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The map label for this gene is dfrA [H]

Identifier: 39995793

GI number: 39995793

Start: 724370

End: 725356

Strand: Reverse

Name: dfrA [H]

Synonym: GSU0687

Alternate gene names: 39995793

Gene position: 725356-724370 (Counterclockwise)

Preceding gene: 39995794

Following gene: 39995792

Centisome position: 19.02

GC content: 65.65

Gene sequence:

>987_bases
ATGAAGGTTTTTGTCACCGGCGCGACCGGTTTTATAGGGGCGAGCATCGTCCGGGAATTGCTGAAGGACGGCTGCCACGT
GCGGGTGCTTGCCCGCCCGGGCTCCGACCGTCGGAACCTCGCGGGGCTGGACGTGGAGATCTGCGAAGGGGATCTCCGGG
ATCGGCAGGCGCTGGAGCACGGCCTTGCCGGCTGCGAGGTTCTTTACCACGCCGCCGCCGACTACCGACTCTGGACCCGC
ACGCCGGCGGCAATGTATGCGGCCAACGTGGACGGCACCCGCAACATCCTGGAGGCGGCCCTGCGGCGCGGCATCGCCAG
GGTGGTCTACACCAGCAGCGTAGGCACCCTCGGCAATCCCGGCAACGGCACCCCCGGAACCGAGACGACGCCGGTCACCT
TCGCCGACATGGTCGGTCACTACAAAAAAAGCAAGTTCCTGGCCGAGCGGGAAGCGGAGGCGTTCATCGCCCGGGGTCTC
CCGCTGGTGATCGTAAATCCCTCCACGCCGGTGGGCCCCCACGACGTGAAGCCGACTCCCACGGGCAAGATCATCGTCGA
CTTCCTTAACCGGAAGATGCCCGCCTACCTGGACACCGGCCTCAATATCATCGATGTGGAGGACTGTGCCCGGGGACACC
TGCTGGCGGCACGGCACGGCCGGATCGGTGAAAAGTACATCCTGGGGCACGAGAACCTGACCCTGCGGGAGATCTTCGCC
CTGTTGGCCAGGCTCACGGGGATTCCGGCGCCCAGGGTGCGGCTCCCCCATACCCCGATTCTCATGGCCGCTTACGTGAA
CGAGGCACTGGCCAAGCTTACGGGAAAAGAGCCGCTCATCCCGTTGGCAGGGGTGCAGATGGCCAAAAAATTCATGTTCT
TCGAATCGTCCAAGGCGACGGGGGAGCTGGGGCTCCAACGCCGTCCCGCCGTGGATGCCCTGCGCAGGGCCGTGGAGTGG
TTCCGCGCCAACGGGTATGCCCGGTGA

Upstream 100 bases:

>100_bases
CGGACGCTTGCGGGCAAAGGGCTCTGACAGCCTCGACAAGTAAGTGGATTACGCGTAGGGGCGGTTCACCAACCGCCCCT
ACATCAGTTTTGAAGGCACT

Downstream 100 bases:

>100_bases
ACTGAGGCCGAAGGCTAAGGATTCCCTTTGCCCTCGGCCGGGAGGTTACGCATGTCCACGCTGCTCGACACAATCACCTG
CCCCGCCGATCTGAAAAAGA

Product: dihydroflavonol 4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKVFVTGATGFIGASIVRELLKDGCHVRVLARPGSDRRNLAGLDVEICEGDLRDRQALEHGLAGCEVLYHAAADYRLWTR
TPAAMYAANVDGTRNILEAALRRGIARVVYTSSVGTLGNPGNGTPGTETTPVTFADMVGHYKKSKFLAEREAEAFIARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRKMPAYLDTGLNIIDVEDCARGHLLAARHGRIGEKYILGHENLTLREIFA
LLARLTGIPAPRVRLPHTPILMAAYVNEALAKLTGKEPLIPLAGVQMAKKFMFFESSKATGELGLQRRPAVDALRRAVEW
FRANGYAR

Sequences:

>Translated_328_residues
MKVFVTGATGFIGASIVRELLKDGCHVRVLARPGSDRRNLAGLDVEICEGDLRDRQALEHGLAGCEVLYHAAADYRLWTR
TPAAMYAANVDGTRNILEAALRRGIARVVYTSSVGTLGNPGNGTPGTETTPVTFADMVGHYKKSKFLAEREAEAFIARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRKMPAYLDTGLNIIDVEDCARGHLLAARHGRIGEKYILGHENLTLREIFA
LLARLTGIPAPRVRLPHTPILMAAYVNEALAKLTGKEPLIPLAGVQMAKKFMFFESSKATGELGLQRRPAVDALRRAVEW
FRANGYAR
>Mature_328_residues
MKVFVTGATGFIGASIVRELLKDGCHVRVLARPGSDRRNLAGLDVEICEGDLRDRQALEHGLAGCEVLYHAAADYRLWTR
TPAAMYAANVDGTRNILEAALRRGIARVVYTSSVGTLGNPGNGTPGTETTPVTFADMVGHYKKSKFLAEREAEAFIARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRKMPAYLDTGLNIIDVEDCARGHLLAARHGRIGEKYILGHENLTLREIFA
LLARLTGIPAPRVRLPHTPILMAAYVNEALAKLTGKEPLIPLAGVQMAKKFMFFESSKATGELGLQRRPAVDALRRAVEW
FRANGYAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=356, Percent_Identity=25.8426966292135, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI310132178, Length=333, Percent_Identity=25.8258258258258, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI310113012, Length=333, Percent_Identity=25.8258258258258, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI193211614, Length=342, Percent_Identity=26.6081871345029, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8393516, Length=342, Percent_Identity=26.6081871345029, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI239745448, Length=333, Percent_Identity=25.5255255255255, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI87081792, Length=347, Percent_Identity=25.9365994236311, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71987463, Length=339, Percent_Identity=30.6784660766962, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 35725; Mature: 35725

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFVTGATGFIGASIVRELLKDGCHVRVLARPGSDRRNLAGLDVEICEGDLRDRQALEH
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHH
GLAGCEVLYHAAADYRLWTRTPAAMYAANVDGTRNILEAALRRGIARVVYTSSVGTLGNP
HHHHHHHHHHHHCCCEEEECCCHHHEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
GNGTPGTETTPVTFADMVGHYKKSKFLAEREAEAFIARGLPLVIVNPSTPVGPHDVKPTP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNRKMPAYLDTGLNIIDVEDCARGHLLAARHGRIGEKYILGHENLTLREIFA
CCHHHHHHHCCCCCHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
LLARLTGIPAPRVRLPHTPILMAAYVNEALAKLTGKEPLIPLAGVQMAKKFMFFESSKAT
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
GELGLQRRPAVDALRRAVEWFRANGYAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVFVTGATGFIGASIVRELLKDGCHVRVLARPGSDRRNLAGLDVEICEGDLRDRQALEH
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHH
GLAGCEVLYHAAADYRLWTRTPAAMYAANVDGTRNILEAALRRGIARVVYTSSVGTLGNP
HHHHHHHHHHHHCCCEEEECCCHHHEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
GNGTPGTETTPVTFADMVGHYKKSKFLAEREAEAFIARGLPLVIVNPSTPVGPHDVKPTP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNRKMPAYLDTGLNIIDVEDCARGHLLAARHGRIGEKYILGHENLTLREIFA
CCHHHHHHHCCCCCHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
LLARLTGIPAPRVRLPHTPILMAAYVNEALAKLTGKEPLIPLAGVQMAKKFMFFESSKAT
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
GELGLQRRPAVDALRRAVEWFRANGYAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]