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The map label for this gene is purB [H]

Identifier: 57234405

GI number: 57234405

Start: 769007

End: 770362

Strand: Direct

Name: purB [H]

Synonym: DET0840

Alternate gene names: 57234405

Gene position: 769007-770362 (Clockwise)

Preceding gene: 57234406

Following gene: 57234404

Centisome position: 52.32

GC content: 48.01

Gene sequence:

>1356_bases
ATGATAGAGCGATACAGCCGCCCTCAAATGAAAAAAGTCTGGTCAGACGAAAGCAAATTCGGTTACTGGCTGGATATTGA
AATTGCAGTCTGCGAAGCCTGGGCTAAAATTGGCGTGATTTCCCGTGAGGATATTACCAAAATCAAGCTGGCGCGTTTGA
ATTTCAAACGTATGGAAGAACTACTAAAAGAAACCCACCACGATATGACTGCCTTTCTGGGCTCGGTGGCCGAAAGCTTG
GGTGATGAATCCCGCTTTATCCATATGGGCATGACTTCTTCAGACGTTATGGACACCGCCCTCAGCCTTCAGCTGGTCGA
AGCCTCCAAGATACTTAACAGCGGCATTAAAGAGCTGATAAATGCCCTGGCTGCCAAAGCTATGGAGTATAAATATACTG
TTCAGGTAGGGCGTACCCACGGCGTGCACGCCGAACCCATTTCATTCGGGCTGAAACTGGCACTCTGGATGGAAGAAATG
AAGCGTAACCGCCAGCGCCTTGCGGACGCCACCAAAGCTATTACGGTGGGCAAAATGTCAGGTGCGGTAGGCACATATGC
TACTTTATCACCCGAAATTGAAGAAATAGCCTGTAAAAAACTGGGGCTTTCCCCGGCTTCCATTTCCAATCAGGTAATCC
AGCGTGACCGTCATGCTCAGTACATGACTACTCTGGCCATTATCGCCGGTTCGCTGGAGAAATTTGCTACCGAAATACGG
GCTCTTCAGAAGACTGAATGCCACGAGGCTGAAGAACCATTTGAAAAAGGGCAAACCGGTTCGTCAGCTATGCCTCATAA
GAAAAATCCTGAGCTTTGCGAACGAATTTGCGGTATTGCCCGTATAATACGCGGTTATTCCGTTACCGCCATGGAAAATC
AGCCCTTGTGGCATGAGCGGGATATCAGCCACTCCTCTACCGAACGGGTAATAATGCCTGACGGCTGTCTGCTGCTGGAT
TACGCTTTGCACATTTTTACCAATGTTATAAAGGGTCTAAATGTTTTCCCTGAACAGATGGAAAAGAATCTTAACCTTAC
CGGCGGTCTGGTTTATTCACAGAGAGTTATGCTTGCCCTGATAAACAAGGGGCTTTCACGCCAGCAGGCATACAAGATGG
TGCAGCGGAATGCTATGCGCACCTGGCAAGGCGAAGATAATTTTATGAACCTGCTCAAGGCGGATACCGAGGTTATGGAA
CACCTTTCTTCTGCCGAAGTTGACGAATTATTTGACTACAAATTTTATCTGCGCTACATAGATGATATATTCAGACGGGT
AGGGCTGACTAATTCCCAGTGGAAGAAAGGCGGGGATGCCTCCTCTGACGAAGGACTGGCCCCCAGAGCTATATAA

Upstream 100 bases:

>100_bases
AGAATGCGGCTTATCTGGCGGCTGAAATACTGGGTTTAAAATATCAAAATATCCGTCAGGCTTATGAAAGCTACCGTCGG
GAACTAAAAGGTGAGAACAA

Downstream 100 bases:

>100_bases
ATGACTGATAACAAAGTACTCATGGAAACCGCCCTGCCGCTGCCTTCTTTTATTAAGGGTAAGGTGCGGGATACTTATCT
GCTGGGTGATAAACTGCTTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIERYSRPQMKKVWSDESKFGYWLDIEIAVCEAWAKIGVISREDITKIKLARLNFKRMEELLKETHHDMTAFLGSVAESL
GDESRFIHMGMTSSDVMDTALSLQLVEASKILNSGIKELINALAAKAMEYKYTVQVGRTHGVHAEPISFGLKLALWMEEM
KRNRQRLADATKAITVGKMSGAVGTYATLSPEIEEIACKKLGLSPASISNQVIQRDRHAQYMTTLAIIAGSLEKFATEIR
ALQKTECHEAEEPFEKGQTGSSAMPHKKNPELCERICGIARIIRGYSVTAMENQPLWHERDISHSSTERVIMPDGCLLLD
YALHIFTNVIKGLNVFPEQMEKNLNLTGGLVYSQRVMLALINKGLSRQQAYKMVQRNAMRTWQGEDNFMNLLKADTEVME
HLSSAEVDELFDYKFYLRYIDDIFRRVGLTNSQWKKGGDASSDEGLAPRAI

Sequences:

>Translated_451_residues
MIERYSRPQMKKVWSDESKFGYWLDIEIAVCEAWAKIGVISREDITKIKLARLNFKRMEELLKETHHDMTAFLGSVAESL
GDESRFIHMGMTSSDVMDTALSLQLVEASKILNSGIKELINALAAKAMEYKYTVQVGRTHGVHAEPISFGLKLALWMEEM
KRNRQRLADATKAITVGKMSGAVGTYATLSPEIEEIACKKLGLSPASISNQVIQRDRHAQYMTTLAIIAGSLEKFATEIR
ALQKTECHEAEEPFEKGQTGSSAMPHKKNPELCERICGIARIIRGYSVTAMENQPLWHERDISHSSTERVIMPDGCLLLD
YALHIFTNVIKGLNVFPEQMEKNLNLTGGLVYSQRVMLALINKGLSRQQAYKMVQRNAMRTWQGEDNFMNLLKADTEVME
HLSSAEVDELFDYKFYLRYIDDIFRRVGLTNSQWKKGGDASSDEGLAPRAI
>Mature_451_residues
MIERYSRPQMKKVWSDESKFGYWLDIEIAVCEAWAKIGVISREDITKIKLARLNFKRMEELLKETHHDMTAFLGSVAESL
GDESRFIHMGMTSSDVMDTALSLQLVEASKILNSGIKELINALAAKAMEYKYTVQVGRTHGVHAEPISFGLKLALWMEEM
KRNRQRLADATKAITVGKMSGAVGTYATLSPEIEEIACKKLGLSPASISNQVIQRDRHAQYMTTLAIIAGSLEKFATEIR
ALQKTECHEAEEPFEKGQTGSSAMPHKKNPELCERICGIARIIRGYSVTAMENQPLWHERDISHSSTERVIMPDGCLLLD
YALHIFTNVIKGLNVFPEQMEKNLNLTGGLVYSQRVMLALINKGLSRQQAYKMVQRNAMRTWQGEDNFMNLLKADTEVME
HLSSAEVDELFDYKFYLRYIDDIFRRVGLTNSQWKKGGDASSDEGLAPRAI

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=27.6923076923077, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.1984334203655, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1790398, Length=385, Percent_Identity=24.9350649350649, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787376, Length=298, Percent_Identity=26.1744966442953, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787896, Length=213, Percent_Identity=26.2910798122066, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=401, Percent_Identity=27.930174563591, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=335, Percent_Identity=28.955223880597, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=27.5252525252525, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324993, Length=201, Percent_Identity=31.8407960199005, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=25.7425742574257, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI78710009, Length=317, Percent_Identity=23.9747634069401, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640177, Length=212, Percent_Identity=27.3584905660377, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640179, Length=212, Percent_Identity=27.3584905660377, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24662684, Length=271, Percent_Identity=24.7232472324723, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50969; Mature: 50969

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPQMKKVWSDESKFGYWLDIEIAVCEAWAKIGVISREDITKIKLARLNFKRMEE
CCCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHH
LLKETHHDMTAFLGSVAESLGDESRFIHMGMTSSDVMDTALSLQLVEASKILNSGIKELI
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALAAKAMEYKYTVQVGRTHGVHAEPISFGLKLALWMEEMKRNRQRLADATKAITVGKMS
HHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GAVGTYATLSPEIEEIACKKLGLSPASISNQVIQRDRHAQYMTTLAIIAGSLEKFATEIR
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQKTECHEAEEPFEKGQTGSSAMPHKKNPELCERICGIARIIRGYSVTAMENQPLWHER
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DISHSSTERVIMPDGCLLLDYALHIFTNVIKGLNVFPEQMEKNLNLTGGLVYSQRVMLAL
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
INKGLSRQQAYKMVQRNAMRTWQGEDNFMNLLKADTEVMEHLSSAEVDELFDYKFYLRYI
HHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DDIFRRVGLTNSQWKKGGDASSDEGLAPRAI
HHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIERYSRPQMKKVWSDESKFGYWLDIEIAVCEAWAKIGVISREDITKIKLARLNFKRMEE
CCCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHH
LLKETHHDMTAFLGSVAESLGDESRFIHMGMTSSDVMDTALSLQLVEASKILNSGIKELI
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALAAKAMEYKYTVQVGRTHGVHAEPISFGLKLALWMEEMKRNRQRLADATKAITVGKMS
HHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GAVGTYATLSPEIEEIACKKLGLSPASISNQVIQRDRHAQYMTTLAIIAGSLEKFATEIR
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQKTECHEAEEPFEKGQTGSSAMPHKKNPELCERICGIARIIRGYSVTAMENQPLWHER
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DISHSSTERVIMPDGCLLLDYALHIFTNVIKGLNVFPEQMEKNLNLTGGLVYSQRVMLAL
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
INKGLSRQQAYKMVQRNAMRTWQGEDNFMNLLKADTEVMEHLSSAEVDELFDYKFYLRYI
HHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DDIFRRVGLTNSQWKKGGDASSDEGLAPRAI
HHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]