Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 39995249

GI number: 39995249

Start: 152504

End: 154084

Strand: Direct

Name: prfC [H]

Synonym: GSU0138

Alternate gene names: 39995249

Gene position: 152504-154084 (Clockwise)

Preceding gene: 39995247

Following gene: 39995250

Centisome position: 4.0

GC content: 59.9

Gene sequence:

>1581_bases
ATGAAGTACAACGAACAGGAAGTCGATCGTCGGCGGACCTTTGCCATTGTCAGTCATCCGGACGCCGGTAAAACCACCAT
CACCGAAAAACTGCTGCTGTTTGGCGGCGCCATCCAGCAGGCGGGCGAGGTCCGTGCCCGCAAGGCGGCCCGCCACGCCA
CCTCGGACTGGATGGAGATGGAAAAGCAGCGCGGCATCTCCGTCACCTCGTCGGTCATGAAATTCACCTACCGGGATTAC
GAAGTGAACCTGCTGGACACTCCCGGTCACAATGACTTTTCAGAGGACACCTATCGGGTCCTTACCGCCGTCGACTCGGC
CCTGATGGTCATCGATTCGGTGAAGGGCGTTGAGAGCCAGACCATCAAGCTCCTGGATGTGTGTCGCCTGCGCCACACGC
CGATCATGACCTTCATCAACAAGCTTGACCGTGAAGGTCGGGATCCTTTTGAGCTTATCGACGAAATCGAAAAAGTCTTG
AGAATCCAGTGTGCCCCCATGACGTGGCCCATCGGCATGGGCAAGCGCTTCCGGGGCACCTATCACCTCTACACCAAAGA
GCTCGTTATCTTCGATGCCGAGGCGGAGCGCGGAACTGGCGGCGTTATCTCCATGACCGGTCTCGACGACCCGCAGTTGG
ACGAACTCCTGGGCTCCCAGGCCGACGAGTTGCGTGCCGATGTGGAGCTGCTGGAAGGGGCCGCCCATCCCTTCGAGGAA
GAGGCGTATCATGCCGGGCTCCAGACCCCGGTCTTCTTCGGTAGCGCCATTAATACATTCGGCGTGCAGCAACTTCTCGA
CACCTTTGTGGATCATGCCCCGGCGCCGCTTCCCCGTGAGGCGGTCAGCCGCACCGTTTCCCCCTATGAAGAGCCCTTCA
CTGCCTTCGCCTTCAAGATCCAGGCGAATATGGACCCCGCCCACCGGGACCGGATCGCCTTTTTCCGGATATGTTCCGGC
AAATTCACCCGGGGCATGAAGGTCCGCCACGTGCGGCTCGGTCGCGAGGTGGCCATCAACAATGCAACCATCTTCATGGC
CCAGGACCGCACCCATGTGGACGAGGCGTTTCCCGGCGACATCATCGGCATTCACAACCACGGCACCATCAAGATCGGCG
ACACCTTCACCCTGGGGGAGGATATCAAGTTCACGGGCATTCCCAACTTTGCCCCTGAACACTTCCGCCGGGTGCGCCTG
CTGGATCCCCTGAAATCCAAGGCCCTGGAAAAGGGGCTGACCCAGTTGGCCGAAGAGGGGACCACCCAGGTCTTTCGCCC
GCTCATGGGTGCCGACTGGGTTGTCGGAGCCGTCGGTCTCCTCCAGTTCGACGTGGTCATGCACCGGCTCGAGCACGAGT
ATAACGTGAAGGCCACCTATGAGCCGGTTTCCTACGTGACCGCCCGCTGGGTGACCGGGGAGAAGAAGAAGGTCGAGGAA
TTCCAGAAGAAGGAAGTGATGAACTGTTACATCGACGGCGAGGGGGATCTGGCGTATCTGGCCGGCTCCCAATGGCGCCT
CGACAACACCATGGACAATTGGAAAGACCTCACCTTCCACGCCACCCGTGAACACAGCTGA

Upstream 100 bases:

>100_bases
ACCGTCGGGGTGCTGGTGTATTATTGATTTTCAAGGCGAAGAGTAGCTTCTTCGCCTTTTCTTTGTCCAGATATTTTCAA
GCCTTTCGGAGCTCCACGCA

Downstream 100 bases:

>100_bases
TTACCTAAGCACAATGTGTGTCACTTATGGCTCATGCTGTGACGGGTCTGACACAAAATGCAGAGGGCATAATTGGCACA
TAATGTGTAATCCCTCTGTA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKYNEQEVDRRRTFAIVSHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRDY
EVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDSVKGVESQTIKLLDVCRLRHTPIMTFINKLDREGRDPFELIDEIEKVL
RIQCAPMTWPIGMGKRFRGTYHLYTKELVIFDAEAERGTGGVISMTGLDDPQLDELLGSQADELRADVELLEGAAHPFEE
EAYHAGLQTPVFFGSAINTFGVQQLLDTFVDHAPAPLPREAVSRTVSPYEEPFTAFAFKIQANMDPAHRDRIAFFRICSG
KFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTLGEDIKFTGIPNFAPEHFRRVRL
LDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWVVGAVGLLQFDVVMHRLEHEYNVKATYEPVSYVTARWVTGEKKKVEE
FQKKEVMNCYIDGEGDLAYLAGSQWRLDNTMDNWKDLTFHATREHS

Sequences:

>Translated_526_residues
MKYNEQEVDRRRTFAIVSHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRDY
EVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDSVKGVESQTIKLLDVCRLRHTPIMTFINKLDREGRDPFELIDEIEKVL
RIQCAPMTWPIGMGKRFRGTYHLYTKELVIFDAEAERGTGGVISMTGLDDPQLDELLGSQADELRADVELLEGAAHPFEE
EAYHAGLQTPVFFGSAINTFGVQQLLDTFVDHAPAPLPREAVSRTVSPYEEPFTAFAFKIQANMDPAHRDRIAFFRICSG
KFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTLGEDIKFTGIPNFAPEHFRRVRL
LDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWVVGAVGLLQFDVVMHRLEHEYNVKATYEPVSYVTARWVTGEKKKVEE
FQKKEVMNCYIDGEGDLAYLAGSQWRLDNTMDNWKDLTFHATREHS
>Mature_526_residues
MKYNEQEVDRRRTFAIVSHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRDY
EVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDSVKGVESQTIKLLDVCRLRHTPIMTFINKLDREGRDPFELIDEIEKVL
RIQCAPMTWPIGMGKRFRGTYHLYTKELVIFDAEAERGTGGVISMTGLDDPQLDELLGSQADELRADVELLEGAAHPFEE
EAYHAGLQTPVFFGSAINTFGVQQLLDTFVDHAPAPLPREAVSRTVSPYEEPFTAFAFKIQANMDPAHRDRIAFFRICSG
KFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTLGEDIKFTGIPNFAPEHFRRVRL
LDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWVVGAVGLLQFDVVMHRLEHEYNVKATYEPVSYVTARWVTGEKKKVEE
FQKKEVMNCYIDGEGDLAYLAGSQWRLDNTMDNWKDLTFHATREHS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=496, Percent_Identity=27.0161290322581, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI19923640, Length=507, Percent_Identity=25.2465483234714, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.4809160305344, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI25306287, Length=308, Percent_Identity=29.8701298701299, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=31.3953488372093, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=59.9616858237548, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=510, Percent_Identity=28.6274509803922, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.2281879194631, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=31.4102564102564, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=504, Percent_Identity=27.7777777777778, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=166, Percent_Identity=32.5301204819277, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=140, Percent_Identity=34.2857142857143, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=494, Percent_Identity=28.5425101214575, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=416, Percent_Identity=28.125, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=30, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=30, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=28.476821192053, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=32.8859060402685, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24582462, Length=502, Percent_Identity=27.8884462151394, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI221458488, Length=508, Percent_Identity=24.4094488188976, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.8701298701299, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.8701298701299, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.8701298701299, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59537; Mature: 59537

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYNEQEVDRRRTFAIVSHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEM
CCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKQRGISVTSSVMKFTYRDYEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDSVKGVESQ
HHHCCCHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TIKLLDVCRLRHTPIMTFINKLDREGRDPFELIDEIEKVLRIQCAPMTWPIGMGKRFRGT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
YHLYTKELVIFDAEAERGTGGVISMTGLDDPQLDELLGSQADELRADVELLEGAAHPFEE
EEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHH
EAYHAGLQTPVFFGSAINTFGVQQLLDTFVDHAPAPLPREAVSRTVSPYEEPFTAFAFKI
HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEEEE
QANMDPAHRDRIAFFRICSGKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGD
ECCCCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCCCCCCCC
IIGIHNHGTIKIGDTFTLGEDIKFTGIPNFAPEHFRRVRLLDPLKSKALEKGLTQLAEEG
EEEEECCCEEEECCEEECCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
TTQVFRPLMGADWVVGAVGLLQFDVVMHRLEHEYNVKATYEPVSYVTARWVTGEKKKVEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEEEECCCCHHHHHH
FQKKEVMNCYIDGEGDLAYLAGSQWRLDNTMDNWKDLTFHATREHS
HHHHHHEEEEECCCCCEEEEECCCEEECCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MKYNEQEVDRRRTFAIVSHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEM
CCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKQRGISVTSSVMKFTYRDYEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDSVKGVESQ
HHHCCCHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TIKLLDVCRLRHTPIMTFINKLDREGRDPFELIDEIEKVLRIQCAPMTWPIGMGKRFRGT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
YHLYTKELVIFDAEAERGTGGVISMTGLDDPQLDELLGSQADELRADVELLEGAAHPFEE
EEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHH
EAYHAGLQTPVFFGSAINTFGVQQLLDTFVDHAPAPLPREAVSRTVSPYEEPFTAFAFKI
HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEEEE
QANMDPAHRDRIAFFRICSGKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGD
ECCCCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCCCCCCCC
IIGIHNHGTIKIGDTFTLGEDIKFTGIPNFAPEHFRRVRLLDPLKSKALEKGLTQLAEEG
EEEEECCCEEEECCEEECCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
TTQVFRPLMGADWVVGAVGLLQFDVVMHRLEHEYNVKATYEPVSYVTARWVTGEKKKVEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEEEECCCCHHHHHH
FQKKEVMNCYIDGEGDLAYLAGSQWRLDNTMDNWKDLTFHATREHS
HHHHHHEEEEECCCCCEEEEECCCEEECCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA