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The map label for this gene is pnp

Identifier: 308513305

GI number: 308513305

Start: 1746052

End: 1748145

Strand: Direct

Name: pnp

Synonym: NA

Alternate gene names: 308513305

Gene position: 1746052-1748145 (Clockwise)

Preceding gene: 39996692

Following gene: 39996694

Centisome position: 45.78

GC content: 58.26

Gene sequence:

>2094_bases
ATGACTGAACACAAGGTTAACGTAGAATTCGGTGGGCGGACCATAACGATCGCAACGGGCAAGTGGGCCAAGCAGGCTAG
TGGCGCCGTGGTGGTTAGTTGCGGGGACACCGTGGTTCTCGTGACTGCCGTAGCAACCAAATCGGCCCGGGAAGGACAGG
ACTTTTTCCCTCTGACGGTGAATTATCAGGAGAAAGCGTATGCCGGCGGCAAGATTCCCGGCGGCTTCTTCAAGCGCGAA
GGGCGTCCCTCCGACAACGAGACTCTCACGTCCCGCTTTATCGATCGTCCGATCCGTCCGCTTTTCCCTGAGAGTTTTCT
GAACGATACCCAGATTATGGCAACTGTCGTCTCGGCGGACCAGGATAATGATCCCGGCATCCTTGCCATGATCGGCGCAT
CTGCCGCCCTTGAGGTCTCCGATATTCCGTTCCTCGGCCCCATCGCCGGCGTCAAGGTTGGGCGCGTGGACGGCCAGTTT
GTATGCAATCCCACGGTAGAGCAACTGGAGAAAAGCGATCTTGAAATCGTTGTTGCGGCGAGCCGCGATGCGGTCATCAT
GGTAGAGGGGGGGGCTGCAGAGGCGTCCGAAAAGGATGTCCTGGAAGCTATTTTCTTCGGTCACGCCGCTGTTCAGCCGA
TTATCGAGGCCCAAACCGATCTCCGGAAGCTGGCTGGCGTTCCCAAGCGCGAGGTTGCGGCCACGTCTGTTGATGAGGCC
CTGAAGACTCGGGTGAAGGATCTTGCCTATGCCGGGATCAAGGAGGCCGTCCGGATCGTCGCCAAGCAGGAGCGTCACAA
CCGCATCGGCGAGATCACCGCGCAAACCCTTGAAACACTTCTGCCCGAGTATGAAGGACGTGAGTCGGAGATTAAGGGGT
TCCTCGGCGACTTCGAGTATGAACTCGTCCGTGAGCATATCATCAAGGACGGCTACCGCATTGACGGTCGGGACACCACA
ACTATCCGTCCTATCAGCATTGAGGTCAGCATGTTGCCGCGTGCCCACGGCTCCGCTCTCTTCACTCGTGGTGAAACCCA
GGCGCTCGTGGCATCGACTCTCGGGACTTCCATTGACGAGCAGCGGATTGATTCACTGTACGGCGAGACCCGCAAGCGCT
TCCTCCTTCACTACAACTTCCCGCCGTTCTCGGTCGGGGAAACGAGCTTCCGTCTCGCTCCGGGCCGGCGCGAAATCGGC
CACGGCATGCTGGCCGAAAGAGCTCTTGAGCGCGTCGTGCCCAAGCACGAGGATTTTCCGTACACCATCAGAATTGTCTC
CGATATCCTCGAGAGCAACGGTTCCTCCTCCATGGCAACCGTGTGCGGCGGCGCACTGGCGATGATGGATGCAGGCGTGC
CGATCAAGGCTCCGGTGGCCGGTATCGCCATGGGCCTCATCAAGGAAGGGGAGGGCATCGCCATCCTTTCCGACATTCTC
GGTGATGAGGATCATCTTGGCGATATGGATTTCAAGGTGGCCGGCACCGAAGCAGGGGTAACCGCCATCCAGATGGACAT
CAAGATCACCGGTGTCACCCGCGAGATCATGGAGAAAGCCTTGCTGCAGGCCCGTGACGGCAGACTCCATATCCTGGGCA
AGATGAATCAGGCCATTGCCGCTCCGCGGACTGATCTCTCACCCTATGCTCCCCGCATCACCACCATCTGGGTCAAGACC
GACAAGATCCGCGACGTCATCGGGTCAGGAGGAAAGAATATCCGCGGCATCACCGAGGCGACCGGCGTATCCATCGATAT
CGAGGACAGCGGCCGCATCAATATTGCCAGCACCAGCAAAGAAGCCTGCGACAAGGCTATCAAGATGATCCGCGACCTCA
CGGCCGAGGCCGAAGAGGGTAAGCTCTACATGGGCACCGTCAAAAAAGTCATGGATTTTGGCGCATTCGTGGAGATATTC
CCCGGCACCGACGGACTTGTCCATATATCCGAACTGGATACCGAGCGTGTGAAGAACGTGACCGACGTTCTCAATGAGGG
TGACAAGGTGCTGGTGAAGTGTATCGGCATAGACAAGCAGGGCAAGATAAAGCTGTCGCGGAAAGAGGCGCTCGGCGCCG
TACTGCCCGAATAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:
NA

Sequences:

>Translated_697_residues
MTEHKVNVEFGGRTITIATGKWAKQASGAVVVSCGDTVVLVTAVATKSAREGQDFFPLTVNYQEKAYAGGKIPGGFFKRE
GRPSDNETLTSRFIDRPIRPLFPESFLNDTQIMATVVSADQDNDPGILAMIGASAALEVSDIPFLGPIAGVKVGRVDGQF
VCNPTVEQLEKSDLEIVVAASRDAVIMVEGGAAEASEKDVLEAIFFGHAAVQPIIEAQTDLRKLAGVPKREVAATSVDEA
LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRDTT
TIRPISIEVSMLPRAHGSALFTRGETQALVASTLGTSIDEQRIDSLYGETRKRFLLHYNFPPFSVGETSFRLAPGRREIG
HGMLAERALERVVPKHEDFPYTIRIVSDILESNGSSSMATVCGGALAMMDAGVPIKAPVAGIAMGLIKEGEGIAILSDIL
GDEDHLGDMDFKVAGTEAGVTAIQMDIKITGVTREIMEKALLQARDGRLHILGKMNQAIAAPRTDLSPYAPRITTIWVKT
DKIRDVIGSGGKNIRGITEATGVSIDIEDSGRINIASTSKEACDKAIKMIRDLTAEAEEGKLYMGTVKKVMDFGAFVEIF
PGTDGLVHISELDTERVKNVTDVLNEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
>Mature_696_residues
TEHKVNVEFGGRTITIATGKWAKQASGAVVVSCGDTVVLVTAVATKSAREGQDFFPLTVNYQEKAYAGGKIPGGFFKREG
RPSDNETLTSRFIDRPIRPLFPESFLNDTQIMATVVSADQDNDPGILAMIGASAALEVSDIPFLGPIAGVKVGRVDGQFV
CNPTVEQLEKSDLEIVVAASRDAVIMVEGGAAEASEKDVLEAIFFGHAAVQPIIEAQTDLRKLAGVPKREVAATSVDEAL
KTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRDTTT
IRPISIEVSMLPRAHGSALFTRGETQALVASTLGTSIDEQRIDSLYGETRKRFLLHYNFPPFSVGETSFRLAPGRREIGH
GMLAERALERVVPKHEDFPYTIRIVSDILESNGSSSMATVCGGALAMMDAGVPIKAPVAGIAMGLIKEGEGIAILSDILG
DEDHLGDMDFKVAGTEAGVTAIQMDIKITGVTREIMEKALLQARDGRLHILGKMNQAIAAPRTDLSPYAPRITTIWVKTD
KIRDVIGSGGKNIRGITEATGVSIDIEDSGRINIASTSKEACDKAIKMIRDLTAEAEEGKLYMGTVKKVMDFGAFVEIFP
GTDGLVHISELDTERVKNVTDVLNEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=39.6332863187588, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=55.4904831625183, Blast_Score=748, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=722, Percent_Identity=36.1495844875346, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.5793871866295, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.5793871866295, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.5793871866295, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=37.6132930513595, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_GEOSL (Q74CS9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952644.3
- HSSP:   P05055
- ProteinModelPortal:   Q74CS9
- SMR:   Q74CS9
- GeneID:   2687125
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1593
- NMPDR:   fig|243231.1.peg.1582
- TIGR:   GSU1593
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   GSUL243231:GSU_1593-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75349; Mature: 75218

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHKVNVEFGGRTITIATGKWAKQASGAVVVSCGDTVVLVTAVATKSAREGQDFFPLTV
CCCEEEEEEECCEEEEEECCCCHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCCEEEEEE
NYQEKAYAGGKIPGGFFKREGRPSDNETLTSRFIDRPIRPLFPESFLNDTQIMATVVSAD
CCCHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHEEEEEECC
QDNDPGILAMIGASAALEVSDIPFLGPIAGVKVGRVDGQFVCNPTVEQLEKSDLEIVVAA
CCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCCEEEEEE
SRDAVIMVEGGAAEASEKDVLEAIFFGHAAVQPIIEAQTDLRKLAGVPKREVAATSVDEA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHH
ELVREHIIKDGYRIDGRDTTTIRPISIEVSMLPRAHGSALFTRGETQALVASTLGTSIDE
HHHHHHHHHCCCEECCCCCCEEEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCHH
QRIDSLYGETRKRFLLHYNFPPFSVGETSFRLAPGRREIGHGMLAERALERVVPKHEDFP
HHHHHHHHHHCEEEEEEECCCCCCCCCCCEEECCCHHHHHCHHHHHHHHHHHCCCCCCCC
YTIRIVSDILESNGSSSMATVCGGALAMMDAGVPIKAPVAGIAMGLIKEGEGIAILSDIL
EEHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHC
GDEDHLGDMDFKVAGTEAGVTAIQMDIKITGVTREIMEKALLQARDGRLHILGKMNQAIA
CCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHC
APRTDLSPYAPRITTIWVKTDKIRDVIGSGGKNIRGITEATGVSIDIEDSGRINIASTSK
CCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCEEEEECCCH
EACDKAIKMIRDLTAEAEEGKLYMGTVKKVMDFGAFVEIFPGTDGLVHISELDTERVKNV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEEHHCCHHHHHHH
TDVLNEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
HHHHCCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCC
>Mature Secondary Structure 
TEHKVNVEFGGRTITIATGKWAKQASGAVVVSCGDTVVLVTAVATKSAREGQDFFPLTV
CCEEEEEEECCEEEEEECCCCHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCCEEEEEE
NYQEKAYAGGKIPGGFFKREGRPSDNETLTSRFIDRPIRPLFPESFLNDTQIMATVVSAD
CCCHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHEEEEEECC
QDNDPGILAMIGASAALEVSDIPFLGPIAGVKVGRVDGQFVCNPTVEQLEKSDLEIVVAA
CCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCCEEEEEE
SRDAVIMVEGGAAEASEKDVLEAIFFGHAAVQPIIEAQTDLRKLAGVPKREVAATSVDEA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHH
ELVREHIIKDGYRIDGRDTTTIRPISIEVSMLPRAHGSALFTRGETQALVASTLGTSIDE
HHHHHHHHHCCCEECCCCCCEEEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCHH
QRIDSLYGETRKRFLLHYNFPPFSVGETSFRLAPGRREIGHGMLAERALERVVPKHEDFP
HHHHHHHHHHCEEEEEEECCCCCCCCCCCEEECCCHHHHHCHHHHHHHHHHHCCCCCCCC
YTIRIVSDILESNGSSSMATVCGGALAMMDAGVPIKAPVAGIAMGLIKEGEGIAILSDIL
EEHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHC
GDEDHLGDMDFKVAGTEAGVTAIQMDIKITGVTREIMEKALLQARDGRLHILGKMNQAIA
CCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHC
APRTDLSPYAPRITTIWVKTDKIRDVIGSGGKNIRGITEATGVSIDIEDSGRINIASTSK
CCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCEEEEECCCH
EACDKAIKMIRDLTAEAEEGKLYMGTVKKVMDFGAFVEIFPGTDGLVHISELDTERVKNV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEEHHCCHHHHHHH
TDVLNEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
HHHHCCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA