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The map label for this gene is ntcA [H]

Identifier: 57234871

GI number: 57234871

Start: 286986

End: 287729

Strand: Direct

Name: ntcA [H]

Synonym: DET0299

Alternate gene names: 57234871

Gene position: 286986-287729 (Clockwise)

Preceding gene: 57234883

Following gene: 57234868

Centisome position: 19.53

GC content: 45.56

Gene sequence:

>744_bases
GTGAAATATTTGAACTGCATGATGGATTTGTGGCTGTTTGACTCCCTTAACCCGCAGGAGAAGACCGAGATAAGCCGCCT
TTTCAGGCGTCCGGAATATTTAAAGAATGAGTATCTTTTCAGCGAAGGCGAACCGGCCAGTGCCGTTTTTGTGGTGGCTA
AAGGCCGGGTGAAGCTCTTTAAAACGGCTGAAAATGGGCGGGAGATTATTCTGGCTTATTTGACTCAGAACCAGCTCTTC
GGGGAAGAGATTTTGTTTAATGATGCTATCCGGACTATTACCGCCGTGGCCATGGAAGATACCCGGCTGTGTGCCTGTTA
TAAAAGTGACTTTGAAAATTTGCTTTCGCAAAATTCGCAGATAGCGGTTAAAGTTATCCGCACCCTCAGTGAAAAAATAA
ATAACATTACCGAAACTCTGGCGGATATGGCTATTTACGATATCCAAAACCGGCTGGCGCGCACTCTGGCCAGGCTGGCT
AAAGAACACGGCGAAGATGTAGGTGATGGCAGACGCCTAAATTTCCGTTTAACCCACGATGACCTTGGTTCTTTAGTGGG
GGCATCCCGGGTGATGGTGACTAATGTTATAAAATCCCTGAAAAAATCCGGCATAATCAAAGATGATATTGACCATAAGC
TGGTGGTCAGCCAGTGGTTTCTGAATGATTTTTCTGATGAAATGCCCAAATTTATCAGAAAAGATTCAAGCGCCTGCAAG
TGTTTCCCGAAACCCGGCGAATAA

Upstream 100 bases:

>100_bases
TTTGCATACTCAGGGCGGCTTAGTCTGTAAAATTTATTACATATTCAGCGTCCGGTTTTTTGTATAATCTAGGTATAAAC
CGAAAACGGGGAGGACTCAG

Downstream 100 bases:

>100_bases
GTATCTTAGCAGTAAATGTTTAATGCATCTTTCAGGGGGAAGAGGCGCTTCAGGTCTTTTTGTTATAACTATATAGCGGT
TTATTCGGTAATCTGATAGC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKYLNCMMDLWLFDSLNPQEKTEISRLFRRPEYLKNEYLFSEGEPASAVFVVAKGRVKLFKTAENGREIILAYLTQNQLF
GEEILFNDAIRTITAVAMEDTRLCACYKSDFENLLSQNSQIAVKVIRTLSEKINNITETLADMAIYDIQNRLARTLARLA
KEHGEDVGDGRRLNFRLTHDDLGSLVGASRVMVTNVIKSLKKSGIIKDDIDHKLVVSQWFLNDFSDEMPKFIRKDSSACK
CFPKPGE

Sequences:

>Translated_247_residues
MKYLNCMMDLWLFDSLNPQEKTEISRLFRRPEYLKNEYLFSEGEPASAVFVVAKGRVKLFKTAENGREIILAYLTQNQLF
GEEILFNDAIRTITAVAMEDTRLCACYKSDFENLLSQNSQIAVKVIRTLSEKINNITETLADMAIYDIQNRLARTLARLA
KEHGEDVGDGRRLNFRLTHDDLGSLVGASRVMVTNVIKSLKKSGIIKDDIDHKLVVSQWFLNDFSDEMPKFIRKDSSACK
CFPKPGE
>Mature_247_residues
MKYLNCMMDLWLFDSLNPQEKTEISRLFRRPEYLKNEYLFSEGEPASAVFVVAKGRVKLFKTAENGREIILAYLTQNQLF
GEEILFNDAIRTITAVAMEDTRLCACYKSDFENLLSQNSQIAVKVIRTLSEKINNITETLADMAIYDIQNRLARTLARLA
KEHGEDVGDGRRLNFRLTHDDLGSLVGASRVMVTNVIKSLKKSGIIKDDIDHKLVVSQWFLNDFSDEMPKFIRKDSSACK
CFPKPGE

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=182, Percent_Identity=28.021978021978, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28230; Mature: 28230

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLNCMMDLWLFDSLNPQEKTEISRLFRRPEYLKNEYLFSEGEPASAVFVVAKGRVKLF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEEEECCCEEEE
KTAENGREIILAYLTQNQLFGEEILFNDAIRTITAVAMEDTRLCACYKSDFENLLSQNSQ
EECCCCCEEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
IAVKVIRTLSEKINNITETLADMAIYDIQNRLARTLARLAKEHGEDVGDGRRLNFRLTHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHH
DLGSLVGASRVMVTNVIKSLKKSGIIKDDIDHKLVVSQWFLNDFSDEMPKFIRKDSSACK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CFPKPGE
CCCCCCC
>Mature Secondary Structure
MKYLNCMMDLWLFDSLNPQEKTEISRLFRRPEYLKNEYLFSEGEPASAVFVVAKGRVKLF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEEEECCCEEEE
KTAENGREIILAYLTQNQLFGEEILFNDAIRTITAVAMEDTRLCACYKSDFENLLSQNSQ
EECCCCCEEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
IAVKVIRTLSEKINNITETLADMAIYDIQNRLARTLARLAKEHGEDVGDGRRLNFRLTHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHH
DLGSLVGASRVMVTNVIKSLKKSGIIKDDIDHKLVVSQWFLNDFSDEMPKFIRKDSSACK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CFPKPGE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]