Click here to switch to the map view.

The map label for this gene is cooH [H]

Identifier: 39995849

GI number: 39995849

Start: 789025

End: 790542

Strand: Direct

Name: cooH [H]

Synonym: GSU0743

Alternate gene names: 39995849

Gene position: 789025-790542 (Clockwise)

Preceding gene: 39995848

Following gene: 39995850

Centisome position: 20.69

GC content: 69.76

Gene sequence:

>1518_bases
ATGAGCGCCCGGTTCCTCACCCTGCACAACGGTTCAGCCGTCCCCTTCCACGAGGTGCCGCGCCTGGAGTTGACCGCCTT
CCTCCAGCAGATGATCGACGCCGTGGACCGGGGTTGGCGCGTCTCCTCCTACTTCGGCATCCCCACGGAGGCCGGGGCCA
CCCTCTGCTGTGCCCTCTCCTCCACGTCCGACGGCAGCATCGCCCTGGCAACGGCAGACGCGGCCGGGTCGTTCCCGTCC
ATCGCCCGGTTCTGCCCCCAGGTGCAGCTCTTCGAGCGGGAGATCGCCGAGCAGTGCGGCCTTCCCGCCGAGGATCACCC
CTGGTTCAAGCCGGTCCGGTTCCACCGTGCCTTCGTTCCCGGCCGGGATGCCTGGGGACGCGCCGCCGGTGAGCCGATCC
CCGTGGGGGTCATGGACTACTACCGGGTGGAAGGGGATGAGGTCCACGAAGTGGCGGTGGGCCCGGTCCACGCCGGGATC
ATCGAGCCGGGCCACTTCCGGTTCCAGTGCCACGGGGAAGAGGTCTTTCACCTGGAGATCTCCCTGGGCTACCAGTACCG
GGGGATCGAGCAGGGGCTCATGGGCGGCCCCGACCCTCGCACCCCCTTCCGCATGGAGACCATTGCCGGTGACACCACCA
TCGGCCACATGCAGGCATACTGCATGGCCCTGGAAGCCCTGGCGGGCACCCGGGTCCCGGCTCGGGCCGAGGCCCTGCGC
GGCATTGCCCTGGAGCTGGAGCGGCTGGCGAACCACACCGGCGACCTGGGGGCCATTGCCGGCGACGTGGGCTACCTCCC
CACCGCCTCCTTCTGCGGCCGGATCAGGGGCGATTTCCTCAACATGAGTGCGGTCCTCTGCGGCAGCCGCTTCGGCCGGG
GGCTGGTGCGTCCCGGCGGGGTCCTCTTCGACCTGGGGCCGGCCGAACGCGACGACCTCCTGGCCCGCCTCGACGCCGCC
CAGTCGGACCTGCAGGGTGCCGTGGAAATCCTCTGGGACGCGCCGTCGGTCATGAGCAGGCTGGAGAACACCGGCACCGT
GCCGGCCGACACCGCCCGGGAACTGGGCCTGGTGGGACCTGCGGCAAGGGCCTGCGGCCTCAACCGCGACGTGCGGCGCG
ACCACCCCTACGGCATCTATCGGATGGCCCAGATCCCGGTGGTCACCGCCCACCACGGCGACGTCTGCGCCCGGTCCACG
GTGCGCTGGCTGGAGGCGGGGAAATCCCTCGACTTCATCCGGGAGCAGCTCAAACAACTCCCAGGAGGCGGCTGCCGGAA
CGAGGCGGGCTCCCTGCAGCCGGACCGGATTGCCGTGGCCCTCACCGAAGGGTGGCGGGGCGAGGTCTGTCACGTGGCGA
TCACCGGCGCTGACGGTCGCTTCCTGCGCTACAAGGTGACCGACCCCTCGTTCCACAACTGGCCGGGGCTCGCCATGGCG
CTGCGGGGGGAGCAGATCTCCGACTTCCCCCTCTGCAACAAGAGTTTCAATCTGTCGTACTGCGGGTTTGATCTGTAG

Upstream 100 bases:

>100_bases
CCCTGGCCCTGCTGGGGCTGATCCTGATGCTGGGGGTCTGGCTGCCTGAACCGCTCCTGCGGCTCCTGGGCGAAGGGGCC
GCTCTGCTGGAGGCCAAACC

Downstream 100 bases:

>100_bases
GGGGAGCGGTGGCCTGCGGAAGGCGGACGGCTGGAAGCGGGAAGGTCCGGGGCGGTGCTCCCGCTCCGAAAATATCCAAG
CGTCACCTGTCGTATTTCAC

Product: NAD-dependent dehydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSARFLTLHNGSAVPFHEVPRLELTAFLQQMIDAVDRGWRVSSYFGIPTEAGATLCCALSSTSDGSIALATADAAGSFPS
IARFCPQVQLFEREIAEQCGLPAEDHPWFKPVRFHRAFVPGRDAWGRAAGEPIPVGVMDYYRVEGDEVHEVAVGPVHAGI
IEPGHFRFQCHGEEVFHLEISLGYQYRGIEQGLMGGPDPRTPFRMETIAGDTTIGHMQAYCMALEALAGTRVPARAEALR
GIALELERLANHTGDLGAIAGDVGYLPTASFCGRIRGDFLNMSAVLCGSRFGRGLVRPGGVLFDLGPAERDDLLARLDAA
QSDLQGAVEILWDAPSVMSRLENTGTVPADTARELGLVGPAARACGLNRDVRRDHPYGIYRMAQIPVVTAHHGDVCARST
VRWLEAGKSLDFIREQLKQLPGGGCRNEAGSLQPDRIAVALTEGWRGEVCHVAITGADGRFLRYKVTDPSFHNWPGLAMA
LRGEQISDFPLCNKSFNLSYCGFDL

Sequences:

>Translated_505_residues
MSARFLTLHNGSAVPFHEVPRLELTAFLQQMIDAVDRGWRVSSYFGIPTEAGATLCCALSSTSDGSIALATADAAGSFPS
IARFCPQVQLFEREIAEQCGLPAEDHPWFKPVRFHRAFVPGRDAWGRAAGEPIPVGVMDYYRVEGDEVHEVAVGPVHAGI
IEPGHFRFQCHGEEVFHLEISLGYQYRGIEQGLMGGPDPRTPFRMETIAGDTTIGHMQAYCMALEALAGTRVPARAEALR
GIALELERLANHTGDLGAIAGDVGYLPTASFCGRIRGDFLNMSAVLCGSRFGRGLVRPGGVLFDLGPAERDDLLARLDAA
QSDLQGAVEILWDAPSVMSRLENTGTVPADTARELGLVGPAARACGLNRDVRRDHPYGIYRMAQIPVVTAHHGDVCARST
VRWLEAGKSLDFIREQLKQLPGGGCRNEAGSLQPDRIAVALTEGWRGEVCHVAITGADGRFLRYKVTDPSFHNWPGLAMA
LRGEQISDFPLCNKSFNLSYCGFDL
>Mature_504_residues
SARFLTLHNGSAVPFHEVPRLELTAFLQQMIDAVDRGWRVSSYFGIPTEAGATLCCALSSTSDGSIALATADAAGSFPSI
ARFCPQVQLFEREIAEQCGLPAEDHPWFKPVRFHRAFVPGRDAWGRAAGEPIPVGVMDYYRVEGDEVHEVAVGPVHAGII
EPGHFRFQCHGEEVFHLEISLGYQYRGIEQGLMGGPDPRTPFRMETIAGDTTIGHMQAYCMALEALAGTRVPARAEALRG
IALELERLANHTGDLGAIAGDVGYLPTASFCGRIRGDFLNMSAVLCGSRFGRGLVRPGGVLFDLGPAERDDLLARLDAAQ
SDLQGAVEILWDAPSVMSRLENTGTVPADTARELGLVGPAARACGLNRDVRRDHPYGIYRMAQIPVVTAHHGDVCARSTV
RWLEAGKSLDFIREQLKQLPGGGCRNEAGSLQPDRIAVALTEGWRGEVCHVAITGADGRFLRYKVTDPSFHNWPGLAMAL
RGEQISDFPLCNKSFNLSYCGFDL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=362, Percent_Identity=23.7569060773481, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4758786, Length=362, Percent_Identity=23.7569060773481, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789076, Length=443, Percent_Identity=30.4740406320542, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788832, Length=479, Percent_Identity=29.2275574112735, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693162, Length=516, Percent_Identity=22.4806201550388, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17555284, Length=228, Percent_Identity=29.8245614035088, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568379, Length=221, Percent_Identity=28.0542986425339, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221459469, Length=301, Percent_Identity=24.5847176079734, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24638644, Length=281, Percent_Identity=25.6227758007117, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 54801; Mature: 54670

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARFLTLHNGSAVPFHEVPRLELTAFLQQMIDAVDRGWRVSSYFGIPTEAGATLCCALS
CCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEC
STSDGSIALATADAAGSFPSIARFCPQVQLFEREIAEQCGLPAEDHPWFKPVRFHRAFVP
CCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEECC
GRDAWGRAAGEPIPVGVMDYYRVEGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLEI
CCCCCCCCCCCCCCCCHHHHEECCCCCEEEEEECCEEECCCCCCCEEEEECCCEEEEEEE
SLGYQYRGIEQGLMGGPDPRTPFRMETIAGDTTIGHMQAYCMALEALAGTRVPARAEALR
EECCEECCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GIALELERLANHTGDLGAIAGDVGYLPTASFCGRIRGDFLNMSAVLCGSRFGRGLVRPGG
HHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
VLFDLGPAERDDLLARLDAAQSDLQGAVEILWDAPSVMSRLENTGTVPADTARELGLVGP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCCCC
AARACGLNRDVRRDHPYGIYRMAQIPVVTAHHGDVCARSTVRWLEAGKSLDFIREQLKQL
HHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHC
PGGGCRNEAGSLQPDRIAVALTEGWRGEVCHVAITGADGRFLRYKVTDPSFHNWPGLAMA
CCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEE
LRGEQISDFPLCNKSFNLSYCGFDL
ECCCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
SARFLTLHNGSAVPFHEVPRLELTAFLQQMIDAVDRGWRVSSYFGIPTEAGATLCCALS
CCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEC
STSDGSIALATADAAGSFPSIARFCPQVQLFEREIAEQCGLPAEDHPWFKPVRFHRAFVP
CCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEECC
GRDAWGRAAGEPIPVGVMDYYRVEGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLEI
CCCCCCCCCCCCCCCCHHHHEECCCCCEEEEEECCEEECCCCCCCEEEEECCCEEEEEEE
SLGYQYRGIEQGLMGGPDPRTPFRMETIAGDTTIGHMQAYCMALEALAGTRVPARAEALR
EECCEECCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GIALELERLANHTGDLGAIAGDVGYLPTASFCGRIRGDFLNMSAVLCGSRFGRGLVRPGG
HHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
VLFDLGPAERDDLLARLDAAQSDLQGAVEILWDAPSVMSRLENTGTVPADTARELGLVGP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCCCC
AARACGLNRDVRRDHPYGIYRMAQIPVVTAHHGDVCARSTVRWLEAGKSLDFIREQLKQL
HHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHC
PGGGCRNEAGSLQPDRIAVALTEGWRGEVCHVAITGADGRFLRYKVTDPSFHNWPGLAMA
CCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEE
LRGEQISDFPLCNKSFNLSYCGFDL
ECCCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]