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The map label for this gene is aspA [H]

Identifier: 39995587

GI number: 39995587

Start: 510727

End: 512118

Strand: Direct

Name: aspA [H]

Synonym: GSU0479

Alternate gene names: 39995587

Gene position: 510727-512118 (Clockwise)

Preceding gene: 39995586

Following gene: 39995588

Centisome position: 13.39

GC content: 66.59

Gene sequence:

>1392_bases
GTGACGTTCCGGGTCGAGAAGGATACCCTCGGTGAGGTGCGGGTTCCGGCGGACGCCTACTACGGCGCCCAGACGGTCCG
GGCCGTGGAGAATTTCCCCATCTCCGGGCTGCGGCCCCATCCGGCCCTCGTCCGGGCGACGGTGCTCGTCAAAATGTGTG
CGGCCCGGGCCAACATGGCCACGGGCAGGCTCGATTCCCGGCTTGGAGACGCTATCGTCCGGGCGGCCGACGAGTGCCTG
GCCGGTGCCTTTGATGCCCACTTCGTGGTGGACCCGTTTCAAGCCGGCGCCGGCACCTCCCACAACATGAACGTGAACGA
GGTCCTCGCCAACCGGGCCAATGAACTGCTGGGAGAGGAGCGGGGCAGGTATGCTCCCGTCCATCCCAACGACCACGTGA
ACATGGCCCAGTCCACCAACGACGTCTTCCCCACGGCCATGCGTCTGGCGGCCCTTCGGGTGGCGGGGGATCTCCGGCCG
GCCCTAGAGGGACTCGTTGCCGCGCTTCGGAAAAAGTCCGCTGAATTCGACGGCATTCTCAAGAGCGGCCGGACTCACCT
GCAGGATGCGGTGCCGATCCGTCTCGGCCAGGAGTTCGAGGCGTGGGCCGTGGCGCTGGAGAAGAACCTGGCCGCCATCG
AGGGGGCGGTGCCGGGCCTTGCGGAACTGGGTATCGGCGGCACCGCGGCAGGTACCGGCATGAACGCCGAGCCGGCCTAC
ATCGATCTTGTGGTGGCAGAGCTCGCCCGGGAAACCGGCTTTCCCCTCGTACGGGGTGCGAACCTGGTTGAGCGGATGCA
GAACATGGACCCCTTCGTGGCCCTCTCCTCCGCTCTCAAGGGACTTGCCGTAAACCTCGCCCGTATCGCCAACGACCTGC
GGCTCCTTTCCTCTGGCCCCCGGACCGGGCTGGCCGAGATCGCGCTGCCGGCGCTGCAGCCCGGCTCCTCTATCATGCCG
GGGAAGGTCAACCCGGTCATGGCCGAGGTGACCGACATGGTCGCCTTTCAAGTGATGGGTGCGGACACGACAATCATGCT
CGCCGCCCAGGCAGGGCAGCTGGAGCTTAACGTCATGATGCCGGTCATCGCGTTCAACCTTCTGTTCAGCCTTGAAATCC
TGAAGAACACGGTGCCCAAGCTGGCTGATGCCTGCATCGCCGGCATCACTGCGGACCGGGAGCGCTGCCTCCGGTACCTG
GAGCAGTCGGTAGGACTGGCCACGGTGCTCGCGCCCTACATCGGCTATGCCGCGGCCGCGGAGGTGGCCAAGGAATCGAC
TGCCACGGGCCGGAGTATCCGGCAGATCGTGGAAGACCGGCAGCTCCTGTCGGCCGGGCGTCTGGCGGAGATCCTCGACC
CCTTCCCTCTCACCAGCCCTGGCGTTCCGTGA

Upstream 100 bases:

>100_bases
GCCTTGCGCGACGAGGAGGCGGAGCACTTGCGGATCGTAACCGAAAAGTACAATGCGCTCCAGCGGTAAGCGGGGAGCCG
GGACAGGAAGGGGTTGACGC

Downstream 100 bases:

>100_bases
GCCACACATCAAGCAGAGAACACAACCCAAGGAGGAGACAACAATGCTGGAAGACGTTAAGAAGGTTCTTGATCTGGTCC
GCCCCGCGCTCCAGGCGGAT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTFRVEKDTLGEVRVPADAYYGAQTVRAVENFPISGLRPHPALVRATVLVKMCAARANMATGRLDSRLGDAIVRAADECL
AGAFDAHFVVDPFQAGAGTSHNMNVNEVLANRANELLGEERGRYAPVHPNDHVNMAQSTNDVFPTAMRLAALRVAGDLRP
ALEGLVAALRKKSAEFDGILKSGRTHLQDAVPIRLGQEFEAWAVALEKNLAAIEGAVPGLAELGIGGTAAGTGMNAEPAY
IDLVVAELARETGFPLVRGANLVERMQNMDPFVALSSALKGLAVNLARIANDLRLLSSGPRTGLAEIALPALQPGSSIMP
GKVNPVMAEVTDMVAFQVMGADTTIMLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPKLADACIAGITADRERCLRYL
EQSVGLATVLAPYIGYAAAAEVAKESTATGRSIRQIVEDRQLLSAGRLAEILDPFPLTSPGVP

Sequences:

>Translated_463_residues
MTFRVEKDTLGEVRVPADAYYGAQTVRAVENFPISGLRPHPALVRATVLVKMCAARANMATGRLDSRLGDAIVRAADECL
AGAFDAHFVVDPFQAGAGTSHNMNVNEVLANRANELLGEERGRYAPVHPNDHVNMAQSTNDVFPTAMRLAALRVAGDLRP
ALEGLVAALRKKSAEFDGILKSGRTHLQDAVPIRLGQEFEAWAVALEKNLAAIEGAVPGLAELGIGGTAAGTGMNAEPAY
IDLVVAELARETGFPLVRGANLVERMQNMDPFVALSSALKGLAVNLARIANDLRLLSSGPRTGLAEIALPALQPGSSIMP
GKVNPVMAEVTDMVAFQVMGADTTIMLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPKLADACIAGITADRERCLRYL
EQSVGLATVLAPYIGYAAAAEVAKESTATGRSIRQIVEDRQLLSAGRLAEILDPFPLTSPGVP
>Mature_462_residues
TFRVEKDTLGEVRVPADAYYGAQTVRAVENFPISGLRPHPALVRATVLVKMCAARANMATGRLDSRLGDAIVRAADECLA
GAFDAHFVVDPFQAGAGTSHNMNVNEVLANRANELLGEERGRYAPVHPNDHVNMAQSTNDVFPTAMRLAALRVAGDLRPA
LEGLVAALRKKSAEFDGILKSGRTHLQDAVPIRLGQEFEAWAVALEKNLAAIEGAVPGLAELGIGGTAAGTGMNAEPAYI
DLVVAELARETGFPLVRGANLVERMQNMDPFVALSSALKGLAVNLARIANDLRLLSSGPRTGLAEIALPALQPGSSIMPG
KVNPVMAEVTDMVAFQVMGADTTIMLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPKLADACIAGITADRERCLRYLE
QSVGLATVLAPYIGYAAAAEVAKESTATGRSIRQIVEDRQLLSAGRLAEILDPFPLTSPGVP

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=44.2764578833693, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=47.1861471861472, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787896, Length=430, Percent_Identity=47.906976744186, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1790398, Length=337, Percent_Identity=27.0029673590504, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=44.8051948051948, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=41.5902140672783, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=42.9805615550756, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=43.6285097192225, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=43.6285097192225, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78710009, Length=439, Percent_Identity=42.1412300683371, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=43.1192660550459, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24583245, Length=438, Percent_Identity=39.4977168949772, Blast_Score=324, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49016; Mature: 48885

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRVEKDTLGEVRVPADAYYGAQTVRAVENFPISGLRPHPALVRATVLVKMCAARANMA
CCEECCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGRLDSRLGDAIVRAADECLAGAFDAHFVVDPFQAGAGTSHNMNVNEVLANRANELLGEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRYAPVHPNDHVNMAQSTNDVFPTAMRLAALRVAGDLRPALEGLVAALRKKSAEFDGIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSGRTHLQDAVPIRLGQEFEAWAVALEKNLAAIEGAVPGLAELGIGGTAAGTGMNAEPAY
HCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHH
IDLVVAELARETGFPLVRGANLVERMQNMDPFVALSSALKGLAVNLARIANDLRLLSSGP
HHHHHHHHHHHCCCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RTGLAEIALPALQPGSSIMPGKVNPVMAEVTDMVAFQVMGADTTIMLAAQAGQLELNVMM
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
PVIAFNLLFSLEILKNTVPKLADACIAGITADRERCLRYLEQSVGLATVLAPYIGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EVAKESTATGRSIRQIVEDRQLLSAGRLAEILDPFPLTSPGVP
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TFRVEKDTLGEVRVPADAYYGAQTVRAVENFPISGLRPHPALVRATVLVKMCAARANMA
CEECCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGRLDSRLGDAIVRAADECLAGAFDAHFVVDPFQAGAGTSHNMNVNEVLANRANELLGEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRYAPVHPNDHVNMAQSTNDVFPTAMRLAALRVAGDLRPALEGLVAALRKKSAEFDGIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSGRTHLQDAVPIRLGQEFEAWAVALEKNLAAIEGAVPGLAELGIGGTAAGTGMNAEPAY
HCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHH
IDLVVAELARETGFPLVRGANLVERMQNMDPFVALSSALKGLAVNLARIANDLRLLSSGP
HHHHHHHHHHHCCCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RTGLAEIALPALQPGSSIMPGKVNPVMAEVTDMVAFQVMGADTTIMLAAQAGQLELNVMM
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
PVIAFNLLFSLEILKNTVPKLADACIAGITADRERCLRYLEQSVGLATVLAPYIGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EVAKESTATGRSIRQIVEDRQLLSAGRLAEILDPFPLTSPGVP
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]