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The map label for this gene is purD [H]

Identifier: 57234407

GI number: 57234407

Start: 767278

End: 768555

Strand: Direct

Name: purD [H]

Synonym: DET0838

Alternate gene names: 57234407

Gene position: 767278-768555 (Clockwise)

Preceding gene: 57234408

Following gene: 57234406

Centisome position: 52.21

GC content: 49.14

Gene sequence:

>1278_bases
CTGAAAGTTCTGGTTATTGGAAATGGGGCAAGAGAGCATGCTATTGTCTGGAAGCTCTCTCAGAGCCCGAAAGTTACTAA
AATTTACGTTGCCCCGGGTAATGCCGGTACAAGCCTTATAGCTGAAAATGTGGCTATAAGCTGTACAAATATCAAAGGTT
TGCTGGCCTTCGCCAAAGATAACTCCATTGATATCACTTTTGTAGGGCCTGAAGCACCGCTGGCAGGCGGAGTGGTAGAT
GTCTTTGCATCTGCCGGTCTGCGCATATTCGGACCCTGCCGTGAGGCAGCCCAGCTGGAATCAAGCAAGGTTTTTACCAA
AGAACTGCTTCTTTCAAATAAAATCCCCACCGCTTACAGCCGCAGTTTTTCCAGTTATGAAAAAGCTTGTTCATATTTGA
ACCGGCTTGAAATGCCGGTGGTAATCAAGGCAGACGGTCTGGCAGGCGGCAAAGGGGTTACCGTTGCCCAAACCTATGAG
CAGGCTATGGCGGCCTTATCAGATATTATGGAAGCCAAAATATTCGGCGAAGCCGGCGAAAACGTAGTTATAGAAGAGTG
CATGGTGGGGCGTGAGATGAGCTTTTTTGCGGTTACTGACGGCAAAACGGTTGCTCCTTTAAGTGCCGCCTGTGATTATA
AACGGATTTATGACGGGGATAAGGGTGCTAATACCGGCGGTATGGGCAGTTATACCCCCCCCGTGTTTTTTACACCTGAG
CTTAAGGACGAGATAATGTCCCGTATTCTGCTCCCGGTTATCAAAGCCCTGGCTGAACGCGGTATTACCTATAAAGGTGT
TTTATATGCGGGGCTGATGATGACAGCCGAAGGGCCAAAGGTCATGGAGTTTAATGCCCGGTTTGGAGACCCCGAAACAC
AGGTTTTGATGCCCCTGCTTAAAACAGACTTGCTTGAAATAGTGGAGGCAACCCTGGAGGGGCGGCTTGATAAACTTAGT
ATTGAAATGGAAGACTCCTGCGCCGTGGGAGTAGTCTTGGCTTCAGGCGGTTACCCGGCTGAATACCGCAAGAGATTGCC
CATAAGCGGCCTTTCAGATATGCCTTCGGATATACTGGTCTTTCATGCCGGCACTGTTGTTTCTGAAGAGGATGAGATAG
TTACCAATGGCGGGAGGGTACTCTGTCTGGTCAGCAAAGGCCAAAGCATAGAGGCTGCCCGGGAAAATGTATATAGGAAC
ATAGGCAAGGTATCTTTTGAAGATTGCCAGTACCGCCGGGACATAGCCTTGTTTAACGGGAGGGAACTATGCCGCTAG

Upstream 100 bases:

>100_bases
TTCCTGAGGGAAGACGGCAATTATGAGTTTTATGCCCCCCGTTGGCATGGCCGCTACGAGCGGGGCAGAAGAATAGTTTA
GTTTTGCTGAGGAGACAACT

Downstream 100 bases:

>100_bases
TAAGTATTGTAATGGGCTCTAAATCTGATGCCGAGGTTATGAAACCTACTGAGGAAATACTGGAGAAAATGGGCATTTCT
TTTGAGACGCTGGTTATGTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKVLVIGNGAREHAIVWKLSQSPKVTKIYVAPGNAGTSLIAENVAISCTNIKGLLAFAKDNSIDITFVGPEAPLAGGVVD
VFASAGLRIFGPCREAAQLESSKVFTKELLLSNKIPTAYSRSFSSYEKACSYLNRLEMPVVIKADGLAGGKGVTVAQTYE
QAMAALSDIMEAKIFGEAGENVVIEECMVGREMSFFAVTDGKTVAPLSAACDYKRIYDGDKGANTGGMGSYTPPVFFTPE
LKDEIMSRILLPVIKALAERGITYKGVLYAGLMMTAEGPKVMEFNARFGDPETQVLMPLLKTDLLEIVEATLEGRLDKLS
IEMEDSCAVGVVLASGGYPAEYRKRLPISGLSDMPSDILVFHAGTVVSEEDEIVTNGGRVLCLVSKGQSIEAARENVYRN
IGKVSFEDCQYRRDIALFNGRELCR

Sequences:

>Translated_425_residues
MKVLVIGNGAREHAIVWKLSQSPKVTKIYVAPGNAGTSLIAENVAISCTNIKGLLAFAKDNSIDITFVGPEAPLAGGVVD
VFASAGLRIFGPCREAAQLESSKVFTKELLLSNKIPTAYSRSFSSYEKACSYLNRLEMPVVIKADGLAGGKGVTVAQTYE
QAMAALSDIMEAKIFGEAGENVVIEECMVGREMSFFAVTDGKTVAPLSAACDYKRIYDGDKGANTGGMGSYTPPVFFTPE
LKDEIMSRILLPVIKALAERGITYKGVLYAGLMMTAEGPKVMEFNARFGDPETQVLMPLLKTDLLEIVEATLEGRLDKLS
IEMEDSCAVGVVLASGGYPAEYRKRLPISGLSDMPSDILVFHAGTVVSEEDEIVTNGGRVLCLVSKGQSIEAARENVYRN
IGKVSFEDCQYRRDIALFNGRELCR
>Mature_425_residues
MKVLVIGNGAREHAIVWKLSQSPKVTKIYVAPGNAGTSLIAENVAISCTNIKGLLAFAKDNSIDITFVGPEAPLAGGVVD
VFASAGLRIFGPCREAAQLESSKVFTKELLLSNKIPTAYSRSFSSYEKACSYLNRLEMPVVIKADGLAGGKGVTVAQTYE
QAMAALSDIMEAKIFGEAGENVVIEECMVGREMSFFAVTDGKTVAPLSAACDYKRIYDGDKGANTGGMGSYTPPVFFTPE
LKDEIMSRILLPVIKALAERGITYKGVLYAGLMMTAEGPKVMEFNARFGDPETQVLMPLLKTDLLEIVEATLEGRLDKLS
IEMEDSCAVGVVLASGGYPAEYRKRLPISGLSDMPSDILVFHAGTVVSEEDEIVTNGGRVLCLVSKGQSIEAARENVYRN
IGKVSFEDCQYRRDIALFNGRELCR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=421, Percent_Identity=45.8432304038005, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI4503915, Length=421, Percent_Identity=45.8432304038005, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=421, Percent_Identity=45.8432304038005, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=421, Percent_Identity=45.8432304038005, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=419, Percent_Identity=50.1193317422434, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567511, Length=420, Percent_Identity=40.2380952380952, Blast_Score=296, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=435, Percent_Identity=42.9885057471264, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24582400, Length=427, Percent_Identity=44.2622950819672, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI62471619, Length=427, Percent_Identity=44.2622950819672, Blast_Score=332, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45811; Mature: 45811

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGNGAREHAIVWKLSQSPKVTKIYVAPGNAGTSLIAENVAISCTNIKGLLAFAKD
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCHHEECCEEEEEECCEEEEEEECC
NSIDITFVGPEAPLAGGVVDVFASAGLRIFGPCREAAQLESSKVFTKELLLSNKIPTAYS
CCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHH
RSFSSYEKACSYLNRLEMPVVIKADGLAGGKGVTVAQTYEQAMAALSDIMEAKIFGEAGE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVVIEECMVGREMSFFAVTDGKTVAPLSAACDYKRIYDGDKGANTGGMGSYTPPVFFTPE
CEEHHHHHCCCCCEEEEEECCCEECCHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEECCH
LKDEIMSRILLPVIKALAERGITYKGVLYAGLMMTAEGPKVMEFNARFGDPETQVLMPLL
HHHHHHHHHHHHHHHHHHHCCCEEEHHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
KTDLLEIVEATLEGRLDKLSIEMEDSCAVGVVLASGGYPAEYRKRLPISGLSDMPSDILV
HHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCEEE
FHAGTVVSEEDEIVTNGGRVLCLVSKGQSIEAARENVYRNIGKVSFEDCQYRRDIALFNG
EECCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCEEEECC
RELCR
HHHCC
>Mature Secondary Structure
MKVLVIGNGAREHAIVWKLSQSPKVTKIYVAPGNAGTSLIAENVAISCTNIKGLLAFAKD
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCHHEECCEEEEEECCEEEEEEECC
NSIDITFVGPEAPLAGGVVDVFASAGLRIFGPCREAAQLESSKVFTKELLLSNKIPTAYS
CCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHH
RSFSSYEKACSYLNRLEMPVVIKADGLAGGKGVTVAQTYEQAMAALSDIMEAKIFGEAGE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVVIEECMVGREMSFFAVTDGKTVAPLSAACDYKRIYDGDKGANTGGMGSYTPPVFFTPE
CEEHHHHHCCCCCEEEEEECCCEECCHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEECCH
LKDEIMSRILLPVIKALAERGITYKGVLYAGLMMTAEGPKVMEFNARFGDPETQVLMPLL
HHHHHHHHHHHHHHHHHHHCCCEEEHHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
KTDLLEIVEATLEGRLDKLSIEMEDSCAVGVVLASGGYPAEYRKRLPISGLSDMPSDILV
HHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCEEE
FHAGTVVSEEDEIVTNGGRVLCLVSKGQSIEAARENVYRNIGKVSFEDCQYRRDIALFNG
EECCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCEEEECC
RELCR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]