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The map label for this gene is otsA [H]

Identifier: 308513316

GI number: 308513316

Start: 2555335

End: 2556795

Strand: Reverse

Name: otsA [H]

Synonym: NA

Alternate gene names: 308513316

Gene position: 2556795-2555335 (Counterclockwise)

Preceding gene: 39997435

Following gene: 39997433

Centisome position: 67.03

GC content: 65.43

Gene sequence:

>1461_bases
GTGGGGGAGCGGATACTGGTCGTTTCCAACCGCGAACCCTATATCCATATCCGCACGGGCGACACCGTGGAGGTCCATCG
TCCGGCCAGCGGCCTGGTCACCGCCGTGGAGCCGGTCATGCGTGCCTGCTCGGGAACCTGGATCGCCCATGGCAGCGGCT
CCGCCGACCGGGAGACGGTCGACTCCCACGACCGGGTCCGGGTGCCGCCCCACAAGCCTGAATACAATCTGCGACGGATC
TGGCTCACCAAGGAAGAGGAAGAGGGGTACTACTACGGCTTCGCCAACGAAGGGCTCTGGCCCCTCTGCCATATCGCCCA
CGTACGCCCGGTGTTCCGCACCAGCGACTGGCAACAGTACGTCAGGATCAACCAGCGGTTCGCCGACGCCGTGGTCAAGG
AGGCGGACAGCGACGATCCGGTTGTGCTGGTACAGGACTACCACTTCGCGCTTCTGCCCGCGATGATCCGCAAGGCCCTG
CCCAAGGCCACCATCATCACCTTCTGGCACATTCCGTGGCCCAACCCGGAGTCGTTCGGCATCTGCCCCTGGCGGGAGGA
ACTGCTCAAGGGGATGCTGGGGAGCACCATTCTCGGCTTCCACACCCCGTACCATTGCAAGAACTTCCTGGAGACGGTGG
ACCGCTACCTGGAGACCCGGATCGAGCATGAATCGTCCACCATCTCCCGGCGAGGCCAATTGACCATGGTTGAGAGTTAC
CCAATCTCCATCCAGTGGCCGCCGGCCTGGCAGGGGACCCTGCCGCCGGTGGACGTGACGCGGAGCGAAATCCTTGGCGA
ACTGGGGCTTCCCCTGGATCACCTGATCGGGGTCGGGGTTGACCGGATGGATTATACCAAGGGAATCCCGGAGCGATTCA
TGGCGGTGGAGCGGCTGTTCGAACTGCACCCCGAACTGGTGGGACGCTTCACCTTTGTCCAGATCGCCGCGCCGAGCCGG
TCGGCCCTGGAGGAGTACCAGGCGTTCGAGGCGAGGGTCCATTCCCACGCGGAGCGGATCAACGGGCGTTTCAGCCGCGA
CGGGCGCCCGGCCATCATCCTCAAGGCCGAGCACCACGAACCCGAGCAGGTCAACCGTTACTACCGCGCCGCCGATGTCT
GCATGGTCACCAGCCTCCACGACGGCATGAACCTGGTGGCCAAGGAGTACGTGGCGGCCCGGGACGACGAGCGGGGCGCC
CTGGTGCTGAGCCAGTTCACCGGCGCTGCCCACGAACTGCATGAGGCCCTGATCGTCAACCCCTATCATGTGGAACAGAC
CGCCGAGGCCCTCCACCGGGCCCTCACCATGCCCGATTACGAGCAGCGGGAACGGATGCGGAGCATGCGCATGCTGGTGC
GCGATTTCAACGTCTACCGCTGGGCCGGCCGGATGCTGCTGGACGCGGCCAGGGTCCGCCAGCGGGAGAAACTGGCCGCC
CGGATCGGGGGCGAGTCGTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: glycosyl transferase, group 20 family protein

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:
NA

Sequences:

>Translated_486_residues
MGERILVVSNREPYIHIRTGDTVEVHRPASGLVTAVEPVMRACSGTWIAHGSGSADRETVDSHDRVRVPPHKPEYNLRRI
WLTKEEEEGYYYGFANEGLWPLCHIAHVRPVFRTSDWQQYVRINQRFADAVVKEADSDDPVVLVQDYHFALLPAMIRKAL
PKATIITFWHIPWPNPESFGICPWREELLKGMLGSTILGFHTPYHCKNFLETVDRYLETRIEHESSTISRRGQLTMVESY
PISIQWPPAWQGTLPPVDVTRSEILGELGLPLDHLIGVGVDRMDYTKGIPERFMAVERLFELHPELVGRFTFVQIAAPSR
SALEEYQAFEARVHSHAERINGRFSRDGRPAIILKAEHHEPEQVNRYYRAADVCMVTSLHDGMNLVAKEYVAARDDERGA
LVLSQFTGAAHELHEALIVNPYHVEQTAEALHRALTMPDYEQRERMRSMRMLVRDFNVYRWAGRMLLDAARVRQREKLAA
RIGGES
>Mature_485_residues
GERILVVSNREPYIHIRTGDTVEVHRPASGLVTAVEPVMRACSGTWIAHGSGSADRETVDSHDRVRVPPHKPEYNLRRIW
LTKEEEEGYYYGFANEGLWPLCHIAHVRPVFRTSDWQQYVRINQRFADAVVKEADSDDPVVLVQDYHFALLPAMIRKALP
KATIITFWHIPWPNPESFGICPWREELLKGMLGSTILGFHTPYHCKNFLETVDRYLETRIEHESSTISRRGQLTMVESYP
ISIQWPPAWQGTLPPVDVTRSEILGELGLPLDHLIGVGVDRMDYTKGIPERFMAVERLFELHPELVGRFTFVQIAAPSRS
ALEEYQAFEARVHSHAERINGRFSRDGRPAIILKAEHHEPEQVNRYYRAADVCMVTSLHDGMNLVAKEYVAARDDERGAL
VLSQFTGAAHELHEALIVNPYHVEQTAEALHRALTMPDYEQRERMRSMRMLVRDFNVYRWAGRMLLDAARVRQREKLAAR
IGGES

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=489, Percent_Identity=33.5378323108384, Blast_Score=219, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI115533560, Length=507, Percent_Identity=24.8520710059172, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI115533558, Length=507, Percent_Identity=24.8520710059172, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71987012, Length=430, Percent_Identity=26.5116279069767, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319602, Length=469, Percent_Identity=33.4754797441365, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323537, Length=407, Percent_Identity=27.7641277641278, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6323917, Length=389, Percent_Identity=25.706940874036, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320279, Length=398, Percent_Identity=24.8743718592965, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920676, Length=502, Percent_Identity=34.2629482071713, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 55813; Mature: 55682

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERILVVSNREPYIHIRTGDTVEVHRPASGLVTAVEPVMRACSGTWIAHGSGSADRETV
CCCEEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHC
DSHDRVRVPPHKPEYNLRRIWLTKEEEEGYYYGFANEGLWPLCHIAHVRPVFRTSDWQQY
CCCCCEECCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCHHHCCCCHHHH
VRINQRFADAVVKEADSDDPVVLVQDYHFALLPAMIRKALPKATIITFWHIPWPNPESFG
HHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
ICPWREELLKGMLGSTILGFHTPYHCKNFLETVDRYLETRIEHESSTISRRGQLTMVESY
CCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PISIQWPPAWQGTLPPVDVTRSEILGELGLPLDHLIGVGVDRMDYTKGIPERFMAVERLF
CEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
ELHPELVGRFTFVQIAAPSRSALEEYQAFEARVHSHAERINGRFSRDGRPAIILKAEHHE
HHCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
PEQVNRYYRAADVCMVTSLHDGMNLVAKEYVAARDDERGALVLSQFTGAAHELHEALIVN
HHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHCCC
PYHVEQTAEALHRALTMPDYEQRERMRSMRMLVRDFNVYRWAGRMLLDAARVRQREKLAA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIGGES
HCCCCC
>Mature Secondary Structure 
GERILVVSNREPYIHIRTGDTVEVHRPASGLVTAVEPVMRACSGTWIAHGSGSADRETV
CCEEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHC
DSHDRVRVPPHKPEYNLRRIWLTKEEEEGYYYGFANEGLWPLCHIAHVRPVFRTSDWQQY
CCCCCEECCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCHHHCCCCHHHH
VRINQRFADAVVKEADSDDPVVLVQDYHFALLPAMIRKALPKATIITFWHIPWPNPESFG
HHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
ICPWREELLKGMLGSTILGFHTPYHCKNFLETVDRYLETRIEHESSTISRRGQLTMVESY
CCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PISIQWPPAWQGTLPPVDVTRSEILGELGLPLDHLIGVGVDRMDYTKGIPERFMAVERLF
CEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
ELHPELVGRFTFVQIAAPSRSALEEYQAFEARVHSHAERINGRFSRDGRPAIILKAEHHE
HHCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
PEQVNRYYRAADVCMVTSLHDGMNLVAKEYVAARDDERGALVLSQFTGAAHELHEALIVN
HHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHCCC
PYHVEQTAEALHRALTMPDYEQRERMRSMRMLVRDFNVYRWAGRMLLDAARVRQREKLAA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIGGES
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA