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The map label for this gene is gcvH-1 [H]

Identifier: 39995484

GI number: 39995484

Start: 410614

End: 410988

Strand: Direct

Name: gcvH-1 [H]

Synonym: GSU0376

Alternate gene names: 39995484

Gene position: 410614-410988 (Clockwise)

Preceding gene: 39995483

Following gene: 39995485

Centisome position: 10.77

GC content: 60.8

Gene sequence:

>375_bases
ATGGAAACCTATTTCACCAAAGAGCACGAGTGGGTCAAGGTCAAGGAAGGCGTGGCCGCCGTGGGGATCACCGAGTACGC
CGCTCACCAGCTGGGAGACGTTACCTTCGTGGAGCTGCCGCAGGTCGGGAAGCAGGTGAAGCAGTTCGAGGTGCTGGCGG
CCATCGAGTCGGTGAAGGCGGCCAGTGACATCTACGCCCCGGTTTCGGGGAAGGTGACCCAGGTGAACGAAGCCCTTGAC
GATCGTCCCGAGATCGTGAACGAGGCCGCCGAGGAGGCGGGGTGGATTGCCTGGATCGAGATGGGGGATACCTCTGAACT
GAACGGTCTCCTGACCCGCGAACAGTACGACGAGTATCTGAAGGGCCTCGAATAA

Upstream 100 bases:

>100_bases
GTGGTGGACGCCCCTTTCTATCGGGGAGGGTCCCTGCGCGCCTGATTTGCCGGCAGATAATCCATTCAGTTCGTCATACA
TCCACAAGGAGGCAACAGTC

Downstream 100 bases:

>100_bases
TGAGCGGCGCCGACTACTGCCCCAACTCGCCGGAGGAGGTCCGCCGGATGCTGGACGCTGTCGGCGTCGCCGACATTGAT
GACCTTTTCCGCCCGATTTC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
METYFTKEHEWVKVKEGVAAVGITEYAAHQLGDVTFVELPQVGKQVKQFEVLAAIESVKAASDIYAPVSGKVTQVNEALD
DRPEIVNEAAEEAGWIAWIEMGDTSELNGLLTREQYDEYLKGLE

Sequences:

>Translated_124_residues
METYFTKEHEWVKVKEGVAAVGITEYAAHQLGDVTFVELPQVGKQVKQFEVLAAIESVKAASDIYAPVSGKVTQVNEALD
DRPEIVNEAAEEAGWIAWIEMGDTSELNGLLTREQYDEYLKGLE
>Mature_124_residues
METYFTKEHEWVKVKEGVAAVGITEYAAHQLGDVTFVELPQVGKQVKQFEVLAAIESVKAASDIYAPVSGKVTQVNEALD
DRPEIVNEAAEEAGWIAWIEMGDTSELNGLLTREQYDEYLKGLE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=43.3333333333333, Blast_Score=120, Evalue=2e-28,
Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=43.3333333333333, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=50.4132231404959, Blast_Score=112, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=49.5652173913044, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=47.8632478632479, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=43.3333333333333, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=45, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13776; Mature: 13776

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYFTKEHEWVKVKEGVAAVGITEYAAHQLGDVTFVELPQVGKQVKQFEVLAAIESVKA
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGKVTQVNEALDDRPEIVNEAAEEAGWIAWIEMGDTSELNGLLTREQYDEYL
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
KGLE
HCCC
>Mature Secondary Structure
METYFTKEHEWVKVKEGVAAVGITEYAAHQLGDVTFVELPQVGKQVKQFEVLAAIESVKA
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGKVTQVNEALDDRPEIVNEAAEEAGWIAWIEMGDTSELNGLLTREQYDEYL
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
KGLE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]