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The map label for this gene is tolB

Identifier: 39995136

GI number: 39995136

Start: 35287

End: 36585

Strand: Reverse

Name: tolB

Synonym: GSU0025

Alternate gene names: 39995136

Gene position: 36585-35287 (Counterclockwise)

Preceding gene: 39995137

Following gene: 39995135

Centisome position: 0.96

GC content: 61.05

Gene sequence:

>1299_bases
ATGAAACACGTAAGAATCTTTGCCACGCTCCTTGCCCTGCTGGTCATCTCCGTCACGCCGGCGGTGATCCATTCCGAGGA
CGTCTACCGCGAGGTGACCGCCTCGGGGGCCCACAACCTCACCCTTGCCGTCGACAATCCACGGAATCTCGGCGGTGCCG
ACGATGCCGCCCTGGCCCGTGATGTGGCCGAGGTGCTCAGATTCGACATGACCTTGGCGGGCCCCTTCAGCGTAATGGCT
CCGCCGGCGGGCACTTCGCCGGGAGGGATTCGTCCCGGCGAATTTGACTTGGACTCCTGGAGAAATGCCGGCGTCGACCT
GCTGGTCAAAAGCGGCTACACCATCACGGGGGACTCCGTTACCATGGAGTTCCGCCTCTACAACGCCACCCAGGGGCGCG
AGCTCGCCGCCAAGCGCTATACCGGCAAACGCTCGGATCTGCGACGGATCACCCACACCTTTTCCGATGACATCATGCAG
ACGATGACTGGGGAGAGGGGTCCTTTCACGGGCAAGATTGCCTTTGTCTCCACCGAGAGCGGCAACAAGGAAATCTACCT
GATGGACTACGATGGGCATAACGTACAGCGTCTCACGAAAAACCGCTCCATCAACCTGAACCCCGATTTTTCCCCCAACG
GCCGTGAGCTCGCCTATACCTCCTATCGCCGCGGCAATCCGGATCTTTTTCGACGCGAAATATTCACCGGAACGGAAGCG
GTGGTATCGTCACACCGGGGCATCAACGTTACCGGAACCTGGTCCCCGGACGGCAAACAACTGGCCCTTGCCATGAGCAG
GGACGGCAATTCCGAAATCTACGCTATTTCCCGTGACGGCAGGGACCCCAGGCGGCTCACCACCCATCAGGCAATAGATG
TTTCACCCGCCTGGTCCCCGGATGGCAAGCGGATCGCTTTTGTCTCGGACCGGCTCGGCAAGCCCCAGGTATTCATCATG
AACGCCGACGGCTCCGACGTACGGCGACTCACAACCAGCGGCGCCTATAATGTCTCGCCCCGCTGGTCGCCCAAGGGGGA
CAGACTCGTCTACTGTCGTCAGGAAGGGGGCTTCCAGATCTACTCCATTGCGACGGACGGAACCGGTGACACCCGCCTTA
CCAGCGAAGGGAGCAACGAGCATCCCCGTTGGTCCCCGGACGGGCGATTTCTGACGTTCAGTTCCACCCGCGATGGAGGC
GAAGCCATCTACGTCATGCGTTCCGACGGCAGCGGCCAGACGCGGGTCTACCGAAGCAAGGGAAAAGCGTCCCACCCAAC
TTGGTCGCCACGGTGGTAA

Upstream 100 bases:

>100_bases
AAGCCATCGCCATGGCGGAGAAAACGTTCGCTCCGCCGCCGTCCGGGGGGGAGTTTGAAACCGGTTTCATCTTCAGGCCG
GAGGGGGTAGGGAAGAGATA

Downstream 100 bases:

>100_bases
GACATCGTTTTTACTTGCAGTTACCATCTAACCCATTGAAAAAGCATCTCCATTATATATAATTGTTCGCGTTGACGCGG
CGCGGGTCGTACCCGCCCGA

Product: tolB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKHVRIFATLLALLVISVTPAVIHSEDVYREVTASGAHNLTLAVDNPRNLGGADDAALARDVAEVLRFDMTLAGPFSVMA
PPAGTSPGGIRPGEFDLDSWRNAGVDLLVKSGYTITGDSVTMEFRLYNATQGRELAAKRYTGKRSDLRRITHTFSDDIMQ
TMTGERGPFTGKIAFVSTESGNKEIYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEA
VVSSHRGINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSPDGKRIAFVSDRLGKPQVFIM
NADGSDVRRLTTSGAYNVSPRWSPKGDRLVYCRQEGGFQIYSIATDGTGDTRLTSEGSNEHPRWSPDGRFLTFSSTRDGG
EAIYVMRSDGSGQTRVYRSKGKASHPTWSPRW

Sequences:

>Translated_432_residues
MKHVRIFATLLALLVISVTPAVIHSEDVYREVTASGAHNLTLAVDNPRNLGGADDAALARDVAEVLRFDMTLAGPFSVMA
PPAGTSPGGIRPGEFDLDSWRNAGVDLLVKSGYTITGDSVTMEFRLYNATQGRELAAKRYTGKRSDLRRITHTFSDDIMQ
TMTGERGPFTGKIAFVSTESGNKEIYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEA
VVSSHRGINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSPDGKRIAFVSDRLGKPQVFIM
NADGSDVRRLTTSGAYNVSPRWSPKGDRLVYCRQEGGFQIYSIATDGTGDTRLTSEGSNEHPRWSPDGRFLTFSSTRDGG
EAIYVMRSDGSGQTRVYRSKGKASHPTWSPRW
>Mature_432_residues
MKHVRIFATLLALLVISVTPAVIHSEDVYREVTASGAHNLTLAVDNPRNLGGADDAALARDVAEVLRFDMTLAGPFSVMA
PPAGTSPGGIRPGEFDLDSWRNAGVDLLVKSGYTITGDSVTMEFRLYNATQGRELAAKRYTGKRSDLRRITHTFSDDIMQ
TMTGERGPFTGKIAFVSTESGNKEIYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEA
VVSSHRGINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSPDGKRIAFVSDRLGKPQVFIM
NADGSDVRRLTTSGAYNVSPRWSPKGDRLVYCRQEGGFQIYSIATDGTGDTRLTSEGSNEHPRWSPDGRFLTFSSTRDGG
EAIYVMRSDGSGQTRVYRSKGKASHPTWSPRW

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=426, Percent_Identity=28.169014084507, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32564215, Length=217, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI32564217, Length=217, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_GEOSL (Q74H67)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951087.1
- ProteinModelPortal:   Q74H67
- SMR:   Q74H67
- GeneID:   2688562
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0025
- NMPDR:   fig|243231.1.peg.25
- TIGR:   GSU0025
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   CLSK827594
- BioCyc:   GSUL243231:GSU_0025-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 47672; Mature: 47672

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVRIFATLLALLVISVTPAVIHSEDVYREVTASGAHNLTLAVDNPRNLGGADDAALAR
CCHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHH
DVAEVLRFDMTLAGPFSVMAPPAGTSPGGIRPGEFDLDSWRNAGVDLLVKSGYTITGDSV
HHHHHHHHCEEECCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCEEECCCE
TMEFRLYNATQGRELAAKRYTGKRSDLRRITHTFSDDIMQTMTGERGPFTGKIAFVSTES
EEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
GNKEIYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEA
CCCEEEEEECCCCCHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHH
VVSSHRGINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSP
HHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCC
DGKRIAFVSDRLGKPQVFIMNADGSDVRRLTTSGAYNVSPRWSPKGDRLVYCRQEGGFQI
CCCEEEEEECCCCCCEEEEECCCCCCHHEEECCCCEECCCCCCCCCCEEEEEEECCCEEE
YSIATDGTGDTRLTSEGSNEHPRWSPDGRFLTFSSTRDGGEAIYVMRSDGSGQTRVYRSK
EEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECC
GKASHPTWSPRW
CCCCCCCCCCCC
>Mature Secondary Structure
MKHVRIFATLLALLVISVTPAVIHSEDVYREVTASGAHNLTLAVDNPRNLGGADDAALAR
CCHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHH
DVAEVLRFDMTLAGPFSVMAPPAGTSPGGIRPGEFDLDSWRNAGVDLLVKSGYTITGDSV
HHHHHHHHCEEECCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCEEECCCE
TMEFRLYNATQGRELAAKRYTGKRSDLRRITHTFSDDIMQTMTGERGPFTGKIAFVSTES
EEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
GNKEIYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEA
CCCEEEEEECCCCCHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHH
VVSSHRGINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSP
HHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCC
DGKRIAFVSDRLGKPQVFIMNADGSDVRRLTTSGAYNVSPRWSPKGDRLVYCRQEGGFQI
CCCEEEEEECCCCCCEEEEECCCCCCHHEEECCCCEECCCCCCCCCCEEEEEEECCCEEE
YSIATDGTGDTRLTSEGSNEHPRWSPDGRFLTFSSTRDGGEAIYVMRSDGSGQTRVYRSK
EEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECC
GKASHPTWSPRW
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA