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The map label for this gene is aspS [H]

Identifier: 57234556

GI number: 57234556

Start: 623028

End: 623918

Strand: Direct

Name: aspS [H]

Synonym: DET0674

Alternate gene names: 57234556

Gene position: 623028-623918 (Clockwise)

Preceding gene: 57234557

Following gene: 57234555

Centisome position: 42.39

GC content: 46.24

Gene sequence:

>891_bases
ATGCTTAAAACTCATAGCTGTGCCTTAACTCAGGAAAATATTGGCACTGAAGTTACTCTGGCAGGTTGGGTACACCGCCG
CCGAGACCATGGCGGGGTTATATTTATTGACCTTCGTGACCGGGAAGGCATTGTTCAGGTGGTATTTAACCCCGAACAGT
CCGCTGCCTGTCTGGATATCGGTAAAGAACTGCGGAGTGAATATGTCCTGCAGGTAAAAGGCATTAAATCCAGCGGAAAA
ATGTTTTGCGGTGAACTGCTGCCGCTCATTTTGAAGTGTATAAGGGATAATGATATTGACCTGCTGTGTGTGGCTTCTCA
TGGATATACCGGTATTAAGAAAATGGTACTGGGCAGCGTTTCCGAAAAGCTTATTAAATCTACCAATCTGCCGGTTTTGC
TGGTAAAAGATGGGTACGACGCAGACAAGGCAGCTTTCGGGAATATTTTATTGCCACTGGATGGCTCTCTTTTTTCAGAG
GCTTGTCTGAAGCAGGTATCAGTTCTGGCCCGTGATTTTAAAAGCAAACTTACCCTGCTGGTGGTTATTCAGCCCCCCTG
CATACCCTCAGATCGTTCCCCCAGTATTCAACCCAGCTGGGATGAATACTATCAGGCGCTTCTGGAGGAGCTTAAACAAC
AGGCGCAGGCCTATCTGGAGCGGCTTACCGGCGAATTGCGGGAAAATGGAATTGAAACTGAATTCCGTATGGAAGTTTCA
GATAATATTCCTGAAACTATTTTGAGTACCGCCGAAGATATAAATGCCTCTCTTATTTCATTTACCACCCATAACCGCTC
CGGAATAGAGAAATGGTATTATGGAAGTGTGGCAGCCGGTTTGATTGAAAATTCCCCGCGCCCCATGCTGTTATGGCGGA
ACAGCATTTAA

Upstream 100 bases:

>100_bases
CTGGGCGAATACGAAAAAAAATAGCTTTCAGCGTTTGAACAAACACAATGTATAGGATATAATCTTTTCTAATTGTCTTG
ACTAAATTTAGAAGGTGCGC

Downstream 100 bases:

>100_bases
ACTTTGCTTGACACTTGCCTTTACTCCTTCCTACAATTAGACAAGAAAAAGGCATGAAAAAGATTTATGACTTAAGCCCC
GAAATACGCCCTGAAATGAT

Product: universal stress protein

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGIKSSGK
MFCGELLPLILKCIRDNDIDLLCVASHGYTGIKKMVLGSVSEKLIKSTNLPVLLVKDGYDADKAAFGNILLPLDGSLFSE
ACLKQVSVLARDFKSKLTLLVVIQPPCIPSDRSPSIQPSWDEYYQALLEELKQQAQAYLERLTGELRENGIETEFRMEVS
DNIPETILSTAEDINASLISFTTHNRSGIEKWYYGSVAAGLIENSPRPMLLWRNSI

Sequences:

>Translated_296_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGIKSSGK
MFCGELLPLILKCIRDNDIDLLCVASHGYTGIKKMVLGSVSEKLIKSTNLPVLLVKDGYDADKAAFGNILLPLDGSLFSE
ACLKQVSVLARDFKSKLTLLVVIQPPCIPSDRSPSIQPSWDEYYQALLEELKQQAQAYLERLTGELRENGIETEFRMEVS
DNIPETILSTAEDINASLISFTTHNRSGIEKWYYGSVAAGLIENSPRPMLLWRNSI
>Mature_296_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGIKSSGK
MFCGELLPLILKCIRDNDIDLLCVASHGYTGIKKMVLGSVSEKLIKSTNLPVLLVKDGYDADKAAFGNILLPLDGSLFSE
ACLKQVSVLARDFKSKLTLLVVIQPPCIPSDRSPSIQPSWDEYYQALLEELKQQAQAYLERLTGELRENGIETEFRMEVS
DNIPETILSTAEDINASLISFTTHNRSGIEKWYYGSVAAGLIENSPRPMLLWRNSI

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788173, Length=74, Percent_Identity=52.7027027027027, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 32970; Mature: 32970

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDI
CCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
GKELRSEYVLQVKGIKSSGKMFCGELLPLILKCIRDNDIDLLCVASHGYTGIKKMVLGSV
HHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
SEKLIKSTNLPVLLVKDGYDADKAAFGNILLPLDGSLFSEACLKQVSVLARDFKSKLTLL
HHHHHHHCCCCEEEEECCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
VVIQPPCIPSDRSPSIQPSWDEYYQALLEELKQQAQAYLERLTGELRENGIETEFRMEVS
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DNIPETILSTAEDINASLISFTTHNRSGIEKWYYGSVAAGLIENSPRPMLLWRNSI
CCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDI
CCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
GKELRSEYVLQVKGIKSSGKMFCGELLPLILKCIRDNDIDLLCVASHGYTGIKKMVLGSV
HHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
SEKLIKSTNLPVLLVKDGYDADKAAFGNILLPLDGSLFSEACLKQVSVLARDFKSKLTLL
HHHHHHHCCCCEEEEECCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
VVIQPPCIPSDRSPSIQPSWDEYYQALLEELKQQAQAYLERLTGELRENGIETEFRMEVS
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DNIPETILSTAEDINASLISFTTHNRSGIEKWYYGSVAAGLIENSPRPMLLWRNSI
CCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA