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The map label for this gene is ilvE [H]

Identifier: 39995762

GI number: 39995762

Start: 691898

End: 692971

Strand: Direct

Name: ilvE [H]

Synonym: GSU0656

Alternate gene names: 39995762

Gene position: 691898-692971 (Clockwise)

Preceding gene: 39995759

Following gene: 39995763

Centisome position: 18.14

GC content: 57.73

Gene sequence:

>1074_bases
ATGGAAATCAGAGTTGCACCACTTCCCGCTGAGCAGAAGAAGCACAAGGCCACTGACGAAACAGCTCTAGGCTTTGGTCG
TCTTTTTACCGACCGGATGCTTGTTGCCGAATGGAAAGTCGGGCAGGGCTGGGTCGATGCGCGAATTGAGCCCTATGCTC
CGTTTTCGCTGGATCCTGCCTGTGCGGTATTTCACTATGCCCAGGAGATTTTTGAAGGACTCAAGGCGTATAAGTGGGCG
GATGGTTCCATAGCACTCTTTCGCCCTGAAATGAATGCCCGCCGCTTCAATCATTCAGCAGAGCGTCTCTGCATGCCCGA
GGTGCCGGAAGATCTTTTCATCGGCGGCATCGAGCAACTGGTGGCGCTCGAGCGCGACTGGATACCGGCCTCCGCGGGGA
CTTCCCTGTATATCCGCCCCGCAATGATTGCCGTGGAGCCGGCTCTCGGCGTGAAACCTGCCGATCATTACTACTTTTTT
GTGATTCTTTCACCGGTGGGCGCCTATTACGCCTCTGGATTCAATCCGGTGAAAATCATGGTGGAAGACCAGTATGTCCG
CGCCGTGCCCGGCGGCACCGGTGAAGCCAAGACAGGCGGCAATTATGCTTCGTCGCTCAGGGCCGGCCTTGAGGCAAAAA
AGAAGGGGTTTGACCAGGTCCTCTGGCTTGACGGGGTCCACCGTCGCTACATCGAGGAGGTTGGGGCTATGAATATGCTG
TTTGCCTACGGCAAGACCATAGTCACCTCGCCTCTCACGGGCACCATTCTGAACGGCGTTACCCGCGACTCGGTTCTGAA
ACTGGCCGCGAGCCGCGGTTACACCATTGAAGAGCGGTTGATTGACGTGAACGATCTCATGGCCGATATTCGTGCCGGCA
AGGTGACCGAGGCTTTCGGCAGTGGCACTGCCGCTGTCGTCAGTCCCGTTGGAATCCTGGCGTACAAGGGCGAGAGCGTG
ACCGTAGGCGACGGTGGCGTAGGGCCGATCACCCGGGAACTCTATGATACCCTTACCGGCATCCAGACGGGCCGGATTCC
CGACACCTTCGGCTGGACCCGCAAAATTGCCTGA

Upstream 100 bases:

>100_bases
GCTGGCAGCGCAAAACATCTTGCCGCGTCTGGGATGCTGTGATAAACCGATTTATATAAACTGAGCCGACGCTTGGCGCT
GGCTAACGGAGGTCCTGAAG

Downstream 100 bases:

>100_bases
TTGTGCCAAGGAAGGCGGCGGCAACGCCGCCTTCCCTTTTTTCGTCACATTCTGTTGCCATGGCAAATATGGAGATTGCT
CCGGACACCCTAGCGAAGGT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEIRVAPLPAEQKKHKATDETALGFGRLFTDRMLVAEWKVGQGWVDARIEPYAPFSLDPACAVFHYAQEIFEGLKAYKWA
DGSIALFRPEMNARRFNHSAERLCMPEVPEDLFIGGIEQLVALERDWIPASAGTSLYIRPAMIAVEPALGVKPADHYYFF
VILSPVGAYYASGFNPVKIMVEDQYVRAVPGGTGEAKTGGNYASSLRAGLEAKKKGFDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTSPLTGTILNGVTRDSVLKLAASRGYTIEERLIDVNDLMADIRAGKVTEAFGSGTAAVVSPVGILAYKGESV
TVGDGGVGPITRELYDTLTGIQTGRIPDTFGWTRKIA

Sequences:

>Translated_357_residues
MEIRVAPLPAEQKKHKATDETALGFGRLFTDRMLVAEWKVGQGWVDARIEPYAPFSLDPACAVFHYAQEIFEGLKAYKWA
DGSIALFRPEMNARRFNHSAERLCMPEVPEDLFIGGIEQLVALERDWIPASAGTSLYIRPAMIAVEPALGVKPADHYYFF
VILSPVGAYYASGFNPVKIMVEDQYVRAVPGGTGEAKTGGNYASSLRAGLEAKKKGFDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTSPLTGTILNGVTRDSVLKLAASRGYTIEERLIDVNDLMADIRAGKVTEAFGSGTAAVVSPVGILAYKGESV
TVGDGGVGPITRELYDTLTGIQTGRIPDTFGWTRKIA
>Mature_357_residues
MEIRVAPLPAEQKKHKATDETALGFGRLFTDRMLVAEWKVGQGWVDARIEPYAPFSLDPACAVFHYAQEIFEGLKAYKWA
DGSIALFRPEMNARRFNHSAERLCMPEVPEDLFIGGIEQLVALERDWIPASAGTSLYIRPAMIAVEPALGVKPADHYYFF
VILSPVGAYYASGFNPVKIMVEDQYVRAVPGGTGEAKTGGNYASSLRAGLEAKKKGFDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTSPLTGTILNGVTRDSVLKLAASRGYTIEERLIDVNDLMADIRAGKVTEAFGSGTAAVVSPVGILAYKGESV
TVGDGGVGPITRELYDTLTGIQTGRIPDTFGWTRKIA

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=44.0579710144928, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=44.0579710144928, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=44.0579710144928, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI50658084, Length=348, Percent_Identity=39.6551724137931, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=39.4202898550725, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=39.4202898550725, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI258614015, Length=286, Percent_Identity=39.5104895104895, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI48994963, Length=319, Percent_Identity=30.7210031347962, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=359, Percent_Identity=38.9972144846797, Blast_Score=257, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI17565728, Length=353, Percent_Identity=35.1274787535411, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322608, Length=376, Percent_Identity=40.9574468085106, Blast_Score=282, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6322002, Length=371, Percent_Identity=39.622641509434, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24641779, Length=354, Percent_Identity=38.9830508474576, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38950; Mature: 38950

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRVAPLPAEQKKHKATDETALGFGRLFTDRMLVAEWKVGQGWVDARIEPYAPFSLDPA
CCEEECCCCCHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCEECCCCCCCCCCCCCHH
CAVFHYAQEIFEGLKAYKWADGSIALFRPEMNARRFNHSAERLCMPEVPEDLFIGGIEQL
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHCCCHHHCCCCCCCHHHHHHHHHHH
VALERDWIPASAGTSLYIRPAMIAVEPALGVKPADHYYFFVILSPVGAYYASGFNPVKIM
HHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDQYVRAVPGGTGEAKTGGNYASSLRAGLEAKKKGFDQVLWLDGVHRRYIEEVGAMNML
EECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
FAYGKTIVTSPLTGTILNGVTRDSVLKLAASRGYTIEERLIDVNDLMADIRAGKVTEAFG
HHHCCHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHC
SGTAAVVSPVGILAYKGESVTVGDGGVGPITRELYDTLTGIQTGRIPDTFGWTRKIA
CCCHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEIRVAPLPAEQKKHKATDETALGFGRLFTDRMLVAEWKVGQGWVDARIEPYAPFSLDPA
CCEEECCCCCHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCEECCCCCCCCCCCCCHH
CAVFHYAQEIFEGLKAYKWADGSIALFRPEMNARRFNHSAERLCMPEVPEDLFIGGIEQL
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHCCCHHHCCCCCCCHHHHHHHHHHH
VALERDWIPASAGTSLYIRPAMIAVEPALGVKPADHYYFFVILSPVGAYYASGFNPVKIM
HHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDQYVRAVPGGTGEAKTGGNYASSLRAGLEAKKKGFDQVLWLDGVHRRYIEEVGAMNML
EECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
FAYGKTIVTSPLTGTILNGVTRDSVLKLAASRGYTIEERLIDVNDLMADIRAGKVTEAFG
HHHCCHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHC
SGTAAVVSPVGILAYKGESVTVGDGGVGPITRELYDTLTGIQTGRIPDTFGWTRKIA
CCCHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]