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The map label for this gene is oadA [H]

Identifier: 57235060

GI number: 57235060

Start: 116845

End: 118599

Strand: Direct

Name: oadA [H]

Synonym: DET0119

Alternate gene names: 57235060

Gene position: 116845-118599 (Clockwise)

Preceding gene: 57235065

Following gene: 57235052

Centisome position: 7.95

GC content: 52.25

Gene sequence:

>1755_bases
ATGGGTATAAAGATAACAGATACCACTCTGCGGGATGCGCACCAGTCACTGATAGCTACCCGGATGCGTACCCGTGACAT
GATTGATATTGCCGCCAAGCTGGATAAGGTCGGCTTTTATTCACTGGAGGTCTGGGGCGGCGCTACCTTTGACTCCTGCA
TCCGCTTCCTGAACGAAGACCCTTGGGAACGCCTGCGCCTTATACGCCAGAAAGCTCCCAATACCCCCCTCCAGATGCTT
CTCCGCGGGCAGAATCTGGTGGGATACTGTCACTATGCAGATGATGTAGTGCGTGAGTTTGTCCGTTTGTCCGTTAAAAA
CGGCATAGATATTTTCCGGGTTTTTGATGCCCTGAATGATACCCGCAATATGGAAGTTTCCGTTCAGGCGGCCAAAGAAC
TTAAGGCTCACGTTCAGGGTACTATCTGCTATACCACCAGCCCCATTCACACCGTAGAAAATCTGGCCGAGATGGCAGTG
GAACTGGAAAAGATGGGCTGTGATTCTATCTGTATTAAAGACATGGCCGGGCTTATTACCCCCACCGCTGCCGCCCAGCT
GGTAAAATCTATAAAAGCCAAGGTCAAACTGCCGGTAGACCTCCACTCCCACTGCACCAGCGGCATGGCACCTCTGGCTT
ATTATGCCGCGGCCGAGGCCGGGGTGGATATAATAGACACCGCCTTCTCCGCCTTTGCCTGGGGTACGTCCCAGCCCACT
ACCGAAAGTTTCGTGGCCGCTTTTCAGGGTACGAAACTGGATACCGGGCTTAATCTGGAACTGATGAGCGAAATAGGTGA
GGAGTTTAATAAAATAAGCTCTACTTACCGCTGCCTTTATACCTGCGAAGCCACCCAGCCGAGTATCGGGGTGCTTCTCC
ACCAGATACCCGGCGGTATGATTTCAAATCTGGTCAGCCAGCTTCGCCAGCAGAATGCCTTTGACAAACTGCCGCAGGTA
CTGGCCGAAGTACCCAAAGTAAGGGCGGATTTGGGCTACCCCCCGCTGGTTACCCCGTCAAGCCAGATTGTAGGCACGCA
GGCTATTTTAAATGTGCTGAGCGGTGCCCGCTACAAACAGGTTACCAAAGAAACTAAAAATTACCTGCTGGGGTATTACG
GCAAAATACCCGGTGCGGTAAATGAAGAAATACGCAAAGCTATTATCGGTGATGAAAAACCCATTAGTGTCAGGCCGGGG
GCTTTGCTTGAGCCGGAACTGCCCAAGATGAAGGCCGAAGGTGAAAAGCTGGGTATACTCAAAACCGAAGAAGACCTTTT
GACTTATGCCATGTACCCTGAGGTAGCGGCCAAGTTCCTCAAGGGTGAGTGCAAGGAAGAGTGCCTTTTGCCGTCCACCC
CTGAAGCAGCCAAAGTTGAAGCCAAAACCGAAGCTAAATCCGCTCCGGTCTTTAATGGTTCGGCTGAATACAGCGTTGAG
GTAGACGGAGAAGTCTTTACGGTCAAGGTTTCCGCTAAGGCCGGTGCGGTTGGCGAAGCCCCCAAAGCTGCCAAGCCTAC
GGCCTCACACCCCGGGGCAGTTGTCTCCCCCATGCAGGGTATGCTCCTTTCCCTGAAGGTGAAGGAAGGGGACAAGGTTA
GCGAAGGTGAAGTGGTGGCCACCATTGAGGCTATGAAGATGGAAAACGATGTTACCGCCGCTGTCAGCGGGGTAGTTTCA
GAGATATATGCTTATGAGGGCGAAGTGGTGGGCAGCAAAGACGTAATTATGGTGATTGAGCCAGATGCTAAATAA

Upstream 100 bases:

>100_bases
AGAATTAACTTCACTTCCAGCTTTTTTGCCTCAGTATTTTCAGATGTCCTTTCTGAGCTAATAGCAACCGGATTACTAAT
ATAGACGGTAGGTTAATAAT

Downstream 100 bases:

>100_bases
AATACTGGTTGCCAACAGGGGTGAGATAGCCATACGTATTATGCGCGCCTGCCGTGAGCTGGGCATAAAGACTGTTGCCG
TTTATTCCGATGCTGACAAA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MGIKITDTTLRDAHQSLIATRMRTRDMIDIAAKLDKVGFYSLEVWGGATFDSCIRFLNEDPWERLRLIRQKAPNTPLQML
LRGQNLVGYCHYADDVVREFVRLSVKNGIDIFRVFDALNDTRNMEVSVQAAKELKAHVQGTICYTTSPIHTVENLAEMAV
ELEKMGCDSICIKDMAGLITPTAAAQLVKSIKAKVKLPVDLHSHCTSGMAPLAYYAAAEAGVDIIDTAFSAFAWGTSQPT
TESFVAAFQGTKLDTGLNLELMSEIGEEFNKISSTYRCLYTCEATQPSIGVLLHQIPGGMISNLVSQLRQQNAFDKLPQV
LAEVPKVRADLGYPPLVTPSSQIVGTQAILNVLSGARYKQVTKETKNYLLGYYGKIPGAVNEEIRKAIIGDEKPISVRPG
ALLEPELPKMKAEGEKLGILKTEEDLLTYAMYPEVAAKFLKGECKEECLLPSTPEAAKVEAKTEAKSAPVFNGSAEYSVE
VDGEVFTVKVSAKAGAVGEAPKAAKPTASHPGAVVSPMQGMLLSLKVKEGDKVSEGEVVATIEAMKMENDVTAAVSGVVS
EIYAYEGEVVGSKDVIMVIEPDAK

Sequences:

>Translated_584_residues
MGIKITDTTLRDAHQSLIATRMRTRDMIDIAAKLDKVGFYSLEVWGGATFDSCIRFLNEDPWERLRLIRQKAPNTPLQML
LRGQNLVGYCHYADDVVREFVRLSVKNGIDIFRVFDALNDTRNMEVSVQAAKELKAHVQGTICYTTSPIHTVENLAEMAV
ELEKMGCDSICIKDMAGLITPTAAAQLVKSIKAKVKLPVDLHSHCTSGMAPLAYYAAAEAGVDIIDTAFSAFAWGTSQPT
TESFVAAFQGTKLDTGLNLELMSEIGEEFNKISSTYRCLYTCEATQPSIGVLLHQIPGGMISNLVSQLRQQNAFDKLPQV
LAEVPKVRADLGYPPLVTPSSQIVGTQAILNVLSGARYKQVTKETKNYLLGYYGKIPGAVNEEIRKAIIGDEKPISVRPG
ALLEPELPKMKAEGEKLGILKTEEDLLTYAMYPEVAAKFLKGECKEECLLPSTPEAAKVEAKTEAKSAPVFNGSAEYSVE
VDGEVFTVKVSAKAGAVGEAPKAAKPTASHPGAVVSPMQGMLLSLKVKEGDKVSEGEVVATIEAMKMENDVTAAVSGVVS
EIYAYEGEVVGSKDVIMVIEPDAK
>Mature_583_residues
GIKITDTTLRDAHQSLIATRMRTRDMIDIAAKLDKVGFYSLEVWGGATFDSCIRFLNEDPWERLRLIRQKAPNTPLQMLL
RGQNLVGYCHYADDVVREFVRLSVKNGIDIFRVFDALNDTRNMEVSVQAAKELKAHVQGTICYTTSPIHTVENLAEMAVE
LEKMGCDSICIKDMAGLITPTAAAQLVKSIKAKVKLPVDLHSHCTSGMAPLAYYAAAEAGVDIIDTAFSAFAWGTSQPTT
ESFVAAFQGTKLDTGLNLELMSEIGEEFNKISSTYRCLYTCEATQPSIGVLLHQIPGGMISNLVSQLRQQNAFDKLPQVL
AEVPKVRADLGYPPLVTPSSQIVGTQAILNVLSGARYKQVTKETKNYLLGYYGKIPGAVNEEIRKAIIGDEKPISVRPGA
LLEPELPKMKAEGEKLGILKTEEDLLTYAMYPEVAAKFLKGECKEECLLPSTPEAAKVEAKTEAKSAPVFNGSAEYSVEV
DGEVFTVKVSAKAGAVGEAPKAAKPTASHPGAVVSPMQGMLLSLKVKEGDKVSEGEVVATIEAMKMENDVTAAVSGVVSE
IYAYEGEVVGSKDVIMVIEPDAK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=621, Percent_Identity=34.4605475040258, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI106049295, Length=621, Percent_Identity=34.4605475040258, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI106049292, Length=621, Percent_Identity=34.4605475040258, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17562816, Length=625, Percent_Identity=33.92, Blast_Score=312, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6319695, Length=620, Percent_Identity=33.0645161290323, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6321376, Length=617, Percent_Identity=32.4149108589951, Blast_Score=319, Evalue=9e-88,
Organism=Drosophila melanogaster, GI24652212, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652210, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652214, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI19921944, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652216, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI281363050, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652224, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652222, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652220, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652218, Length=620, Percent_Identity=36.7741935483871, Blast_Score=327, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63280; Mature: 63148

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKITDTTLRDAHQSLIATRMRTRDMIDIAAKLDKVGFYSLEVWGGATFDSCIRFLNED
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCC
PWERLRLIRQKAPNTPLQMLLRGQNLVGYCHYADDVVREFVRLSVKNGIDIFRVFDALND
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
TRNMEVSVQAAKELKAHVQGTICYTTSPIHTVENLAEMAVELEKMGCDSICIKDMAGLIT
CCCCEEEHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PTAAAQLVKSIKAKVKLPVDLHSHCTSGMAPLAYYAAAEAGVDIIDTAFSAFAWGTSQPT
HHHHHHHHHHHHHHEECCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TESFVAAFQGTKLDTGLNLELMSEIGEEFNKISSTYRCLYTCEATQPSIGVLLHQIPGGM
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCHH
ISNLVSQLRQQNAFDKLPQVLAEVPKVRADLGYPPLVTPSSQIVGTQAILNVLSGARYKQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
VTKETKNYLLGYYGKIPGAVNEEIRKAIIGDEKPISVRPGALLEPELPKMKAEGEKLGIL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCE
KTEEDLLTYAMYPEVAAKFLKGECKEECLLPSTPEAAKVEAKTEAKSAPVFNGSAEYSVE
ECHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHEEEHHHHHCCCCCCCCCCCEEEE
VDGEVFTVKVSAKAGAVGEAPKAAKPTASHPGAVVSPMQGMLLSLKVKEGDKVSEGEVVA
ECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCEEE
TIEAMKMENDVTAAVSGVVSEIYAYEGEVVGSKDVIMVIEPDAK
EEEHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCC
>Mature Secondary Structure 
GIKITDTTLRDAHQSLIATRMRTRDMIDIAAKLDKVGFYSLEVWGGATFDSCIRFLNED
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCC
PWERLRLIRQKAPNTPLQMLLRGQNLVGYCHYADDVVREFVRLSVKNGIDIFRVFDALND
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
TRNMEVSVQAAKELKAHVQGTICYTTSPIHTVENLAEMAVELEKMGCDSICIKDMAGLIT
CCCCEEEHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PTAAAQLVKSIKAKVKLPVDLHSHCTSGMAPLAYYAAAEAGVDIIDTAFSAFAWGTSQPT
HHHHHHHHHHHHHHEECCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TESFVAAFQGTKLDTGLNLELMSEIGEEFNKISSTYRCLYTCEATQPSIGVLLHQIPGGM
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCHH
ISNLVSQLRQQNAFDKLPQVLAEVPKVRADLGYPPLVTPSSQIVGTQAILNVLSGARYKQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
VTKETKNYLLGYYGKIPGAVNEEIRKAIIGDEKPISVRPGALLEPELPKMKAEGEKLGIL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCE
KTEEDLLTYAMYPEVAAKFLKGECKEECLLPSTPEAAKVEAKTEAKSAPVFNGSAEYSVE
ECHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHEEEHHHHHCCCCCCCCCCCEEEE
VDGEVFTVKVSAKAGAVGEAPKAAKPTASHPGAVVSPMQGMLLSLKVKEGDKVSEGEVVA
ECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCEEE
TIEAMKMENDVTAAVSGVVSEIYAYEGEVVGSKDVIMVIEPDAK
EEEHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]