGAGCTCGGCTTCAAGCCGGTCACCAAGCGGCCGCTGCCGTCCTACACCTGGCAGGCCCTGCGCCTCGTGCCGGGG
ATCTTCCTGACCGTGGGCGGCACCCTCTCCCTCATTACCTGGTTCCAGCACCGCAAGGACCGGGTCCGGAAGGAAGAAGA
GGAGCAGAAGAACCGCAAGGAGGGGAACCAATGA

Upstream 100 bases:

>100_bases
ACAGCGGCAACGTCCTGACCCCCACCGTGGGGTGCGCCAACACCGGCATTCCGGAATTCAAGGCGTTCCTCTGCAACATT
GAAAAAGGAGGTAAGGCCGC

Downstream 100 bases:

>100_bases
CCGCAGCACGTATCGTCATCAATGAAATCAAGGGATACCACCGGTTCATCAAGTTTCTGATGGTCCTGGTGGGTGCGGCG
GCCGTGGCCTCCGCGGTCCG

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSAGAIDYSKTKTFLIDTTKCTGCRGCQVACKQWNQLKAENTTFFTGEGYQNPPGMSEFTFTRVKFKDYQKHGQNEFAFY
KEMCMHCNDPACASVCPVGAFEKTAEGPVVYHSKKCIGCRFCMVACPFGIPKYEWSKAFPLVKKCTGCYSRVKNGLDPAC
ATACPTAITYGPRDEMIKEAEKRMSARPDRYVKVVYGKEEAGGTTVLYLTQQPLDELGFKPVTKRPLPSYTWQALRLVPG
IFLTVGGTLSLITWFQHRKDRVRKEEEEQKNRKEGNQ

Sequences:

>Translated_277_residues
MSAGAIDYSKTKTFLIDTTKCTGCRGCQVACKQWNQLKAENTTFFTGEGYQNPPGMSEFTFTRVKFKDYQKHGQNEFAFY
KEMCMHCNDPACASVCPVGAFEKTAEGPVVYHSKKCIGCRFCMVACPFGIPKYEWSKAFPLVKKCTGCYSRVKNGLDPAC
ATACPTAITYGPRDEMIKEAEKRMSARPDRYVKVVYGKEEAGGTTVLYLTQQPLDELGFKPVTKRPLPSYTWQALRLVPG
IFLTVGGTLSLITWFQHRKDRVRKEEEEQKNRKEGNQ
>Mature_276_residues
SAGAIDYSKTKTFLIDTTKCTGCRGCQVACKQWNQLKAENTTFFTGEGYQNPPGMSEFTFTRVKFKDYQKHGQNEFAFYK
EMCMHCNDPACASVCPVGAFEKTAEGPVVYHSKKCIGCRFCMVACPFGIPKYEWSKAFPLVKKCTGCYSRVKNGLDPACA
TACPTAITYGPRDEMIKEAEKRMSARPDRYVKVVYGKEEAGGTTVLYLTQQPLDELGFKPVTKRPLPSYTWQALRLVPGI
FLTVGGTLSLITWFQHRKDRVRKEEEEQKNRKEGNQ

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=210, Percent_Identity=39.5238095238095, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790326, Length=281, Percent_Identity=32.3843416370107, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789370, Length=245, Percent_Identity=33.469387755102, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787122, Length=182, Percent_Identity=38.4615384615385, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787872, Length=170, Percent_Identity=40, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI2367345, Length=191, Percent_Identity=30.3664921465969, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI226510944, Length=164, Percent_Identity=28.6585365853659, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI2367245, Length=153, Percent_Identity=32.6797385620915, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788811, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=32.3232323232323, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789079, Length=128, Percent_Identity=28.90625, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082180, Length=215, Percent_Identity=25.1162790697674, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 31260; Mature: 31129

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGAIDYSKTKTFLIDTTKCTGCRGCQVACKQWNQLKAENTTFFTGEGYQNPPGMSEFT
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
FTRVKFKDYQKHGQNEFAFYKEMCMHCNDPACASVCPVGAFEKTAEGPVVYHSKKCIGCR
EEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHCCCCCEEEECCCHHHHH
FCMVACPFGIPKYEWSKAFPLVKKCTGCYSRVKNGLDPACATACPTAITYGPRDEMIKEA
HHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEECCCHHHHHHHH
EKRMSARPDRYVKVVYGKEEAGGTTVLYLTQQPLDELGFKPVTKRPLPSYTWQALRLVPG
HHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHH
IFLTVGGTLSLITWFQHRKDRVRKEEEEQKNRKEGNQ
HEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAGAIDYSKTKTFLIDTTKCTGCRGCQVACKQWNQLKAENTTFFTGEGYQNPPGMSEFT
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
FTRVKFKDYQKHGQNEFAFYKEMCMHCNDPACASVCPVGAFEKTAEGPVVYHSKKCIGCR
EEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHCCCCCEEEECCCHHHHH
FCMVACPFGIPKYEWSKAFPLVKKCTGCYSRVKNGLDPACATACPTAITYGPRDEMIKEA
HHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEECCCHHHHHHHH
EKRMSARPDRYVKVVYGKEEAGGTTVLYLTQQPLDELGFKPVTKRPLPSYTWQALRLVPG
HHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHH
IFLTVGGTLSLITWFQHRKDRVRKEEEEQKNRKEGNQ
HEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]