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The map label for this gene is cprA [H]

Identifier: 57234851

GI number: 57234851

Start: 298706

End: 300253

Strand: Direct

Name: cprA [H]

Synonym: DET0311

Alternate gene names: 57234851

Gene position: 298706-300253 (Clockwise)

Preceding gene: 57234852

Following gene: 57234850

Centisome position: 20.32

GC content: 45.93

Gene sequence:

>1548_bases
ATGGATAAATTTCATTCAACACTCAGCCGGCGTGATTTTATGAAGGCGGTTGGATTGGCCGGTGCTGGGGTCGGTGCGGT
AACTGCCGCATCACCTGTGTTTCATGATCTGGACGAAGTTTCTCAGATTGGTGGAGTAACTGAAAAACGCCCCTGGTATG
TGCGTGAGTTGGAATATGCCAAACCCACAGTGGAAATAGATTGGAATGTCATTCAGCGCCAAACTCATTATAACAACTGG
GAAGATCATCTAGACCAGGCTGAAAAAGATAAACGCGCGCACATGTATTATGACAACACAAAGCAGATGGTTTTGGAAAA
TAATCCAGGTAATACTATGTTTGATGTTGCTATACGTCACCCGGCTTGGACAGCTGTCCGGCGTAACATGGATTATTTTT
TTGGGGTGGAGGGTATTCAGCAGGATACTCCGCCTGGTTTTGAAATGGGTTTTGATCCTGAGTCAGGGCATTGCGTTATC
AGCCCTGTATGGGCTACCGATCTTTTTGGGATAATCACACCTGATAATATGGGAGTGCCCCGGTGGGAAGGCACACCCGA
ACAGAATGCAGCTTTAATCCGCATGGCTGCTAGATGGTGCGGCGGGGCTGAAGTGGGCTACTTGAAGGCAGATGAATATA
CCAAGAAACTTGTACATAAGACATGCGGCGTTTTACCGATTCTGGCAGATAAGAATGGCAAAGAAGTAGTTTGGGAAGAT
GTTGATCAGCCATACGAAACAGATAAAAAAATGGTTATCCCAGAAAAGTGCAACAACATAATAGTAGTTGTAATACGCGA
GGCTAGAAATCCGGCTTTGATGGCGCCGAGCTATAGGGCGGATGCTACCACTGCCAAATCTTATGCTCGTTCAATTGCAT
TTGATATCCATTTCAGAGGATTTATTCATGCCATTGGCTATACTACTGCAGGTTCAGGCTGGGGCCCCTGGAATAACGTG
CCGTTCGGAGTACTATCTGGGATAGGTGAGTTGGGGCGTATGAGAGGGCAAATAACCCCATCTTGTGGTCCATTAATTCG
TAAGGTGGAGGTGTTTTTCACTGATCTCCCGTTACCCACTACTAATCCCATAGACTTCGGGGCTAATCGTTTCTGCAGGG
ATTGCGGATTATGTGCCAAAGCTTGCCCTGCTTCAGCTATACCGACTTTCCGTGAACCGACCTATGAGATTACTCCCGCT
GATGATGCTAATTCCAATCCGACCAAGCTTATACCGGAATACTTTAATCTATCCGGTAAAAAGGTCTGGCCTAACAACGA
CTTTGCCTGCCACAATTTCTGGGTGACTTCTGGTAAACATGGGTGTGCGGCTTGCGTAGCTTCTTGCGTATTTAGCAAGG
ATATCAAGTCCAGTATCCACGAAGTAGTTAAAGGCGTAGTTTCTCAGACAGGTATCTTCAACGGCTTTTTTGCCAATATG
GACCATGCCTTTGGTTACGGCATAGTCAAAGACCAGAATATGTGGGACAACTTCTGGTTTGAACCTGATAAATACTGGCC
GCTTGAAGGTATTGAAACCAATTTATAA

Upstream 100 bases:

>100_bases
TCAATAAAACTATAGTAGAGGAGGTGTAACTGGGACTGACTTTCGGGAGAGACCGGATAACCCCATTTGGATTTTAACTA
AGGTAAAGGAGGAGACTATT

Downstream 100 bases:

>100_bases
AGACTGGCAATAGCCTTATTTTCTAGAAAGGAAGTTAGAAATATGTGGTCTCTGTATACTCTCATATGGATGCTCATAGG
TATCGCTTTAGGCGTAGGTT

Product: reductive dehalogenase, putative

Products: NA

Alternate protein names: C1-OHPA dehalogenase; O-chlorophenol reductive dehalogenase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MDKFHSTLSRRDFMKAVGLAGAGVGAVTAASPVFHDLDEVSQIGGVTEKRPWYVRELEYAKPTVEIDWNVIQRQTHYNNW
EDHLDQAEKDKRAHMYYDNTKQMVLENNPGNTMFDVAIRHPAWTAVRRNMDYFFGVEGIQQDTPPGFEMGFDPESGHCVI
SPVWATDLFGIITPDNMGVPRWEGTPEQNAALIRMAARWCGGAEVGYLKADEYTKKLVHKTCGVLPILADKNGKEVVWED
VDQPYETDKKMVIPEKCNNIIVVVIREARNPALMAPSYRADATTAKSYARSIAFDIHFRGFIHAIGYTTAGSGWGPWNNV
PFGVLSGIGELGRMRGQITPSCGPLIRKVEVFFTDLPLPTTNPIDFGANRFCRDCGLCAKACPASAIPTFREPTYEITPA
DDANSNPTKLIPEYFNLSGKKVWPNNDFACHNFWVTSGKHGCAACVASCVFSKDIKSSIHEVVKGVVSQTGIFNGFFANM
DHAFGYGIVKDQNMWDNFWFEPDKYWPLEGIETNL

Sequences:

>Translated_515_residues
MDKFHSTLSRRDFMKAVGLAGAGVGAVTAASPVFHDLDEVSQIGGVTEKRPWYVRELEYAKPTVEIDWNVIQRQTHYNNW
EDHLDQAEKDKRAHMYYDNTKQMVLENNPGNTMFDVAIRHPAWTAVRRNMDYFFGVEGIQQDTPPGFEMGFDPESGHCVI
SPVWATDLFGIITPDNMGVPRWEGTPEQNAALIRMAARWCGGAEVGYLKADEYTKKLVHKTCGVLPILADKNGKEVVWED
VDQPYETDKKMVIPEKCNNIIVVVIREARNPALMAPSYRADATTAKSYARSIAFDIHFRGFIHAIGYTTAGSGWGPWNNV
PFGVLSGIGELGRMRGQITPSCGPLIRKVEVFFTDLPLPTTNPIDFGANRFCRDCGLCAKACPASAIPTFREPTYEITPA
DDANSNPTKLIPEYFNLSGKKVWPNNDFACHNFWVTSGKHGCAACVASCVFSKDIKSSIHEVVKGVVSQTGIFNGFFANM
DHAFGYGIVKDQNMWDNFWFEPDKYWPLEGIETNL
>Mature_515_residues
MDKFHSTLSRRDFMKAVGLAGAGVGAVTAASPVFHDLDEVSQIGGVTEKRPWYVRELEYAKPTVEIDWNVIQRQTHYNNW
EDHLDQAEKDKRAHMYYDNTKQMVLENNPGNTMFDVAIRHPAWTAVRRNMDYFFGVEGIQQDTPPGFEMGFDPESGHCVI
SPVWATDLFGIITPDNMGVPRWEGTPEQNAALIRMAARWCGGAEVGYLKADEYTKKLVHKTCGVLPILADKNGKEVVWED
VDQPYETDKKMVIPEKCNNIIVVVIREARNPALMAPSYRADATTAKSYARSIAFDIHFRGFIHAIGYTTAGSGWGPWNNV
PFGVLSGIGELGRMRGQITPSCGPLIRKVEVFFTDLPLPTTNPIDFGANRFCRDCGLCAKACPASAIPTFREPTYEITPA
DDANSNPTKLIPEYFNLSGKKVWPNNDFACHNFWVTSGKHGCAACVASCVFSKDIKSSIHEVVKGVVSQTGIFNGFFANM
DHAFGYGIVKDQNMWDNFWFEPDKYWPLEGIETNL

Specific function: Catalyzes the reductive dechlorination of C1-OHPA to 4- hydroxyphenylacetate with reduced methyl viologen as the electron donor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012832
- InterPro:   IPR006311
- InterPro:   IPR019546 [H]

Pfam domain/function: PF10518 TAT_signal [H]

EC number: NA

Molecular weight: Translated: 57594; Mature: 57594

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFHSTLSRRDFMKAVGLAGAGVGAVTAASPVFHDLDEVSQIGGVTEKRPWYVRELEYA
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHCC
KPTVEIDWNVIQRQTHYNNWEDHLDQAEKDKRAHMYYDNTKQMVLENNPGNTMFDVAIRH
CCEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCEEEEEEECC
PAWTAVRRNMDYFFGVEGIQQDTPPGFEMGFDPESGHCVISPVWATDLFGIITPDNMGVP
CHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCEEEEECCCCCCCC
RWEGTPEQNAALIRMAARWCGGAEVGYLKADEYTKKLVHKTCGVLPILADKNGKEVVWED
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCEEHHHC
VDQPYETDKKMVIPEKCNNIIVVVIREARNPALMAPSYRADATTAKSYARSIAFDIHFRG
CCCCCCCCCCEECHHHCCCEEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHEEEEEHHH
FIHAIGYTTAGSGWGPWNNVPFGVLSGIGELGRMRGQITPSCGPLIRKVEVFFTDLPLPT
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCC
TNPIDFGANRFCRDCGLCAKACPASAIPTFREPTYEITPADDANSNPTKLIPEYFNLSGK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEECHHHHCCCCC
KVWPNNDFACHNFWVTSGKHGCAACVASCVFSKDIKSSIHEVVKGVVSQTGIFNGFFANM
EECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
DHAFGYGIVKDQNMWDNFWFEPDKYWPLEGIETNL
CHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDKFHSTLSRRDFMKAVGLAGAGVGAVTAASPVFHDLDEVSQIGGVTEKRPWYVRELEYA
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHCC
KPTVEIDWNVIQRQTHYNNWEDHLDQAEKDKRAHMYYDNTKQMVLENNPGNTMFDVAIRH
CCEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCEEEEEEECC
PAWTAVRRNMDYFFGVEGIQQDTPPGFEMGFDPESGHCVISPVWATDLFGIITPDNMGVP
CHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCEEEEECCCCCCCC
RWEGTPEQNAALIRMAARWCGGAEVGYLKADEYTKKLVHKTCGVLPILADKNGKEVVWED
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCEEHHHC
VDQPYETDKKMVIPEKCNNIIVVVIREARNPALMAPSYRADATTAKSYARSIAFDIHFRG
CCCCCCCCCCEECHHHCCCEEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHEEEEEHHH
FIHAIGYTTAGSGWGPWNNVPFGVLSGIGELGRMRGQITPSCGPLIRKVEVFFTDLPLPT
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCC
TNPIDFGANRFCRDCGLCAKACPASAIPTFREPTYEITPADDANSNPTKLIPEYFNLSGK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEECHHHHCCCCC
KVWPNNDFACHNFWVTSGKHGCAACVASCVFSKDIKSSIHEVVKGVVSQTGIFNGFFANM
EECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
DHAFGYGIVKDQNMWDNFWFEPDKYWPLEGIETNL
CHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9781670 [H]