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The map label for this gene is nrfC [H]

Identifier: 39995180

GI number: 39995180

Start: 84481

End: 85254

Strand: Direct

Name: nrfC [H]

Synonym: GSU0069

Alternate gene names: 39995180

Gene position: 84481-85254 (Clockwise)

Preceding gene: 39995179

Following gene: 39995181

Centisome position: 2.21

GC content: 61.63

Gene sequence:

>774_bases
ATGAATAGACGCGAGTTTCTCAGACGGAGCATGGTGGTTGTCGCGGGGTTGGCGGTGCCGGCGGCGGCTCTGGAGCTTGT
CGACCCGAAAAGGCTGCTGGCGGCACGGCCGGAGTTGCGCTGGGCCTTTCTGGTTGATACCTACAAATGCGTGGGGTGTG
GCTTCTGTGTCAAGGCGTGCAAGGTGGAGAACGAGGTCCCCTACGAGGCCAACGTGACTCGTACGTGGGTCGAGCGTTAC
GTGGTGAAACCGGATGGCGAGGTTCTCATTGATTCGCCACGTGGCGCCCGCGACGGTTTCACCGATAAGAGAATCGAGGT
CGGAACGGGGAAACCCCGGGACGTGGGGGATGAAGAGGTGGAAAAAGCCTTCTTTGTCCCAAAACTCTGCAATCAGTGCG
ACAACCCGCCCTGCGTCCAGGTCTGCCCCGTGGGGGCCACCTACAAGACCGTCGACGGGGTCGTGCTCGTGGACCGCTCA
TGGTGCATCGGCTGCGGCTATTGCATCATGGGGTGCCCGTACGGGGTCCGTTTTTTTCATCCGGTGCACAAGGTGGCGGA
GAAGTGCAACTTCTGTTACCACCGGATTTCCCAGGGGCAGGCGACGGCGTGCGTCGACGCCTGCCCGTTCGGCGCCCGCC
GGATCGGCAATCTTCGCGATCCGCATGACCCGATCACCAAGATCATCATGGCCGGGCGGGTCGGCATCCTCAAGGAAGAG
TACGGAACCAAGCCCCAGGTCTACTATGTGGGGCTCTCCAAGGAGGTACGCTAG

Upstream 100 bases:

>100_bases
TCGCAGATTCGCGGCATAGATCCCGATACACACAACCCCCAGGCCGAGTGCCGGCTCTGCCACGATCCCCACCACCCAAA
TCTGGAGGAGTTGAAGCGCT

Downstream 100 bases:

>100_bases
CCATGGTGCACGGGGAGGCCTGGACCGTCAAAGAGCTGTTCGTCTATCCCAACGAGTACATCTACTGGACCATTCAGATC
GTCATGTACCCGTTCCTGAC

Product: oxidoreductase, iron-sulfur cluster-binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNRREFLRRSMVVVAGLAVPAAALELVDPKRLLAARPELRWAFLVDTYKCVGCGFCVKACKVENEVPYEANVTRTWVERY
VVKPDGEVLIDSPRGARDGFTDKRIEVGTGKPRDVGDEEVEKAFFVPKLCNQCDNPPCVQVCPVGATYKTVDGVVLVDRS
WCIGCGYCIMGCPYGVRFFHPVHKVAEKCNFCYHRISQGQATACVDACPFGARRIGNLRDPHDPITKIIMAGRVGILKEE
YGTKPQVYYVGLSKEVR

Sequences:

>Translated_257_residues
MNRREFLRRSMVVVAGLAVPAAALELVDPKRLLAARPELRWAFLVDTYKCVGCGFCVKACKVENEVPYEANVTRTWVERY
VVKPDGEVLIDSPRGARDGFTDKRIEVGTGKPRDVGDEEVEKAFFVPKLCNQCDNPPCVQVCPVGATYKTVDGVVLVDRS
WCIGCGYCIMGCPYGVRFFHPVHKVAEKCNFCYHRISQGQATACVDACPFGARRIGNLRDPHDPITKIIMAGRVGILKEE
YGTKPQVYYVGLSKEVR
>Mature_257_residues
MNRREFLRRSMVVVAGLAVPAAALELVDPKRLLAARPELRWAFLVDTYKCVGCGFCVKACKVENEVPYEANVTRTWVERY
VVKPDGEVLIDSPRGARDGFTDKRIEVGTGKPRDVGDEEVEKAFFVPKLCNQCDNPPCVQVCPVGATYKTVDGVVLVDRS
WCIGCGYCIMGCPYGVRFFHPVHKVAEKCNFCYHRISQGQATACVDACPFGARRIGNLRDPHDPITKIIMAGRVGILKEE
YGTKPQVYYVGLSKEVR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=214, Percent_Identity=35.981308411215, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI226510944, Length=220, Percent_Identity=32.2727272727273, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787872, Length=211, Percent_Identity=29.8578199052133, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787122, Length=211, Percent_Identity=29.3838862559242, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787478, Length=160, Percent_Identity=36.25, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787740, Length=119, Percent_Identity=41.1764705882353, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789370, Length=230, Percent_Identity=29.5652173913043, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790326, Length=198, Percent_Identity=28.7878787878788, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=202, Percent_Identity=27.2277227722772, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082180, Length=89, Percent_Identity=42.6966292134831, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787963, Length=227, Percent_Identity=25.9911894273128, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082179, Length=102, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367245, Length=85, Percent_Identity=40, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28641; Mature: 28641

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFLRRSMVVVAGLAVPAAALELVDPKRLLAARPELRWAFLVDTYKCVGCGFCVKAC
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
KVENEVPYEANVTRTWVERYVVKPDGEVLIDSPRGARDGFTDKRIEVGTGKPRDVGDEEV
HCCCCCCCCCCCHHHHHHHEEECCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHH
EKAFFVPKLCNQCDNPPCVQVCPVGATYKTVDGVVLVDRSWCIGCGYCIMGCPYGVRFFH
HHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCEEEEECCCHHCCCHHHHCCCCCHHHHH
PVHKVAEKCNFCYHRISQGQATACVDACPFGARRIGNLRDPHDPITKIIMAGRVGILKEE
HHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCHHHH
YGTKPQVYYVGLSKEVR
HCCCCEEEEEECCCCCC
>Mature Secondary Structure
MNRREFLRRSMVVVAGLAVPAAALELVDPKRLLAARPELRWAFLVDTYKCVGCGFCVKAC
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
KVENEVPYEANVTRTWVERYVVKPDGEVLIDSPRGARDGFTDKRIEVGTGKPRDVGDEEV
HCCCCCCCCCCCHHHHHHHEEECCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHH
EKAFFVPKLCNQCDNPPCVQVCPVGATYKTVDGVVLVDRSWCIGCGYCIMGCPYGVRFFH
HHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCEEEEECCCHHCCCHHHHCCCCCHHHHH
PVHKVAEKCNFCYHRISQGQATACVDACPFGARRIGNLRDPHDPITKIIMAGRVGILKEE
HHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCHHHH
YGTKPQVYYVGLSKEVR
HCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]