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The map label for this gene is hyfF [H]

Identifier: 39995848

GI number: 39995848

Start: 787592

End: 789028

Strand: Direct

Name: hyfF [H]

Synonym: GSU0742

Alternate gene names: 39995848

Gene position: 787592-789028 (Clockwise)

Preceding gene: 39995847

Following gene: 39995849

Centisome position: 20.65

GC content: 67.78

Gene sequence:

>1437_bases
ATGCTGACCGCCGCCGTTCTCCTGCCGCTCGTAGGCGCCCTCGTGGCCTGGATCGTCCCGGACAACCGGCTCCGCCCCCT
GGTGCTGCCGGCTGTGGCCGTCCCGCATTTCCTCCTGACCCTGGCGCTCCTGGCGTGGACCCCGCCGCCGTCCCCCCAGG
GGTGGATCTTCCTGGACCCCCTCGGCAAGATCGTGCTCCTCTGCATCAGTACCCTGTTCCTGATCTGCGCCGTCTACGCG
GTGGGCTACCTGGCCTACCGCCGGGACCGCTCCAACCGGGTCCTCTGCATGGGGCTGCTGGCGTGCCTCTCGGCCATGAC
CCTGGTGACCGCCTCCCAGCACCTGGGGCTCCTCTGGGTGGCGGTGGAGACCACCACCGTGACCATGGCGCCCCTGATCT
ACTTCAACCGCAACGCCCGCTCCATCGAGGCCACCTGGAAGTACCTCCTGATTTGCTCCGTGGGGATCGCCCTGGCGCTT
CTGGGGCTTTTCTTCCTGGCCTACGCGACGCTTGTCGCCCACCGGGACGCCACCCTGCTCCTGGGGCCGATCCTTGCCTC
GGCCCGGGAGCTGAACCCGGGCTGGCTCCACGCCTCCTTCATCTTCATGCTGGTGGGATTCGGCTCCAAGATGGGGATCG
CGCCGCTCCATTCCTGGAAGCCCGATGCCTACGGCGAGGCGCCGGGGCTCGTGGGGGCGCTCCTGGCCGGGGGACTGGTC
AACTGCGCGTTTCTGGCCATCCTGCGGGTCTACCAGATCAGCCTCGCCTCCGGCGGGGAATACCTTTTCTTCCAGAAGGC
ACTCCTGACCATGGGGCTCATCTCCATGGGGCTGGCAGCGGTCTTTATGGCGCGCCAGGCCGATTTCAAGCGGATGCTGG
CCTATTCCAGTGTGGAGCACGTGGGCATCCTTGCGGTGGCCCTGGGGCTCGGCAAGGGGGCCCTGTTCGGGGCGCTCTTC
CACCTGCTGAACAACGGCCTCACCAAGGGGGTCCTCTTTCTCTCATCGGGCAACATCCACCGCAGTTTCAACAGCAAGAG
CACCGAGGTGGTGCGCGGCGCCCTGGCCCGGCTCCCCTGGTCCGCCGGCCTCTTCCTGGCCGGTTTCATCGCCATAACCG
GGTCGCCGCCGTTCTCCCCCTTCGTGAGCGAGTTCTCCATCGTGAGCAGCGCCTTCGTGGAGGGGCGCTACCTGGTGGGG
GGCCTCTTCCTTTTCTTCCTGGCCGTGATCTTCATCGGCATGGCCCTCACGGTGCTGCCCCTGGTCATGGGCGTGCCCCC
GGCCGACGCGGAACGGACCGACTACCGCGACCGGCTTCTGACCGTGGGGCCTCCCCTGGCCCTGCTGGGGCTGATCCTGA
TGCTGGGGGTCTGGCTGCCTGAACCGCTCCTGCGGCTCCTGGGCGAAGGGGCCGCTCTGCTGGAGGCCAAACCATGA

Upstream 100 bases:

>100_bases
GTGGGGATCTTCGTCATGGGAATCGTCATTAACCACATCAGCCGCGAGTTCTCCTCCGTCGACACCTCGCGCCTGAGCGC
CCTGAAGGAGGAGTAGGCCC

Downstream 100 bases:

>100_bases
GCGCCCGGTTCCTCACCCTGCACAACGGTTCAGCCGTCCCCTTCCACGAGGTGCCGCGCCTGGAGTTGACCGCCTTCCTC
CAGCAGATGATCGACGCCGT

Product: NAD-dependent dehydrogenase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLTAAVLLPLVGALVAWIVPDNRLRPLVLPAVAVPHFLLTLALLAWTPPPSPQGWIFLDPLGKIVLLCISTLFLICAVYA
VGYLAYRRDRSNRVLCMGLLACLSAMTLVTASQHLGLLWVAVETTTVTMAPLIYFNRNARSIEATWKYLLICSVGIALAL
LGLFFLAYATLVAHRDATLLLGPILASARELNPGWLHASFIFMLVGFGSKMGIAPLHSWKPDAYGEAPGLVGALLAGGLV
NCAFLAILRVYQISLASGGEYLFFQKALLTMGLISMGLAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALFGALF
HLLNNGLTKGVLFLSSGNIHRSFNSKSTEVVRGALARLPWSAGLFLAGFIAITGSPPFSPFVSEFSIVSSAFVEGRYLVG
GLFLFFLAVIFIGMALTVLPLVMGVPPADAERTDYRDRLLTVGPPLALLGLILMLGVWLPEPLLRLLGEGAALLEAKP

Sequences:

>Translated_478_residues
MLTAAVLLPLVGALVAWIVPDNRLRPLVLPAVAVPHFLLTLALLAWTPPPSPQGWIFLDPLGKIVLLCISTLFLICAVYA
VGYLAYRRDRSNRVLCMGLLACLSAMTLVTASQHLGLLWVAVETTTVTMAPLIYFNRNARSIEATWKYLLICSVGIALAL
LGLFFLAYATLVAHRDATLLLGPILASARELNPGWLHASFIFMLVGFGSKMGIAPLHSWKPDAYGEAPGLVGALLAGGLV
NCAFLAILRVYQISLASGGEYLFFQKALLTMGLISMGLAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALFGALF
HLLNNGLTKGVLFLSSGNIHRSFNSKSTEVVRGALARLPWSAGLFLAGFIAITGSPPFSPFVSEFSIVSSAFVEGRYLVG
GLFLFFLAVIFIGMALTVLPLVMGVPPADAERTDYRDRLLTVGPPLALLGLILMLGVWLPEPLLRLLGEGAALLEAKP
>Mature_478_residues
MLTAAVLLPLVGALVAWIVPDNRLRPLVLPAVAVPHFLLTLALLAWTPPPSPQGWIFLDPLGKIVLLCISTLFLICAVYA
VGYLAYRRDRSNRVLCMGLLACLSAMTLVTASQHLGLLWVAVETTTVTMAPLIYFNRNARSIEATWKYLLICSVGIALAL
LGLFFLAYATLVAHRDATLLLGPILASARELNPGWLHASFIFMLVGFGSKMGIAPLHSWKPDAYGEAPGLVGALLAGGLV
NCAFLAILRVYQISLASGGEYLFFQKALLTMGLISMGLAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALFGALF
HLLNNGLTKGVLFLSSGNIHRSFNSKSTEVVRGALARLPWSAGLFLAGFIAITGSPPFSPFVSEFSIVSSAFVEGRYLVG
GLFLFFLAVIFIGMALTVLPLVMGVPPADAERTDYRDRLLTVGPPLALLGLILMLGVWLPEPLLRLLGEGAALLEAKP

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=178, Percent_Identity=28.6516853932584, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788831, Length=429, Percent_Identity=34.965034965035, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788827, Length=403, Percent_Identity=30.0248138957816, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788613, Length=240, Percent_Identity=30, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788829, Length=349, Percent_Identity=27.2206303724928, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=236, Percent_Identity=29.6610169491525, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI2367154, Length=352, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51143; Mature: 51143

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAAVLLPLVGALVAWIVPDNRLRPLVLPAVAVPHFLLTLALLAWTPPPSPQGWIFLDP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECH
LGKIVLLCISTLFLICAVYAVGYLAYRRDRSNRVLCMGLLACLSAMTLVTASQHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVETTTVTMAPLIYFNRNARSIEATWKYLLICSVGIALALLGLFFLAYATLVAHRDATLL
EEEHHHEEEHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LGPILASARELNPGWLHASFIFMLVGFGSKMGIAPLHSWKPDAYGEAPGLVGALLAGGLV
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NCAFLAILRVYQISLASGGEYLFFQKALLTMGLISMGLAAVFMARQADFKRMLAYSSVEH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGILAVALGLGKGALFGALFHLLNNGLTKGVLFLSSGNIHRSFNSKSTEVVRGALARLPW
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHCEEEEECCCEEECCCCCHHHHHHHHHHHCCC
SAGLFLAGFIAITGSPPFSPFVSEFSIVSSAFVEGRYLVGGLFLFFLAVIFIGMALTVLP
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVMGVPPADAERTDYRDRLLTVGPPLALLGLILMLGVWLPEPLLRLLGEGAALLEAKP
HHHCCCCCCCCCCCHHHCEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure
MLTAAVLLPLVGALVAWIVPDNRLRPLVLPAVAVPHFLLTLALLAWTPPPSPQGWIFLDP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECH
LGKIVLLCISTLFLICAVYAVGYLAYRRDRSNRVLCMGLLACLSAMTLVTASQHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVETTTVTMAPLIYFNRNARSIEATWKYLLICSVGIALALLGLFFLAYATLVAHRDATLL
EEEHHHEEEHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LGPILASARELNPGWLHASFIFMLVGFGSKMGIAPLHSWKPDAYGEAPGLVGALLAGGLV
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NCAFLAILRVYQISLASGGEYLFFQKALLTMGLISMGLAAVFMARQADFKRMLAYSSVEH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGILAVALGLGKGALFGALFHLLNNGLTKGVLFLSSGNIHRSFNSKSTEVVRGALARLPW
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHCEEEEECCCEEECCCCCHHHHHHHHHHHCCC
SAGLFLAGFIAITGSPPFSPFVSEFSIVSSAFVEGRYLVGGLFLFFLAVIFIGMALTVLP
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVMGVPPADAERTDYRDRLLTVGPPLALLGLILMLGVWLPEPLLRLLGEGAALLEAKP
HHHCCCCCCCCCCCHHHCEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]