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The map label for this gene is lipA

Identifier: 39995488

GI number: 39995488

Start: 414576

End: 415487

Strand: Direct

Name: lipA

Synonym: GSU0380

Alternate gene names: 39995488

Gene position: 414576-415487 (Clockwise)

Preceding gene: 39995487

Following gene: 39995489

Centisome position: 10.87

GC content: 66.89

Gene sequence:

>912_bases
GTGAACATCCACCGCAAGCCGGAATGGCTCCGGAAAAAAATCAACCCTGCCGCCCACGGCGCCATGGATGAGCTGCTGGG
AGAGTTGCGGCTCCACACGGTCTGCCGGGAGGCGCGCTGCCCCAATATCACCGAGTGCTTCCGGGAACGCCAGGCAACCT
TTCTGATTCTCGGCGCGGAGTGCACCCGCCTCTGCTCCTTCTGCAACGTCACCAAGGGAGAGCCGCTGCCGCCCGACCCG
GACGAGCCGGCACGGGTGGCCCAGGCCGTTGTCCGCCTGAGTCTGGCCCATGTGGTGATCACGAGCCCCACCCGGGACGA
TCTTCCCGACGGCGGAGCCGGCCACTACGTCGCCACCGTGGCGACCATCGGCCGGGTTGCGCCCGCAACCGTCGTGGAGC
TTCTCATCCCCGACTTCCTCGGTAGTCGCGCGGCCCTGGCGGATGTCGTGGCTGCCGCGCCACGGATCATCGGGCACAAT
GTGGAAACCGTTCCCCGTCTCTACGCCATTCGTGCCGGCGCCGACTACGGCCGTTCGCTGGCGGTGCTCCGCACCCTGCG
CGAACTGGCGCCGGGGTGTGCCACCAAGTCGGGGCTCATGCTCGGCCTGGGTGAGACGGAAGAGGAAGTCCTGGCGGTGA
TGGCCGACCTGCGGCGGGTCGACTGCACCTACCTCAGCCTCGGCCAGTACCTGGCTCCGAGCCGGTTCCACCACCCCGTG
CGGGAGTTCGTCCTCCCCGAGACCTTTGACCGGCTGAAGGAACTGGCCGAAAAGATGGGATTCCGCCACGTGGAGAGTGG
CCCCTACGTCCGCAGTTCCTACCACGCTGCCGGCTACGGCGGCGGAACGCGTACAGATCAACCCGTCGCATCCGGCTGTC
TGTCGGATCAGGAAGGAGTATCAGCACAATGA

Upstream 100 bases:

>100_bases
TGGTTCCGTCGGAACTCTCACCGGCGGAGCGGGAGACCGCTGACCGGCTCCTCCGGGAGCGCTACGGCAACGACGACTGG
AATTTCGGGGGAGAAAACGG

Downstream 100 bases:

>100_bases
GAAAGATAACCGTTCTTGCCCTGGTCGCCTCCATGCTCACTCTTGCCGCCTGCTCGGGCAAGGGTGTCCAGGAACTTTAC
GACACAGCCCAGTTCGAGGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNIHRKPEWLRKKINPAAHGAMDELLGELRLHTVCREARCPNITECFRERQATFLILGAECTRLCSFCNVTKGEPLPPDP
DEPARVAQAVVRLSLAHVVITSPTRDDLPDGGAGHYVATVATIGRVAPATVVELLIPDFLGSRAALADVVAAAPRIIGHN
VETVPRLYAIRAGADYGRSLAVLRTLRELAPGCATKSGLMLGLGETEEEVLAVMADLRRVDCTYLSLGQYLAPSRFHHPV
REFVLPETFDRLKELAEKMGFRHVESGPYVRSSYHAAGYGGGTRTDQPVASGCLSDQEGVSAQ

Sequences:

>Translated_303_residues
MNIHRKPEWLRKKINPAAHGAMDELLGELRLHTVCREARCPNITECFRERQATFLILGAECTRLCSFCNVTKGEPLPPDP
DEPARVAQAVVRLSLAHVVITSPTRDDLPDGGAGHYVATVATIGRVAPATVVELLIPDFLGSRAALADVVAAAPRIIGHN
VETVPRLYAIRAGADYGRSLAVLRTLRELAPGCATKSGLMLGLGETEEEVLAVMADLRRVDCTYLSLGQYLAPSRFHHPV
REFVLPETFDRLKELAEKMGFRHVESGPYVRSSYHAAGYGGGTRTDQPVASGCLSDQEGVSAQ
>Mature_303_residues
MNIHRKPEWLRKKINPAAHGAMDELLGELRLHTVCREARCPNITECFRERQATFLILGAECTRLCSFCNVTKGEPLPPDP
DEPARVAQAVVRLSLAHVVITSPTRDDLPDGGAGHYVATVATIGRVAPATVVELLIPDFLGSRAALADVVAAAPRIIGHN
VETVPRLYAIRAGADYGRSLAVLRTLRELAPGCATKSGLMLGLGETEEEVLAVMADLRRVDCTYLSLGQYLAPSRFHHPV
REFVLPETFDRLKELAEKMGFRHVESGPYVRSSYHAAGYGGGTRTDQPVASGCLSDQEGVSAQ

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=41.5492957746479, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=42.0408163265306, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=49.4584837545126, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=40.418118466899, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324770, Length=280, Percent_Identity=42.5, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=40.9090909090909, Blast_Score=205, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_GEOSL (P61196)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951439.1
- ProteinModelPortal:   P61196
- GeneID:   2686621
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0380
- NMPDR:   fig|243231.1.peg.377
- TIGR:   GSU0380
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- ProtClustDB:   PRK05481
- BioCyc:   GSUL243231:GSU_0380-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: NA

EC number: =2.8.1.8

Molecular weight: Translated: 32887; Mature: 32887

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHRKPEWLRKKINPAAHGAMDELLGELRLHTVCREARCPNITECFRERQATFLILGAE
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECHH
CTRLCSFCNVTKGEPLPPDPDEPARVAQAVVRLSLAHVVITSPTRDDLPDGGAGHYVATV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHH
ATIGRVAPATVVELLIPDFLGSRAALADVVAAAPRIIGHNVETVPRLYAIRAGADYGRSL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
AVLRTLRELAPGCATKSGLMLGLGETEEEVLAVMADLRRVDCTYLSLGQYLAPSRFHHPV
HHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REFVLPETFDRLKELAEKMGFRHVESGPYVRSSYHAAGYGGGTRTDQPVASGCLSDQEGV
HHHCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
SAQ
CCC
>Mature Secondary Structure
MNIHRKPEWLRKKINPAAHGAMDELLGELRLHTVCREARCPNITECFRERQATFLILGAE
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECHH
CTRLCSFCNVTKGEPLPPDPDEPARVAQAVVRLSLAHVVITSPTRDDLPDGGAGHYVATV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHH
ATIGRVAPATVVELLIPDFLGSRAALADVVAAAPRIIGHNVETVPRLYAIRAGADYGRSL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
AVLRTLRELAPGCATKSGLMLGLGETEEEVLAVMADLRRVDCTYLSLGQYLAPSRFHHPV
HHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REFVLPETFDRLKELAEKMGFRHVESGPYVRSSYHAAGYGGGTRTDQPVASGCLSDQEGV
HHHCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
SAQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA