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The map label for this gene is dagK [H]

Identifier: 57234806

GI number: 57234806

Start: 390526

End: 391431

Strand: Direct

Name: dagK [H]

Synonym: DET0414

Alternate gene names: 57234806

Gene position: 390526-391431 (Clockwise)

Preceding gene: 57234807

Following gene: 57234804

Centisome position: 26.57

GC content: 50.33

Gene sequence:

>906_bases
TTGGTAGCAAAAGTAATAGTTAACCCGGTGGCGGGGGCAAAGTCCACTGAACAAAAATGGCCGCATATCAGCAGGCTTAT
GTGTGATATGGGCTTTTCTTATGATTTTCAGTACACCGAAAGCCAGGGGCACGCTATTGAGATTGCCCGTGATGCCGCCT
TAAACGGTTATCCTTATCTGGTAGCGGTGGGCGGTGACGGCACTATTAACGAAGTAGTTAACGGTATTCTGACCGCCAGC
CATGACCAAAAAACCCTGATGGGGGTGGTGGATACCGGCACCGGCAATGATTTTGTGCGTTCGCTGGGCCTTGACGGTAA
TTATCTCCATTCCTGCCAGCATTTGCTCAGCCCCAAGCACACTTTGGTGGATGCCGGTCTGGTAACGTTTCAAAAAGACG
GCCGTCAGGTCAGCCGCTTCTTTGTAAACGGAGCAGGGGTGGGCTTTGATGCCGAGGTGGCAGCTACGACCGAACATATG
CCTAAAGCTCTGGGCGGCACTATCCCCTTTGTCATGGCTCTGGCAAAAACCCTGCTTGGATACCGTAATAAAACTGTAGA
TATAGGGCTGGATGAAGATAACTATACCCGGCGTGTTTTAAGCGTGATTGTAGCCAACGGCAGTTATTTCGGCGGCGGCA
TGAAGGTAGCCCCGTCCGCCCTTATTACTGACAGCCGGTTTGATGTTATCACTCTGGGTGACGTAAACAAGCTGGAAATC
CTGCAAACCTTCCCCAAAATCTACAAGGGTACTCATATTACCCATCCCAAGGTAAAAACCGAACACGCCCGTTTTGTGAG
TATTAGTTCTGAAGAGGGGCTGTATCTTCAGGCAGATGGGGAGCTTTTAGGTAAAACCCCGGCAACCTTTGAGGTCTTGC
CTCAGGCACTTACCATGGTTTTCTAG

Upstream 100 bases:

>100_bases
ATATCTGGACTAAGAAGTATCAAAAATGGTCAAAAATTCGGAATAGCCAAAACGGTATTGCAGTGTTATAATTAGTGCAT
ATCAATGTGAGTTAAAGTAT

Downstream 100 bases:

>100_bases
ATTCGCCCCTGCTGTTTTTCTCTTTCACTAAAAACCGGGACTATCTGTCTTGTTCTCTGTTTATGCTAGCTTTTATTTTC
GTTCTGATAAATGCCGCAGT

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVAKVIVNPVAGAKSTEQKWPHISRLMCDMGFSYDFQYTESQGHAIEIARDAALNGYPYLVAVGGDGTINEVVNGILTAS
HDQKTLMGVVDTGTGNDFVRSLGLDGNYLHSCQHLLSPKHTLVDAGLVTFQKDGRQVSRFFVNGAGVGFDAEVAATTEHM
PKALGGTIPFVMALAKTLLGYRNKTVDIGLDEDNYTRRVLSVIVANGSYFGGGMKVAPSALITDSRFDVITLGDVNKLEI
LQTFPKIYKGTHITHPKVKTEHARFVSISSEEGLYLQADGELLGKTPATFEVLPQALTMVF

Sequences:

>Translated_301_residues
MVAKVIVNPVAGAKSTEQKWPHISRLMCDMGFSYDFQYTESQGHAIEIARDAALNGYPYLVAVGGDGTINEVVNGILTAS
HDQKTLMGVVDTGTGNDFVRSLGLDGNYLHSCQHLLSPKHTLVDAGLVTFQKDGRQVSRFFVNGAGVGFDAEVAATTEHM
PKALGGTIPFVMALAKTLLGYRNKTVDIGLDEDNYTRRVLSVIVANGSYFGGGMKVAPSALITDSRFDVITLGDVNKLEI
LQTFPKIYKGTHITHPKVKTEHARFVSISSEEGLYLQADGELLGKTPATFEVLPQALTMVF
>Mature_301_residues
MVAKVIVNPVAGAKSTEQKWPHISRLMCDMGFSYDFQYTESQGHAIEIARDAALNGYPYLVAVGGDGTINEVVNGILTAS
HDQKTLMGVVDTGTGNDFVRSLGLDGNYLHSCQHLLSPKHTLVDAGLVTFQKDGRQVSRFFVNGAGVGFDAEVAATTEHM
PKALGGTIPFVMALAKTLLGYRNKTVDIGLDEDNYTRRVLSVIVANGSYFGGGMKVAPSALITDSRFDVITLGDVNKLEI
LQTFPKIYKGTHITHPKVKTEHARFVSISSEEGLYLQADGELLGKTPATFEVLPQALTMVF

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=273, Percent_Identity=24.9084249084249, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32527; Mature: 32527

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKVIVNPVAGAKSTEQKWPHISRLMCDMGFSYDFQYTESQGHAIEIARDAALNGYPYL
CCCEEEECCCCCCCCCHHHCHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCEE
VAVGGDGTINEVVNGILTASHDQKTLMGVVDTGTGNDFVRSLGLDGNYLHSCQHLLSPKH
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCCCH
TLVDAGLVTFQKDGRQVSRFFVNGAGVGFDAEVAATTEHMPKALGGTIPFVMALAKTLLG
HHHHCCCEEECCCCHHEEHHEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
YRNKTVDIGLDEDNYTRRVLSVIVANGSYFGGGMKVAPSALITDSRFDVITLGDVNKLEI
CCCCEEEEECCCCHHHHHHHHHHHCCCCEECCCCEECCCEEEECCCCCEEEECCCCHHHH
LQTFPKIYKGTHITHPKVKTEHARFVSISSEEGLYLQADGELLGKTPATFEVLPQALTMV
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEECCCCCHHHHHHHHHHHC
F
C
>Mature Secondary Structure
MVAKVIVNPVAGAKSTEQKWPHISRLMCDMGFSYDFQYTESQGHAIEIARDAALNGYPYL
CCCEEEECCCCCCCCCHHHCHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCEE
VAVGGDGTINEVVNGILTASHDQKTLMGVVDTGTGNDFVRSLGLDGNYLHSCQHLLSPKH
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCCCH
TLVDAGLVTFQKDGRQVSRFFVNGAGVGFDAEVAATTEHMPKALGGTIPFVMALAKTLLG
HHHHCCCEEECCCCHHEEHHEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
YRNKTVDIGLDEDNYTRRVLSVIVANGSYFGGGMKVAPSALITDSRFDVITLGDVNKLEI
CCCCEEEEECCCCHHHHHHHHHHHCCCCEECCCCEECCCEEEECCCCCEEEECCCCHHHH
LQTFPKIYKGTHITHPKVKTEHARFVSISSEEGLYLQADGELLGKTPATFEVLPQALTMV
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEECCCCCHHHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA